Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,285 +G 100% coding (92/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2851.→G100.0% 736.0 190coding (92/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (114/75):  ref base (1/0):  total (115/75)

Only 100 of 220 total aligned reads displayed.

TCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTT..G..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAGTTGCGCATC  >  NC_014034/2177189‑2177374
                                                                                                           |                                                                                             
tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..G..GTG.TGGTCTt.g                                                                                              >  2:1473868/1‑101 (MQ=255)
  gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.gg                                                                                             >  2:334621/1‑100 (MQ=255)
   aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggt                                                                                            >  1:1215430/1‑99 (MQ=255)
    tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtg                                                                                           >  1:2507411/1‑98 (MQ=255)
    tCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtg                                                                                           <  1:859868/101‑4 (MQ=255)
      ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgat                                                                                         <  1:4019510/101‑6 (MQ=255)
       tGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatc                                                                                        <  1:1013688/101‑7 (MQ=255)
        gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatca                                                                                       <  2:1903474/101‑8 (MQ=255)
           cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTt.ggtgatcagac                                                                                    <  1:981988/101‑11 (MQ=255)
             cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAt.cagacag                                                                                  >  2:2660976/1‑101 (MQ=255)
              aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAgg                                                                                 <  2:2833206/101‑1 (MQ=255)
               tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGc                                                                                >  1:2089141/1‑101 (MQ=255)
                aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGcc                                                                               >  1:531557/1‑101 (MQ=255)
                aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGcc                                                                               >  1:3509801/1‑101 (MQ=255)
                 ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGccc                                                                              >  1:10590/1‑101 (MQ=255)
                 ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGccc                                                                              <  1:3148854/101‑1 (MQ=255)
                   tCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCg                                                                            >  2:141201/1‑101 (MQ=255)
                    cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCgg                                                                           >  2:2697323/1‑101 (MQ=255)
                     gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGATG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGt                                                                          <  1:2753810/101‑1 (MQ=255)
                      agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                                         <  2:2067030/98‑1 (MQ=255)
                      agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                                         >  1:712995/4‑101 (MQ=255)
                      agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                                         >  1:117917/4‑101 (MQ=255)
                       gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGttt                                                                        <  1:1726189/99‑1 (MQ=255)
                         gccccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                                      <  1:3960182/98‑1 (MQ=255)
                         gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                                      <  2:2906737/101‑1 (MQ=255)
                         gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                                      <  2:398862/101‑1 (MQ=255)
                          cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCgg                                                                     >  1:3329157/1‑101 (MQ=255)
                           aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                                                    >  2:2388810/1‑101 (MQ=255)
                           aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                                                    >  1:2238419/1‑101 (MQ=255)
                           aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                                                    >  1:2966930/1‑101 (MQ=255)
                            ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGcc                                                                   <  2:1553524/101‑1 (MQ=255)
                            ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGcc                                                                   >  2:2984711/1‑101 (MQ=255)
                             cAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCg                                                                  <  1:2721893/101‑1 (MQ=255)
                              aGCCCCATCTCGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                <  2:733753/101‑1 (MQ=255)
                              aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                >  1:3071437/1‑101 (MQ=255)
                              aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                <  2:2622262/101‑1 (MQ=255)
                                ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aag                                                              >  1:1298642/1‑99 (MQ=255)
                                  ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                            <  2:3912924/101‑5 (MQ=255)
                                  ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                            >  2:2176465/1‑97 (MQ=255)
                                   cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccg                                                           >  1:897438/1‑96 (MQ=255)
                                    aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCGTCTGGACG.AAAAAGG.GGTT..G..GTGCCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                           <  1:4060242/101‑1 (MQ=255)
                                    aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                           <  2:4545711/101‑1 (MQ=255)
                                     tctcGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                          <  2:856256/101‑1 (MQ=255)
                                        cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAA..G.GGTTTGGT.GT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                       <  2:1830070/101‑1 (MQ=255)
                                         ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                      <  2:2748854/101‑1 (MQ=255)
                                          gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                                     <  2:301717/101‑1 (MQ=255)
                                           tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                    >  2:4208834/1‑101 (MQ=255)
                                           tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                    <  1:4502192/101‑1 (MQ=255)
                                            cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                   <  1:2194594/101‑1 (MQ=255)
                                            cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                   >  2:2480396/1‑101 (MQ=255)
                                            cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                   <  2:4380719/101‑1 (MQ=255)
                                            cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                   <  2:1629364/101‑1 (MQ=255)
                                            cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                   <  2:1129559/101‑1 (MQ=255)
                                               cTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcg                                                >  1:891357/1‑101 (MQ=255)
                                                  tGCCGAAGCACCTTCCTTCATCTTGAGG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCTCgg                                             >  1:286264/1‑101 (MQ=255)
                                                  tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                             >  1:476132/1‑101 (MQ=255)
                                                   gatctaGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                            <  1:274190/96‑1 (MQ=255)
                                                     cGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGTTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                          <  1:1853334/101‑1 (MQ=255)
                                                     cGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                          <  1:604013/101‑1 (MQ=255)
                                                      gAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                         <  1:93628/101‑1 (MQ=255)
                                                      gAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                         >  2:2384018/1‑101 (MQ=255)
                                                      gAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCCCCCCGGCCAGCGCGCaaaa                                         >  2:475258/1‑101 (MQ=255)
                                                       aaGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                        >  1:3798736/1‑101 (MQ=255)
                                                        aGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                       >  1:2520704/1‑101 (MQ=255)
                                                        aGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                       <  1:4655124/101‑1 (MQ=255)
                                                         gCATCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                      >  2:3713225/1‑101 (MQ=255)
                                                         gCACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                      <  1:797856/101‑1 (MQ=255)
                                                          cACCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                                     >  1:2273090/1‑101 (MQ=255)
                                                           aCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                    <  2:2131878/101‑1 (MQ=255)
                                                           aCCTTCCTTCATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                    >  1:1961567/1‑101 (MQ=255)
                                                             cttccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                 >  2:1293668/1‑101 (MQ=255)
                                                             cttccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                 >  2:4527371/1‑101 (MQ=255)
                                                              ttccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                >  2:3654020/1‑100 (MQ=255)
                                                               tccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                               <  2:646470/101‑3 (MQ=255)
                                                               tccttcATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTT.GGGGATCACACAGTCCCCGGTTTCGGCCGAAGCGGCCCCGGCCAGCGCGGAAAACGCCA.gggc                               >  1:3901170/1‑99 (MQ=255)
                                                                 cttcATCTTGACGAAAAA..GTGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCAGCCCCGGCCCGCGCGGAAAACGCCA.gggcag                             <  1:2697477/101‑5 (MQ=255)
                                                                    cATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                           >  1:3125151/1‑101 (MQ=255)
                                                                    cATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                           >  1:1639311/1‑101 (MQ=255)
                                                                    cATCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                           >  1:1656199/1‑101 (MQ=255)
                                                                     aTCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                         >  1:2816221/1‑101 (MQ=255)
                                                                     aTCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                         >  2:604852/1‑101 (MQ=255)
                                                                     aTCTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                         >  1:3714748/1‑101 (MQ=255)
                                                                       cTTGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                       >  1:560467/1‑101 (MQ=255)
                                                                        ttGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                      <  2:117901/101‑1 (MQ=255)
                                                                        ttGACG.AAAAAGG.GGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.cccc                      >  2:1105175/1‑101 (MQ=255)
                                                                                      aggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc             >  2:2260576/11‑101 (MQ=255)
                                                                                      aggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc             >  1:2910600/11‑101 (MQ=255)
                                                                                      aggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc             >  1:3045234/11‑101 (MQ=255)
                                                                                       ggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa            >  2:2974024/10‑101 (MQ=255)
                                                                                        gggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg           <  1:1105261/93‑1 (MQ=255)
                                                                                        gggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg           >  1:2410988/9‑101 (MQ=255)
                                                                                          gttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGtt         >  2:2360611/7‑101 (MQ=255)
                                                                                          gttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGtt         >  1:1777600/7‑101 (MQ=255)
                                                                                           ttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGttt        >  1:1512037/6‑100 (MQ=255)
                                                                                           ttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGttt        >  1:4574624/6‑100 (MQ=255)
                                                                                            tggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGtttg       >  1:2087748/5‑99 (MQ=255)
                                                                                             ggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGtttgc      >  2:1786949/4‑98 (MQ=255)
                                                                                              gtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGtttgcg     >  1:2777074/3‑97 (MQ=255)
                                                                                               tgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGtttgcgc    >  2:3214294/2‑96 (MQ=255)
                                                                                               tgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGtttgcgc    >  1:2345426/2‑96 (MQ=255)
                                                                                                           |                                                                                             
TCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTT..G..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAGTTGCGCATC  >  NC_014034/2177189‑2177374

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG