Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,165,872 +C 100% intergenic (‑177/‑207) RCAP_rcc02007 ← / → tig phage integrase/trigger factor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,165,8721.→C100.0% 627.6 165intergenic (‑177/‑207)RCAP_rcc02007/tigphage integrase/trigger factor
Reads supporting (aligned to +/- strand):  new base (84/80):  ref base (1/0):  total (85/80)

Only 100 of 186 total aligned reads displayed.

GGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTT.C.TCTG.CCTG.CCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGAAAA  >  NC_014034/2165777‑2165955
                                                                                                   |                                                                                   
ggcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccc                                                                                  >  2:1977868/1‑101 (MQ=255)
ggcgTTGAGATTTCCCGGGGCGAAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccc                                                                                  <  1:484881/101‑1 (MQ=255)
 gcgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.cccc                                                                                 >  2:2154744/1‑100 (MQ=255)
  cgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccccg                                                                                <  2:3653944/101‑3 (MQ=255)
  cgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccccg                                                                                <  1:4609012/101‑3 (MQ=255)
  cgTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccccg                                                                                <  2:4687233/101‑3 (MQ=255)
   gTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccccgc                                                                               >  1:1093634/1‑98 (MQ=255)
   gTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccccgc                                                                               <  2:3630142/101‑4 (MQ=255)
     tGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTG.ccccgcgc                                                                             >  2:2257663/1‑96 (MQ=255)
       agaTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTg                                                                            >  2:2507195/1‑101 (MQ=255)
        gaTTTCCCGGGGCGCAGAGTCAACCTGAGACGACATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGc                                                                           >  1:1700948/1‑101 (MQ=255)
         aTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGcc                                                                          <  2:3892126/101‑1 (MQ=255)
         aTTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGcc                                                                          >  1:856145/1‑101 (MQ=255)
          tttCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCg                                                                         >  1:3923483/1‑101 (MQ=255)
          tttCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGAACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCg                                                                         <  1:226277/101‑1 (MQ=255)
            tCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGtc                                                                       <  1:4091104/101‑1 (MQ=255)
             cccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGtct                                                                      >  2:4498264/1‑101 (MQ=255)
              ccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGtctc                                                                     <  1:106154/101‑1 (MQ=255)
              ccGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGtctc                                                                     <  2:4398745/101‑1 (MQ=255)
               cGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCg                                                                    >  1:2458234/1‑101 (MQ=255)
                ggggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCgg                                                                   >  1:221288/1‑101 (MQ=255)
                  ggCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCgggg                                                                 >  1:1548179/1‑101 (MQ=255)
                    cgcAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCg                                                               >  1:4114612/1‑101 (MQ=255)
                     gcAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgt                                                              >  2:3553128/1‑101 (MQ=255)
                      cAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtc                                                             >  2:1380627/1‑101 (MQ=255)
                       agagTCAACCTGAGCCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtct                                                            <  2:2589892/101‑1 (MQ=255)
                       agagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtct                                                            >  1:4462022/1‑101 (MQ=255)
                       agagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCggct                                                            >  2:1717226/1‑101 (MQ=255)
                       agagTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGAACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtct                                                            >  2:489319/1‑101 (MQ=255)
                         agTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgt                                                          >  1:3561825/1‑101 (MQ=255)
                         agTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgt                                                          <  2:4216163/101‑1 (MQ=255)
                         agTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgt                                                          <  1:1368795/101‑1 (MQ=255)
                           tCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtct                                                        <  1:321540/101‑1 (MQ=255)
                           tCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCgtctgtct                                                        <  1:117051/101‑1 (MQ=255)
                            cAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGtctgtctg                                                       <  2:725744/101‑1 (MQ=255)
                            cAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGtctgtctg                                                       >  2:421075/1‑101 (MQ=255)
                            cAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGtctgtctg                                                       >  2:1692339/1‑101 (MQ=255)
                               ccTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGc                                                    <  2:3373160/101‑1 (MQ=255)
                               ccTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGc                                                    <  1:771493/101‑1 (MQ=255)
                               ccTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGc                                                    <  1:3786122/101‑1 (MQ=255)
                               ccTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGc                                                    >  1:3952326/1‑101 (MQ=255)
                                cTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCg                                                   >  2:1762424/1‑101 (MQ=255)
                                 tGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGa                                                  <  1:2615245/101‑1 (MQ=255)
                                  gagaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGaa                                                 >  2:1403967/1‑101 (MQ=255)
                                  gagaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGaa                                                 <  2:1175422/101‑1 (MQ=255)
                                   agaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAAt                                                >  1:3726243/1‑101 (MQ=255)
                                    gaCGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATc                                               <  2:3010061/101‑1 (MQ=255)
                                      cGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCtt                                             <  1:464040/101‑1 (MQ=255)
                                        gCATGCAACACGGCACTGGTGCAGCCCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCCCGGGGCGTCTGTCTGGGCGAATCtttt                                           <  2:4418433/101‑1 (MQ=255)
                                        gCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCtttt                                           >  1:158191/1‑101 (MQ=255)
                                         cATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTc                                          <  1:3420307/101‑1 (MQ=255)
                                           tGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGa                                        >  1:1927379/1‑101 (MQ=255)
                                             cAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATc                                      >  2:1532664/1‑101 (MQ=255)
                                              aaCACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCt                                     >  1:4488585/1‑101 (MQ=255)
                                              aaCACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGCCTGGGCGAATCTTTTCGATCt                                     >  1:731070/1‑101 (MQ=255)
                                                cacGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCttt                                   <  2:1703883/101‑1 (MQ=255)
                                                 acGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTc                                  <  2:1946797/101‑1 (MQ=255)
                                                  cGGCACTGGTGCAGCCCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCt                                 <  2:3868337/101‑1 (MQ=255)
                                                  cGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCt                                 <  1:1419831/101‑1 (MQ=255)
                                                  cGGCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCt                                 <  1:3655382/101‑1 (MQ=255)
                                                   ggCACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTg                                <  1:2154868/101‑1 (MQ=255)
                                                     cACTGGTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGcc                              >  2:2743347/1‑101 (MQ=255)
                                                          gTGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGcc                         <  1:2830504/101‑1 (MQ=255)
                                                           tGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCg                        <  2:1794353/101‑1 (MQ=255)
                                                           tGCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCg                        >  1:2740863/1‑101 (MQ=255)
                                                            gCAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCgg                       >  2:3236636/1‑101 (MQ=255)
                                                             cAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCggg                      <  1:2097551/101‑1 (MQ=255)
                                                             cAGACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCggg                      >  1:2776801/1‑101 (MQ=255)
                                                               gACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAc                    <  2:1664084/101‑1 (MQ=255)
                                                               gACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAc                    >  2:1272466/1‑101 (MQ=255)
                                                               gACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAc                    <  1:2168274/101‑1 (MQ=255)
                                                               gACCACCCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAc                    >  1:1882738/1‑101 (MQ=255)
                                                                accaccCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGAcc                   <  1:2442401/101‑1 (MQ=255)
                                                                 ccaccCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCg                  >  2:600232/1‑101 (MQ=255)
                                                                  caccCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGa                 >  2:2986332/1‑101 (MQ=255)
                                                                   ccccGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaa                <  2:3918965/100‑1 (MQ=255)
                                                                   accCGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaa                >  2:3259642/1‑101 (MQ=255)
                                                                     ccGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGaaaa              >  2:833834/1‑101 (MQ=255)
                                                                      cGCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAAc             <  1:4365902/101‑1 (MQ=255)
                                                                       gCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACg            <  1:566348/101‑1 (MQ=255)
                                                                       gCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACg            >  1:1305386/1‑101 (MQ=255)
                                                                       gCACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACg            >  1:2657536/1‑101 (MQ=255)
                                                                        cACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACgg           >  2:4136438/1‑101 (MQ=255)
                                                                        cACCATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACgg           <  1:1602244/101‑1 (MQ=255)
                                                                           cATCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGccc        <  1:887281/101‑1 (MQ=255)
                                                                            ctCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       <  2:332656/100‑1 (MQ=255)
                                                                            aTCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGcccc       >  1:2981375/1‑100 (MQ=255)
                                                                            aTCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       <  2:3788695/101‑1 (MQ=255)
                                                                            aTCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       >  1:907464/1‑101 (MQ=255)
                                                                            aTCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCg       <  1:3062783/101‑1 (MQ=255)
                                                                             tCTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCgg      >  1:2273557/1‑101 (MQ=255)
                                                                             tCTGACCTTT.CGTCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCgg      >  2:470545/1‑101 (MQ=255)
                                                                              tgacccTTC.C.TCTGCCCTGCCCCGCGCTCCCGTCTCGGGGCGTCTGGCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    <  2:617797/97‑1 (MQ=255)
                                                                              cTGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCCGCCGCGCCGGGACCGAAAACGGCCCGGa     >  1:834202/1‑101 (MQ=255)
                                                                               tGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    >  1:3263356/1‑101 (MQ=255)
                                                                               tGACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaa    >  2:942808/1‑101 (MQ=255)
                                                                                gACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaaa   >  2:3223597/1‑101 (MQ=255)
                                                                                gACCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaaa   <  1:1977985/101‑1 (MQ=255)
                                                                                 aCCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGaaaa  <  2:2674429/101‑1 (MQ=255)
                                                                                 aCCTTTCC.TCTGCCCTGCCCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCAGGACCGAAAACGGCCCGGaaaa  >  2:816425/1‑101 (MQ=255)
                                                                                                   |                                                                                   
GGCGTTGAGATTTCCCGGGGCGCAGAGTCAACCTGAGACGGCATGCAACACGGCACTGGTGCAGACCACCCGCACCATCTGACCTTT.C.TCTG.CCTG.CCCGCGCTGCCGTCTCGGGGCGTCTGTCTGGGCGAATCTTTTCGATCTTTCTGCCGCGCCGGGACCGAAAACGGCCCGGAAAA  >  NC_014034/2165777‑2165955

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG