Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,350 +C 100% coding (27/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,3501.→C100.0% 784.0 208coding (27/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (108/99):  ref base (0/1):  total (108/100)

Only 100 of 228 total aligned reads displayed.

CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GG.CAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444
                                                                                                      |                                                                                               
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAgg                                                                                                  >  1:1018811/1‑101 (MQ=255)
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAgg                                                                                                  >  1:3125151/1‑101 (MQ=255)
cATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAgg                                                                                                  >  1:1656199/1‑101 (MQ=255)
 aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGG.c                                                                                                >  2:604852/1‑101 (MQ=255)
 aTCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGG.c                                                                                                >  1:2816221/1‑101 (MQ=255)
   cTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGG.ccc                                                                                              >  2:2903734/1‑101 (MQ=255)
    ttGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGG.cccc                                                                                             <  2:117901/101‑1 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGcc                                                                                    >  1:3045234/11‑101 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGcc                                                                                    >  1:2910600/11‑101 (MQ=255)
              aggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGcc                                                                                    >  2:2260576/11‑101 (MQ=255)
               ggggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCa                                                                                   <  2:4552239/92‑1 (MQ=255)
                gggttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAg                                                                                  <  1:1105261/93‑1 (MQ=255)
                  gttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tt                                                                               >  2:2360611/7‑101 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.ttt                                                                              >  1:4574624/6‑100 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.ttt                                                                              <  2:3054860/96‑2 (MQ=255)
                   ttggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.ttt                                                                              <  1:2622650/96‑2 (MQ=255)
                   ttgcagtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.ttt                                                                              >  2:4021253/6‑100 (MQ=255)
                    tggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tttg                                                                             >  1:2087748/5‑99 (MQ=255)
                     ggtgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGTGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tttgc                                                                            >  2:1483100/4‑98 (MQ=255)
                       tgtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAG.tttgcgc                                                                          >  2:3214294/2‑96 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                          >  1:550661/1‑101 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                          <  2:1781800/101‑1 (MQ=255)
                        gtcggtcTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGca                                                                          >  2:3969832/1‑101 (MQ=255)
                            gtcgtggggaacagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGcatcat                                                                      <  2:3418838/91‑1 (MQ=255)
                             tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                                     >  1:4540120/11‑101 (MQ=255)
                             tcttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                                     <  1:2174046/91‑1 (MQ=255)
                             tcttggtgatcagacagGCCCCCTTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCatcatc                                                                     <  2:2982809/91‑1 (MQ=255)
                              cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                                    >  2:195179/10‑101 (MQ=255)
                              cttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCAtcatca                                                                    <  1:1979384/92‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   >  1:2369889/9‑101 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   <  1:2260709/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   <  2:1298550/93‑1 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   >  2:331494/9‑101 (MQ=255)
                               ttggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCAc                                                                   >  1:3921852/9‑101 (MQ=255)
                                tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                                  <  2:1797238/94‑1 (MQ=255)
                                tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                                  >  2:4605580/8‑101 (MQ=255)
                                tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                                  <  1:1374054/94‑1 (MQ=255)
                                tggtgatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACg                                                                  <  2:2272957/94‑1 (MQ=255)
                                 ggagatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACgg                                                                 >  1:3507672/7‑101 (MQ=255)
                                    gatcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtct                                                              <  1:3654249/98‑1 (MQ=255)
                                      tcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctct                                                            <  2:2612735/100‑1 (MQ=255)
                                      tcagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctct                                                            >  2:2322419/2‑101 (MQ=255)
                                       cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGtctctc                                                           >  2:105191/1‑101 (MQ=255)
                                        agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAGCAGGGCCAGTTTGCGCATCATCACGGTCTctcc                                                          >  1:4040008/1‑101 (MQ=255)
                                        agacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcc                                                          >  1:1407139/1‑101 (MQ=255)
                                         gacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcct                                                         >  2:3857065/1‑101 (MQ=255)
                                          acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                                        >  2:3724131/1‑101 (MQ=255)
                                          acagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTctcctc                                                        >  2:410906/1‑101 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       <  2:1624354/101‑1 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       <  2:963120/101‑1 (MQ=255)
                                           cagGCCCCGGTTTCGGCCGAAGCCCCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCtcctcc                                                       <  2:4681490/101‑1 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  2:2176391/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  2:2455682/1‑101 (MQ=255)
                                            agGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTccc                                                      >  2:2957286/1‑101 (MQ=255)
                                             ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                                     >  2:842020/1‑101 (MQ=255)
                                             ggCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCg                                                     <  2:937264/101‑1 (MQ=255)
                                              gCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGt                                                    >  2:1895361/1‑101 (MQ=255)
                                               ccccGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGtt                                                   >  2:1337194/1‑101 (MQ=255)
                                                    gTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACt                                              <  2:1215340/101‑1 (MQ=255)
                                                      ttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                            >  1:623119/1‑101 (MQ=255)
                                                      ttCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGt                                            >  2:4301177/1‑101 (MQ=255)
                                                        cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          >  1:2522205/1‑101 (MQ=255)
                                                        cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          >  1:1427950/1‑101 (MQ=255)
                                                        cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          >  2:654479/1‑101 (MQ=255)
                                                        cGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCg                                          <  2:623055/101‑1 (MQ=255)
                                                         ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                         <  1:2984899/101‑1 (MQ=255)
                                                         ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                         >  1:438568/1‑101 (MQ=255)
                                                         ggCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCgg                                         >  1:2011650/1‑101 (MQ=255)
                                                           ccgaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcc                                       >  2:3107885/5‑101 (MQ=255)
                                                             gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGcccc                                     >  1:560886/3‑101 (MQ=255)
                                                             gaaGCCGCCCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGCTTCACTGTCGGcccc                                     >  1:2434044/3‑101 (MQ=255)
                                                                gccgccCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcc                                  <  2:1511936/101‑1 (MQ=255)
                                                                 ccgccCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCcctcct                                 <  1:3876419/101‑1 (MQ=255)
                                                                  cgccCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCctcctc                                >  1:2768262/1‑101 (MQ=255)
                                                                   gccCCGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCtcctcc                               <  1:2661130/101‑1 (MQ=255)
                                                                     cccGGCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCgg                             <  2:476081/101‑1 (MQ=255)
                                                                        ggCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGc                          <  1:3150776/101‑1 (MQ=255)
                                                                         gCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         <  2:2745136/101‑1 (MQ=255)
                                                                         gCCAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         <  2:2719035/101‑1 (MQ=255)
                                                                         gCCAGCGCGGAAAACGCCA.GGTCAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCt                         >  1:4484146/1‑101 (MQ=255)
                                                                          ccAGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTc                        <  1:976791/101‑1 (MQ=255)
                                                                            aGCGCGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCgg                      <  1:1607828/101‑1 (MQ=255)
                                                                             gcgcGGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGa                     <  1:3724361/101‑1 (MQ=255)
                                                                              cgcgGAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGAc                    <  1:851982/101‑1 (MQ=255)
                                                                                cagAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaa                  >  1:4258933/3‑101 (MQ=255)
                                                                                 ggAAAACGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACaaa                 >  1:1071315/1‑101 (MQ=255)
                                                                                    aaaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc              >  1:4135482/1‑101 (MQ=255)
                                                                                    aaaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc              <  2:2808885/101‑1 (MQ=255)
                                                                                    aaaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc              >  2:2505401/1‑101 (MQ=255)
                                                                                    aaaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTc              >  1:4744885/1‑101 (MQ=255)
                                                                                     aaCGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCt             <  1:127831/101‑1 (MQ=255)
                                                                                       cGCCAGGG.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGa           <  2:1413361/101‑1 (MQ=255)
                                                                                        gccaggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc          <  2:3763006/96‑1 (MQ=255)
                                                                                        gccaggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGAc          >  1:1728339/6‑101 (MQ=255)
                                                                                          caggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCCGAccc        >  1:1800919/4‑101 (MQ=255)
                                                                                           aggg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCg       <  1:3524986/99‑1 (MQ=255)
                                                                                             gg.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAg     >  2:2067424/1‑101 (MQ=255)
                                                                                              g.CAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGt    <  1:2296543/101‑1 (MQ=255)
                                                                                                cAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   <  2:1752495/101‑1 (MQ=255)
                                                                                                cAGAGGCCCCCAACAGGGCCAGTTTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTc   <  1:3347342/101‑1 (MQ=255)
                                                                                                      |                                                                                               
CATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GG.CAGAGG.CCCCAACAGGGCCAG.TTGCGCATCATCACGGTCTCTCCTCCCGTTTCACTGTCGGCCCCTCCTCCGGGGCTCGGACAAAGTCTGACCCGAGTCG  >  NC_014034/2177257‑2177444

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG