BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,249,389	+C	2.1%	intergenic (‑185/‑120)	parE ← / → RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,249,389	1	.→C	2.1%	3.8 ‑3.1 ‑0.0	193	intergenic (‑185/‑120)	parE/RCAP_rcc02080	DNA topoisomerase IV subunit B/HAD superfamily hydrolase
Reads supporting (aligned to +/- strand): new base (0/4): ref base (104/85): total (104/89)
Fisher's exact test strand distribution p-value = 4.36e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.82e-01

Only 100 of 229 total aligned reads displayed.


CGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTC  >  NC_014034/2249289‑2249485


cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                                       >  1:296729/1‑101 (MQ=255)


cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                                       <  1:4495603/101‑1 (MQ=255)


cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                                       >  2:2785752/1‑101 (MQ=255)


cgtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg                                                                                                       <  2:285407/101‑1 (MQ=255)


 gtGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGTAAATATCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAg.c                                                                                                     >  2:4617883/1‑101 (MQ=255)


   gCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAg.c.g.c                                                                                                 >  1:1325977/1‑101 (MQ=255)


    ccGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.cc                                                                                                >  1:3550859/1‑101 (MQ=255)


     ccattcaccccccGGGATATTTTCGGCAAGATGAA...AGCC..C..CCCTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCATAGGGGGGCAG.C.G.cc                                                                                                >  2:3809676/9‑101 (MQ=11)


       ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAg.c.g.c                                                                                                 <  1:527047/101‑1 (MQ=17)


         actccccccGGGATATTTGCAGCAAGATGAAACCGGAT..G..CCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGCGAA..GCCAGAGGGGGGCAG.C.G.ccc                                                                                               <  2:412404/97‑1 (MQ=17)


          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc.c.cctt                                                                                        <  1:3089351/101‑4 (MQ=255)


          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc.c.cctt                                                                                        >  1:358914/1‑98 (MQ=255)


          cT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTCCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc.c.cctt                                                                                        <  1:4020275/101‑4 (MQ=255)


           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc.c.ccttc                                                                                       <  2:3059914/101‑5 (MQ=255)


           t.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc.c.ccttc                                                                                       <  1:1557905/101‑5 (MQ=255)


             cccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc.c.ccttcg                                                                                      >  1:812051/1‑96 (MQ=255)


             cccccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.ccc.c.ccttcg                                                                                      <  2:677650/101‑6 (MQ=255)


               cccGGGATATTTGCGGCAAGATGAA...GGGGCAT..GATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCC.C.Ctgc                                                                                        <  2:4544891/101‑3 (MQ=0)


                ccGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCgcgg                                                                                    <  2:607886/101‑1 (MQ=255)


                 cGGGATATTTGCGGCAAGATGAA...GGGG..CATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAAAGGCCAAGAGGGGGCAG.C.G.CCC.C.Ctgccc                                                                                      >  2:3553439/1‑97 (MQ=2)


                  gggATATTTGGGGCAAGAGGAG...GGGG..G..GCTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.GGGCGGA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCgcgggc                                                                                  <  1:988755/101‑1 (MQ=25)


                  gggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCgcgggc                                                                                  >  2:487361/1‑101 (MQ=255)


                   ggATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCgcgggcg                                                                                 <  1:2643380/101‑1 (MQ=255)


                      tatTTGAGGCAAGAGGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGcc                                                                              <  1:3167913/101‑1 (MQ=255)


                       atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGccc                                                                             >  1:106802/1‑100 (MQ=255)


                       atTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCcg                                                                             >  2:1946907/1‑101 (MQ=255)


                        tttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCcgc                                                                            >  1:277983/1‑101 (MQ=255)


                         ttGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCcgcg                                                                           <  2:264081/101‑1 (MQ=255)


                          tGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGa                                                                          >  1:3362466/1‑101 (MQ=255)


                          tGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGa                                                                          >  1:170387/1‑101 (MQ=255)


                          tGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGCGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGa                                                                          >  1:1903821/1‑101 (MQ=255)


                           gAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGAc                                                                         <  1:1953156/101‑1 (MQ=255)


                               aaaGATGAG...GGGG..G..GTTTTCATCTTGCCGAAAATATCCGGGGGGGTGAA.TGGCGCA..GCCAGGGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGAcgcgc                                                                     <  1:4168614/100‑1 (MQ=25)


                                aaGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGAcgcgcg                                                                    >  2:3307546/1‑101 (MQ=255)


                                aaGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGAcgcgcg                                                                    >  2:1824702/1‑101 (MQ=255)


                                aaGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGAcgcgcg                                                                    >  1:2517704/1‑101 (MQ=255)


                                 aGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACgcgcgc                                                                   >  2:1297249/1‑101 (MQ=255)


                                  gATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGcgcc                                                                  <  1:4351254/101‑1 (MQ=255)


                                   aTGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGcgccg                                                                 <  2:1350217/101‑1 (MQ=255)


                                    tGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGccccac                                                                >  2:3969880/1‑99 (MQ=255)


                                       a...GGGG..G..TCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                             <  1:637770/101‑1 (MQ=255)


                                       a...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                             <  2:244620/101‑1 (MQ=255)


                                       a...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                             >  1:2704383/1‑101 (MQ=255)


                                       a...GGGG..G..GCTTTCATCTCGCAGAAAATAGCCGGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAAc                                                             <  1:230433/101‑1 (MQ=37)


                                             gg..g..gCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAgg                                                          >  2:4531718/1‑101 (MQ=255)


                                                       ttCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGc                                                    >  2:1795035/1‑101 (MQ=255)


                                                          aTCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGcccc                                                 >  2:3437060/1‑101 (MQ=255)


                                                            cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                               >  1:1769054/1‑101 (MQ=255)


                                                            cTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCtt                                               <  1:1135972/101‑1 (MQ=255)


                                                               gCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGt                                            >  2:230904/1‑101 (MQ=255)


                                                                cgaaaaaTATCGCGGGGGGTGAA.GGGCGCA..GCCAGGGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTc                                           <  2:1688591/98‑1 (MQ=38)


                                                                 cGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCa                                          >  1:3733537/1‑101 (MQ=255)


                                                                  gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAGCC.C.CCC.C.CTTCGCGGGGGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                         <  1:1468801/101‑1 (MQ=38)


                                                                  gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                         <  1:4294672/101‑1 (MQ=255)


                                                                  gAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAt                                         >  2:2895895/1‑101 (MQ=255)


                                                                   aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                        >  1:9936/1‑101 (MQ=255)


                                                                   aaaaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCAtt                                        <  1:3643399/101‑1 (MQ=255)


                                                                     aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                      <  1:2082068/101‑1 (MQ=255)


                                                                     aaTATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCt                                      >  2:4167126/1‑101 (MQ=255)


                                                                      atatCCCGGGGGGGGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTGGTGCCCCTTTGTCCTTCTg                                     >  1:422611/1‑101 (MQ=37)


                                                                         tCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCGTTGTCATTCTGttg                                  >  2:2566277/1‑99 (MQ=255)


                                                                          cgcGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                                 <  2:4243666/99‑1 (MQ=255)


                                                                          cccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGc                                 >  1:4316619/1‑101 (MQ=255)


                                                                           ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCa                                <  1:2215507/101‑1 (MQ=255)


                                                                           ccGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGCCATTCTGTCGCa                                >  1:3894795/1‑101 (MQ=255)


                                                                            cGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAc                               >  1:708522/1‑101 (MQ=255)


                                                                             ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCGGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                              >  2:2810997/1‑101 (MQ=255)


                                                                             ggggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAcc                              >  2:1074043/1‑101 (MQ=255)


                                                                              gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                             >  2:916540/1‑101 (MQ=255)


                                                                              gggggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCAccc                             >  2:4075529/1‑101 (MQ=255)


                                                                                gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                           >  1:2405869/1‑101 (MQ=255)


                                                                                gggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCAt                           <  2:2206508/101‑1 (MQ=255)


                                                                                 ggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                          >  1:3321197/1‑101 (MQ=255)


                                                                                 ggTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                          >  2:239263/1‑101 (MQ=255)


                                                                                 ggTGAA.TGGCGCA..GACGGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCCACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATg                          <  1:4618136/101‑1 (MQ=255)


                                                                                  gTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGt                         >  1:2161967/1‑101 (MQ=255)


                                                                                    gAA.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCa                       >  2:4012377/1‑101 (MQ=255)


                                                                                      a.TGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATa                     <  2:4355028/101‑1 (MQ=255)


                                                                                        tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                    >  2:1160350/1‑101 (MQ=255)


                                                                                        tGGCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATaa                    <  2:3635237/101‑1 (MQ=255)


                                                                                         ggCGCA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAg                   >  2:836456/1‑101 (MQ=255)


                                                                                           cgcG..GCCGGAGGGGGGCAGCC.C.CCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                 <  1:4136294/101‑1 (MQ=255)


                                                                                           cgcA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                 <  2:811978/101‑1 (MQ=255)


                                                                                           cgcA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCt                 >  1:1215679/1‑101 (MQ=255)


                                                                                             cA..GCGAGGGGGGGGCTC.CTG.CTC.C.GTTTGCGGGCAGCTGCGGCGCGCGTCGCTAAAGGTGGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg               <  2:1396445/101‑1 (MQ=255)


                                                                                             cA..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTgg               >  1:2769837/1‑101 (MQ=255)


                                                                                              a..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc              <  1:1737718/101‑1 (MQ=255)


                                                                                              a..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGc              >  2:2151677/1‑101 (MQ=255)


                                                                                              a..GCCAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTGTGTCATTCTGTCGCACCCATGTCATAAGCTGGc              >  1:4088317/1‑101 (MQ=255)


                                                                                                   cAGAGGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGc           >  1:2450390/1‑101 (MQ=255)


                                                                                                    agagGGGGGCAG.C.G.CCCGC.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGGCATAAGCTGGCCGCa          >  2:3863459/1‑101 (MQ=255)


                                                                                                     gagGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCAt         >  1:2146416/1‑101 (MQ=255)


                                                                                                      agGGGGGCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATc        >  2:1316180/1‑101 (MQ=255)


                                                                                                       ggggggCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTTATAAGCTGGCCGCATCg       <  2:366361/101‑1 (MQ=255)


                                                                                                        gggggCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGa      >  2:2348138/1‑101 (MQ=255)


                                                                                                          gggCAG.C.CCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATg    <  2:3872517/101‑1 (MQ=255)


                                                                                                           ggCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGt   >  1:739176/1‑101 (MQ=255)


                                                                                                            gCAG.C.GCCCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc  >  2:4247014/1‑101 (MQ=255)


                                                                                                            gCAG.C.GCCCC.C.CTTCGCGCGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc  <  2:3131036/101‑1 (MQ=255)


                                                                                                            gCAG.C.G.CCC.CGCTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTc  <  2:1986648/101‑1 (MQ=255)


CGTGCCGGGACT.CCCCCGGGATATTTGAGGCAAGATGAA...GGGG..G..GCTTTCATCTTGCCGAAAATATCCCGGGGGGTGAA.TGGCGCA..GCCAGAGGGGGGCAG.C.G.CCC.C.CTTCGCGGGCGGCCGCGACGCGCGCCGCAACAGGTCGTGCCCCTTTGTCATTCTGTCGCACCCATGTCATAAGCTGGCCGCATCGATGTC  >  NC_014034/2249289‑2249485

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 24 ≤


ATCG

< 33 ≤


ATCG

< 39 ≤


ATCG