Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,270 +AG 100% coding (107/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2701.→A100.0% 787.0 197coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (115/80):  ref base (0/2):  total (115/82)
*NC_0140342,177,2702.→G100.0% 752.8 197coding (107/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (115/80):  ref base (0/2):  total (115/82)

Only 100 of 223 total aligned reads displayed.

CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTT..G..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177170‑2177354
                                                                                                      ||                                                                                               
cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaa                                                                                                   >  2:1902628/1‑101 (MQ=255)
cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaa                                                                                                   >  2:2088528/1‑101 (MQ=255)
cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaa                                                                                                   >  1:2982997/1‑101 (MQ=255)
cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaa                                                                                                   >  2:1359396/1‑101 (MQ=255)
 tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaa                                                                                                  >  2:4203448/1‑100 (MQ=255)
 tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaa                                                                                                  <  2:538275/101‑2 (MQ=255)
  gCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.aaaaag                                                                                                 <  2:421926/101‑3 (MQ=255)
    gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..a.ggg                                                                                            >  2:2721739/1‑100 (MQ=255)
     tttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGACGCACCTTCCTTCATCTTGCCG.AAAA..a.gggg                                                                                           >  1:2833371/1‑99 (MQ=255)
      ttCCATATCGCCATCTATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..a.ggggt                                                                                          <  2:2206895/101‑4 (MQ=255)
      ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..a.ggggt                                                                                          >  1:3114672/1‑98 (MQ=255)
       tCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..a.ggggtt                                                                                         <  1:1305507/101‑5 (MQ=255)
         cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..a.ggggttgg                                                                                       >  2:2281262/1‑95 (MQ=255)
           caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..g..gtg                                                                                   <  2:4246204/101‑2 (MQ=255)
                 cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..g..gtgtcggtc                                                                             >  2:4654868/1‑94 (MQ=255)
                  aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..g..gtgtcggtct                                                                            >  1:2238852/1‑93 (MQ=255)
                    cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.g                                                                         <  2:2977442/101‑1 (MQ=255)
                      aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggt                                                                       >  1:1215430/1‑99 (MQ=255)
                      aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggt                                                                       <  1:3135050/101‑3 (MQ=255)
                       tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtg                                                                      >  1:2507411/1‑98 (MQ=255)
                         ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgat                                                                    >  1:177280/1‑96 (MQ=255)
                         ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgat                                                                    <  1:4019510/101‑6 (MQ=255)
                          tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatc                                                                   <  1:1013688/101‑7 (MQ=255)
                           gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatca                                                                  <  1:4596401/101‑8 (MQ=255)
                           gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatca                                                                  <  1:2899011/101‑8 (MQ=255)
                            cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatcag                                                                 <  1:2088649/101‑9 (MQ=255)
                            cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatcag                                                                 <  2:453325/101‑9 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatcagac                                                               >  1:2453563/1‑91 (MQ=255)
                              cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatcagac                                                               <  1:981988/101‑11 (MQ=255)
                                cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAt.cagacag                                                             >  1:866589/1‑101 (MQ=255)
                                 aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAgg                                                            <  2:2833206/101‑1 (MQ=255)
                                  tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGc                                                           >  1:1692404/1‑101 (MQ=255)
                                   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGcc                                                          >  1:531557/1‑101 (MQ=255)
                                    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGccc                                                         >  1:2749018/1‑101 (MQ=255)
                                    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGccc                                                         <  2:3464801/101‑1 (MQ=255)
                                    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGccc                                                         >  1:10590/1‑101 (MQ=255)
                                      tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCg                                                       >  2:141201/1‑101 (MQ=255)
                                         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                    <  2:3010575/98‑1 (MQ=255)
                                         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                    >  1:117917/4‑101 (MQ=255)
                                         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                    >  1:712995/4‑101 (MQ=255)
                                           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTc                                                  <  2:2388613/100‑1 (MQ=255)
                                           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTc                                                  <  2:4365773/100‑1 (MQ=255)
                                            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                 <  2:2906737/101‑1 (MQ=255)
                                             cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCgg                                                >  1:3329157/1‑101 (MQ=255)
                                              aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.AAGACAGCCCCCGGTTTCGGc                                               >  2:2366529/1‑101 (MQ=255)
                                               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGcc                                              >  2:2687863/1‑101 (MQ=255)
                                               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGcc                                              <  2:1553524/101‑1 (MQ=255)
                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                             >  2:264548/1‑101 (MQ=255)
                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                             >  1:826915/1‑101 (MQ=255)
                                                cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                             <  1:2721893/101‑1 (MQ=255)
                                                 aGCCCCATCTCGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                           <  2:733753/101‑1 (MQ=255)
                                                  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aa                                          <  2:3135615/101‑2 (MQ=255)
                                                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagc                                        >  1:640307/1‑98 (MQ=255)
                                                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagc                                        <  1:2927020/101‑4 (MQ=255)
                                                    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagc                                        >  1:3603538/1‑98 (MQ=255)
                                                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                       <  1:45216/101‑5 (MQ=255)
                                                      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccg                                      >  1:897438/1‑96 (MQ=255)
                                                       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCGTCTGGACG.AAAAAGG.GGTT..G..GTGCCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                      <  1:4060242/101‑1 (MQ=255)
                                                       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                      >  1:3763241/1‑101 (MQ=255)
                                                        tctcGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                     <  2:856256/101‑1 (MQ=255)
                                                         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                    <  2:2173714/101‑1 (MQ=255)
                                                           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAA..G.GGTTTGGT.GT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                  <  2:1830070/101‑1 (MQ=255)
                                                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                 <  2:2748854/101‑1 (MQ=255)
                                                              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                               <  2:3916553/101‑1 (MQ=255)
                                                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              >  1:2211944/1‑101 (MQ=255)
                                                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  2:1129559/101‑1 (MQ=255)
                                                                   tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCCgccc                          >  1:3016848/1‑99 (MQ=255)
                                                                    ttGCCGAAGCACCTTCCTTCATCTTTACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                         >  1:2809050/1‑101 (MQ=255)
                                                                     tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        <  2:2453426/101‑1 (MQ=255)
                                                                     tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        >  1:588634/1‑101 (MQ=255)
                                                                     tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        >  1:476132/1‑101 (MQ=255)
                                                                      gatctaGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                       <  1:274190/96‑1 (MQ=255)
                                                                      gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                       >  2:3702380/1‑101 (MQ=255)
                                                                       ccGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      <  2:1692299/101‑1 (MQ=255)
                                                                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGTTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     <  1:1853334/101‑1 (MQ=255)
                                                                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     <  2:3281462/101‑1 (MQ=255)
                                                                         gAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                    <  1:93628/101‑1 (MQ=255)
                                                                         gAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCCCCCCGGCCAGCGCGCaaaa                    >  2:475258/1‑101 (MQ=255)
                                                                          aaGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                   >  1:3798736/1‑101 (MQ=255)
                                                                           aGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                  >  1:2520704/1‑101 (MQ=255)
                                                                             cgccttccttCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                <  1:3210118/99‑1 (MQ=255)
                                                                             cACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                >  1:2273090/1‑101 (MQ=255)
                                                                              aCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               >  1:1961567/1‑101 (MQ=255)
                                                                              aCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               >  1:4703318/1‑101 (MQ=255)
                                                                              aCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               <  2:2131878/101‑1 (MQ=255)
                                                                                cttccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  1:1256253/1‑101 (MQ=255)
                                                                                cttccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  1:3931134/1‑101 (MQ=255)
                                                                                cttccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  2:1293668/1‑101 (MQ=255)
                                                                                  tccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc          <  2:646470/101‑3 (MQ=255)
                                                                                  tccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTT.GGGGATCACACAGTCCCCGGTTTCGGCCGAAGCGGCCCCGGCCAGCGCGGAAAACGCCA.gggc          >  1:3901170/1‑99 (MQ=255)
                                                                                   ccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca         >  1:2111337/1‑98 (MQ=255)
                                                                                   ccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca         >  2:2559330/1‑98 (MQ=255)
                                                                                   ccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca         >  2:1579374/1‑98 (MQ=255)
                                                                                    cttcATCTTGACGAAAAA..GTGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCAGCCCCGGCCCGCGCGGAAAACGCCA.gggcag        <  1:2697477/101‑5 (MQ=255)
                                                                                      tcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCACGGCCAGCGCGGAAAACGCCAGGGCagag       <  1:2366662/101‑1 (MQ=255)
                                                                                       cATCTTGAGG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGCGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  2:127815/1‑101 (MQ=255)
                                                                                       cATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:1656199/1‑101 (MQ=255)
                                                                                       cATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:1639311/1‑101 (MQ=255)
                                                                                        aTCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGc     >  2:604852/1‑101 (MQ=255)
                                                                                           ttGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcccc  >  2:1105175/1‑101 (MQ=255)
                                                                                                      ||                                                                                               
CTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTT..G..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177170‑2177354

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG