Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,252,630 +G 100% coding (223/1050 nt) ltaE → threonine aldolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,252,6301.→G100.0% 705.2 187coding (223/1050 nt)ltaEthreonine aldolase
Reads supporting (aligned to +/- strand):  new base (79/106):  ref base (0/2):  total (79/108)

Only 100 of 206 total aligned reads displayed.

GCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.GGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAGCTGGTGCTGGTC  >  NC_014034/2252530‑2252725
                                                                                                     |                                                                                                
gCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCt                                                                                                   <  2:779922/101‑1 (MQ=255)
gCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCt                                                                                                   <  1:4012018/101‑1 (MQ=255)
gCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCt                                                                                                   <  1:1985379/101‑1 (MQ=255)
 cATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.g                                                                                                 <  2:2374685/101‑1 (MQ=255)
 cATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.g                                                                                                 <  1:4632532/101‑1 (MQ=255)
 cATCGGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.g                                                                                                 >  2:4017903/1‑101 (MQ=255)
  aTCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gg                                                                                                >  1:677548/1‑101 (MQ=255)
   tCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.ggg                                                                                               <  2:2143188/101‑1 (MQ=255)
   tCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.ggg                                                                                               <  1:3371638/101‑1 (MQ=255)
   tCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.ggg                                                                                               <  1:802492/101‑1 (MQ=255)
    cTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.gggg                                                                                              <  2:677484/101‑1 (MQ=255)
             aTTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGAttt                                                                                     >  1:4692474/1‑101 (MQ=255)
             aTTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGAttt                                                                                     <  1:3123582/101‑1 (MQ=255)
             aTTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGAttt                                                                                     >  2:1982763/1‑101 (MQ=255)
              ttttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGAtttt                                                                                    <  2:2970724/101‑1 (MQ=255)
              ttttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGAtttt                                                                                    <  2:4692218/101‑1 (MQ=255)
               tttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTc                                                                                   >  1:3194193/1‑101 (MQ=255)
                ttCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCt                                                                                  >  2:4629486/1‑101 (MQ=255)
                 tCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTg                                                                                 <  2:3752234/101‑1 (MQ=255)
                   gAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCTCGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTc                                                                               <  2:1881650/101‑1 (MQ=255)
                    aGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCa                                                                              <  2:4410798/101‑1 (MQ=255)
                     ggCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCAc                                                                             <  2:3833967/101‑1 (MQ=255)
                      gcgcCGCAGGCCGTTGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCAcc                                                                            <  2:1368487/101‑1 (MQ=255)
                       cgccgcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCg                                                                           >  1:3508739/1‑101 (MQ=255)
                       cgccgcAGGACGTGGTCTATCTGGGGGCGATGGGCAGGGCGGCGAATGCCTTCTCCTGTGTGGTGCTGACCCAGCCCT.GGGGGCGCGATTTTCTGGCACCg                                                                           >  2:564089/1‑101 (MQ=255)
                         ccgcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCa                                                                         <  1:2382711/101‑1 (MQ=255)
                           gcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAAc                                                                       >  1:2744262/1‑101 (MQ=255)
                           gcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAAc                                                                       <  1:476474/101‑1 (MQ=255)
                           gcAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAAc                                                                       <  1:2214607/101‑1 (MQ=255)
                            cAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACg                                                                      >  1:1785759/1‑101 (MQ=255)
                            cAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACg                                                                      >  2:3971570/1‑101 (MQ=255)
                             aGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGc                                                                     >  2:2645557/1‑101 (MQ=255)
                                 cGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCat                                                                 <  2:2805389/101‑1 (MQ=255)
                                 cGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCat                                                                 <  1:4510293/101‑1 (MQ=255)
                                  gTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCata                                                                >  2:4608362/1‑101 (MQ=255)
                                  gTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCata                                                                <  1:1555908/101‑1 (MQ=255)
                                  gTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCata                                                                >  2:1282134/1‑101 (MQ=255)
                                  gTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCata                                                                <  2:3307735/101‑1 (MQ=255)
                                   tGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCatat                                                               <  1:2139662/101‑1 (MQ=255)
                                   tGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCatat                                                               >  1:2965272/1‑101 (MQ=255)
                                   tGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCatat                                                               <  1:4290186/101‑1 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATc                                                              <  1:2582196/101‑1 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATc                                                              >  1:458624/1‑101 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATc                                                              >  1:4248912/1‑101 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATc                                                              <  2:2587864/101‑1 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATc                                                              <  1:3763148/101‑1 (MQ=255)
                                    ggTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATc                                                              >  1:2445445/1‑101 (MQ=255)
                                     gTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCg                                                             <  1:1457401/101‑1 (MQ=255)
                                     gTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCg                                                             <  1:922459/101‑1 (MQ=255)
                                     gTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCg                                                             >  2:4306066/1‑101 (MQ=255)
                                     gTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCg                                                             >  1:3347078/1‑101 (MQ=255)
                                        tATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAgg                                                          <  2:2797642/101‑1 (MQ=255)
                                        tATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAgg                                                          >  1:1346685/1‑101 (MQ=255)
                                        tATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAgg                                                          <  2:3491910/101‑1 (MQ=255)
                                        tATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAgg                                                          <  1:2454269/101‑1 (MQ=255)
                                        tATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCAACGCAACGCCCATATCGAgg                                                          >  1:4730836/1‑101 (MQ=255)
                                         aTCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGa                                                         <  1:1765184/101‑1 (MQ=255)
                                           cTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAg                                                       <  2:4067059/101‑1 (MQ=255)
                                             ggtggCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGAc                                                     <  2:2928516/101‑1 (MQ=255)
                                              gtggCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACg                                                    <  1:1931936/101‑1 (MQ=255)
                                               tggCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGa                                                   <  1:2396621/101‑1 (MQ=255)
                                                ggCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGaa                                                  <  2:2707981/101‑1 (MQ=255)
                                                ggCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCAAATCGAGGAAGACGaa                                                  <  1:4651112/101‑1 (MQ=255)
                                                 gCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAAt                                                 <  2:1788311/101‑1 (MQ=255)
                                                 gCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAAt                                                 <  1:2089736/101‑1 (MQ=255)
                                                  cGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATg                                                <  2:764115/101‑1 (MQ=255)
                                                    aCGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATgcg                                              >  1:3057995/1‑101 (MQ=255)
                                                     cGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATgcgg                                             >  2:3668374/1‑101 (MQ=255)
                                                     cGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATgcgg                                             <  1:2645711/101‑1 (MQ=255)
                                                        gCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGgcgc                                          >  2:196700/1‑101 (MQ=255)
                                                        gCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTAACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGgcgc                                          >  2:3631562/1‑101 (MQ=255)
                                                          aCGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGccc                                        >  1:1417480/1‑101 (MQ=255)
                                                               ggcgAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGaa                                   <  2:4112857/101‑1 (MQ=255)
                                                               ggcgAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGaa                                   <  1:4608615/101‑1 (MQ=255)
                                                                 cgAATCCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAAtt                                 <  1:595149/101‑1 (MQ=255)
                                                                  gAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCGCCGAATTc                                >  2:2097355/1‑101 (MQ=255)
                                                                  gAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTc                                >  2:1133138/1‑101 (MQ=255)
                                                                      gcgcTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTaca                            >  1:832055/1‑101 (MQ=255)
                                                                        gcTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATTTCGAGGAAAACGAATGCGGCGCCCCCGAGTTCTACAcc                          <  2:1591801/101‑1 (MQ=255)
                                                                        gcTCTCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACAcc                          <  2:3931356/101‑1 (MQ=255)
                                                                         ctctCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTacacag                         >  2:4225211/1‑99 (MQ=255)
                                                                         ctctCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACAcca                         <  1:1092008/101‑2 (MQ=255)
                                                                         ctctCCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACcg                         >  2:1221984/1‑101 (MQ=255)
                                                                           ctcCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACcggc                       <  2:3096612/101‑1 (MQ=255)
                                                                           ctcCTGTGCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACcggc                       <  2:1725814/101‑1 (MQ=255)
                                                                               tgtgCGGGGCTGGCCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCt                   <  1:1261981/101‑1 (MQ=255)
                                                                                gtgCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTc                  >  2:2034931/1‑101 (MQ=255)
                                                                                gtgCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTc                  >  1:1016322/1‑101 (MQ=255)
                                                                                  gCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCa                <  2:3346876/101‑1 (MQ=255)
                                                                                  gCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCa                <  2:2109392/101‑1 (MQ=255)
                                                                                  gCGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCa                <  2:1068581/101‑1 (MQ=255)
                                                                                   cGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCaa               >  1:4178914/1‑101 (MQ=255)
                                                                                   cGGTGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCaa               <  1:2905937/101‑1 (MQ=255)
                                                                                      tGCTGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgct            >  1:1142954/1‑101 (MQ=255)
                                                                                         tGACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggt         >  1:1531734/1‑101 (MQ=255)
                                                                                          gACCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggtg        <  2:3193992/101‑1 (MQ=255)
                                                                                           aCCCAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggtgc       >  1:3842173/1‑101 (MQ=255)
                                                                                             ccAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGGTCCAAgctggtgctg     >  2:427044/1‑101 (MQ=255)
                                                                                              cAGCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAgctggtgctgg    <  2:887304/101‑1 (MQ=255)
                                                                                                gCCCTGGGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAGCTGGTGCTGGTc  >  2:1155253/1‑101 (MQ=255)
                                                                                                     |                                                                                                
GCATCTGCGCGAGATTTTCGAGGCGCCGCAGGCCGTGGTCTATCTGGTGGCGACGGGCACGGCGGCGAATGCGCTCTCCTGTGCGGTGCTGACCCAGCCCT.GGGGG.GCGATTTTCTGTCACCGCAACGCCCATATCGAGGAAGACGAATGCGGCGCCCCCGAATTCTACACCGGCGGCTCCAAGCTGGTGCTGGTC  >  NC_014034/2252530‑2252725

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG