Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 235,595 +C 97.2% intergenic (+24/+187) RCAP_rcc00200 → / ← RCAP_rcc00201 MarR family transcriptional regulator/adenine‑specific DNA‑methyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034235,5952.→C97.2% 4.8 0.0 ‑1.9143intergenic (+24/+187)RCAP_rcc00200/RCAP_rcc00201MarR family transcriptional regulator/adenine‑specific DNA‑methyltransferase
Reads supporting (aligned to +/- strand):  new base (3/136):  ref base (0/4):  total (3/140)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.40e-02

Only 100 of 158 total aligned reads displayed.

GCGACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..CCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGC  >  NC_014034/235495‑235686
                                                                                                         |                                                                                            
gcgACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCg                                                                                               <  2:1073088/101‑1 (MQ=255)
  gACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..cc                                                                                           <  2:1237855/101‑1 (MQ=255)
  gACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..cc                                                                                           <  1:2730854/101‑1 (MQ=255)
      tCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGAGGAACGGGGGGCTT..T..CCG..cccccc                                                                                       <  1:2204984/101‑3 (MQ=255)
       cGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccg                                                                                      <  1:211493/101‑4 (MQ=255)
       cGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccg                                                                                      <  1:1700008/101‑4 (MQ=255)
       cGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccg                                                                                      <  2:3602146/101‑4 (MQ=255)
        ggNNNCNACAAGGTCCGNGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccgc                                                                                     <  2:337591/101‑5 (MQ=255)
          cgcCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccgcac                                                                                   <  1:1799073/101‑7 (MQ=255)
            ccGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..ccccccgcaccc                                                                                 <  2:4037034/101‑9 (MQ=255)
             cGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..CCCCCCGcacccc                                                                                <  2:3078052/101‑1 (MQ=255)
               aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc                                                                                <  2:4668681/101‑1 (MQ=255)
               aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc                                                                                <  2:4537552/101‑1 (MQ=255)
               aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc                                                                                <  2:4748722/101‑1 (MQ=255)
               aCAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc                                                                                <  1:222359/101‑1 (MQ=255)
               aCAAGGCCCGCGCCCCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCAcccccc                                                                                <  1:1318094/101‑1 (MQ=255)
                cAAGGTCCGCGGCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCg                                                                               <  2:731345/101‑1 (MQ=255)
                 aaGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCgg                                                                              <  2:2934303/101‑1 (MQ=255)
                   ggTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGc                                                                            <  2:4297120/101‑1 (MQ=255)
                   ggTCCGCGCCGCCTTGCACGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGCGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGc                                                                            <  1:4224153/101‑1 (MQ=255)
                     tCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGGGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGt                                                                          <  1:1877923/101‑1 (MQ=255)
                     tCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGt                                                                          <  1:4301624/101‑1 (MQ=255)
                     tCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGt                                                                          <  1:2871723/101‑1 (MQ=255)
                        gcgcCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAtt                                                                       <  1:1302826/101‑1 (MQ=255)
                         cgccgcCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAttt                                                                      <  1:1895829/101‑1 (MQ=255)
                         cgccgcCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAttt                                                                      <  2:1153542/101‑1 (MQ=255)
                         cgccgcCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTAttt                                                                      <  1:3590926/101‑1 (MQ=255)
                           ccgccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGc                                                                    <  1:1445912/101‑1 (MQ=255)
                            cgccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCa                                                                   <  2:636772/101‑1 (MQ=255)
                              ccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCAcc                                                                 <  1:3305936/101‑1 (MQ=255)
                              ccTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCAcc                                                                 <  1:2223674/101‑1 (MQ=255)
                                ttGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCTGGGCGTATTTTCACCaa                                                               <  2:3426453/101‑1 (MQ=38)
                                 tGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAg                                                              <  2:259299/101‑1 (MQ=255)
                                 tGCCCGTGCTGCGCGAATTGCGGCTGCGGCGCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAg                                                              <  1:590695/101‑1 (MQ=38)
                                  ggccGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGa                                                             <  2:867847/99‑1 (MQ=38)
                                  gCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGa                                                             <  1:1586015/101‑1 (MQ=255)
                                  gCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGa                                                             <  1:4491983/101‑1 (MQ=255)
                                   cccGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaa                                                            <  2:2489760/101‑1 (MQ=255)
                                    ccGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaa                                                           <  2:3654455/101‑1 (MQ=255)
                                     cGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaa                                                          <  1:382936/101‑1 (MQ=255)
                                     cGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaa                                                          <  1:4248276/101‑1 (MQ=255)
                                      gTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaaa                                                         <  1:3613703/101‑1 (MQ=255)
                                      gTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaaa                                                         <  2:267685/101‑1 (MQ=255)
                                      gTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGaaaaa                                                         <  2:2723092/101‑1 (MQ=255)
                                       tgctgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAg                                                        <  1:2926846/101‑1 (MQ=255)
                                         ctgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCg                                                      <  1:4493174/101‑1 (MQ=255)
                                         ctgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCGGAAGCCGCGGAACGGGGGGCTT..TCCCCC..CCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCg                                                      <  1:519544/101‑1 (MQ=38)
                                          tgcGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGa                                                     <  1:2489664/101‑1 (MQ=255)
                                           gcgcGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGaa                                                    <  1:1035985/101‑1 (MQ=255)
                                            cgcgAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAAc                                                   <  2:1726568/101‑1 (MQ=255)
                                              cgAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACgg                                                 <  2:2308280/101‑1 (MQ=255)
                                              cgAATGGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAAGGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACgg                                                 <  1:3341484/101‑1 (MQ=38)
                                                aaTTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGt                                               <  1:1764377/101‑1 (MQ=255)
                                                aaTTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGt                                               <  2:833118/101‑1 (MQ=255)
                                                aaTTGCGGCTGCGGCTACAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGt                                               <  2:4266270/101‑1 (MQ=38)
                                                   tggggctgcggctCGAGGAATGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCGCCAAGAAAAAGCGAACGGGTttc                                            <  2:1053468/98‑1 (MQ=37)
                                                    gcggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttct                                           <  1:2579669/101‑1 (MQ=255)
                                                     cggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttctt                                          <  2:230122/101‑1 (MQ=255)
                                                      ggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttcttc                                         <  1:427452/101‑1 (MQ=38)
                                                      ggctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTttcttc                                         <  2:4462324/101‑1 (MQ=255)
                                                       gctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTtcttct                                        <  1:2962980/101‑1 (MQ=255)
                                                        gtgcggctCGAGGAAGCCTGAAGCGGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTcttctt                                       <  2:1603584/100‑1 (MQ=37)
                                                        ctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTcttctt                                       <  1:1205981/101‑1 (MQ=255)
                                                        ctgcggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTcttctt                                       <  1:3076042/101‑1 (MQ=255)
                                                           cggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTggg                                    <  1:1067927/101‑1 (MQ=255)
                                                            ggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGa                                   <  1:2656733/101‑1 (MQ=255)
                                                            ggctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGa                                   <  2:3854187/101‑1 (MQ=255)
                                                             gctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaa                                  <  1:3757546/101‑1 (MQ=255)
                                                             gctCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaa                                  <  1:3706959/101‑1 (MQ=255)
                                                              ctggAGGAAAGCTTAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaa                                 <  1:919036/98‑1 (MQ=37)
                                                               tCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaaa                                <  2:1544106/101‑1 (MQ=255)
                                                               tCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaaa                                <  2:3025363/101‑1 (MQ=255)
                                                                cGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGaaaaa                               <  2:4449287/101‑1 (MQ=255)
                                                                    gAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACg                           <  2:2986353/101‑1 (MQ=255)
                                                                       aGCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGccc                        <  1:721778/101‑1 (MQ=255)
                                                                        gCTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGcccc                       <  2:4520386/101‑1 (MQ=255)
                                                                         cTGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCt                      <  2:3226264/101‑1 (MQ=255)
                                                                          tGAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCtt                     <  1:1231492/101‑1 (MQ=255)
                                                                           gAAGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttt                    <  2:2400980/101‑1 (MQ=255)
                                                                           aaacgcacagAACGGGGGGCTTCCC..CCC..CCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttt                    <  1:309506/92‑1 (MQ=25)
                                                                            aaGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCtttt                   <  2:788005/101‑1 (MQ=255)
                                                                            aaGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCtttt                   <  1:4012471/101‑1 (MQ=255)
                                                                             aGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCCCCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttttt                  <  1:4376200/101‑1 (MQ=38)
                                                                             aGCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCttttt                  <  2:2384989/101‑1 (MQ=255)
                                                                              gCCGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTc                 <  2:2565740/101‑1 (MQ=255)
                                                                               ccGCGGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCt                <  2:4513227/101‑1 (MQ=255)
                                                                                cgcgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCtt               <  1:2310505/101‑1 (MQ=255)
                                                                                 gcgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTg              <  1:940348/101‑1 (MQ=255)
                                                                                  cgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCTGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTgg             <  1:4046850/101‑1 (MQ=39)
                                                                                  cgGAACGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTgg             <  2:1798828/101‑1 (MQ=255)
                                                                                     aaCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTc          <  2:3924755/101‑1 (MQ=255)
                                                                                     aaCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTc          <  1:693163/101‑1 (MQ=255)
                                                                                      aCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCa         <  2:4493132/101‑1 (MQ=255)
                                                                                      aCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCa         <  1:1518346/101‑1 (MQ=255)
                                                                                      aCGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCa         <  1:3567282/101‑1 (MQ=255)
                                                                                       cGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCaa        <  2:1329149/101‑1 (MQ=255)
                                                                                       cGGGGGGCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCaa        <  1:2507546/101‑1 (MQ=255)
                                                                                          ggggCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAACGAACGGGGTTCTTCTTGGGAAAAAAACGCCCCTTTTTTTTGGCTCAAATa     >  1:4733632/1‑101 (MQ=37)
                                                                                             gCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGc  >  2:2023846/1‑101 (MQ=255)
                                                                                             gCTT..T..CCGCCCCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGc  <  1:2836941/101‑1 (MQ=255)
                                                                                                         |                                                                                            
GCGACATCGGCGCCGACAAGGTCCGCGCCGCCTTGCCCGTGCTGCGCGAATTGCGGCTGCGGCTCGAGGAAAGCTGAAGCCGCGGAACGGGGGGCTT..T..CCG..CCCCGCACCCCCCGGGCGTATTTGCACCAAGAAAAAGCGAACGGGTTTCTTCTTGGGAAAAATACGCCCCTTTTTCTTGGCTCAAATACGC  >  NC_014034/235495‑235686

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG