Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,076 +C 10.9% intergenic (+124/‑118) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0760.→C10.9% 44.7 ‑0.6 ‑0.6155intergenic (+124/‑118)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (8/5):  ref base (70/68):  total (82/73)
Fisher's exact test strand distribution p-value = 5.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.46e-01

Only 100 of 176 total aligned reads displayed.

AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCAAC  >  NC_014034/2493977‑2494172
                                                                                                                                                                                                            
aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCa                                                                                                   <  1:1431276/101‑1 (MQ=18)
  gCGGACTCCCCCCGGGAAATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCaga                                                                                                 >  1:4734439/1‑99 (MQ=18)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAC.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                                               >  1:4638215/1‑97 (MQ=17)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                                               >  2:172563/1‑97 (MQ=18)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGACCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagcgc                                                                                               >  1:2769520/1‑97 (MQ=17)
     gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cc                                                                                            >  1:2725543/1‑101 (MQ=17)
      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGCagcggcc                                                                                             >  2:2348947/1‑95 (MQ=17)
      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.ccc                                                                                           >  1:283443/1‑101 (MQ=18)
      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.ccc                                                                                           >  2:3025943/1‑101 (MQ=18)
       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCG.cccc                                                                                          <  1:402027/101‑1 (MQ=17)
       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cccc                                                                                          <  2:196547/101‑1 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.ccccc                                                                                         >  1:806307/1‑101 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.ccccc                                                                                         >  2:128706/1‑101 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.ccccc                                                                                         >  1:1565968/1‑101 (MQ=18)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cccccct                                                                                       <  2:848858/101‑3 (MQ=17)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCG.cccccct                                                                                       <  1:4471390/101‑3 (MQ=17)
              cGGAATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGccc                                                                                    <  1:2203745/101‑1 (MQ=17)
               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGcccc                                                                                   >  1:3846964/1‑101 (MQ=17)
               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGcccc                                                                                   >  1:3588006/1‑101 (MQ=17)
               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGcccc                                                                                   <  2:191057/101‑1 (MQ=17)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcg                                                                                  >  1:659612/1‑101 (MQ=2)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgc                                                                                 >  1:4404457/1‑100 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                                >  1:2418385/1‑99 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgcc                                                                                <  2:3882519/101‑3 (MQ=2)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcggg                                                                                >  1:3337824/1‑100 (MQ=17)
                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCA.AGAGGGGGCAGCG.CCCCCTGCCCcgccg                                                                               >  2:1139753/1‑101 (MQ=2)
                    atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcggcgg                                                                              >  2:1445592/1‑101 (MQ=18)
                     tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCcggcggc                                                                             >  2:977702/1‑100 (MQ=17)
                       tGCAGCAATATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                                           >  1:3303403/1‑101 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                                           >  2:263673/1‑101 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTcc                                                                           <  1:3190927/101‑1 (MQ=17)
                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                                        <  1:2414691/101‑1 (MQ=17)
                          agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                                        <  1:998655/101‑1 (MQ=17)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGG.AAGCGCCCCCCTGCCCCGGCGGTCCGTggc                                                                      >  1:345412/1‑101 (MQ=18)
                             aaGATCAAACCGGATGCCTTTCATCTTGCCAGAAATCGCCAGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                     <  1:836859/101‑1 (MQ=9)
                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                                <  2:4421536/101‑1 (MQ=17)
                                  gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGG.AAGCGCCCCCCTGCCCCGGCGGTCCGGGGCGGGGcc                                                                >  2:3683987/1‑101 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGGA.TGGC..GAA.G.CCAG.AGGGGGGCAGGGC.CCCCCTGCCCCCGCGGTCAGAGGCGGCGccg                                                               >  1:4463056/1‑100 (MQ=12)
                                    aaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCtt                                                              <  1:4650818/101‑1 (MQ=17)
                                     aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                             >  1:2685816/1‑101 (MQ=18)
                                      ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                            <  1:2623656/101‑1 (MQ=17)
                                       ccgATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                           <  2:1774723/99‑1 (MQ=17)
                                       cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                           <  2:4640867/101‑1 (MQ=17)
                                        ggATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGTTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                                          <  1:4552032/101‑1 (MQ=17)
                                           tgctttacgtCTTGCCCGACATATTCCGGGGGGCAAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCCTGAcgggg                                                       >  2:142639/10‑100 (MQ=9)
                                             ccTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcgg                                                     <  1:1254947/101‑1 (MQ=17)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTTCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                   >  1:2587620/1‑101 (MQ=17)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                   <  1:4605986/101‑1 (MQ=17)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGCG..A.A.A.CCAA.AGGGGGGCCAGGGCCCCCCGGCCCCGGCGG.CCGGGGCCGGGCCCTTGCCGGCggggg                                                  >  1:1221501/1‑101 (MQ=9)
                                               tttCATCTTCCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                   <  2:4386003/101‑1 (MQ=17)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggg                                                 >  2:3581/1‑100 (MQ=18)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                                 <  2:4272800/101‑1 (MQ=18)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                                 <  2:2564012/101‑1 (MQ=18)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGAGGCGGCGCCTTGACGGGCGGGGgc                                                 >  2:2012970/1‑101 (MQ=17)
                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcg                                                >  1:3806231/1‑101 (MQ=18)
                                                   aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCGCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggggggg                                               >  1:4009401/1‑98 (MQ=17)
                                                    tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                                              >  2:4376916/1‑101 (MQ=18)
                                                     cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                                             >  2:1633663/1‑101 (MQ=18)
                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                                           <  1:617765/101‑1 (MQ=18)
                                                        gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                          >  2:4636090/1‑101 (MQ=18)
                                                        gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                          <  2:2094526/101‑1 (MQ=18)
                                                         cccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                                         <  2:3538976/101‑1 (MQ=18)
                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCTTGACGGGCGGGGGCGCGGGCAtt                                        >  1:2628535/1‑101 (MQ=18)
                                                          ccGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                        <  1:2122/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGCGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGCCCTTCACGGGCGGGGGGGCGGGCATTaa                                      >  2:1959993/1‑101 (MQ=12)
                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                    >  1:1127494/1‑101 (MQ=21)
                                                              aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                    >  2:90304/1‑101 (MQ=21)
                                                                taccccGGGGGGTGAA.TGGC..G.A.A.GCCA.GAGGGGGCCAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                  <  2:1329142/98‑1 (MQ=21)
                                                                 atCCGGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                                 <  2:81842/101‑1 (MQ=21)
                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                                 >  2:1228239/1‑101 (MQ=21)
                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                                 <  1:2278845/101‑1 (MQ=21)
                                                                 atCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGG.GAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGCGCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                                 >  1:538066/1‑101 (MQ=16)
                                                                 atCCCGGGGGGGGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGaa                                 >  2:2821257/1‑101 (MQ=21)
                                                                  tCCCGGGGGGTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                                <  1:4150127/101‑1 (MQ=21)
                                                                      ggggggTGAA.TGGC..GAA.G.CCAG.AGGGCGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                            >  1:4254827/1‑101 (MQ=25)
                                                                       gggggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                           <  1:4292049/101‑1 (MQ=25)
                                                                       gggggTGAA.TGGC..G.AGG.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                           <  2:417052/101‑1 (MQ=25)
                                                                         gggTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                         >  1:3130257/1‑101 (MQ=25)
                                                                         gggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                         <  1:749122/101‑1 (MQ=25)
                                                                         gggTGAA.TGGC..G.A.A.GAGAGAGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                         <  2:71212/101‑1 (MQ=37)
                                                                          ggTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCaaaa                        >  1:16817/1‑99 (MQ=21)
                                                                          ggTGAA.TGGC..GAA.GCCAG..AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                        >  2:377256/1‑101 (MQ=25)
                                                                          ggTGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                        <  1:2920432/101‑1 (MQ=25)
                                                                            tGAA.TGGC..GAA.G.CCAG.AGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa                      <  1:2132218/101‑1 (MQ=25)
                                                                                        gcgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc                <  1:3228501/91‑1 (MQ=38)
                                                                                        gcgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAACGAAATTGCAACAGAtgctgc                >  2:547187/11‑101 (MQ=37)
                                                                                         cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg               >  2:3657279/10‑101 (MQ=38)
                                                                                         cgaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg               <  2:212077/92‑1 (MQ=38)
                                                                                          gaagccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa              >  1:1753739/9‑101 (MQ=38)
                                                                                           gaagccagggggggGCAGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa              <  1:4583447/93‑1 (MQ=255)
                                                                                            agccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTTCAACAGATGCTGCGATc            >  1:94676/7‑101 (MQ=38)
                                                                                              ccagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGGATTAACCGGAATTGCAACAGCTGCTGCGATCCt          >  2:2927109/5‑101 (MQ=37)
                                                                                               cagaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc         >  1:81612/4‑101 (MQ=38)
                                                                                                agaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg        >  1:2957621/3‑101 (MQ=39)
                                                                                                 gaGGGGGGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc       >  2:2223775/2‑101 (MQ=39)
                                                                                                   ggggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc     >  2:4690776/1‑101 (MQ=255)
                                                                                                   ggggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc     <  2:806234/101‑1 (MQ=255)
                                                                                                   ggggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc     <  1:3097638/101‑1 (MQ=255)
                                                                                                   ggggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc     <  1:3169857/101‑1 (MQ=255)
                                                                                                    gggggC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAACCTCGCCCa    <  1:1079081/101‑1 (MQ=255)
                                                                                                                                                                                                            
AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGA.AGGGGGGCAAGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCAAC  >  NC_014034/2493977‑2494172

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG