BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,494,080	+C	91.0%	intergenic (+128/‑114)	RCAP_rcc02319 → / → RCAP_rcc02320	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,494,080	1	.→C	91.0%	34.8 ‑0.6 ‑0.1	145	intergenic (+128/‑114)	RCAP_rcc02319/RCAP_rcc02320	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (67/64): ref base (8/5): total (76/69)
Fisher's exact test strand distribution p-value = 5.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.62e-01

Only 100 of 166 total aligned reads displayed.


GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCA.AGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCAAC  >  NC_014034/2493982‑2494172


gACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCG.cc                                                                                            >  1:2725543/1‑101 (MQ=17)


 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCG.ccc                                                                                           >  2:3025943/1‑101 (MQ=18)


 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCG.ccc                                                                                           >  1:283443/1‑101 (MQ=18)


 aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCG.ccc                                                                                           >  2:2414556/1‑101 (MQ=18)


  cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.GCCAG.AGGGGGGC..AGCG.cccc                                                                                          <  2:2954777/101‑1 (MQ=17)


  cTCCCCCCGGGATAGTTGCAGCAAGATGAATCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCG.cccc                                                                                          <  2:43056/101‑1 (MQ=17)


   tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCG.ccccc                                                                                         >  1:806307/1‑101 (MQ=17)


     cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCG.cccccct                                                                                       <  1:4471390/101‑3 (MQ=17)


       cccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGACCCCCTGc                                                                                      >  2:1978451/1‑101 (MQ=17)


         cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCATAGGGGGGC..AGCGCCCCCCTGccc                                                                                    >  1:2925682/1‑101 (MQ=18)


          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGcccc                                                                                   <  2:191057/101‑1 (MQ=17)


          gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGcccc                                                                                   >  1:3846964/1‑101 (MQ=17)


           ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGC.AGCG.CCCCCTGCCCcg                                                                                  <  1:4015443/101‑1 (MQ=0)


            gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGC.AGCG.CCCCCTGCCCcgc                                                                                 >  1:4404457/1‑100 (MQ=2)


             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGC.AGCG.CCCCCTGCCCcgcc                                                                                >  1:2418385/1‑99 (MQ=2)


             atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGC.AGCG.CCCCCTGCCCcgcc                                                                                <  2:3882519/101‑3 (MQ=2)


              tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGC.AGCG.CCCCCTGCCCcgccg                                                                               >  2:1139753/1‑101 (MQ=2)


              tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCcggcg                                                                               >  1:1636751/1‑101 (MQ=18)


               atTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCcggcgg                                                                              >  2:1445592/1‑101 (MQ=18)


                tttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGC.AGCG.CCCCCTGCCCcgccgga                                                                             >  2:2605858/1‑100 (MQ=2)


                tttGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.A.G.GCCAAGAGGGGGC.AGCG.CCCCCTGCCCcgccgga                                                                             >  2:2878051/1‑100 (MQ=2)


                tttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCcggcggc                                                                             >  2:977702/1‑100 (MQ=17)


                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTcc                                                                           >  2:263673/1‑101 (MQ=17)


                  tGCAGCAAGATGAAACCGGATGCCTTTCATCTGGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTcc                                                                           <  1:3190927/101‑1 (MQ=17)


                    cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGt                                                                         <  1:4346247/101‑1 (MQ=17)


                     agcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTg                                                                        <  1:2414691/101‑1 (MQ=17)


                       caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGG.A.AGCGCCCCCCTGCCCCGGCGGTCCGTggc                                                                      >  1:345412/1‑101 (MQ=18)


                        aaGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGCCCGTggcg                                                                     >  1:34950/1‑101 (MQ=17)


                        aaGATCAAACCGGATGCCTTTCATCTTGCCAGAAATCGCCAGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTggcg                                                                     <  1:836859/101‑1 (MQ=9)


                           aTGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGgcggcg                                                                  >  2:1882578/1‑101 (MQ=18)


                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                                <  2:4421536/101‑1 (MQ=17)


                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGcc                                                                >  1:2626861/1‑101 (MQ=17)


                             gAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGG.A.AGCGCCCCCCTGCCCCGGCGGTCCGGGGCGGGGcc                                                                >  2:3683987/1‑101 (MQ=17)


                              aaaCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGGGGA.TGGC..GAA.G.CCAGAGGGGGGCA.GGGC.CCCCCTGCCCCCGCGGTCAGAGGCGGCGccg                                                               >  1:4463056/1‑100 (MQ=12)


                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                             <  1:3335693/101‑1 (MQ=17)


                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                             >  1:812992/1‑101 (MQ=17)


                                aCCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                             >  1:2685816/1‑101 (MQ=18)


                                 ccGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGa                                                            <  1:2623656/101‑1 (MQ=17)


                                  cGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAc                                                           <  2:1041092/101‑1 (MQ=17)


                                   ggATGCCTTTCATCTTGCCCGAAATGTCCCGGGGGTTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                                          <  1:4552032/101‑1 (MQ=17)


                                   ggATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCcg                                                          >  1:1223598/1‑101 (MQ=17)


                                     aTGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGCAGAGC..CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGatcgg                                                        >  2:857681/1‑97 (MQ=17)


                                      tgctttacgtCTTGCCCGACATATTCCGGGGGGCAAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCCTGAcgggg                                                       >  2:142639/10‑100 (MQ=9)


                                      tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                       <  1:4037518/101‑1 (MQ=17)


                                         cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                    >  2:84451/1‑101 (MQ=17)


                                         cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAA.G.CCAGAGGGGGGCA.GCAC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGGCGTGAcgggcggg                                                    >  2:4502618/1‑101 (MQ=17)


                                          tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTTCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                   >  1:2587620/1‑101 (MQ=17)


                                          tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                   <  1:4605986/101‑1 (MQ=17)


                                          tttCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGCG..A.A.A.CCAAAGGGGGGCC.AGGGCCCCCCGGCCCCGGCGG.CCGGGGCCGGGCCCTTGCCGGCggggg                                                  >  1:1221501/1‑101 (MQ=9)


                                          tttCATCTTCCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                   <  2:4386003/101‑1 (MQ=17)


                                            tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                                 <  2:4272800/101‑1 (MQ=18)


                                            tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGAGGCGGCGCCTTGACGGGCGGGGgc                                                 >  2:2012970/1‑101 (MQ=17)


                                               tCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                                              <  2:2176871/101‑1 (MQ=18)


                                               tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                                              >  1:46093/1‑101 (MQ=18)


                                                cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCgg                                             >  2:1633663/1‑101 (MQ=18)


                                                  tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGGCCACGAAGGGCCGGGGCGCgggg                                           >  1:835590/1‑100 (MQ=9)


                                                  tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                                           <  1:617765/101‑1 (MQ=18)


                                                  tGCCCGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                                           <  2:4637959/101‑1 (MQ=18)


                                                   gCCCGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                          <  2:2094526/101‑1 (MQ=18)


                                                    cccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAt                                         <  2:3538976/101‑1 (MQ=18)


                                                     ccGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                        <  2:3593736/101‑1 (MQ=18)


                                                     ccGAAATACCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                        <  1:2122/101‑1 (MQ=18)


                                                      cGAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTa                                       <  2:577359/101‑1 (MQ=18)


                                                       gAAATATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                      <  1:98413/101‑1 (MQ=18)


                                                        aaaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAc                                     <  2:4734183/101‑1 (MQ=18)


                                                         aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                    >  2:90304/1‑101 (MQ=21)


                                                         aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                    >  1:1127494/1‑101 (MQ=21)


                                                         aaTATCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAAcc                                    <  1:2740830/101‑1 (MQ=21)


                                                          atatCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCg                                   >  1:1296638/1‑101 (MQ=21)


                                                           taccccGGGGGGTGAA.TGGC..G.A.A.GCCAGAGGGGGCC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                  <  2:1329142/98‑1 (MQ=21)


                                                            atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                                 <  1:2278845/101‑1 (MQ=21)


                                                            atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                                 >  2:1228239/1‑101 (MQ=21)


                                                            atCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGG.G.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGGCGCTTGACGGGCGGGGGCGCGGGCATTAACCGaa                                 >  1:538066/1‑101 (MQ=16)


                                                             tCCCGGGGGGTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                                <  1:4150127/101‑1 (MQ=21)


                                                                 ggggggTGAA.TGGC..GAA.G.CCAGAGGGCGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                            >  1:4254827/1‑101 (MQ=25)


                                                                  gggggTGAA.TGGC..GAA.GCCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                           >  1:316332/1‑101 (MQ=25)


                                                                  gggggTGAA.TGGC..G.AGG.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                           <  2:417052/101‑1 (MQ=25)


                                                                    gggTGAA.TGGC..GAA.GCCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                         >  1:3130257/1‑101 (MQ=25)


                                                                    gggTGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAAc                         <  1:749122/101‑1 (MQ=25)


                                                                     ggTGAA.TGGC..GAA.GCCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCGGGCATTAACCGAAATTGCaaaa                        >  1:16817/1‑99 (MQ=21)


                                                                     ggTGAA.TGGC..GAA.GCCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACa                        >  2:377256/1‑101 (MQ=25)


                                                                      gTGAA.TGGC..GAA.GCCAG.AGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                       >  1:3642685/1‑101 (MQ=25)


                                                                       tGAA.TGGC..GAA.G.CCAGAGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGa                      <  1:2132218/101‑1 (MQ=25)


                                                                                  gcgaagccagaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc                <  1:3228501/91‑1 (MQ=38)


                                                                                   cgaagccagaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg               >  2:3657279/10‑101 (MQ=38)


                                                                                    gaagccagaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa              >  1:1753739/9‑101 (MQ=38)


                                                                                     gaagccagggggggGC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa              <  1:4583447/93‑1 (MQ=255)


                                                                                      agccagaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc            >  1:3827434/7‑101 (MQ=38)


                                                                                         cagaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc         <  2:782248/98‑1 (MQ=38)


                                                                                         cagaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc         >  2:1961035/4‑101 (MQ=38)


                                                                                          agaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCg        >  1:2957621/3‑101 (MQ=39)


                                                                                           gaGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGc       >  2:2223775/2‑101 (MQ=39)


                                                                                            aGGGGGGC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGcc      >  1:624446/1‑101 (MQ=255)


                                                                                             ggggggC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc     <  2:806234/101‑1 (MQ=255)


                                                                                             ggggggC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc     >  2:4690776/1‑101 (MQ=255)


                                                                                             ggggggC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGccc     <  1:3097638/101‑1 (MQ=255)


                                                                                              gggggC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa    >  1:3266503/1‑101 (MQ=255)


                                                                                              gggggC..AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGAACCTCGCCCa    <  1:1079081/101‑1 (MQ=255)


                                                                                               ggggCA.GCCC.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCaa   <  2:1753634/101‑1 (MQ=255)


                                                                                                 ggCC.AGCGCCCCCCTGCCCCGGCGGTCCGTGGCGGTGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCAAc  <  2:1294270/101‑1 (MQ=255)


GACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.A.G.CAGAAGGGGGGCA.AGCG.CCCCCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCAAC  >  NC_014034/2493982‑2494172

Base quality scores:


ATCG

< 3 ≤


ATCG

< 15 ≤


ATCG

< 24 ≤


ATCG

< 32 ≤


ATCG

< 40 ≤


ATCG