BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,486,428	+G	98.2%	intergenic (‑56/+138)	RCAP_rcc02312 ← / ← soxG	hypothetical protein/sarcosine oxidase subunit gamma

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,486,428	1	.→G	98.2%	2.8 ‑1.1 ‑1.9	223	intergenic (‑56/+138)	RCAP_rcc02312/soxG	hypothetical protein/sarcosine oxidase subunit gamma
Reads supporting (aligned to +/- strand): new base (100/118): ref base (3/1): total (103/120)
Fisher's exact test strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.31e-02

Only 100 of 252 total aligned reads displayed.


ATTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAA.GGG.G.CGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGG  >  NC_014034/2486328‑2486525


aTTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGa.aaa                                                                                                       <  2:2532836/101‑1 (MQ=255)


 ttGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAA.g                                                                                                     >  2:3017338/1‑101 (MQ=255)


  tGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAA.gg                                                                                                    >  2:1505193/1‑101 (MQ=255)


         gTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAA.ggg.ggcgct                                                                                            <  2:234752/101‑6 (MQ=255)


          tCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTg                                                                                           <  2:599097/101‑1 (MQ=255)


            gggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGcc                                                                                         >  1:2275030/1‑101 (MQ=255)


             ggCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGccc                                                                                        <  2:2431525/101‑1 (MQ=255)


              gCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGcccc                                                                                       <  1:3822340/101‑1 (MQ=255)


                cgcgcgCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGccccct                                                                                     >  2:3207310/1‑99 (MQ=255)


                 gcgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGccccctc                                                                                    <  2:377157/101‑4 (MQ=255)


                  cgcgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAA.GGG.GACGCTGccccctct                                                                                   >  1:4514921/1‑97 (MQ=255)


                   ccgcCCCCGGATTTCGAATAGTCCATTCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCtt                                                                                 <  1:2856697/100‑1 (MQ=255)


                    cgcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTtg                                                                                >  2:2504734/1‑101 (MQ=255)


                     gcCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTtg.c                                                                              <  1:212030/101‑1 (MQ=255)


                        cccGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTtg.ccct                                                                           >  1:1302685/1‑99 (MQ=255)


                           ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTtg.ccctgcc                                                                        >  2:3868257/1‑96 (MQ=255)


                           ggATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTtg.ccctgcc                                                                        >  1:3139182/1‑96 (MQ=255)


                            gATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCg                                                                        >  1:1199452/1‑101 (MQ=255)


                             aTTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCgg                                                                       <  1:4694742/101‑1 (MQ=255)


                                tCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGATGCCCC.CTCTTGCCCTGCCGGGCa                                                                    >  1:4565177/1‑101 (MQ=255)


                                 cGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCaa                                                                   <  2:2227753/101‑1 (MQ=38)


                                  gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                  >  1:4023474/1‑101 (MQ=38)


                                  gAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAg                                                                  >  2:1120553/1‑101 (MQ=38)


                                   aaTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGt                                                                 >  2:3563733/1‑101 (MQ=38)


                                    aTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTc                                                                <  2:1728938/101‑1 (MQ=38)


                                    aTAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTc                                                                >  1:1214823/1‑101 (MQ=38)


                                     tAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCa                                                               >  2:1077216/1‑101 (MQ=38)


                                        tCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccc                                                            >  1:2465142/1‑101 (MQ=37)


                                        tCCATGCGGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccc                                                            <  2:2158565/101‑1 (MQ=37)


                                         ccATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAcccc                                                           >  1:386737/1‑101 (MQ=37)


                                         ccATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAcccc                                                           <  1:3209481/101‑1 (MQ=37)


                                          cATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCAccccc                                                          <  2:4433624/101‑1 (MQ=37)


                                           aTGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCg                                                         <  1:787342/101‑1 (MQ=37)


                                            tGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCgg                                                        >  2:3066561/1‑101 (MQ=37)


                                            tGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCgg                                                        >  2:1996/1‑101 (MQ=37)


                                             gCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCggg                                                       >  2:1583364/1‑101 (MQ=37)


                                             gCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCggg                                                       <  2:4593379/101‑1 (MQ=37)


                                             gCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCggg                                                       >  2:2019341/1‑101 (MQ=37)


                                               tGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGat                                                     <  1:1869106/101‑1 (MQ=37)


                                                ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                    >  1:1238550/1‑101 (MQ=37)


                                                ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                    >  1:4174726/1‑101 (MQ=37)


                                                ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                    <  1:336308/101‑1 (MQ=37)


                                                ggATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGata                                                    >  1:2627044/1‑101 (MQ=37)


                                                 gATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGatat                                                   <  2:1302593/101‑1 (MQ=37)


                                                  aTCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATAtt                                                  <  1:4256551/101‑1 (MQ=37)


                                                   tctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTc                                                 <  1:4648585/101‑1 (MQ=37)


                                                   tctcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTc                                                 >  1:1691976/1‑101 (MQ=37)


                                                     tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCAt                                               >  1:2760288/1‑101 (MQ=37)


                                                     tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCAt                                               <  1:4195121/101‑1 (MQ=37)


                                                     tcCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCCGGAAATTCAt                                               >  1:2949022/1‑101 (MQ=25)


                                                     tcCTTTGCCTACTATCTACGTCTAGCCCGACCGCAAGGCAAGCGACAAC.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGTCAATTCTCCCCCGGGATATTCAt                                               <  2:780071/101‑1 (MQ=12)


                                                      ccTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATg                                              <  1:3017526/101‑1 (MQ=37)


                                                       cTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATgg                                             <  2:4302314/101‑1 (MQ=37)


                                                        tttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGc                                            >  2:4242302/1‑101 (MQ=37)


                                                         ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                           >  1:4747154/1‑101 (MQ=37)


                                                         ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                           <  2:3721046/101‑1 (MQ=37)


                                                         ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                           >  2:3168230/1‑101 (MQ=37)


                                                         ttGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCa                                           >  2:2893725/1‑101 (MQ=37)


                                                          tGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCaa                                          <  2:337088/101‑1 (MQ=37)


                                                          tGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCaa                                          >  1:251148/1‑101 (MQ=37)


                                                          tGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCaa                                          <  2:1018841/101‑1 (MQ=37)


                                                           gCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAg                                         >  1:1912524/1‑101 (MQ=37)


                                                           gCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAg                                         <  2:2626892/101‑1 (MQ=37)


                                                            ccTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGa                                        <  1:1001390/101‑1 (MQ=37)


                                                            ccTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGa                                        <  1:3083533/101‑1 (MQ=37)


                                                            ccTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGa                                        >  1:3376480/1‑101 (MQ=37)


                                                             cTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGAt                                       <  1:1505294/101‑1 (MQ=37)


                                                              ttCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATg                                      >  1:61273/1‑101 (MQ=37)


                                                               tCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGa                                     >  1:2595187/1‑101 (MQ=37)


                                                                cGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGaa                                    >  1:1549267/1‑101 (MQ=37)


                                                                cGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGaa                                    <  2:1184443/101‑1 (MQ=37)


                                                                cGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGaa                                    >  1:3582112/1‑101 (MQ=37)


                                                                 gATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAag                                   >  2:3408852/1‑101 (MQ=37)


                                                                  aTCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAagg                                  <  1:2086193/101‑1 (MQ=37)


                                                                       cGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCg                             <  2:4350986/101‑1 (MQ=37)


                                                                       cGGCAAGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCg                             >  2:121227/1‑101 (MQ=25)


                                                                           aaGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGtt                         >  2:1800554/1‑101 (MQ=25)


                                                                           aaGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CCCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGTTGAAGGAGGCgtgat                         >  1:1562235/1‑99 (MQ=25)


                                                                            aGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                        <  1:4134911/101‑1 (MQ=37)


                                                                            aGCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGttt                        >  2:4673676/1‑101 (MQ=25)


                                                                             gCCCGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTc                       >  1:3694910/1‑101 (MQ=25)


                                                                               ccGGCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTAAt                     >  2:4090678/1‑101 (MQ=25)


                                                                                 ggCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCt                   >  1:927040/1‑101 (MQ=25)


                                                                                  gCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCtt                  <  2:1826899/101‑1 (MQ=25)


                                                                                  gCCGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCtt                  <  2:4519980/101‑1 (MQ=25)


                                                                                   ccGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTg                 >  2:1059887/1‑101 (MQ=25)


                                                                                   ccGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATAGCAAGATGAAGGAGGCGTGTTTCATCTTg                 >  2:690125/1‑101 (MQ=25)


                                                                                    cGCAAGGCAAGCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGc                <  2:3451946/101‑1 (MQ=25)


                                                                                        aggcaagCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATa            <  1:1460852/101‑1 (MQ=21)


                                                                                        aggcaagCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATa            <  1:4495301/101‑1 (MQ=25)


                                                                                             agCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAatat       <  1:1038650/101‑1 (MQ=21)


                                                                                              gCGA.AAAGGGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATc      <  1:223038/101‑1 (MQ=21)


                                                                                              cgaa.aag.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATcc     <  2:3927915/98‑1 (MQ=21)


                                                                                              cgaa.aag.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATcc     >  2:3955092/4‑101 (MQ=21)


                                                                                              cgaa.aag.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATcc     <  1:13913/98‑1 (MQ=21)


                                                                                              cgaa.aag.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATcc     <  2:1372249/98‑1 (MQ=21)


                                                                                               gaa.aag.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATACccc    <  2:4023232/99‑1 (MQ=21)


                                                                                                gA.AAA.GGGTG.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGGCACCCCCGGGATATTCATGGCAAGATGAAGGAGCCGTGTTTCATCTTGCCATAAATATtcc    >  2:1698718/1‑101 (MQ=21)


                                                                                                aa.aag.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAGGGAGGCGTGTTTCATCTTGGCATAAATATCCCg   >  1:4074332/2‑101 (MQ=18)


                                                                                                 a.aag.GGG.G.CGCTGCCCC.CTCTTGCCCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCgg  >  2:2958451/1‑101 (MQ=21)


ATTGTTCTTGTCGGGCCGCGCGCCCCCGGATTTCGAATAGTCCATGCTGGATCTCCTTTGCCTTCGATCTGCGGCAAGCCCGGCCGCAAGGCAAGCGA.AAA.GGG.G.CGCTGCCCCTCTCTTG.CCTGCCGGGCAAGTCACCCCCGGGATATTCATGGCAAGATGAAGGAGGCGTGTTTCATCTTGCCATAAATATCCCGG  >  NC_014034/2486328‑2486525

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG