Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 235,595 | +C | 97.2% | intergenic (+24/+187) | RCAP_rcc00200 → / ← RCAP_rcc00201 | MarR family transcriptional regulator/adenine‑specific DNA‑methyltransferase |
Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 235,595 | 2 | .→C | 97.2% | 4.8 0.0 ‑1.9 | 143 | intergenic (+24/+187) | RCAP_rcc00200/RCAP_rcc00201 | MarR family transcriptional regulator/adenine‑specific DNA‑methyltransferase |
Reads supporting (aligned to +/- strand): new base (3/136): ref base (0/4): total (3/140) | ||||||||||
Fisher's exact test strand distribution p-value = 1.00e+00 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.40e-02 |
Only 100 of 158 total aligned reads displayed.
GCGACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..CCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGC > NC_014034/235495‑235686
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gcgACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCg < 2:1073088/101‑1 (MQ=255)
gACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..cc < 2:1237855/101‑1 (MQ=255)
gACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..cc < 1:2730854/101‑1 (MQ=255)
tCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGAGGAACGGGGGGCTT..T..CCG..cccccc < 1:2204984/101‑3 (MQ=255)
cGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccg < 1:211493/101‑4 (MQ=255)
cGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccg < 1:1700008/101‑4 (MQ=255)
cGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccg < 2:3602146/101‑4 (MQ=255)
ggNNNCNACAAGGTCCGNGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccgc < 2:337591/101‑5 (MQ=255)
cgcCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccgcac < 1:1799073/101‑7 (MQ=255)
ccGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccgcaccc < 2:4037034/101‑9 (MQ=255)
cGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..CCCCCCGcacccc < 2:3078052/101‑1 (MQ=255)
aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc < 2:4668681/101‑1 (MQ=255)
aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc < 2:4537552/101‑1 (MQ=255)
aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc < 2:4748722/101‑1 (MQ=255)
aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc < 1:222359/101‑1 (MQ=255)
aCAAGGCCCGCGCCCCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc < 1:1318094/101‑1 (MQ=255)
cAAGGTCCGCGGCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCg < 2:731345/101‑1 (MQ=255)
aaGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCgg < 2:2934303/101‑1 (MQ=255)
ggTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGc < 2:4297120/101‑1 (MQ=255)
ggTCCGCGCCGCCTTGCACGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGCGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGc < 1:4224153/101‑1 (MQ=255)
tCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGGGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGt < 1:1877923/101‑1 (MQ=255)
tCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGt < 1:4301624/101‑1 (MQ=255)
tCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGt < 1:2871723/101‑1 (MQ=255)
gcgcCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAtt < 1:1302826/101‑1 (MQ=255)
cgccgcCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAttt < 1:1895829/101‑1 (MQ=255)
cgccgcCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAttt < 2:1153542/101‑1 (MQ=255)
cgccgcCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAttt < 1:3590926/101‑1 (MQ=255)
ccgccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGc < 1:1445912/101‑1 (MQ=255)
cgccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCa < 2:636772/101‑1 (MQ=255)
ccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCAcc < 1:3305936/101‑1 (MQ=255)
ccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCAcc < 1:2223674/101‑1 (MQ=255)
ttGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCTGGGCGTATTTTCACCaa < 2:3426453/101‑1 (MQ=38)
tGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAg < 2:259299/101‑1 (MQ=255)
tGCCCGTGCTGCGCGAATTGCGGCTGCGGCGCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAg < 1:590695/101‑1 (MQ=38)
ggccGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGa < 2:867847/99‑1 (MQ=38)
gCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGa < 1:1586015/101‑1 (MQ=255)
gCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGa < 1:4491983/101‑1 (MQ=255)
cccGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaa < 2:2489760/101‑1 (MQ=255)
ccGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaa < 2:3654455/101‑1 (MQ=255)
cGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaa < 1:382936/101‑1 (MQ=255)
cGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaa < 1:4248276/101‑1 (MQ=255)
gTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaaa < 1:3613703/101‑1 (MQ=255)
gTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaaa < 2:267685/101‑1 (MQ=255)
gTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaaa < 2:2723092/101‑1 (MQ=255)
tgctgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAg < 1:2926846/101‑1 (MQ=255)
ctgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCg < 1:4493174/101‑1 (MQ=255)
ctgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCGGAAGCCGCGGAACGGGGGGCTT..TCCCCC..CCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCg < 1:519544/101‑1 (MQ=38)
tgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGa < 1:2489664/101‑1 (MQ=255)
gcgcGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGaa < 1:1035985/101‑1 (MQ=255)
cgcgAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAAc < 2:1726568/101‑1 (MQ=255)
cgAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACgg < 2:2308280/101‑1 (MQ=255)
cgAATGGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAAGGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACgg < 1:3341484/101‑1 (MQ=38)
aaTTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGt < 1:1764377/101‑1 (MQ=255)
aaTTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGt < 2:833118/101‑1 (MQ=255)
aaTTGCGGCTGCGGCTACAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGt < 2:4266270/101‑1 (MQ=38)
tggggctgcggctCGAGGAATGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCGCCAAGAAAAAGCGAACGGGTttc < 2:1053468/98‑1 (MQ=37)
gcggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttct < 1:2579669/101‑1 (MQ=255)
cggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttctt < 2:230122/101‑1 (MQ=255)
ggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttcttc < 1:427452/101‑1 (MQ=38)
ggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttcttc < 2:4462324/101‑1 (MQ=255)
gctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTtcttct < 1:2962980/101‑1 (MQ=255)
gtgcggctCGAGGAAGCCTGAAGCGGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTcttctt < 2:1603584/100‑1 (MQ=37)
ctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTcttctt < 1:1205981/101‑1 (MQ=255)
ctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTcttctt < 1:3076042/101‑1 (MQ=255)
cggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTggg < 1:1067927/101‑1 (MQ=255)
ggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGa < 1:2656733/101‑1 (MQ=255)
ggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGa < 2:3854187/101‑1 (MQ=255)
gctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaa < 1:3757546/101‑1 (MQ=255)
gctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaa < 1:3706959/101‑1 (MQ=255)
ctggAGGAAAGCTTAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaa < 1:919036/98‑1 (MQ=37)
tCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaaa < 2:1544106/101‑1 (MQ=255)
tCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaaa < 2:3025363/101‑1 (MQ=255)
cGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaaaa < 2:4449287/101‑1 (MQ=255)
gAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACg < 2:2986353/101‑1 (MQ=255)
aGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGccc < 1:721778/101‑1 (MQ=255)
gCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGcccc < 2:4520386/101‑1 (MQ=255)
cTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCt < 2:3226264/101‑1 (MQ=255)
tGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCtt < 1:1231492/101‑1 (MQ=255)
gAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttt < 2:2400980/101‑1 (MQ=255)
aaacgcacagAACGGGGGGCTTCCC..CCC..CCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttt < 1:309506/92‑1 (MQ=25)
aaGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCtttt < 2:788005/101‑1 (MQ=255)
aaGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCtttt < 1:4012471/101‑1 (MQ=255)
aGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttttt < 1:4376200/101‑1 (MQ=38)
aGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttttt < 2:2384989/101‑1 (MQ=255)
gCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTc < 2:2565740/101‑1 (MQ=255)
ccGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCt < 2:4513227/101‑1 (MQ=255)
cgcgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCtt < 1:2310505/101‑1 (MQ=255)
gcgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTg < 1:940348/101‑1 (MQ=255)
cgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCTGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTgg < 1:4046850/101‑1 (MQ=39)
cgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTgg < 2:1798828/101‑1 (MQ=255)
aaCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTc < 2:3924755/101‑1 (MQ=255)
aaCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTc < 1:693163/101‑1 (MQ=255)
aCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCa < 2:4493132/101‑1 (MQ=255)
aCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCa < 1:1518346/101‑1 (MQ=255)
aCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCa < 1:3567282/101‑1 (MQ=255)
cGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCaa < 2:1329149/101‑1 (MQ=255)
cGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCaa < 1:2507546/101‑1 (MQ=255)
ggggCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAACGAACGGGGTTCTTCTTGGGAAAAAAACGCCCCTTTTTTTTGGCTCAAATa > 1:4733632/1‑101 (MQ=37)
gCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGc > 2:2023846/1‑101 (MQ=255)
gCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGc < 1:2836941/101‑1 (MQ=255)
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GCGACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..CCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGC > NC_014034/235495‑235686
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 20 ≤
ATCG
< 27 ≤
ATCG
< 33 ≤
ATCG
< 40 ≤
ATCG
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