Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,276 +GT 100% coding (101/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2761.→G100.0% 654.1 181coding (101/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (104/75):  ref base (0/1):  total (104/77)
*NC_0140342,177,2762.→T100.0% 679.6 181coding (101/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (104/75):  ref base (0/2):  total (104/77)

Only 100 of 197 total aligned reads displayed.

CATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177181‑2177354
                                                                                                    ||                                                                                  
caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtg                                                                                 <  2:4246204/101‑2 (MQ=255)
caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtg                                                                                 >  2:1448296/1‑100 (MQ=255)
 aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgt                                                                                >  1:646534/1‑99 (MQ=255)
 aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgt                                                                                >  1:3441920/1‑99 (MQ=255)
 aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgt                                                                                >  1:2719189/1‑99 (MQ=255)
   cGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgtcg                                                                              >  2:603949/1‑97 (MQ=255)
      cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgtcggtc                                                                           >  2:4654868/1‑94 (MQ=255)
      cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgtcggtc                                                                           >  1:2388747/1‑94 (MQ=255)
       aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgtcggtct                                                                          <  2:671242/101‑9 (MQ=255)
       aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTg..gtgtcggtct                                                                          >  1:4552492/1‑93 (MQ=255)
         cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.g                                                                         >  1:1196099/1‑101 (MQ=255)
         cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.g                                                                         <  2:2977442/101‑1 (MQ=255)
           aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggt                                                                       <  1:3135050/101‑3 (MQ=255)
            tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtg                                                                      <  1:859868/101‑4 (MQ=255)
              ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgat                                                                    >  1:177280/1‑96 (MQ=255)
               tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatc                                                                   <  1:1013688/101‑7 (MQ=255)
                gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatca                                                                  <  2:1903474/101‑8 (MQ=255)
                gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatca                                                                  <  1:2899011/101‑8 (MQ=255)
                gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatca                                                                  >  2:891599/1‑94 (MQ=255)
                 cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatcag                                                                 <  2:453325/101‑9 (MQ=255)
                 cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatcag                                                                 <  1:2088649/101‑9 (MQ=255)
                  ccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatcaga                                                                <  1:604554/101‑10 (MQ=255)
                   cGCATAGGTCGAGAGCGCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatcagac                                                               <  2:2162130/101‑11 (MQ=255)
                   cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTt.ggtgatcagac                                                               <  1:981988/101‑11 (MQ=255)
                     cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAt.cagacag                                                             >  2:2660976/1‑101 (MQ=255)
                      aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAgg                                                            <  2:2833206/101‑1 (MQ=255)
                        aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGcc                                                          >  1:531557/1‑101 (MQ=255)
                         ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGccc                                                         <  2:3464801/101‑1 (MQ=255)
                            cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCgg                                                      >  2:2697323/1‑101 (MQ=255)
                            cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCgg                                                      >  2:1896236/1‑101 (MQ=255)
                             gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGATG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGt                                                     <  1:2753810/101‑1 (MQ=255)
                              agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                    <  2:3010575/98‑1 (MQ=255)
                               gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGttt                                                   <  1:1726189/99‑1 (MQ=255)
                                agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTc                                                  <  2:2388613/100‑1 (MQ=255)
                                 ggaCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                 <  2:617652/99‑1 (MQ=255)
                                 gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                 <  2:2906737/101‑1 (MQ=255)
                                 gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                 <  2:398862/101‑1 (MQ=255)
                                  cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCgg                                                >  1:3329157/1‑101 (MQ=255)
                                   cccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                               <  2:2113927/100‑1 (MQ=255)
                                   aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                               >  1:2238419/1‑101 (MQ=255)
                                   aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.AAGACAGCCCCCGGTTTCGGc                                               >  2:2366529/1‑101 (MQ=255)
                                    ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGcc                                              <  2:1553524/101‑1 (MQ=255)
                                      aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                           >  1:3071437/1‑101 (MQ=255)
                                      aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                           <  2:2622262/101‑1 (MQ=255)
                                       gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aa                                          <  2:3135615/101‑2 (MQ=255)
                                        ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aag                                         >  1:1298642/1‑99 (MQ=255)
                                         cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagc                                        >  1:3603538/1‑98 (MQ=255)
                                         cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagc                                        <  1:2927020/101‑4 (MQ=255)
                                          ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                       <  2:3912924/101‑5 (MQ=255)
                                          ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                       >  2:2176465/1‑97 (MQ=255)
                                           cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccg                                      >  1:2316510/1‑96 (MQ=255)
                                            aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                      >  1:3763241/1‑101 (MQ=255)
                                               tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   >  2:1297280/1‑101 (MQ=255)
                                                cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                  >  2:851908/1‑101 (MQ=255)
                                                 ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                 <  2:2748854/101‑1 (MQ=255)
                                                 ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                 >  2:129348/1‑101 (MQ=255)
                                                   tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                               <  1:2509239/101‑1 (MQ=255)
                                                   tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                               <  1:4502192/101‑1 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  1:3963435/101‑1 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  2:1629364/101‑1 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  2:1129559/101‑1 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              >  2:2480396/1‑101 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  1:90141/101‑1 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  2:4380719/101‑1 (MQ=255)
                                                    cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              <  1:2194594/101‑1 (MQ=255)
                                                       cTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                           >  1:891357/1‑101 (MQ=255)
                                                        tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCCgccc                          >  1:3016848/1‑99 (MQ=255)
                                                          tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        >  1:476132/1‑101 (MQ=255)
                                                          tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        <  2:2257820/101‑1 (MQ=255)
                                                          tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        <  2:2453426/101‑1 (MQ=255)
                                                          tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        >  1:588634/1‑101 (MQ=255)
                                                          tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        >  1:2729928/1‑101 (MQ=255)
                                                           gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCCGCGCGGa                       >  2:1821383/1‑101 (MQ=255)
                                                           gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                       >  2:3702380/1‑101 (MQ=255)
                                                             cGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGTTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     <  1:1853334/101‑1 (MQ=255)
                                                             cGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     <  2:3281462/101‑1 (MQ=255)
                                                              gAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                    <  1:4026424/101‑1 (MQ=255)
                                                              gAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                    <  1:93628/101‑1 (MQ=255)
                                                              gAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCCCCCCGGCCAGCGCGCaaaa                    >  2:475258/1‑101 (MQ=255)
                                                               aaGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                   >  1:3798736/1‑101 (MQ=255)
                                                                 gCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                 <  1:797856/101‑1 (MQ=255)
                                                                  cgccttccttCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                <  1:3210118/99‑1 (MQ=255)
                                                                  cACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                >  1:1098162/1‑101 (MQ=255)
                                                                   aCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               >  1:4703318/1‑101 (MQ=255)
                                                                   aCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               >  1:1961567/1‑101 (MQ=255)
                                                                    ccttccttCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g             >  1:463341/1‑101 (MQ=255)
                                                                     cttccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  1:1256253/1‑101 (MQ=255)
                                                                     cttccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  1:3931134/1‑101 (MQ=255)
                                                                       tccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTT.GGGGATCACACAGTCCCCGGTTTCGGCCGAAGCGGCCCCGGCCAGCGCGGAAAACGCCA.gggc          >  1:3901170/1‑99 (MQ=255)
                                                                        ccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca         >  1:2111337/1‑98 (MQ=255)
                                                                         cttcATCTTGACGAAAAA..GTGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCAGCCCCGGCCCGCGCGGAAAACGCCA.gggcag        <  1:2697477/101‑5 (MQ=255)
                                                                          ttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga       >  1:224779/1‑96 (MQ=255)
                                                                           tcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag       >  1:2454500/1‑101 (MQ=255)
                                                                            cATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:1656199/1‑101 (MQ=255)
                                                                            cATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:1018811/1‑101 (MQ=255)
                                                                            cATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:1639311/1‑101 (MQ=255)
                                                                             aTCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGc     >  1:2816221/1‑101 (MQ=255)
                                                                               cTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGccc   >  1:2745300/1‑101 (MQ=255)
                                                                                ttGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcccc  >  2:1105175/1‑101 (MQ=255)
                                                                                ttGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcccc  <  2:117901/101‑1 (MQ=255)
                                                                                                    ||                                                                                  
CATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177181‑2177354

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG