Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,060 +C 6.3% intergenic (+108/‑134) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0601.→C 6.3% 25.5 ‑1.1 ‑1.6158intergenic (+108/‑134)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/3):  ref base (72/76):  total (79/79)
Fisher's exact test strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.58e-02

Only 100 of 168 total aligned reads displayed.

CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCA.AGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493960‑2494152
                                                                                                      |                                                                                                   
cgcTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTAAA.TGGc                                                                                                      >  1:789771/1‑101 (MQ=255)
  cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                                 >  2:4291802/1‑101 (MQ=255)
  cTGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.a                                                                                                 <  1:3999310/101‑1 (MQ=255)
   tGCCCCCCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aa                                                                                                >  2:98397/1‑100 (MQ=255)
     cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                             <  1:1381414/101‑5 (MQ=255)
     cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                             >  2:3871363/1‑97 (MQ=255)
     cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagcc                                                                                             <  1:480788/101‑5 (MQ=255)
     cccccG.TCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.T.CAg                                                                                           <  1:2790042/101‑1 (MQ=255)
     cccccCGTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagc                                                                                              <  1:3577036/101‑4 (MQ=255)
         ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                          <  1:2848002/98‑8 (MQ=255)
         ccgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccaga                                                                                          >  2:617701/4‑94 (MQ=255)
          cgtCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.aagccagag                                                                                         <  2:2067283/99‑9 (MQ=255)
                   gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagc                                                                              >  1:2176995/1‑99 (MQ=18)
                   gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCaga                                                                              >  1:4734439/1‑99 (MQ=18)
                     ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAC.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                            >  1:4638215/1‑97 (MQ=17)
                      gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.cc                                                                        >  1:2725543/1‑101 (MQ=17)
                       aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.ccc                                                                       >  2:2414556/1‑101 (MQ=18)
                       aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.ccc                                                                       >  1:283443/1‑101 (MQ=18)
                        cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCG.cccc                                                                      <  1:402027/101‑1 (MQ=17)
                        cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCG.cccc                                                                      <  2:2954777/101‑1 (MQ=17)
                         tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.ccccc                                                                     >  1:806307/1‑101 (MQ=17)
                         tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.ccccc                                                                     >  2:128706/1‑101 (MQ=17)
                         tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.ccccc                                                                     >  1:1565968/1‑101 (MQ=18)
                           cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.cccccct                                                                   <  1:4471390/101‑3 (MQ=17)
                             cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGACCCCCTGc                                                                  >  2:1978451/1‑101 (MQ=17)
                              ccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCG.cccccctgcc                                                                <  2:4018385/101‑6 (MQ=17)
                               cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAT.AGGGGGGC..AGCGCCCCCCTGccc                                                                >  1:2925682/1‑101 (MQ=18)
                               cGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGccc                                                                <  1:2203745/101‑1 (MQ=17)
                                 ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGC.AGCG.CCCCCTGCCCcg                                                              <  2:1362966/101‑1 (MQ=0)
                                 ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGC.AGCG.CCCCCTGCCCcg                                                              <  1:4015443/101‑1 (MQ=0)
                                 ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCcg                                                              <  1:4417932/101‑1 (MQ=17)
                                   atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCcggg                                                            >  1:3337824/1‑100 (MQ=17)
                                    tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGC.AGCG.CCCCCTGCCCcgccg                                                           >  2:1139753/1‑101 (MQ=2)
                                    tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCcggcg                                                           >  1:1636751/1‑101 (MQ=18)
                                     atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCcggcgg                                                          >  2:1445592/1‑101 (MQ=18)
                                        tGCAGCAATATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTcc                                                       >  1:3303403/1‑101 (MQ=17)
                                        tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTcc                                                       >  2:263673/1‑101 (MQ=17)
                                        tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTcc                                                       <  1:3190927/101‑1 (MQ=17)
                                          cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGt                                                     <  1:4346247/101‑1 (MQ=17)
                                           agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                    <  1:998655/101‑1 (MQ=17)
                                           agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                    <  1:2414691/101‑1 (MQ=17)
                                             caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                                  <  1:2524071/101‑1 (MQ=17)
                                             caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGG.A.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                                  >  1:345412/1‑101 (MQ=18)
                                                gATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTggcggc                                               >  2:4232837/1‑101 (MQ=17)
                                                 aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg                                              >  2:1882578/1‑101 (MQ=18)
                                                   gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                            <  2:4421536/101‑1 (MQ=17)
                                                   gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGG.A.AGCGCCCCCCTGCCCCGGCGGTCCGGGGCGGGGcc                                            >  2:3683987/1‑101 (MQ=17)
                                                    aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGGA.TGGC..GAA.G.CCAG.AGGGGGGCA.GGGC.CCCCCTGCCCCCGCGGTCAGAGGCGGCGccg                                           >  1:4463056/1‑100 (MQ=12)
                                                     aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                          <  1:4650818/101‑1 (MQ=17)
                                                       ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                        <  1:2623656/101‑1 (MQ=17)
                                                        ccgATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                       <  2:1774723/99‑1 (MQ=17)
                                                         ggATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGTTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      <  1:4552032/101‑1 (MQ=17)
                                                         ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcg                                      >  1:1223598/1‑101 (MQ=17)
                                                         ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                      >  1:3101667/1‑101 (MQ=17)
                                                          gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                     <  1:2311185/101‑1 (MQ=17)
                                                           aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCAGAGC..CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGatcgg                                    >  2:857681/1‑97 (MQ=17)
                                                           aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  2:1430771/101‑1 (MQ=17)
                                                           aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcggg                                    <  1:1172984/101‑1 (MQ=17)
                                                               cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                >  2:84451/1‑101 (MQ=17)
                                                                tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  2:944117/101‑1 (MQ=17)
                                                                tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  1:4605986/101‑1 (MQ=17)
                                                                tttCATCTTCCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                               <  2:4386003/101‑1 (MQ=17)
                                                                 ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                              >  2:1718966/1‑101 (MQ=18)
                                                                  tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                             >  2:3581/1‑100 (MQ=18)
                                                                  tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                             <  2:2564012/101‑1 (MQ=18)
                                                                  tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGAGGCGGCGCCTTGACGGGCGGGGgc                             >  2:2012970/1‑101 (MQ=17)
                                                                   cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                            >  1:3806231/1‑101 (MQ=18)
                                                                     tCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          <  2:2176871/101‑1 (MQ=18)
                                                                     tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                          >  2:4376916/1‑101 (MQ=18)
                                                                        tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCACGAAGGGCCGGGGCGCgggg                       >  1:835590/1‑100 (MQ=9)
                                                                        tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                       <  1:617765/101‑1 (MQ=18)
                                                                        tGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                       <  2:4637959/101‑1 (MQ=18)
                                                                         gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                      >  2:4636090/1‑101 (MQ=18)
                                                                          cccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                     <  2:3538976/101‑1 (MQ=18)
                                                                           ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCAtt                    >  1:2628535/1‑101 (MQ=18)
                                                                           ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  2:2304365/101‑1 (MQ=18)
                                                                           ccGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                    <  1:2122/101‑1 (MQ=18)
                                                                             gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  1:98413/101‑1 (MQ=18)
                                                                             gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                  <  1:56142/101‑1 (MQ=18)
                                                                             gAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGCCCTTCACGGGCGGGGGGGCGGGCATTaa                  >  2:1959993/1‑101 (MQ=12)
                                                                              aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  1:459589/101‑1 (MQ=18)
                                                                              aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                 <  2:4734183/101‑1 (MQ=18)
                                                                               aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  1:1127494/1‑101 (MQ=21)
                                                                               aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                >  2:90304/1‑101 (MQ=21)
                                                                               aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                <  1:2740830/101‑1 (MQ=21)
                                                                                atatCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg               >  1:1296638/1‑101 (MQ=21)
                                                                                  atCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             >  2:1228239/1‑101 (MQ=21)
                                                                                  atCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa             <  1:2278845/101‑1 (MQ=21)
                                                                                  atCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGaa             >  2:2821257/1‑101 (MQ=21)
                                                                                   tCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa            <  1:4150127/101‑1 (MQ=21)
                                                                                       ggggggTGAA.TGGC..GAA.G.CCAG.AGGGCGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc        >  1:4254827/1‑101 (MQ=25)
                                                                                        gggggTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       >  1:316332/1‑101 (MQ=25)
                                                                                        gggggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  1:4292049/101‑1 (MQ=25)
                                                                                        gggggTGAA.TGGC..G.AGG.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa       <  2:417052/101‑1 (MQ=25)
                                                                                          gggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  1:2590566/101‑1 (MQ=25)
                                                                                          gggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  1:749122/101‑1 (MQ=25)
                                                                                          gggTGAA.TGGC..G.A.A.GAGAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc     <  2:71212/101‑1 (MQ=37)
                                                                                           ggTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCaaaa    >  1:16817/1‑99 (MQ=21)
                                                                                           ggTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa    >  2:377256/1‑101 (MQ=25)
                                                                                            gTGAA.TGGC..GAA.GCCAG..AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg   >  1:3642685/1‑101 (MQ=25)
                                                                                                      |                                                                                                   
CGCTGCCCCCGTTCCGCAAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCA.AGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGA  >  NC_014034/2493960‑2494152

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG