Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,577,283 +G 100% intergenic (+205/‑224) fdx → / → purU ferredoxin VI/formyltetrahydrofolate deformylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,577,2831.→G100.0% 11.9 94intergenic (+205/‑224)fdx/purUferredoxin VI/formyltetrahydrofolate deformylase
Reads supporting (aligned to +/- strand):  new base (69/19):  ref base (1/5):  total (70/24)
Fisher's exact test strand distribution p-value = 3.82e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.82e-02

Only 100 of 122 total aligned reads displayed.

CGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGCAA  >  NC_014034/2577183‑2577378
                                                                                                        |                                                                                                 
cGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTc.c                                                                                                    <  1:4294438/101‑1 (MQ=255)
cGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GAGGGTc.c                                                                                                    <  1:419884/101‑1 (MQ=255)
 ggCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..g                                                                                                 <  1:1263682/101‑1 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gg                                                                                                <  2:3808479/101‑1 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gg                                                                                                <  1:3444063/101‑1 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gg                                                                                                >  1:3421550/1‑101 (MQ=255)
  gCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gg                                                                                                >  1:2095599/1‑101 (MQ=255)
   cGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..ggg                                                                                               <  1:1052184/101‑1 (MQ=255)
    gTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gggg                                                                                              >  2:2344577/1‑101 (MQ=255)
     tATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..ggggg                                                                                             >  1:652759/1‑100 (MQ=255)
     tATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..ggggg                                                                                             >  1:4515406/1‑100 (MQ=255)
      aTTTGCACCAGGAACAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gggggc                                                                                            <  1:4096182/101‑3 (MQ=255)
      aTTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gggggc                                                                                            <  2:595776/101‑3 (MQ=255)
      aTTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gggggc                                                                                            >  1:636432/1‑99 (MQ=255)
       tttGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gggggcg                                                                                           >  2:800/1‑98 (MQ=255)
        ttGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gggggcgg                                                                                          <  1:1137736/101‑5 (MQ=255)
         tGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..gggggcgga                                                                                         <  2:3860717/101‑6 (MQ=255)
          gCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAg                                                                                         <  1:4485444/101‑1 (MQ=255)
          gCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAg                                                                                         <  2:215157/101‑1 (MQ=255)
               aGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.ccccc                                                                                   <  1:929108/101‑1 (MQ=255)
               aGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.ccccc                                                                                   <  2:2813127/101‑1 (MQ=255)
                ggAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGCCCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCcg                                                                                  <  1:699074/101‑1 (MQ=255)
                 gAAAAGCCAAAGCCGTCCCCGGCAGGACCTAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCcgg                                                                                 >  1:1573741/1‑101 (MQ=255)
                   aaagccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCcggcg                                                                               <  2:581089/101‑1 (MQ=255)
                   aaagccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCcggcg                                                                               <  1:3700132/101‑1 (MQ=255)
                    aagccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCcggcgg                                                                              >  1:3863971/1‑101 (MQ=255)
                       ccaaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTc                                                                           <  1:3942079/101‑1 (MQ=255)
                         aaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCggggtgc                                                                         >  2:3529969/1‑97 (MQ=255)
                         aaagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGc                                                                         >  1:1743070/1‑101 (MQ=255)
                         aaagccGTCCCCGGCAGGACCGAAGGCCTCAGGA.CCCGGGCCCC.TTTTATCTTGCCCCAAATATCCCGGGGGGCC.C..GGGGCGGAG.CCCCCCGCGGGGCggc                                                                        >  2:3420314/1‑99 (MQ=21)
                          aagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCggggggcc                                                                        >  1:1820594/1‑96 (MQ=255)
                          aagccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGcc                                                                        <  1:3054691/101‑1 (MQ=255)
                           agccGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGGCg                                                                       >  2:635937/1‑101 (MQ=25)
                              cGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCgggg                                                                    >  1:203018/1‑101 (MQ=255)
                               gTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGc                                                                   >  1:684558/1‑101 (MQ=255)
                                gccccGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCg                                                                  <  2:2934604/100‑1 (MQ=255)
                                 cccgGGCGGCACCCAAGGCCTGGGGACCCAGGGCCCC.TTTTCTCTTCCCAAAAATATCCC.GGGGGTCGG..GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGa                                                                 <  2:3474092/101‑1 (MQ=12)
                                  cccGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGaa                                                                <  2:3858220/101‑1 (MQ=255)
                                  cccGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGaa                                                                >  2:1905487/1‑101 (MQ=255)
                                  cccGGCAGGACCGAAGCCCTCAGGCCCCCGGCCCCC.TTTTATCTTGCCCAAAATACCCG.GGGGGTC.CG.GGGGGGGAG.CCCCCGGCGGGTCGCCGGGGCGaa                                                                <  1:3826897/101‑1 (MQ=21)
                                    cGGCAGGACC.AAAGCCCCAAGGCCCCGGGCCCCTTTTTTTCTTGCCAAAAAT.TCCC.GGGGGTC.CGGGGGGCGGCC.CCCCCGGCGGGTCGCCGGGGCGAAGc                                                              <  2:3472970/101‑1 (MQ=12)
                                      gCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCCGGGCGAAGCcg                                                            >  2:1685886/1‑101 (MQ=255)
                                        aGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCcgcg                                                          >  1:4263776/1‑101 (MQ=255)
                                        aGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCcgcg                                                          >  1:1918008/1‑101 (MQ=255)
                                         ggACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGGCgcgg                                                         >  1:3279423/1‑101 (MQ=25)
                                         ggACCGAAGCCCTCAGGCCCCCGGGCCCT.TTTTATCTTGCCCAAAATATCCC.GGGGGTCGG..GGGGGGGAC.CCCCCGGCGGGTCGCCGGGGCGAAGCCgcgg                                                         <  1:4240080/101‑1 (MQ=21)
                                          gACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGGGGAGACgcggc                                                        >  1:2778507/1‑101 (MQ=21)
                                             cGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGCGaa                                                     >  1:373936/1‑101 (MQ=255)
                                              gAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGGGCCGGGGGGGGGGCGCGGCGcaa                                                    >  2:1265281/1‑101 (MQ=18)
                                              gAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGGGCCGGGGGGGAGGCGCGGCGcaa                                                    >  1:1994482/1‑101 (MQ=21)
                                               aaGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAAt                                                   >  1:2186027/1‑101 (MQ=255)
                                               aaGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAAt                                                   >  1:2703919/1‑101 (MQ=255)
                                                     tCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGGAATcccggc                                             >  2:159581/1‑101 (MQ=255)
                                                     tCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggc                                             >  2:3731615/1‑101 (MQ=255)
                                                      cAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATcccggcc                                            >  1:535245/1‑101 (MQ=255)
                                                       aGGACCCCGGGCCCC.TTTTATCTTGCCTAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggccc                                           >  2:2759275/1‑101 (MQ=255)
                                                       aGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGCCGCCGGGGCGAACCCCCGGCGAAATcccggccc                                           >  2:2833540/1‑101 (MQ=21)
                                                        ggACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggcccc                                          >  1:1647232/1‑101 (MQ=255)
                                                         gACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATcccggccccg                                         >  1:4565062/1‑101 (MQ=255)
                                                          aCCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGGGAAACGGCGGTGAAAAtccggccccgg                                        >  1:2561616/1‑101 (MQ=18)
                                                            cccGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCa                                      >  1:1797455/1‑101 (MQ=255)
                                                              cGGGCCCT.TTTTATCTTGCCCAAAATACCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAg                                    <  1:1284176/101‑1 (MQ=21)
                                                                ggCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGACGCCGCGGCGGAATCCCGGCCCCGGGAAccg                                  >  1:973464/1‑101 (MQ=21)
                                                                   ccc.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGccg                               >  1:2162120/1‑100 (MQ=255)
                                                                    cctttttATCTTGCCAAAAATTCCCC.GGGGGTCGG..GGGGGGGAC.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCt                               <  2:4580088/98‑1 (MQ=21)
                                                                     ctttttATCTTGCCAAAAATACCCG.GGGGGCC.G..GGGGGGGGGCCCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTg                              <  1:4150978/99‑1 (MQ=21)
                                                                       ttttATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGACGCCGGGGCGAAACCGCGGCGAAATACCGGCCCCGGCACGCGCCGggg                            >  1:3470718/1‑101 (MQ=18)
                                                                        tttATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCCGGAAGCGCCTGGGc                           >  2:1796422/1‑101 (MQ=21)
                                                                        tttATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTgggg                           >  1:935796/1‑100 (MQ=255)
                                                                        tttATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGCCGCCGGGGCGAAGCCGCGNCCNNATCCCGCCCCCGGCAAGCGCACGGGc                           >  1:3131852/1‑101 (MQ=18)
                                                                         ttATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCg                          >  1:3430313/1‑101 (MQ=255)
                                                                          tATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCgg                         >  2:3016347/1‑101 (MQ=255)
                                                                          tATCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGAGAAAACCCGGCCCCGGCAAGCGCCTGGGCgg                         >  1:1677172/1‑101 (MQ=21)
                                                                           aTCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCggg                        >  2:2481524/1‑101 (MQ=255)
                                                                            tcgtGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGa                       <  1:1265372/98‑1 (MQ=255)
                                                                            tCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGGAATCCCGGCGCCGGCAAGCGCCCggggcggg                       >  1:4441345/1‑96 (MQ=18)
                                                                            tCTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGa                       <  2:686423/101‑1 (MQ=255)
                                                                             cTTGCCCAAAATCCCCG.GGGGGCCGG..GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGaa                      <  1:3242489/101‑1 (MQ=25)
                                                                             cTTGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGaa                      >  1:815380/1‑101 (MQ=255)
                                                                              ttGCCCAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAg                     <  2:2778342/101‑1 (MQ=255)
                                                                                 cccAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATc                  <  2:636368/101‑1 (MQ=255)
                                                                                 cccAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGGAGATc                  >  1:1698226/1‑101 (MQ=25)
                                                                                  ccAAAATTGCCC.GGGGCGC.CGGGGGGGGGGG.CCCCGGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATc                  <  2:1994365/101‑1 (MQ=25)
                                                                                  ccAAAATATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcg                 >  2:4142350/1‑101 (MQ=255)
                                                                                   cAAAATATCCC.GGGGGTCGG..GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgc                <  2:3620258/101‑1 (MQ=255)
                                                                                     aaaTATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcgc              >  2:2543117/1‑101 (MQ=255)
                                                                                      aaTATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcgcg             >  2:793736/1‑101 (MQ=255)
                                                                                      aaTATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcgcg             >  2:2308717/1‑101 (MQ=255)
                                                                                      aaTATCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCCGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATcgcgcg             >  2:1885607/1‑101 (MQ=255)
                                                                                       atatCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGCAGATcgcgccc            >  2:704574/1‑99 (MQ=255)
                                                                                       atatCCC.GGGGGGC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGTGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCgcgcgc            <  2:3033079/101‑1 (MQ=25)
                                                                                        tatCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGcc           >  1:3690837/1‑101 (MQ=255)
                                                                                        tatCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCCCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGGAGATCGCGGGcc           >  1:4701518/1‑101 (MQ=25)
                                                                                          tCCC.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGcccc         >  1:311237/1‑101 (MQ=255)
                                                                                           ccc.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCg        >  2:180041/1‑101 (MQ=255)
                                                                                             c.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGa      >  1:2666616/1‑101 (MQ=255)
                                                                                             c.GGGGGTC.CG.GGGGCGGAG.CCCCCGGCGGGGCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGa      >  2:1516001/1‑101 (MQ=255)
                                                                                               gggggTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAg     >  1:973621/1‑101 (MQ=255)
                                                                                                 gggTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGCGCa   >  1:1897590/1‑101 (MQ=255)
                                                                                                  ggTC.CG.GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGGGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGGAGAGCGCGCGCCCCGGGGCaa  >  1:657720/1‑101 (MQ=25)
                                                                                                        |                                                                                                 
CGGCGTATTTGCACCAGGAAAAGCCAAAGCCGTCCCCGGCAGGACCGAAGGCCTCAGGACCCCGGGCCCC.TTTTATCTTGCCCAAAATATCCC.GGGGGTC.C..GGGGCGGAG.CCCCCGGCGGGTCGCCGGGGCGAAGCCGCGGCGAAATCCCGGCCCCGGCAAGCGCCTGGGCGGGAAGATCGCGCGCCCCGGAGCAA  >  NC_014034/2577183‑2577378

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 41 ≤  ATCG