Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,896 +C 100% coding (2679/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,8961.→C100.0% 618.1 174coding (2679/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (69/105):  ref base (0/0):  total (69/105)

Only 100 of 211 total aligned reads displayed.

GGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCG.CA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304796‑2304988
                                                                                                      |                                                                                              
 ggCAAGATCCTGATAATCTTGCGCAAACCGCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.c                                                                                               <  1:1031217/101‑1 (MQ=255)
 ggCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.c                                                                                               >  2:3640668/1‑101 (MQ=255)
   cAAGATCCTGATAATCTGGCGCAAACCTCGGCCGGATCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccg                                                                                             <  1:606181/101‑3 (MQ=255)
    aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                            >  1:260313/1‑98 (MQ=255)
    aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                            >  1:4153318/1‑98 (MQ=255)
    aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                            >  1:4627269/1‑98 (MQ=255)
    aaGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgt                                                                                            >  2:325100/1‑98 (MQ=255)
     aGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtc                                                                                           >  1:4451745/1‑97 (MQ=255)
     aGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtc                                                                                           <  2:294989/101‑5 (MQ=255)
         ccTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccggcagcc                                                                                       >  1:1832795/1‑93 (MQ=255)
         ccTGATAATCTGGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACcg.ccgtcagct                                                                                       <  2:1261384/101‑9 (MQ=255)
             aTAATCTTGCGCAAACCGCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCg                                                                                    <  1:296586/101‑1 (MQ=255)
             aTAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCg                                                                                    <  1:669034/101‑1 (MQ=255)
             aTAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCg                                                                                    >  2:2111189/1‑101 (MQ=255)
              tAATCTTGCGCAAAGCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.c                                                                                  <  1:4361421/101‑1 (MQ=255)
              tAATCTTGCGCAAACCTCGGCCGGATCGGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.c                                                                                  <  2:4391306/101‑1 (MQ=255)
              tAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCCGCTccccc                                                                                   >  2:587001/1‑99 (MQ=255)
               aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGACGACCGCCGTCAGCTCCCG.Ca                                                                                 >  1:3059739/1‑101 (MQ=255)
               aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.Ca                                                                                 >  2:548538/1‑101 (MQ=255)
               aaTCTGGCGAAAACCTCGGCCGGACCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.Ca                                                                                 <  2:414959/101‑1 (MQ=255)
                aTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.g                                                                               >  2:839238/1‑101 (MQ=255)
                aTCTTGCGAAAACCCCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.g                                                                               <  1:1117755/101‑1 (MQ=255)
                  cTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.ggg                                                                             >  1:232302/1‑100 (MQ=255)
                  cTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.ggg                                                                             <  2:2813870/101‑2 (MQ=255)
                   ttGCGCAAACCCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.gggt                                                                            <  2:2792108/101‑3 (MQ=255)
                    tGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.gggtc                                                                           <  2:2758676/101‑4 (MQ=255)
                    tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.gggtc                                                                           >  1:391101/1‑98 (MQ=255)
                      ggaaaacCTGGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CA.gggtctg                                                                         <  1:129266/98‑6 (MQ=255)
                       gcAAAACTCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTgc                                                                         <  1:2347181/101‑1 (MQ=255)
                         aaaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCCG.GGTCTgcgc                                                                       >  1:2181569/1‑101 (MQ=255)
                           acccgggCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCt                                                                     <  1:3528845/96‑1 (MQ=255)
                           accccGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCt                                                                     <  2:4071915/97‑1 (MQ=255)
                           aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGGCTGCGCCt                                                                     >  2:2432564/1‑101 (MQ=255)
                             cccGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCg                                                                   <  2:3354484/99‑1 (MQ=255)
                             cTCGGCGGGCTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCg                                                                   <  2:1225591/101‑1 (MQ=255)
                             cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCg                                                                   <  2:3904245/101‑1 (MQ=255)
                                ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGccc                                                                >  2:4351993/1‑101 (MQ=255)
                                 gcgggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCCCCCG.CAGGGGCTGCCCCTCGCCca                                                               >  1:1349569/4‑101 (MQ=255)
                                 gCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCca                                                               <  2:3768009/101‑1 (MQ=255)
                                  ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcag                                                              >  1:969792/1‑101 (MQ=255)
                                  ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcag                                                              >  2:3028332/1‑101 (MQ=255)
                                   cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcagc                                                             >  1:61794/1‑101 (MQ=255)
                                   cGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCcagc                                                             <  1:3618154/101‑1 (MQ=255)
                                      aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGCCTGCGCCTCGCCCagcagc                                                          >  1:275829/1‑101 (MQ=255)
                                        ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGGCGGTGG.GGCCTGCATGCGGGAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCCGCAgcgc                                                        >  2:1635307/1‑101 (MQ=255)
                                        ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAgcgc                                                        >  2:1096547/1‑101 (MQ=255)
                                          ggCTTGTCCGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                      <  2:1349477/101‑1 (MQ=255)
                                           gCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  2:4256086/101‑1 (MQ=255)
                                           gCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                     <  2:1430971/101‑1 (MQ=255)
                                             gtGTCGGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                   <  2:3059551/100‑1 (MQ=255)
                                               gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 <  1:2041884/101‑1 (MQ=255)
                                               gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 >  1:3655413/1‑101 (MQ=255)
                                               gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 <  1:2056508/101‑1 (MQ=255)
                                               gTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTc                                                 >  1:1427743/1‑101 (MQ=255)
                                                 cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCgagat                                               >  2:1506278/1‑97 (MQ=255)
                                                 cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               <  2:3677310/101‑1 (MQ=255)
                                                 cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                               <  2:3867401/101‑1 (MQ=255)
                                                     cgcccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGCCCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                           <  1:1635407/98‑1 (MQ=255)
                                                        ccTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                        >  2:502590/1‑101 (MQ=255)
                                                           gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                     >  1:3389591/1‑101 (MQ=255)
                                                             cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGCCGCGGCCGCGTTTTc                                   <  1:4003637/101‑1 (MQ=255)
                                                               cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                 <  2:3141518/101‑1 (MQ=255)
                                                                aaaggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:3288458/98‑1 (MQ=255)
                                                                aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  2:583296/101‑1 (MQ=255)
                                                                aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:1895573/101‑1 (MQ=255)
                                                                aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                <  1:980753/101‑1 (MQ=255)
                                                                  cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                              >  1:2625467/1‑101 (MQ=255)
                                                                  cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                              <  1:3028520/101‑1 (MQ=255)
                                                                  cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                              <  1:2433034/101‑1 (MQ=255)
                                                                   ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                             <  2:4721233/101‑1 (MQ=255)
                                                                    gcggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAAc                            <  1:2161126/101‑1 (MQ=255)
                                                                      ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACac                          >  2:2205314/1‑100 (MQ=255)
                                                                      ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          <  2:717586/101‑1 (MQ=255)
                                                                      ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                          >  2:4698022/1‑101 (MQ=255)
                                                                        cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                        <  2:4483070/101‑1 (MQ=25)
                                                                         gcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                       <  2:3691178/101‑1 (MQ=25)
                                                                           cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  2:2033051/1‑101 (MQ=25)
                                                                           cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                     >  2:2541776/1‑101 (MQ=25)
                                                                             gTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                   >  1:4338949/1‑101 (MQ=25)
                                                                             gTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGCCTGTGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGGGTTTTGCTCGAAAATCGCCGCa                   >  2:3972497/1‑101 (MQ=255)
                                                                              gtcgcgccTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                   <  2:3625585/95‑1 (MQ=25)
                                                                               tggccccTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                  <  2:1012558/97‑1 (MQ=25)
                                                                               gggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAt                  <  1:2147226/96‑1 (MQ=25)
                                                                                ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  1:2226037/97‑1 (MQ=25)
                                                                                ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                 <  1:4220803/97‑1 (MQ=25)
                                                                                  cgccTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt               >  2:3411482/3‑101 (MQ=21)
                                                                                   gccTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg              <  1:325134/100‑1 (MQ=25)
                                                                                     cTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt            >  2:3189979/1‑101 (MQ=25)
                                                                                     cTGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt            <  2:1103876/101‑1 (MQ=25)
                                                                                      tGCATGCGGAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTg           <  1:1225084/101‑1 (MQ=25)
                                                                                         atgcggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt        >  1:3147684/6‑101 (MQ=21)
                                                                                          tgcggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       <  1:303614/97‑1 (MQ=21)
                                                                                          tgcggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg       <  1:3777362/97‑1 (MQ=25)
                                                                                           gcggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa      <  1:482200/98‑1 (MQ=25)
                                                                                           gcggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa      <  1:2990419/98‑1 (MQ=25)
                                                                                           gcggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa      <  1:3320665/98‑1 (MQ=25)
                                                                                           gcggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa      <  1:839312/98‑1 (MQ=25)
                                                                                            cggAAGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc     <  1:3641637/99‑1 (MQ=25)
                                                                                               aaGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  <  2:2641973/101‑1 (MQ=21)
                                                                                               aaGACCGCCGTCAGCTCCCG.CAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg  <  1:1687139/101‑1 (MQ=21)
                                                                                                      |                                                                                              
GGGCAAGATCCTGATAATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCG.CA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTG  >  NC_014034/2304796‑2304988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG