Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,313 +A 100% coding (64/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3131.→A100.0% 894.2 224coding (64/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (131/93):  ref base (0/0):  total (131/93)

Only 100 of 248 total aligned reads displayed.

CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GGGTT..G..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAG.G.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGG  >  NC_014034/2177218‑2177405
                                                                                                           |                                                                                                
 aGCCCCATCTCGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                                                 <  2:733753/101‑1 (MQ=255)
 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                                                 >  1:3071437/1‑101 (MQ=255)
 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                                                 <  2:2622262/101‑1 (MQ=255)
  gCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aa                                                                                                >  2:515764/1‑100 (MQ=255)
    cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagc                                                                                              >  1:640307/1‑98 (MQ=255)
     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                                                             >  2:2176465/1‑97 (MQ=255)
       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCGTCTGGACG.AAAAAGGGGTT..G..GTGCCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                                                            <  1:4060242/101‑1 (MQ=255)
       aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgc                                                                                            <  2:4545711/101‑1 (MQ=255)
         ctcGGTCGCCTTTGCCGGAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                          <  2:4729168/101‑1 (MQ=255)
         ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                                                                          <  2:926351/101‑1 (MQ=255)
          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                                                         >  2:1297280/1‑101 (MQ=255)
           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAA..GGGTTTGGT.GT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                                                        <  2:1830070/101‑1 (MQ=255)
           cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                                                                        >  2:851908/1‑101 (MQ=255)
            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                                                       <  2:2748854/101‑1 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                     >  2:4208834/1‑101 (MQ=255)
              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                                                     <  1:4502192/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    <  1:90141/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    <  1:2194594/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    <  2:4380719/101‑1 (MQ=255)
               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                                                    <  2:1129559/101‑1 (MQ=255)
                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                                                   <  2:10585/101‑1 (MQ=255)
                    ttGCCGAAGCACCTTCCTTCATCTTTACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                                                                               >  1:2809050/1‑101 (MQ=255)
                     tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                                              >  1:2729928/1‑101 (MQ=255)
                       ccGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                                                                            <  2:1692299/101‑1 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                           <  1:604013/101‑1 (MQ=255)
                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                                                           <  2:3281462/101‑1 (MQ=255)
                         gAAGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                                                          >  2:2384018/1‑101 (MQ=255)
                          aaGCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                                                         >  1:384474/1‑101 (MQ=255)
                            gCATCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                                                       >  2:3713225/1‑101 (MQ=255)
                            gCACCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                                                       <  1:797856/101‑1 (MQ=255)
                             cgccttccttCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                                                                      <  1:3210118/99‑1 (MQ=255)
                              aCCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                     >  1:1961567/1‑101 (MQ=255)
                              aCCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                     >  1:4703318/1‑101 (MQ=255)
                              aCCTTCCTTCATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                                                     <  2:2131878/101‑1 (MQ=255)
                                cttccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                                                  >  1:1256253/1‑101 (MQ=255)
                                 ttccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                 >  2:3654020/1‑100 (MQ=255)
                                 ttccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                 >  1:937341/1‑100 (MQ=255)
                                 ttccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg                                                                 >  1:478160/1‑100 (MQ=255)
                                  tccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTT.GGGGATCACACAGTCCCCGGTTTCGGCCGAAGCGGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                                                >  1:3901170/1‑99 (MQ=255)
                                   ccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                               >  2:1579374/1‑98 (MQ=255)
                                   ccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                               >  1:2111337/1‑98 (MQ=255)
                                   ccttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                                               >  2:2559330/1‑98 (MQ=255)
                                     ttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                             >  1:224779/1‑96 (MQ=255)
                                     ttcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                                                             <  1:2914966/101‑6 (MQ=255)
                                      tcATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                                                             >  1:2454500/1‑101 (MQ=255)
                                       cATCTTGAGG.AAAAAGGGGTT..GGTGT..CGGTCTTGGCGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAg.g                                                           >  2:127815/1‑101 (MQ=255)
                                       cATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAg.g                                                           >  1:3125151/1‑101 (MQ=255)
                                       cATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAg.g                                                           >  1:1018811/1‑101 (MQ=255)
                                       cATCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAg.g                                                           >  1:1656199/1‑101 (MQ=255)
                                        aTCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.G.c                                                         >  1:3714748/1‑101 (MQ=255)
                                         tCTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.G.cc                                                        >  2:2296410/1‑101 (MQ=255)
                                          cTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.G.ccc                                                       >  1:2745300/1‑101 (MQ=255)
                                          cTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.G.ccc                                                       >  2:2903734/1‑101 (MQ=255)
                                          cTTGACG.AAAAAGGGGTT..GGTGT..CGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.G.ccc                                                       >  1:560467/1‑101 (MQ=255)
                                                        aggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGcc                                             >  1:3045234/11‑101 (MQ=255)
                                                         ggggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCa                                            >  2:2974024/10‑101 (MQ=255)
                                                          gggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAg                                           <  1:1105261/93‑1 (MQ=255)
                                                          gggttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAg                                           >  1:2410988/9‑101 (MQ=255)
                                                            gttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.tt                                        >  1:1777600/7‑101 (MQ=255)
                                                             ttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.ttt                                       >  1:4574624/6‑100 (MQ=255)
                                                             ttggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.ttt                                       <  1:2622650/96‑2 (MQ=255)
                                                             ttgcagt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.ttt                                       >  2:4021253/6‑100 (MQ=255)
                                                               ggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGTGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.tttgc                                     >  2:1483100/4‑98 (MQ=255)
                                                               ggtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.tttgc                                     <  1:891673/98‑4 (MQ=255)
                                                                gtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.tttgcg                                    >  1:2845692/3‑97 (MQ=255)
                                                                gtgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.tttgcg                                    >  1:1624454/3‑97 (MQ=255)
                                                                 tgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.tttgcgc                                   >  2:3214294/2‑96 (MQ=255)
                                                                 tgt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAG.tttgcgc                                   >  1:2345426/2‑96 (MQ=255)
                                                                  gt..cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGca                                   >  2:3969832/1‑101 (MQ=255)
                                                                          tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCatcatc                              >  1:4540120/11‑101 (MQ=255)
                                                                           cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCAtcatca                             <  2:2729774/92‑1 (MQ=255)
                                                                           cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCAtcatca                             >  2:195179/10‑101 (MQ=255)
                                                                           cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGCCCAGTTTGCGCAtcatca                             >  1:3940494/10‑101 (MQ=255)
                                                                            ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCAc                            >  1:4020429/9‑101 (MQ=255)
                                                                             tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACg                           <  1:1374054/94‑1 (MQ=255)
                                                                             tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACg                           >  2:4605580/8‑101 (MQ=255)
                                                                             tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACg                           <  2:1797238/94‑1 (MQ=255)
                                                                              ggagatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                          >  1:3507672/7‑101 (MQ=255)
                                                                                 gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                       >  1:3612153/4‑101 (MQ=255)
                                                                                    cagacatGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGTC.CCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                    <  2:953684/101‑1 (MQ=255)
                                                                                     agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                   >  1:1407139/1‑101 (MQ=255)
                                                                                     agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                   <  1:2281395/101‑1 (MQ=255)
                                                                                      gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                  >  1:4250894/1‑101 (MQ=255)
                                                                                      gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                  <  2:2238295/101‑1 (MQ=255)
                                                                                      gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                  >  2:3857065/1‑101 (MQ=255)
                                                                                      gacagGCCCCGGTTTCGGCCGAACCCGCCCCGGCCAGCGGGGATAACGCCAGGGCAGAG.GCCCCCAACGGGGCCAGTTTGCGCATCATCACGGTCTctcct                  <  2:3900928/101‑1 (MQ=255)
                                                                                         agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc               >  2:3059444/1‑101 (MQ=255)
                                                                                         agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc               >  2:2455682/1‑101 (MQ=255)
                                                                                          ggCCCCGTTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg              <  2:2271865/101‑1 (MQ=255)
                                                                                           gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt             <  2:3045046/101‑1 (MQ=255)
                                                                                            ccccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt            >  2:1337194/1‑101 (MQ=255)
                                                                                             cccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGttt           <  2:1639211/101‑1 (MQ=255)
                                                                                                ggTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCAc        <  2:2816056/101‑1 (MQ=255)
                                                                                                     cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg   <  2:623055/101‑1 (MQ=255)
                                                                                                     cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg   >  2:654479/1‑101 (MQ=255)
                                                                                                     cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg   >  1:2522205/1‑101 (MQ=255)
                                                                                                      ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg  >  1:2011650/1‑101 (MQ=255)
                                                                                                      ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg  >  2:1209328/1‑101 (MQ=255)
                                                                                                      ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg  <  1:2984899/101‑1 (MQ=255)
                                                                                                      ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAG.GCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg  >  1:440838/1‑101 (MQ=255)
                                                                                                           |                                                                                                
CAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..GGGTT..G..GT..CGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAG.G.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGG  >  NC_014034/2177218‑2177405

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG