Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,177 +C 100% coding (866/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,1771.→C100.0% 786.3 210coding (866/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (107/101):  ref base (0/0):  total (108/102)

Only 100 of 229 total aligned reads displayed.

CGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCT.GGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAT  >  NC_014034/2180079‑2180275
                                                                                                      |                                                                                                  
cGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAgg                                                                                                     >  1:1937732/1‑101 (MQ=255)
 ggCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.c                                                                                                   >  2:2252351/1‑101 (MQ=255)
  gcgtgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.cc                                                                                                  <  1:1066839/98‑2 (MQ=255)
   cATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccg                                                                                                 >  2:909587/1‑99 (MQ=255)
   cATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccg                                                                                                 >  2:3524996/1‑99 (MQ=255)
   cATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccg                                                                                                 >  1:3557937/1‑99 (MQ=255)
    aTGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccgg                                                                                                >  2:3387088/1‑98 (MQ=255)
    aTGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccgg                                                                                                >  2:2071771/1‑98 (MQ=255)
     tgtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccggt                                                                                               <  2:3371522/101‑5 (MQ=255)
      gtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccggtg                                                                                              <  2:2866955/101‑6 (MQ=255)
      gtgGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGG.ccggtg                                                                                              <  1:3201888/101‑6 (MQ=255)
        ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGTCGGTGTc                                                                                             >  2:719195/1‑101 (MQ=255)
        ggATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTc                                                                                             <  1:3170536/101‑1 (MQ=255)
            cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTcgcca                                                                                         <  2:1164117/101‑5 (MQ=255)
            cGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTcgcca                                                                                         >  1:4310880/1‑97 (MQ=255)
             gATGCCACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTcgccaa                                                                                        <  1:4737531/101‑6 (MQ=255)
               tGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGc                                                                                      >  2:2488786/1‑101 (MQ=255)
                   aCCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATgg                                                                                  <  2:101943/101‑1 (MQ=255)
                     cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCa                                                                                <  1:3882754/101‑1 (MQ=255)
                     cGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCa                                                                                >  2:3284629/1‑101 (MQ=255)
                      gTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCaa                                                                               >  2:1523280/1‑101 (MQ=255)
                       tGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAAc                                                                              >  1:676774/1‑101 (MQ=255)
                        ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                             <  1:2962038/101‑1 (MQ=255)
                        ggCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACt                                                                             >  1:2983536/1‑101 (MQ=255)
                              ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGctct                                                                       >  2:3819416/1‑101 (MQ=255)
                              ctctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGGCGCCCAGCATGGCAACTGGctct                                                                       >  1:574659/1‑101 (MQ=255)
                               tctTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTg                                                                      >  1:4577149/1‑101 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  1:3947588/1‑101 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  1:1731910/1‑101 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  2:4715444/1‑101 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    <  1:3525208/101‑1 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    <  2:4617600/101‑1 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  1:1605315/1‑101 (MQ=255)
                                 tttCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTggg                                                                    >  2:4445664/1‑101 (MQ=255)
                                    cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                 >  2:4376135/1‑101 (MQ=255)
                                    cGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCt                                                                 <  1:3079457/101‑1 (MQ=255)
                                     gTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTg                                                                >  1:3448527/1‑101 (MQ=255)
                                      tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTgg                                                               >  2:4463282/1‑101 (MQ=255)
                                      tGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGACTgg                                                               >  1:2369758/1‑101 (MQ=255)
                                       gACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGa                                                              <  1:1055434/101‑1 (MQ=255)
                                        aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                             >  1:3061115/1‑101 (MQ=255)
                                        aCCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGGCGCCAAGCATGGCAACTGGCTCTGGGCCTGGaa                                                             >  1:4049757/1‑101 (MQ=255)
                                         ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                                            <  2:4151450/101‑1 (MQ=255)
                                          cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                           >  1:243811/1‑101 (MQ=255)
                                          cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                           <  2:1670859/101‑1 (MQ=255)
                                            aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                         <  2:53128/101‑3 (MQ=255)
                                             ccccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcg                                                        <  1:4193267/101‑4 (MQ=255)
                                              cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                       >  2:1582486/1‑97 (MQ=255)
                                              cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                       <  1:4138439/101‑5 (MQ=255)
                                              cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                       <  2:2408076/101‑5 (MQ=255)
                                                  cgatcTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                  <  1:1343370/97‑3 (MQ=255)
                                                  aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                  >  1:3368635/1‑99 (MQ=255)
                                                   ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                 >  2:1758658/1‑98 (MQ=255)
                                                    gACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattcc                                                <  2:1445605/101‑5 (MQ=255)
                                                     aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                              >  1:4454033/1‑101 (MQ=255)
                                                     aCTCGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                              >  1:3767899/1‑101 (MQ=255)
                                                        cGGCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattagatcg                                            >  2:1343278/1‑93 (MQ=255)
                                                          gCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCggg                                         >  2:3679109/1‑101 (MQ=255)
                                                          gCCGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGAC.ATTCCGGCggg                                         >  1:882569/1‑101 (MQ=255)
                                                           ccGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                        >  1:3088519/1‑101 (MQ=255)
                                                           ccGTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                        >  1:2540579/1‑101 (MQ=255)
                                                             gTGGCCGATCATGTCGGCTTCGCGCTGGGG.CCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                      >  2:1438911/1‑101 (MQ=255)
                                                             gTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                      <  1:1400896/101‑1 (MQ=255)
                                                             gTGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                      >  1:2655637/1‑101 (MQ=255)
                                                              tGGCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                     <  2:3954470/101‑1 (MQ=255)
                                                                gCCGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                   <  1:693767/101‑1 (MQ=255)
                                                                 ccGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCCCGCaa                                  >  2:3638271/1‑101 (MQ=255)
                                                                 ccGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                  <  2:3795540/101‑1 (MQ=255)
                                                                 ccGATCATGTCGGCTTCGCGCG.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaa                                  >  1:2700837/1‑101 (MQ=255)
                                                                  cGATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                 >  1:1275818/1‑101 (MQ=255)
                                                                   gATCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                <  1:4240228/101‑1 (MQ=255)
                                                                    aTCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                               >  2:1609840/1‑100 (MQ=255)
                                                                     tCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                              >  2:1997181/1‑99 (MQ=255)
                                                                     tCATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaagg                              >  2:2943906/1‑99 (MQ=255)
                                                                      cATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                             <  2:223916/101‑4 (MQ=255)
                                                                      cATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                             >  1:4619315/1‑98 (MQ=255)
                                                                      cATGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggc                             >  1:948630/1‑98 (MQ=255)
                                                                       aTGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                            <  2:2983348/101‑5 (MQ=255)
                                                                        tGTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                          <  2:574608/101‑1 (MQ=255)
                                                                         gTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                         >  1:4507179/1‑101 (MQ=255)
                                                                         gTCGGCTTCGCGCT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGa                         <  1:4647620/101‑1 (MQ=255)
                                                                          tcggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAg                        <  2:3135368/101‑1 (MQ=255)
                                                                            ggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                      <  1:1020304/101‑1 (MQ=255)
                                                                            ggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                      >  1:2891336/1‑101 (MQ=255)
                                                                            ggcttcgcgcT.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggc                      >  1:1123126/1‑101 (MQ=255)
                                                                                tcgcgct.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                  <  1:2184475/97‑1 (MQ=255)
                                                                                    gct.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa              >  2:4036359/1‑101 (MQ=255)
                                                                                    gct.GGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa              <  1:2252475/101‑1 (MQ=255)
                                                                                        ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt           >  2:752023/5‑101 (MQ=255)
                                                                                        ggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAt           >  1:1842847/5‑101 (MQ=255)
                                                                                          cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc         <  1:1523381/99‑1 (MQ=255)
                                                                                          cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc         >  1:732353/3‑101 (MQ=255)
                                                                                             cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc      <  1:3267137/101‑1 (MQ=255)
                                                                                             cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc      <  1:1974901/101‑1 (MQ=255)
                                                                                             cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc      <  2:12513/101‑1 (MQ=255)
                                                                                             cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc      >  2:4233309/1‑101 (MQ=255)
                                                                                             cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc      <  2:3767664/101‑1 (MQ=255)
                                                                                              gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg     >  2:1111508/1‑101 (MQ=255)
                                                                                               aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa    <  1:2748920/101‑1 (MQ=255)
                                                                                                 cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt  >  1:1674022/1‑101 (MQ=255)
                                                                                                      |                                                                                                  
CGGCATGTGGATCGATGCGACCGTGGCCGCCTCTTTCGTGACCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCT.GGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAT  >  NC_014034/2180079‑2180275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG