Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,888 +G 100% coding (2687/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,8881.→G100.0% 712.0 195coding (2687/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (82/113):  ref base (0/0):  total (82/113)

Only 100 of 222 total aligned reads displayed.

GCGGACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGG  >  NC_014034/2304788‑2304980
                                                                                                     |                                                                                              
 cggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.gg                                                                                              >  1:389841/1‑100 (MQ=255)
 cggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAAAGGCGGCGCCGGTGGCG.CCTGCATGC.gg                                                                                              >  2:3288256/1‑100 (MQ=255)
  ggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.gga                                                                                             >  2:2508117/1‑99 (MQ=255)
  ggACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGC.CCCTGCATGC.gga                                                                                             >  2:3216192/1‑99 (MQ=255)
  ggACAAGGGCAAGATCCGGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.gga                                                                                             <  1:4007363/101‑3 (MQ=255)
     cAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.ggaaga                                                                                          >  1:51395/1‑96 (MQ=255)
     cAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGC.ggaaga                                                                                          >  1:243159/1‑96 (MQ=255)
     cAAGGGAAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGGGGCG.CCTGCATGC.ggaaga                                                                                          >  2:2041763/1‑96 (MQ=255)
      aaGAGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAACCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGAc                                                                                          >  2:1224994/1‑101 (MQ=255)
       aGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGAcc                                                                                         <  2:604223/101‑1 (MQ=255)
       aGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGAcc                                                                                         >  1:2271166/1‑101 (MQ=255)
       aGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGAcc                                                                                         <  1:2250639/101‑1 (MQ=255)
       aGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGAcc                                                                                         >  1:2107227/1‑101 (MQ=255)
       aGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGAcc                                                                                         <  2:2411038/101‑1 (MQ=255)
        gggCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg                                                                                        >  1:364179/1‑101 (MQ=255)
         ggCAAGATCCTGATAATCTTGCGCAAACCGCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.c                                                                                      <  1:1031217/101‑1 (MQ=255)
         ggCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.c                                                                                      >  2:3640668/1‑101 (MQ=255)
          gCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.cc                                                                                     >  2:1297332/1‑100 (MQ=255)
           cAAGATCCTGATAATCTGGCGCAAACCTCGGCCGGATCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccg                                                                                    <  1:606181/101‑3 (MQ=255)
           cAAGACCCGGATAATCTCGCACAACTCGCGGCCGGATCCCGCCTGTCAGCCGGCCTGCCGCACCGGCGTCGCCGGTGGCG.CGTGCACGCGGAAGACcg.ccg                                                                                    <  2:1583622/101‑3 (MQ=255)
            aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                   >  1:260313/1‑98 (MQ=255)
            aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                   >  1:2987736/1‑98 (MQ=255)
            aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                   >  1:4627269/1‑98 (MQ=255)
              gATCCTGATAATTTTGCGAAAAACCCGACCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtca                                                                                 <  2:583889/101‑6 (MQ=255)
               aTCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGGAGACcg.ccgtccg                                                                                >  2:1146121/1‑95 (MQ=255)
                 ccTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccggcagcc                                                                              >  1:1832795/1‑93 (MQ=255)
                  cTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagctc                                                                             >  1:3139556/1‑92 (MQ=255)
                       aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                         >  2:2990231/1‑101 (MQ=255)
                       aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                         >  2:548538/1‑101 (MQ=255)
                       aaTCTGGCGAAAACCTCGGCCGGACCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                         <  2:414959/101‑1 (MQ=255)
                        aTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                       >  2:839238/1‑101 (MQ=255)
                        aTCTTGCGAAAACCCCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                       <  1:1117755/101‑1 (MQ=255)
                              ggaaaacCTGGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtctg                                                                 <  1:129266/98‑6 (MQ=255)
                              cccAAACCCCGGCCGGACCCGGCCTGCCAGCGGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtctg                                                                 <  1:3361003/99‑6 (MQ=255)
                                cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtctggg                                                               >  1:2328890/1‑94 (MQ=255)
                                cAAAACCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcg                                                                <  1:2184000/101‑1 (MQ=255)
                                  aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGGCGCAACGGCGGCGCCGGAGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGcc                                                              >  1:3923908/1‑101 (MQ=255)
                                  aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                              >  2:2798213/1‑101 (MQ=255)
                                  aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGcc                                                              >  2:1796369/1‑101 (MQ=255)
                                     cccGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                           <  2:3354484/99‑1 (MQ=255)
                                     cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                           <  2:3904245/101‑1 (MQ=255)
                                        ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                        >  1:4347962/1‑101 (MQ=255)
                                        ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                        >  2:4351993/1‑101 (MQ=255)
                                         gcgggtcccGGCCTGCCACCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccga                                                       <  2:4377304/94‑3 (MQ=255)
                                         gcgggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCCCCCGCAGGGGCTGCCCCTCGCCca                                                       >  1:1349569/4‑101 (MQ=255)
                                         gCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                       <  2:3768009/101‑1 (MQ=255)
                                          ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                      >  2:3028332/1‑101 (MQ=255)
                                          ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcag                                                      >  1:969792/1‑101 (MQ=255)
                                           cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                     >  1:61794/1‑101 (MQ=255)
                                            ggATCCGGCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagca                                                    <  2:1100244/101‑1 (MQ=255)
                                             gATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagcag                                                   >  1:4338946/1‑101 (MQ=255)
                                              aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGCCTGCGCCTCGCCCagcagc                                                  >  1:275829/1‑101 (MQ=255)
                                                ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                >  2:2637855/1‑101 (MQ=255)
                                                  ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                              <  2:2765952/101‑1 (MQ=255)
                                                  ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCCCGCCCACCAGCGCCt                                              >  1:3700192/1‑101 (MQ=255)
                                                   gCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                             <  2:4256086/101‑1 (MQ=255)
                                                   gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                             <  1:3044505/101‑1 (MQ=255)
                                                   gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                             <  2:4049237/101‑1 (MQ=255)
                                                   gCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                             <  2:1430971/101‑1 (MQ=255)
                                                    ccTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                            <  1:3402657/101‑1 (MQ=255)
                                                     gtGTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                           <  2:3059551/100‑1 (MQ=255)
                                                     atctCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                           <  1:1506379/98‑1 (MQ=255)
                                                      tGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCgg                                          >  2:1239621/1‑100 (MQ=255)
                                                      ctccAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGt                                          <  2:2909108/98‑1 (MQ=255)
                                                       ggcAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                         <  1:252563/99‑1 (MQ=255)
                                                       gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                         <  1:2056508/101‑1 (MQ=255)
                                                        tcggCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcg                                        <  1:4194855/98‑1 (MQ=255)
                                                         cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCgagat                                       >  2:1506278/1‑97 (MQ=255)
                                                         cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                       <  2:3677310/101‑1 (MQ=255)
                                                         cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                       <  2:3867401/101‑1 (MQ=255)
                                                            ccGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGc                                    >  2:4451288/1‑101 (MQ=255)
                                                             cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                   <  2:2328757/101‑1 (MQ=255)
                                                                ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                >  2:502590/1‑101 (MQ=255)
                                                                  tGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGtt                              <  1:3217775/101‑1 (MQ=255)
                                                                   gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                             >  1:2034726/1‑101 (MQ=255)
                                                                     cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                           <  1:827818/101‑1 (MQ=255)
                                                                        aaaggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                        <  1:3288458/98‑1 (MQ=255)
                                                                        aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                        <  1:1895573/101‑1 (MQ=255)
                                                                        aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                        <  1:980753/101‑1 (MQ=255)
                                                                         aCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCTTTTTCCTCg                       <  2:3139356/101‑1 (MQ=255)
                                                                          cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                      <  1:4451538/101‑1 (MQ=255)
                                                                          cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                      >  1:4527551/1‑101 (MQ=255)
                                                                          cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                      >  1:2625467/1‑101 (MQ=255)
                                                                          cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                      <  1:3028520/101‑1 (MQ=255)
                                                                           ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                     <  2:4721233/101‑1 (MQ=255)
                                                                            gcggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                    <  1:2161126/101‑1 (MQ=255)
                                                                              ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                  <  2:717586/101‑1 (MQ=255)
                                                                                 gcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc               <  2:3691178/101‑1 (MQ=25)
                                                                                     gTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGCCTGTGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGGGTTTTGCTCGAAAATCGCCGCa           >  2:3972497/1‑101 (MQ=255)
                                                                                      gtcgcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa           <  2:3625585/95‑1 (MQ=25)
                                                                                       tggccccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt          <  2:1012558/97‑1 (MQ=25)
                                                                                       gggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt          <  1:2147226/96‑1 (MQ=25)
                                                                                        ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt         <  1:3550532/97‑1 (MQ=25)
                                                                                          cgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt       >  2:3411482/3‑101 (MQ=21)
                                                                                            ccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc     >  2:2157026/1‑101 (MQ=25)
                                                                                             cTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt    >  1:1678615/1‑101 (MQ=25)
                                                                                             cTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt    >  2:3189979/1‑101 (MQ=25)
                                                                                             cTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt    >  2:2688921/1‑101 (MQ=21)
                                                                                              tGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg   <  1:1225084/101‑1 (MQ=25)
                                                                                               gCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTgg  >  2:4638122/1‑101 (MQ=21)
                                                                                                     |                                                                                              
GCGGACAAGGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGG  >  NC_014034/2304788‑2304980

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG