Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,280 +C 100% intergenic (‑76/‑229) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,2801.→C100.0% 788.3 208intergenic (‑76/‑229)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (95/111):  ref base (2/0):  total (97/111)

Only 100 of 240 total aligned reads displayed.

TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAG  >  NC_014034/2249180‑2249375
                                                                                                     |                                                                                                
 cGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                 >  1:3300354/1‑101 (MQ=255)
 cGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.c                                                                                                 >  2:234933/1‑101 (MQ=255)
  gAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cc                                                                                                >  2:4291921/1‑101 (MQ=255)
      aCAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccg                                                                                            >  1:285441/1‑99 (MQ=255)
       cAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgt                                                                                           >  1:3666950/1‑98 (MQ=255)
        agaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgtc                                                                                          >  1:2539308/1‑97 (MQ=255)
         gaagaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.cccccgtcc                                                                                         <  2:2990894/101‑6 (MQ=255)
           agaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcccg.tcccg                                                                                       <  2:4646164/101‑1 (MQ=255)
            gaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcccg.tcccgt                                                                                      <  1:3415515/101‑1 (MQ=255)
            gaTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCcccg.tcccgt                                                                                      <  2:1663631/101‑1 (MQ=255)
             aTCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTg                                                                                     >  2:648478/1‑101 (MQ=255)
                gCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCg                                                                                  <  1:634236/101‑1 (MQ=255)
                 ccGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCgg                                                                                 <  2:2628268/101‑1 (MQ=255)
                 ccGGACATGGTGCTCGCTTTCATTGGGGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCgg                                                                                 >  2:2774482/1‑101 (MQ=255)
                    gACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGAc                                                                              <  2:3814090/101‑1 (MQ=255)
                     aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACt                                                                             >  1:4441415/1‑101 (MQ=255)
                     aCATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACt                                                                             <  2:455776/101‑1 (MQ=255)
                      cATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTc                                                                            >  1:837133/1‑101 (MQ=255)
                        tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTccc                                                                          <  2:4434149/101‑1 (MQ=255)
                        tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTccc                                                                          >  2:2898810/1‑101 (MQ=255)
                         ggTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATATCGAAGCTTGGGCACCCGGGGCCACAAGGGGGATCTG.CCCCGTTCCCGTGCAGGGACTaccc                                                                         >  2:755928/1‑101 (MQ=255)
                           tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCg                                                                       >  2:2165213/1‑101 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCgg                                                                      >  1:4243913/1‑101 (MQ=255)
                            gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCgg                                                                      <  2:1038392/101‑1 (MQ=255)
                             cTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCggg                                                                     <  2:3269968/101‑1 (MQ=255)
                             cTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCggg                                                                     <  1:321225/101‑1 (MQ=255)
                               cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGat                                                                   <  2:468746/101‑1 (MQ=255)
                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGata                                                                  <  1:4292168/101‑1 (MQ=255)
                                gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCCTGCCGGGACTCCCCCGGGata                                                                  <  1:3498018/101‑1 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATAtt                                                                >  2:1297532/1‑101 (MQ=255)
                                  tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATAtt                                                                >  2:310025/1‑101 (MQ=255)
                                   ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATAttt                                                               >  1:3768222/1‑101 (MQ=255)
                                    tCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTg                                                              <  1:4722701/101‑1 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATAtttca                                                             >  2:4067939/1‑99 (MQ=255)
                                     cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGa                                                             >  2:1733621/1‑101 (MQ=255)
                                      aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAg                                                            >  2:3448114/1‑101 (MQ=255)
                                       ttGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAgg                                                           >  2:3643170/1‑101 (MQ=255)
                                        tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGc                                                          <  2:1181269/101‑1 (MQ=255)
                                         ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCa                                                         >  1:4536770/1‑101 (MQ=255)
                                         ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCa                                                         <  2:2113457/101‑1 (MQ=255)
                                           tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                       >  1:1853560/1‑101 (MQ=255)
                                           tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAg                                                       >  1:1224812/1‑101 (MQ=255)
                                             ttCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGAt                                                     <  2:3675426/101‑1 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    >  2:3706615/1‑101 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    >  2:3559032/1‑101 (MQ=255)
                                              tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    >  2:770067/1‑101 (MQ=255)
                                              tCTTGACATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATg                                                    <  1:777016/101‑1 (MQ=255)
                                               cTTGAGATGCACAGATACCGCAGCTTGGGTACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGa                                                   <  2:1722210/101‑1 (MQ=255)
                                                ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGaa                                                  >  2:4422794/1‑101 (MQ=255)
                                                ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGaa                                                  >  1:1634517/1‑101 (MQ=255)
                                                 tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAg                                                 <  1:3888697/101‑1 (MQ=255)
                                                  gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                >  1:305220/1‑101 (MQ=255)
                                                  gagaTGCACAGATACCGCAGCTTGGGCACCCGGAGCACGAAGGGGGCTCTGCCCCCG.CCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAgg                                                <  1:4056803/101‑1 (MQ=38)
                                                   agaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAggg                                               <  1:706662/101‑1 (MQ=255)
                                                     aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAggggg                                             >  2:2498421/1‑101 (MQ=255)
                                                        cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCt                                          >  1:3122530/1‑101 (MQ=255)
                                                        cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCt                                          >  1:3924561/1‑101 (MQ=255)
                                                        cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCt                                          >  1:4324153/1‑101 (MQ=255)
                                                         acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCtt                                         >  2:1077867/1‑101 (MQ=255)
                                                         acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCtt                                         >  1:2447788/1‑101 (MQ=255)
                                                          caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCttt                                        <  2:3351928/101‑1 (MQ=255)
                                                           aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTc                                       <  2:4503960/101‑1 (MQ=255)
                                                              tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATc                                    <  2:4323906/101‑1 (MQ=255)
                                                               aCCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCt                                   <  2:3535093/101‑1 (MQ=255)
                                                                ccGGAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                  <  2:3259628/101‑1 (MQ=255)
                                                                ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                  <  1:1730877/101‑1 (MQ=255)
                                                                ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCtt                                  <  2:2929773/101‑1 (MQ=255)
                                                                 cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTg                                 <  1:522966/101‑1 (MQ=255)
                                                                    aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCAtagatcgg                              >  2:3893807/1‑94 (MQ=37)
                                                                     gCTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGa                             >  1:2042628/1‑101 (MQ=255)
                                                                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                            >  1:1738481/1‑101 (MQ=255)
                                                                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                            >  1:4492283/1‑101 (MQ=255)
                                                                      cTTGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaa                            <  1:2093815/101‑1 (MQ=255)
                                                                        tGGGCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGCCTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGaaaa                          >  2:2854077/1‑101 (MQ=255)
                                                                         gggCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAat                         <  1:2898986/101‑1 (MQ=255)
                                                                           gcccccGGGGCAATAAGGGGGCCCTGCCCCCG.TGCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAatat                       <  1:4084169/98‑1 (MQ=37)
                                                                           gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAatat                       >  2:1573555/1‑101 (MQ=255)
                                                                           gCACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAatat                       <  1:2824843/101‑1 (MQ=255)
                                                                            cACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATc                      <  1:2507587/101‑1 (MQ=255)
                                                                            cACCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATc                      >  2:2456024/1‑101 (MQ=255)
                                                                             aCCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATcg                     <  1:1446144/101‑2 (MQ=255)
                                                                             aCCCGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATcc                     <  2:447138/101‑1 (MQ=255)
                                                                              cccGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATccc                    >  1:956930/1‑101 (MQ=255)
                                                                              cccGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATccc                    >  1:3872759/1‑101 (MQ=255)
                                                                               ccGGGGCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCg                   <  1:2174623/101‑1 (MQ=255)
                                                                                 ggggCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCggg                 <  1:909655/101‑1 (MQ=255)
                                                                                    gCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg              >  2:2081947/1‑101 (MQ=255)
                                                                                    gCAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg              <  2:219761/101‑1 (MQ=255)
                                                                                     cAAGAAGGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg             >  1:841037/1‑100 (MQ=255)
                                                                                      aagaagGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTg            >  2:4170689/1‑101 (MQ=255)
                                                                                        gaagGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa          <  1:3706845/101‑1 (MQ=255)
                                                                                        gaagGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa          <  1:3559251/101‑1 (MQ=255)
                                                                                          agGGGGCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg        <  1:2222555/101‑1 (MQ=255)
                                                                                          agGGGCCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGTATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATg        <  1:4413950/101‑1 (MQ=38)
                                                                                          agGCGGCTCTCCCCCCG.CCCCGTCCCGGGCCCCCCCCGGTATTTTGGAGGCAAGATGAGGGGGGCCTTTCTCCTTGCCAAAAATATCCCGGGGGGTGAATg        <  2:883553/101‑1 (MQ=16)
                                                                                            ggggCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc      >  2:674612/1‑101 (MQ=255)
                                                                                            ggggCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc      <  1:1297624/101‑1 (MQ=255)
                                                                                             gggCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcg     >  1:4157999/1‑101 (MQ=255)
                                                                                              ggCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc    <  1:110420/101‑1 (MQ=255)
                                                                                               gCTCTGCCCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCa   <  2:3136736/101‑1 (MQ=255)
                                                                                                     |                                                                                                
TCGAGGACAGAAGATCGCCGGACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCTG.CCCCG.TCCCGTGCCGGGACTCCCCCGGGATATTTGAGGCAAGATGAAGGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAG  >  NC_014034/2249180‑2249375

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG