Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,536 +G 100% intergenic (+8/+186) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,5361.→G100.0% 468.3 161intergenic (+8/+186)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (38/121):  ref base (1/1):  total (39/122)

Only 100 of 201 total aligned reads displayed.

GCCGAGGCGCAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGGGGCGCTGCCCCCCTC.C.T..G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAA  >  NC_014034/2566436‑2566629
                                                                                                      |                                                                                                   
gCCGAGGCGCAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAc                                                                                                      >  2:2503885/1‑101 (MQ=255)
gCCGAGGCGCAACAGCGCGCCTATGCAATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAc                                                                                                      <  1:2854668/101‑1 (MQ=255)
  cGAGGCGCAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCCCTGGGACG.AAAA.gg                                                                                                   >  1:1666502/1‑101 (MQ=255)
  cGAGGCGCAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.gg                                                                                                   >  2:400269/1‑101 (MQ=255)
   gAGGCGCAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAccag                                                                                                   >  2:4697466/1‑98 (MQ=255)
   gAGGCGCAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCCCTGAGACG.AAAC.ggg                                                                                                  >  2:2350057/1‑101 (MQ=255)
      gcgcAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCTGCTGGCGCGCGCTCTGAGACG.AAAC.gggggg                                                                                               <  1:4088477/101‑2 (MQ=255)
      gcgcAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGTCG.AAAC.gggggg                                                                                               <  1:2567407/101‑2 (MQ=255)
      gcgcAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.gggggg                                                                                               <  2:2864707/101‑2 (MQ=255)
      gcgcAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.gggggg                                                                                               <  2:4317275/101‑2 (MQ=255)
       cgcAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.ggggggc                                                                                              <  2:1958653/101‑3 (MQ=255)
        gcAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.gggggggg                                                                                             >  2:1536562/1‑98 (MQ=255)
         cAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGGGCGCTCTGGGACG.AAAC.ggggggggg                                                                                            >  1:2087784/1‑97 (MQ=255)
          aaCAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCTCTGAGACG.AAAC.ggggggggcg                                                                                           >  1:1898895/1‑96 (MQ=255)
          aaCAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.ggggggcgct                                                                                           >  2:3105090/1‑96 (MQ=255)
               cgcgcCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGcccc                                                                                       <  1:544094/101‑1 (MQ=255)
                gcgcCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGgcccc                                                                                      >  1:2925980/1‑101 (MQ=255)
                gcgcCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGccccc                                                                                      <  2:1444278/101‑1 (MQ=255)
                 cgcCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGcccccc                                                                                     <  2:4431794/101‑1 (MQ=255)
                    cTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCcctct                                                                                  <  2:2189065/101‑2 (MQ=255)
                     tATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGcccccccctt                                                                                 >  2:3710117/1‑97 (MQ=255)
                     tATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCcctctt                                                                                 <  2:2724138/101‑3 (MQ=255)
                     tATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCcctctt                                                                                 <  2:1485110/101‑3 (MQ=255)
                     tATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCcctctt                                                                                 >  2:3157141/1‑99 (MQ=255)
                     tATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCcctctt                                                                                 <  1:303991/101‑3 (MQ=255)
                         cGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGccccccccttgggc                                                                             >  2:2835397/1‑93 (MQ=255)
                          gATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCCCCGTGACGCAAAG.GGGGGGGCAGCCCCcccc.cctggcc                                                                           >  1:240302/1‑95 (MQ=255)
                              gTCGACGCGATCGTCTGGCACGAGGGGTTCTGCCGCCGCGACAGCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..ggccttcgg                                                                    <  2:724236/101‑9 (MQ=25)
                                cGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGcc                                                                  <  1:3105290/101‑1 (MQ=37)
                                   tGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAAt                                                               <  1:621101/101‑1 (MQ=37)
                                    gCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCGGGCGCGCGCTCTGAGACG.AAACGGGGGGGGCTGCCCCCCTC.T.T..GGCCTTCGGCCaaat                                                              >  2:2427360/1‑99 (MQ=25)
                                         cGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCAccc                                                         >  2:2132920/1‑101 (MQ=37)
                                           tcTGGCCCGAGGGGTTCTGCCGCCGCGACGTCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCAccccc                                                       <  2:2697626/100‑1 (MQ=25)
                                            cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGGGCGCGCCCTGAGACGAAAAG.GGGGGGGCCGCCCCCCCC.CTT..GC.CCTCGGCCCATTTAcccccc                                                      >  1:1782594/1‑101 (MQ=255)
                                            cTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCAcccccc                                                      <  1:341792/101‑1 (MQ=25)
                                             tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCCGCCCCCCTC.T.TGGG..CCTCGGCCCATTCACCCCCCg                                                     >  2:4151195/1‑101 (MQ=255)
                                             tGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCACTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCg                                                     <  1:3484577/101‑1 (MQ=25)
                                              ggCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCgg                                                    <  1:1271329/101‑1 (MQ=25)
                                              ggCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCgg                                                    <  2:2107644/101‑1 (MQ=25)
                                               gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCggg                                                   <  1:4648669/101‑1 (MQ=25)
                                               gCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCggg                                                   <  2:4590998/101‑1 (MQ=25)
                                                cccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGa                                                  <  2:3361022/101‑1 (MQ=25)
                                                 ccGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCCCTT.T..GC.CTTCGGCCAATTCACCCCCCCGGaa                                                 >  1:4178037/1‑100 (MQ=25)
                                                   gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGatat                                               <  1:3598415/101‑1 (MQ=25)
                                                   gAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGatat                                               <  1:2293697/101‑1 (MQ=25)
                                                     ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCCATTCACCCCCCCGGGTAttt                                             >  2:4042853/1‑101 (MQ=21)
                                                     ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                             <  2:137329/101‑1 (MQ=21)
                                                     ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATAttt                                             <  2:1119688/101‑1 (MQ=21)
                                                     ggggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCCCTGAGACG.AAACGGGGGGGGCTGCCCCCCTC.T.T..GGCCCTCCGCCACTTCAcccccccgcagaatt                                             >  2:1761936/1‑92 (MQ=19)
                                                      gggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTc                                            <  1:4680640/101‑1 (MQ=21)
                                                       ggTTCTTCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCg                                           <  1:777211/101‑1 (MQ=21)
                                                       ggTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCg                                           <  1:2504029/101‑1 (MQ=21)
                                                        gTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCgg                                          >  2:4461070/1‑101 (MQ=21)
                                                        gTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCgg                                          <  2:3424315/101‑1 (MQ=21)
                                                         ttCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCCGCCCATTCACCCCCCcgggcaattttgg                                         >  1:863966/1‑91 (MQ=19)
                                                             gccgccGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGCCaga                                     >  2:3688659/1‑99 (MQ=21)
                                                               cgccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAt                                   <  2:302121/101‑1 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTCTT.T..GC.CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  >  2:3639782/1‑101 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  <  2:3280760/101‑1 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  <  2:500633/101‑1 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  <  2:852108/101‑1 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  <  1:2697250/101‑1 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  <  1:2800218/101‑1 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  <  1:3854907/101‑1 (MQ=21)
                                                                gccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                  <  1:734598/101‑1 (MQ=21)
                                                                 ccgcGACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                 <  2:3892920/101‑1 (MQ=21)
                                                                    cgACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                              <  2:1051078/101‑1 (MQ=21)
                                                                     gACATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCCGGGAATTTCGGGCCAGATAaaaaa                             >  1:3759032/1‑101 (MQ=18)
                                                                       cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                           <  1:1439699/101‑1 (MQ=21)
                                                                       cATCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                           >  2:3489264/1‑101 (MQ=21)
                                                                        aTCGGCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGt                          <  1:1860053/101‑1 (MQ=21)
                                                                           ggCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtt                       <  1:1065005/101‑1 (MQ=21)
                                                                           ggCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCtt                       >  1:631974/1‑101 (MQ=21)
                                                                            gCTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCttt                      <  1:1973337/101‑1 (MQ=21)
                                                                             cTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                     <  1:4716385/101‑1 (MQ=21)
                                                                             cTGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCtttt                     <  2:3399392/101‑1 (MQ=21)
                                                                              tGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                    <  1:2252535/101‑1 (MQ=21)
                                                                              tGGCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                    <  2:2288983/101‑1 (MQ=21)
                                                                               ggCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                   <  2:588115/101‑1 (MQ=21)
                                                                               ggCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                   >  1:4448274/1‑101 (MQ=21)
                                                                               ggCGCGCGCTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                   <  2:3467003/101‑1 (MQ=21)
                                                                                 cgcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                 <  2:2227890/101‑1 (MQ=21)
                                                                                 cgcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                 <  2:487191/101‑1 (MQ=21)
                                                                                 cgcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                 <  1:2001870/101‑1 (MQ=21)
                                                                                 cgcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                 <  2:1685975/101‑1 (MQ=21)
                                                                                  gcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                <  2:3006693/101‑1 (MQ=21)
                                                                                  gcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                <  1:3978199/101‑1 (MQ=21)
                                                                                  gcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                >  1:4676021/1‑101 (MQ=21)
                                                                                  gcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                <  2:1839728/101‑1 (MQ=21)
                                                                                  gcgcgcTCTGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                <  2:1689403/101‑1 (MQ=21)
                                                                                        tctGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCtt          <  2:2700968/101‑1 (MQ=21)
                                                                                          tGAGACG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAAATCACCCCCCGGGACACTTCGTGCAACACGAAAAAGGGATTTTTCTTTTATCttcc        >  1:4520337/1‑99 (MQ=2)
                                                                                           gagaCG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGcc       <  2:3940742/101‑1 (MQ=21)
                                                                                            agaCG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccc      <  2:1243486/101‑1 (MQ=21)
                                                                                             gaCG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGAGATTTTGGGCAAGATAAAGAAGGGGTTTTCCTTTCCTCTTGCCCg     >  2:3585813/1‑101 (MQ=18)
                                                                                             gaCG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGAAATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGccca     >  2:1883798/1‑100 (MQ=21)
                                                                                               cG.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaa   <  1:4319021/101‑1 (MQ=21)
                                                                                                g.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa  >  2:4211402/1‑101 (MQ=21)
                                                                                                g.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa  >  1:2914501/1‑101 (MQ=21)
                                                                                                g.AAACGGGGGGCGCTGCCCCCCTC.T.T..GGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGaaa  >  1:4378135/1‑101 (MQ=21)
                                                                                                      |                                                                                                   
GCCGAGGCGCAACAGCGCGCCTATGCGATGGTCGATGCGATCGACTGGCCCGAGGGGTTCTGCCGCCGCGACATCGGCTGGCGCGCGCTCTGAGACG.AAAC.GGGGGCGCTGCCCCCCTC.C.T..G..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATCTTGCCCGAAA  >  NC_014034/2566436‑2566629

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG