Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,185,841 +C 93.5% intergenic (+114/‑391) mtlK → / → RCAP_rcc02028 mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,185,8411.→C93.5% 11.6 0.0 ‑0.792intergenic (+114/‑391)mtlK/RCAP_rcc02028mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (14/72):  ref base (1/5):  total (15/77)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.92e-01

Only 100 of 108 total aligned reads displayed.

CGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.C.CCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCGCCCCTTTC  >  NC_014034/2185742‑2185938
                                                                                                       |                                                                                                  
cGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.c                                                                                                   <  2:1544956/101‑1 (MQ=255)
cGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.c                                                                                                   <  1:3372238/101‑1 (MQ=255)
  ccccGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.c.cc                                                                                                <  1:2449343/101‑1 (MQ=255)
    ccGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.c.cccc                                                                                              <  2:1480524/101‑2 (MQ=255)
      ggAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATTTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGGGCCTC.CCG.GGGGTCC.G.CGCCCGc                                                                                             >  2:3820567/1‑101 (MQ=255)
      ggAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGGTCC.G.c.ccccgc                                                                                            >  2:4513498/1‑98 (MQ=255)
       gAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGCCGG.GGGGGCC.G.c.cccccc                                                                                            >  1:3087242/1‑98 (MQ=255)
       gAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCCGTGCCGAGGCGACCCTG.CCG.GGGGCAC.G.c.ccccccc                                                                                           >  1:3757344/1‑97 (MQ=255)
        aaCAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.c.ccccgcac                                                                                          <  2:4693812/101‑6 (MQ=255)
        aaCAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.c.ccccgcac                                                                                          <  1:616825/101‑6 (MQ=255)
        aaCAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.c.ccccgcac                                                                                          <  1:2338754/101‑6 (MQ=255)
         aCAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCAcc                                                                                          <  2:2449820/101‑1 (MQ=255)
           aGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCAcccc                                                                                        <  1:1080096/101‑1 (MQ=255)
            ggcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCAccccc                                                                                       <  2:3941452/101‑1 (MQ=255)
            ggcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCAccccc                                                                                       <  2:1217429/101‑1 (MQ=255)
             gcggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCAcccccg                                                                                      <  1:2322905/101‑2 (MQ=255)
               ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.GGGTGGGGGGC.G.C.CCCCCCcccccccc                                                                                    >  1:680517/1‑99 (MQ=255)
               ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCCAC.C.CCCGCAcccccggc                                                                                    <  2:2248099/101‑4 (MQ=39)
               ggcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCAcccccggc                                                                                    <  2:227892/101‑4 (MQ=255)
               ggcACCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCAcccccggc                                                                                    <  2:654956/101‑4 (MQ=37)
                gcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGCCGG.GGGGGCC.C.C.CCCCCCccccccccc                                                                                   >  1:4436721/1‑98 (MQ=255)
                gcCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTGCCGG.GGGGGCC.C.C.CCCCCCccccccccc                                                                                   >  2:3785375/1‑98 (MQ=255)
                  cccGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTa                                                                                <  2:666207/101‑1 (MQ=255)
                   ccGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTAt                                                                               <  1:4528118/101‑1 (MQ=255)
                     gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTAttt                                                                             <  2:4096938/101‑1 (MQ=255)
                     gCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.C.CCCCGCCCCCCCGGCGTAttt                                                                             <  1:1267867/101‑1 (MQ=255)
                       tccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTAt                                                                           >  2:1024002/1‑101 (MQ=255)
                        ccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATc                                                                          <  1:974526/101‑1 (MQ=255)
                        ccctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATc                                                                          >  2:3911772/1‑101 (MQ=255)
                         cctcccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTT.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATcc                                                                         <  2:4677483/101‑1 (MQ=25)
                           cccggggcgggGCCGCCTGATGTGCAAAGGGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCa                                                                       <  2:1707162/97‑1 (MQ=25)
                            cccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCaa                                                                      >  1:1463535/1‑101 (MQ=255)
                            cccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGGATTTATCCCaa                                                                      >  2:1623519/1‑101 (MQ=37)
                             ccGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAg                                                                     <  2:532529/101‑1 (MQ=255)
                                ggcgggGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGaaa                                                                  <  2:1672038/101‑1 (MQ=255)
                                     ggCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAagca                                                             <  1:3756377/101‑1 (MQ=255)
                                      gccgccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAagcag                                                            <  2:2758423/101‑1 (MQ=255)
                                      gccgccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAagcag                                                            >  2:274249/1‑101 (MQ=255)
                                         gccTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCgg                                                         >  2:664844/1‑101 (MQ=255)
                                           cTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCt                                                       <  2:3362085/101‑1 (MQ=255)
                                            tGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCtt                                                      <  2:3145443/101‑1 (MQ=255)
                                            tGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCtt                                                      <  1:3567629/101‑1 (MQ=255)
                                            tGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCtt                                                      <  2:1053010/101‑1 (MQ=255)
                                             gATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTg                                                     <  2:1479095/101‑1 (MQ=255)
                                              aTGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGc                                                    <  2:769543/101‑1 (MQ=255)
                                              aTGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGc                                                    <  1:1424328/101‑1 (MQ=255)
                                                 tggaacgCGTTTTCGCCGCGGCGGTGCCGAGGCGCCCCTG.CGG.GGGCTTC.G.C.CCCCGCTCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGcccc                                                 <  1:1202855/95‑1 (MQ=25)
                                                 tgCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGcccc                                                 <  1:3049837/101‑1 (MQ=255)
                                                  gCAAAGCGGTTTCGCCTCGGCGGTGCGGAGGCGACCCTG.CGG.GTGCGCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCg                                                <  2:3931737/101‑1 (MQ=37)
                                                  gCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCg                                                <  2:3304896/101‑1 (MQ=255)
                                                  gCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCg                                                <  2:680453/101‑1 (MQ=255)
                                                    aaggCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGaa                                              <  2:908150/98‑1 (MQ=37)
                                                    aaaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGaa                                              <  1:68212/101‑1 (MQ=255)
                                                    aaaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCaa                                              >  1:301161/1‑101 (MQ=37)
                                                     aaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAg                                             <  1:511413/101‑1 (MQ=255)
                                                     aaGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAg                                             <  1:520657/101‑1 (MQ=255)
                                                      aGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAgg                                            <  1:4518337/101‑1 (MQ=255)
                                                      aGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAgg                                            <  2:2272206/101‑1 (MQ=255)
                                                         ggTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGGCCCGAAGGCCt                                         >  1:589217/1‑101 (MQ=37)
                                                           tttCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTg                                       <  1:4635255/101‑1 (MQ=255)
                                                           tttCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTg                                       <  1:866747/101‑1 (MQ=255)
                                                           tttCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.CACCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGAAGCGGCTTGCCCCGAAGGCCTTg                                       <  1:3820802/101‑1 (MQ=38)
                                                            ttCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGt                                      <  2:3002683/101‑1 (MQ=255)
                                                             tCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGtt                                     <  1:1098053/101‑1 (MQ=255)
                                                               gCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGtttt                                   <  2:4436471/101‑1 (MQ=255)
                                                                  tCGGCGGTGCCGAGGCGACCCTG.CGG.GGGTTCC.G.C.CCCCCGACCCCCGGCGTATTTATCCCAAGAAAAAGCCGCGGCTTTCCCCCCAAGACTTGttttgat                                >  2:2433192/1‑98 (MQ=25)
                                                                  tCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttgat                                <  1:2828104/101‑4 (MQ=37)
                                                                   cggcggTGCCGAGGCGCCCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGttttgatg                               <  2:4168416/101‑5 (MQ=37)
                                                                     gcggTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                            <  2:3757109/101‑1 (MQ=37)
                                                                     gcggTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                            <  1:2031705/101‑1 (MQ=37)
                                                                     gcggTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGc                            <  2:2369221/101‑1 (MQ=37)
                                                                      cggTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGca                           <  1:3785610/101‑1 (MQ=37)
                                                                        gTGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGcaca                         <  1:150253/101‑1 (MQ=37)
                                                                         tGCCGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACaa                        <  2:3453478/101‑1 (MQ=37)
                                                                           ccGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGa                      <  1:1271777/101‑1 (MQ=37)
                                                                           ccGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGa                      <  2:3550323/101‑1 (MQ=37)
                                                                            cgggccGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                     <  1:6243/96‑1 (MQ=25)
                                                                            cGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTTCCCCGAAGGGCTTG.TTTTGATGGCACAAGaa                     >  2:366452/1‑101 (MQ=25)
                                                                            cGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                     <  1:145079/101‑1 (MQ=37)
                                                                            cGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                     <  1:1133577/101‑1 (MQ=37)
                                                                            cGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                     <  1:3757199/101‑1 (MQ=37)
                                                                            cGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                     <  2:1957172/101‑1 (MQ=37)
                                                                            cGAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaa                     <  1:2720896/101‑1 (MQ=37)
                                                                             gAGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGaaa                    <  1:1826079/101‑1 (MQ=37)
                                                                              aGGCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTT.ATGGCACAAAAAAc                   >  1:1673945/1‑101 (MQ=25)
                                                                              aGGCGACCCTG.CGG.GGGCTCC.G.C.CCCCGCTCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAAc                   <  1:1536581/101‑1 (MQ=37)
                                                                               ggCGACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                  <  2:4201838/101‑1 (MQ=37)
                                                                               ggCGACCCTG.CGG.GGGCTCC.G.C.CCCCGCCCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACg                  <  2:4295714/101‑1 (MQ=37)
                                                                                  gACCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCCAAGGCCGTG.TTTTGATGGCACAAGAAACGCCg               >  2:2572122/1‑101 (MQ=25)
                                                                                   aCCCTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGa              <  1:2835365/101‑1 (MQ=37)
                                                                                     ccTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAc            <  1:1285674/101‑1 (MQ=37)
                                                                                      cTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAcc           <  1:3076012/101‑1 (MQ=37)
                                                                                      cTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAcc           >  2:384652/1‑101 (MQ=37)
                                                                                      cTG.CGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAAcc           <  1:4276087/101‑1 (MQ=37)
                                                                                          cGG.GGGCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGcc        <  2:3452597/101‑1 (MQ=37)
                                                                                           gg.gggCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCCAACCGccc       >  2:1462133/1‑101 (MQ=37)
                                                                                                gCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCctt   <  2:2433821/101‑1 (MQ=37)
                                                                                                gCTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCctt   <  1:1979636/101‑1 (MQ=37)
                                                                                                 cTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCcttt  <  2:4077923/101‑2 (MQ=37)
                                                                                                 cTCC.GCC.CCCGCA.CCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCcttt  <  1:1960695/101‑2 (MQ=37)
                                                                                                       |                                                                                                  
CGCCCCGGAACAGGCGGCCCCGCTCCCTCCCGGGCGGGGCCGCCTGATGTGCAAAGCGGTTTCGCCTCGGCGGTGCCGAGGCGACCCTG.CGG.GGGCTCC.G.C.CCCGCACCCCCCGGCGTATTTATCCCAAGAAAAAGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCGCCCCTTTC  >  NC_014034/2185742‑2185938

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG