Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,304,910 +G 100% coding (2665/2808 nt) RCAP_rcc02139 ← methyl‑accepting chemotaxis protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,304,9101.→G100.0% 605.4 172coding (2665/2808 nt)RCAP_rcc02139methyl‑accepting chemotaxis protein
Reads supporting (aligned to +/- strand):  new base (65/106):  ref base (1/0):  total (66/106)

Only 100 of 199 total aligned reads displayed.

AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACC  >  NC_014034/2304811‑2305004
                                                                                                      |                                                                                              
aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGACGACCGCCGTCAGCTCCCGCa                                                                                                 >  1:3059739/1‑101 (MQ=255)
aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                                                 >  2:2990231/1‑101 (MQ=255)
aaTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                                                 >  2:548538/1‑101 (MQ=255)
aaTCTGGCGAAAACCTCGGCCGGACCCGGCCGGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCa                                                                                                 <  2:414959/101‑1 (MQ=255)
 aTCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.g                                                                                               >  2:839238/1‑101 (MQ=255)
   cTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.ggg                                                                                             >  2:3585371/1‑100 (MQ=255)
   cTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.ggg                                                                                             >  1:3867643/1‑100 (MQ=255)
    ttGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggg                                                                                            >  1:20611/1‑99 (MQ=255)
     tGCGCAAACCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtc                                                                                           <  2:2758676/101‑4 (MQ=255)
     tGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtc                                                                                           >  2:3147187/1‑98 (MQ=255)
        gcAAAACTCGGCCGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgc                                                                                         <  1:2347181/101‑1 (MQ=255)
         cAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCA.gggtctggg                                                                                       >  1:2328890/1‑94 (MQ=255)
         cAAAACCCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTgcg                                                                                        <  1:2184000/101‑1 (MQ=255)
           aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGGCGCAACGGCGGCGCCGGAGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGcc                                                                                      >  1:3923908/1‑101 (MQ=255)
           aaaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGcc                                                                                      >  2:2798213/1‑101 (MQ=255)
            accTCGGCCGGTTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCt                                                                                     <  1:1146207/99‑1 (MQ=255)
            aaCTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCt                                                                                     >  1:2531066/1‑101 (MQ=255)
              cccGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                   <  2:3354484/99‑1 (MQ=255)
              cTCGGCGGGCTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                   <  2:1225591/101‑1 (MQ=255)
              cTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCg                                                                                   <  2:3904245/101‑1 (MQ=255)
                 ggCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                >  2:4351993/1‑101 (MQ=255)
                 ggCCGGACCCGGCCTGCCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGccc                                                                                <  1:2264591/101‑1 (MQ=255)
                  gcgggATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCCCCCGCAGGGGCTGCCCCTCGCCca                                                                               >  1:1349569/4‑101 (MQ=255)
                  gCCGGATCCGGCCTGGCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCca                                                                               <  1:1787098/101‑1 (MQ=255)
                   ccGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCCCAGGGTCTGCGCCTCGccccg                                                                              >  1:2173211/1‑99 (MQ=255)
                    cGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                                             >  1:61794/1‑101 (MQ=255)
                    cGGACCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCcagc                                                                             <  1:3618154/101‑1 (MQ=255)
                       acccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCagcagc                                                                          <  2:1366230/99‑1 (MQ=255)
                       aTCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGCCTGCGCCTCGCCCagcagc                                                                          >  1:275829/1‑101 (MQ=255)
                         ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGGCGGTGG.GGCCTGCATGCGGGAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCCGCAgcgc                                                                        >  2:1635307/1‑101 (MQ=255)
                         ccGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAgcgc                                                                        >  2:1096547/1‑101 (MQ=255)
                           ggCTTGTCCGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCt                                                                      <  2:1349477/101‑1 (MQ=255)
                           ggCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCCCGCCCACCAGCGCCt                                                                      >  1:3700192/1‑101 (MQ=255)
                            gCCTGTCAGCCGCCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                                     <  2:4256086/101‑1 (MQ=255)
                            gCCTGCCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTg                                                                     <  2:4049237/101‑1 (MQ=255)
                             ccTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGc                                                                    <  1:3402657/101‑1 (MQ=255)
                              atctCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCg                                                                   <  1:1506379/98‑1 (MQ=255)
                                  cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                                               <  2:3677310/101‑1 (MQ=255)
                                  cAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTcgc                                                               <  2:3867401/101‑1 (MQ=255)
                                      cGGCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGcc                                                           <  2:2328757/101‑1 (MQ=255)
                                       ggCCTGCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcg                                                          >  1:204438/1‑101 (MQ=255)
                                         ccggCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGGCCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCcgcg                                                        <  2:3789532/98‑1 (MQ=255)
                                            gCCGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGttt                                                     >  1:2034726/1‑101 (MQ=255)
                                              cGCAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTc                                                   <  1:827818/101‑1 (MQ=255)
                                               gCAACGGCGGCGCCGGGGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTcc                                                  <  1:2151575/101‑1 (MQ=255)
                                                cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                                 >  2:4402880/1‑101 (MQ=255)
                                                cAACGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGGCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCt                                                 >  2:3632034/1‑101 (MQ=255)
                                                 aaaggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                <  1:3288458/98‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                <  1:1895573/101‑1 (MQ=255)
                                                 aaCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTc                                                <  1:980753/101‑1 (MQ=255)
                                                  aCGGCGGCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCTTTTTCCTCg                                               <  2:3139356/101‑1 (MQ=255)
                                                   cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                                              <  1:2433034/101‑1 (MQ=255)
                                                   cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                                              <  1:4451538/101‑1 (MQ=255)
                                                   cggcggCGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGa                                              <  1:3028520/101‑1 (MQ=255)
                                                    ggcggcGCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGaa                                             <  2:4721233/101‑1 (MQ=255)
                                                       ggcgCCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACAt                                          >  2:4698022/1‑101 (MQ=255)
                                                        gcgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATc                                         >  2:3215561/1‑101 (MQ=25)
                                                         cgcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcg                                        <  2:4483070/101‑1 (MQ=25)
                                                          gcCGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgc                                       <  2:3691178/101‑1 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                     >  2:1495501/1‑101 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                     >  2:2033051/1‑101 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                     >  2:2541776/1‑101 (MQ=25)
                                                            cGGTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATcgccg                                     >  2:3750174/1‑101 (MQ=25)
                                                              gTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCa                                   >  1:4338949/1‑101 (MQ=25)
                                                              gTGGCG.CCTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGCCTGTGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGGGTTTTGCTCGAAAATCGCCGCa                                   >  2:3972497/1‑101 (MQ=255)
                                                                 ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                 <  1:3550532/97‑1 (MQ=25)
                                                                 ggcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCAtt                                 <  1:4220803/97‑1 (MQ=25)
                                                                  gcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtg                                >  1:1226424/4‑101 (MQ=25)
                                                                  gcgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGAGTTTTCCTCGAACATCGCCGCATtg                                <  1:1362823/98‑1 (MQ=21)
                                                                   cgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                               <  2:673117/99‑1 (MQ=25)
                                                                   cgccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgt                               <  1:1056337/99‑1 (MQ=25)
                                                                    gccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATtgtg                              <  1:325134/100‑1 (MQ=25)
                                                                     ccTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGc                             >  2:2157026/1‑101 (MQ=25)
                                                                      cTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt                            >  1:1678615/1‑101 (MQ=25)
                                                                      cTGCATGCGGAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCt                            <  2:1103876/101‑1 (MQ=25)
                                                                          atgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGt                        >  1:3147684/6‑101 (MQ=21)
                                                                           tgcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTg                       <  1:303614/97‑1 (MQ=21)
                                                                            gcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa                      <  1:2990419/98‑1 (MQ=25)
                                                                            gcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa                      <  1:3320665/98‑1 (MQ=25)
                                                                            gcggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGa                      <  1:482200/98‑1 (MQ=25)
                                                                             cggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAc                     <  1:3641637/99‑1 (MQ=25)
                                                                              ggAAGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGAcc                    <  1:909991/100‑1 (MQ=21)
                                                                                aaGACCGCCGTCAGCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTg                  <  2:2641973/101‑1 (MQ=21)
                                                                                    ccgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCa              <  1:1096633/92‑1 (MQ=21)
                                                                                     cgccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCaa             <  1:3370225/93‑1 (MQ=21)
                                                                                      gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg            >  2:3049317/8‑101 (MQ=25)
                                                                                      gccgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAg            >  2:248978/8‑101 (MQ=25)
                                                                                        cgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCt          <  2:2768734/96‑1 (MQ=255)
                                                                                        cgtcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCt          <  1:2672572/96‑1 (MQ=255)
                                                                                          tcagCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATAGCCGCATTGTGCTGGGTGAACTGATCAAGCTgg        >  1:4084120/4‑101 (MQ=25)
                                                                                            agCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGtt      <  1:2798378/100‑1 (MQ=255)
                                                                                            agCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGtt      <  2:488501/100‑1 (MQ=255)
                                                                                            agCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTCTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGtt      <  2:4108054/100‑1 (MQ=255)
                                                                                             gCTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTc     <  1:3216393/101‑1 (MQ=255)
                                                                                              cTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGGTGGGTGACCTGATCAAGCTGGTTCa    <  1:3905844/101‑1 (MQ=255)
                                                                                              cTCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCa    >  1:541986/1‑101 (MQ=255)
                                                                                               tCCCGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAc   >  2:7902/1‑101 (MQ=255)
                                                                                                cccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAcc  >  2:510360/1‑101 (MQ=255)
                                                                                                cccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAcc  <  2:1008451/101‑1 (MQ=255)
                                                                                                cccGCAGGGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCAcc  >  2:284818/1‑101 (MQ=255)
                                                                                                      |                                                                                              
AATCTTGCGCAAAACTCGGCCGGATCCGGCCTGTCAGCCGGCCTGCCGCAACGGCGGCGCCGGTGGCGCCCTGCATGC.GAAGACCG.CGTACGCTCCCGCA.GGTCTGCGCCTCGCCCAGCAGCGCCTGCGTCGCGGCCGCGTTTTCCTCGAACATCGCCGCATTGTGCTGGGTGACCTGATCAAGCTGGTTCACC  >  NC_014034/2304811‑2305004

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG