Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,249,300 +C 2.6% intergenic (‑96/‑209) parE ← / → RCAP_rcc02080 DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,249,3001.→C 2.6% 10.2 ‑3.5 ‑0.0233intergenic (‑96/‑209)parE/RCAP_rcc02080DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  new base (0/6):  ref base (111/116):  total (111/122)
Fisher's exact test strand distribution p-value = 3.03e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.62e-01

Only 100 of 262 total aligned reads displayed.

ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249201‑2249400
                                                                                                       |                                                                                                       
   tGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.ccc                                                                                                      >  2:2898810/1‑101 (MQ=255)
     gTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.ccccc                                                                                                    <  2:3134959/101‑1 (MQ=255)
      tGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCg                                                                                                   <  1:2154881/101‑1 (MQ=255)
       gCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCgg                                                                                                  >  1:4149866/1‑101 (MQ=255)
         tCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGa                                                                                                <  1:3472794/101‑1 (MQ=255)
          cGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGat                                                                                               <  2:2126712/101‑1 (MQ=255)
           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              <  1:4292168/101‑1 (MQ=255)
           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              >  2:4637485/1‑101 (MQ=255)
           gCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGata                                                                                              <  1:2783405/101‑1 (MQ=255)
             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            >  2:1297532/1‑101 (MQ=255)
             tttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCCAGTCCCGTGCCGGGACT.CCCCCGGGATAtt                                                                                            >  2:2626332/1‑101 (MQ=255)
              ttCATTGGTTTTCTTGAGATGCCCAGCTACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           <  2:100963/101‑1 (MQ=255)
              ttCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATAttt                                                                                           <  2:3868649/101‑1 (MQ=255)
                cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGTTAtttta                                                                                         >  1:1396539/1‑99 (MQ=255)
                cATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGa                                                                                         >  2:1733621/1‑101 (MQ=255)
                 aTTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAg                                                                                        >  1:986291/1‑101 (MQ=255)
                   tGGTGTTCTTGAGATGCTCAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      <  2:4267512/101‑1 (MQ=255)
                   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      >  2:4301530/1‑101 (MQ=255)
                   tGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      <  2:1181269/101‑1 (MQ=255)
                   tGGTGGTCTTGAGATGCACAGATACCGCAGCTCGGGCACCCGGGGCAAGAAGGGAGCCCT.GCCCCC.GGCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGc                                                                                      <  2:2190744/101‑1 (MQ=255)
                    ggTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCa                                                                                     >  1:4536770/1‑101 (MQ=255)
                      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                   >  1:1853560/1‑101 (MQ=255)
                      tgtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAg                                                                                   >  1:1224812/1‑101 (MQ=255)
                       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAgatcgga                                                                                  >  1:2734000/1‑95 (MQ=39)
                       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  >  2:3652664/1‑101 (MQ=255)
                       gtTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGa                                                                                  >  2:3553097/1‑101 (MQ=255)
                         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                <  1:937087/101‑1 (MQ=255)
                         tCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                >  2:3706615/1‑101 (MQ=255)
                         tCTTGACATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATg                                                                                <  1:777016/101‑1 (MQ=255)
                          cTTGAGATGCACAGATACCGCAGCTTGGGTACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               <  2:1722210/101‑1 (MQ=255)
                          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               >  1:2929949/1‑101 (MQ=255)
                          cTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGa                                                                               <  1:540602/101‑1 (MQ=255)
                           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                              >  2:4422794/1‑101 (MQ=255)
                           ttGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGaa                                                                              >  1:2847885/1‑101 (MQ=255)
                            tGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...g                                                                          >  2:765073/1‑101 (MQ=255)
                             gagaTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...gg                                                                         >  1:3011174/1‑101 (MQ=255)
                                aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggggg                                                                      >  1:4033676/1‑101 (MQ=255)
                                aTGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...ggggg                                                                      >  2:2498421/1‑101 (MQ=255)
                                  gCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGc                                                                    >  1:1688696/1‑101 (MQ=255)
                                   cacaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCt                                                                   >  1:4324153/1‑101 (MQ=255)
                                    acaGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCtt                                                                  >  2:1077867/1‑101 (MQ=255)
                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 <  2:4441165/101‑1 (MQ=255)
                                     caGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCttt                                                                 <  2:3351928/101‑1 (MQ=255)
                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                <  1:3604567/101‑1 (MQ=255)
                                      aGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTc                                                                <  2:3300152/101‑1 (MQ=255)
                                         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                             <  1:3143135/101‑1 (MQ=255)
                                         tACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATc                                                             >  1:3635466/1‑101 (MQ=255)
                                           ccGGAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                           <  2:3259628/101‑1 (MQ=255)
                                           ccGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCtt                                                           <  1:1730877/101‑1 (MQ=255)
                                            cGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTg                                                          <  1:522966/101‑1 (MQ=255)
                                               aGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCAtagatcgg                                                       >  2:3893807/1‑94 (MQ=37)
                                                 cTTGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaa                                                     >  1:4492283/1‑101 (MQ=255)
                                                   tGGGCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGCCT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGaaaa                                                   >  2:2854077/1‑101 (MQ=255)
                                                    gggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAat                                                  <  1:2093568/101‑1 (MQ=255)
                                                     ggCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAata                                                 <  1:3749929/101‑1 (MQ=255)
                                                      gcccccGGGGCAATAAGGGGGCCCT.GCCCCC.GTGCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                <  1:4084169/98‑1 (MQ=37)
                                                      gCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                >  2:1573555/1‑101 (MQ=255)
                                                      gCACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAatat                                                <  2:808356/101‑1 (MQ=255)
                                                       cACCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATc                                               <  1:2507587/101‑1 (MQ=255)
                                                        aCCCGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATcc                                              <  2:447138/101‑1 (MQ=255)
                                                         cccGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATccc                                             >  1:3872759/1‑101 (MQ=255)
                                                          ccGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                            <  2:2943062/101‑1 (MQ=255)
                                                          ccGGGGCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCg                                            <  2:3666721/101‑1 (MQ=255)
                                                            ggggCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggg                                          >  1:324954/1‑101 (MQ=255)
                                                               gCAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggg                                       >  2:2081947/1‑101 (MQ=255)
                                                                cAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCggggggg                                      >  1:841037/1‑100 (MQ=255)
                                                                cAAGAAGGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGt                                      <  1:4423044/101‑1 (MQ=255)
                                                                 aagaagGGGGCTCTAC.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCgggggggg                                     >  2:4733758/1‑99 (MQ=255)
                                                                  agaagGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGGGa                                    >  2:4347125/1‑101 (MQ=255)
                                                                   gaagGGGGCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGaa                                   <  1:3559251/101‑1 (MQ=255)
                                                                     agGCGGCTCT.CCCCCC.GCCCCGTCCCGGGCCC.CCCCCGGTATTTTGGAGGCAAGATGAG...GGGGGCCTTTCTCCTTGCCAAAAATATCCCGGGGGGTGAATg                                 <  2:883553/101‑1 (MQ=16)
                                                                       ggggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                               >  2:674612/1‑101 (MQ=255)
                                                                       ggggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                               >  2:3586307/1‑101 (MQ=255)
                                                                        gggcctcgcccccc.GTCCCGTGCGGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgc                               <  2:3995811/91‑1 (MQ=37)
                                                                         ggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                             <  1:110420/101‑1 (MQ=255)
                                                                         ggCTCT.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGgcgc                             <  2:482480/101‑1 (MQ=255)
                                                                           ctct.GCCCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAg                           <  2:3472424/101‑1 (MQ=255)
                                                                              ctgccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGAAAGATGAA...GGGGGGCTTTCATCTTGCCG.AAATATCCCCGGGGGGGAATGGCGCAGCCa                        >  1:3928765/5‑101 (MQ=37)
                                                                                 ccccc.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCaga                      <  2:1794228/100‑1 (MQ=255)
                                                                                  ccgc.gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCagag                     >  1:3510041/4‑101 (MQ=255)
                                                                                   ccc.gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgg                    >  1:2206632/1‑101 (MQ=255)
                                                                                     c.gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg                  >  1:4450149/1‑101 (MQ=255)
                                                                                     c.gtcccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAgggg                  >  2:45967/1‑101 (MQ=255)
                                                                                         cccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGc               <  2:1634417/101‑1 (MQ=255)
                                                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  1:1781634/101‑1 (MQ=255)
                                                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              >  2:2215383/1‑101 (MQ=255)
                                                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              >  2:4528299/1‑101 (MQ=255)
                                                                                          ccgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGAGGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCa              <  2:3214748/101‑1 (MQ=255)
                                                                                           cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAg             >  2:2785752/1‑101 (MQ=255)
                                                                                           cgcaagcggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAATGGCGAAGCCAGAggggg                 <  1:3922950/94‑1 (MQ=18)
                                                                                             tGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcg           <  2:4149620/101‑1 (MQ=255)
                                                                                              gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcgc          >  1:233057/1‑101 (MQ=255)
                                                                                              gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAgcgc          >  1:1325977/1‑101 (MQ=255)
                                                                                                   gACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccc     <  1:737818/101‑2 (MQ=255)
                                                                                                    gcT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccct    <  2:245395/100‑3 (MQ=255)
                                                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGTGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt   <  1:2472012/101‑4 (MQ=255)
                                                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt   <  1:3889935/101‑4 (MQ=255)
                                                                                                     cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGcccccctt   <  1:3089351/101‑4 (MQ=255)
                                                                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccccttc  <  2:3059914/101‑5 (MQ=255)
                                                                                                      t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGccccccttc  <  1:1557905/101‑5 (MQ=255)
                                                                                                       |                                                                                                       
ACATGGTGCTCGCTTTCATTGGTGTTCTTGAGATGCACAGATACCGCAGCTTGGGCACCCGGGGCAAGAAGGGGGCTCT.G.CCCC.GTCCCGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGGGGCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAATGGCGCAGCCAGAGGGGGGCAGCGCCCCCTTCG  >  NC_014034/2249201‑2249400

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG