Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,646,492 +C 3.7% intergenic (+450/+314) proS → / ← RCAP_rct00032 prolyl‑tRNA synthetase/tRNA‑Ala

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,646,4921.→C 3.7% 3.7 ‑0.6 ‑0.582intergenic (+450/+314)proS/RCAP_rct00032prolyl‑tRNA synthetase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (56/23):  total (59/23)
Fisher's exact test strand distribution p-value = 5.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.43e-01

Only 100 of 107 total aligned reads displayed.

GCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGGG  >  NC_014034/2646393‑2646592
                                                                                                    |                                                                                                    
ccAATCCCGATCCGCGACATCCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.c                                                                                                     <  2:748730/100‑1 (MQ=255)
 cAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTCG.cc                                                                                                    >  1:3901286/1‑101 (MQ=255)
  aaTCCCGATCCGCGACATCCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.ccc                                                                                                   <  2:4001594/101‑1 (MQ=255)
   aTCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGCCCCTG.cccc                                                                                                  >  1:2637854/1‑101 (MQ=255)
   aTCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.cccc                                                                                                  >  1:589890/1‑101 (MQ=255)
     cccGACCCGCGACATCCCCCAGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.cccccc                                                                                                <  1:2330946/101‑1 (MQ=255)
      ccGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.ccccccc                                                                                               >  2:3190599/1‑101 (MQ=255)
       cGACCCGCGACATCCCCCCCCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.cccccccc                                                                                              <  2:4215573/101‑1 (MQ=255)
        gATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.ccccccccc                                                                                             >  1:4412098/1‑101 (MQ=255)
         aTCCGCGACATACCCCCGCCCCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.cccccccccc                                                                                            <  2:2173150/101‑1 (MQ=255)
          tCCGCGACCTACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCa                                                                                           <  1:1440946/101‑1 (MQ=255)
          tCCGCGACATCCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCa                                                                                           <  2:2202370/101‑1 (MQ=255)
          tCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.ccccccccccc                                                                                           >  2:4649305/1‑100 (MQ=255)
            cgcgcccgccccccGCCGCCGGGATTGCGCGGGGGCCCCCCGGGCGAAAGCCGGGCGGGGGCGGCACAACCATGAAGCCCGGACCTTC.CCCCCCCCCCaga                                                                                         <  2:623848/92‑1 (MQ=255)
            cgcgACACCCCCCCGCCCCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCaga                                                                                         <  1:820606/101‑1 (MQ=255)
             gcgACACCCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCagag                                                                                        <  1:2183467/101‑1 (MQ=255)
             ccgccATCCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCagag                                                                                        <  1:2018544/97‑1 (MQ=255)
             cccaacaccccccCCCCCCCGGCTTGCCCCCGGCCCCCCGGGGCTCAGACGGGCCGGCGGCGGCACACCCATGACCCCCGAACCTTG.CCCCCCCCCCagag                                                                                        <  2:1601342/93‑1 (MQ=255)
              cgACATCCCCCCGCCCCCGGGTTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGt                                                                                       <  2:3932744/101‑1 (MQ=255)
              cgACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCcgagc                                                                                       >  1:2463710/1‑100 (MQ=255)
               gACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCcgaggc                                                                                      >  2:299826/1‑99 (MQ=255)
                 cATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCcgaggggc                                                                                    >  2:1970434/1‑97 (MQ=255)
                 cATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCcgacggcc                                                                                    >  2:1323086/1‑96 (MQ=255)
                  aTAGCCCCGCCGCCCGGCTTTCGCGCGGGACGCCCCGGCGCAAGCCGGGCGGCGGCGGGGCAACCATGAAGCCCCGACCCTT.cccccccccccacgagcgc                                                                                   >  2:4025610/1‑92 (MQ=25)
                  aTACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.ccccccccccccagggccc                                                                                   >  2:3859529/1‑92 (MQ=255)
                   tACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGCGCGCTcc                                                                                  >  2:1163768/1‑101 (MQ=255)
                    aCCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCCGAGTGCCccc                                                                                 >  1:4277845/1‑101 (MQ=255)
                      ccccGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCgc                                                                               >  2:4701427/1‑100 (MQ=255)
                           ccgccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCg                                                                          >  1:3692802/1‑101 (MQ=255)
                             gccCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGa                                                                        >  1:3174932/1‑101 (MQ=255)
                              cccGGCTTGCGCGCGGGACGCCCGGGCGGAAGCCGGGCGGCGGCGGCACCACCATGAAGCCGGGACCTTG.CCCCCCCCCCAGACACCCCCCGTCCCCGGAg                                                                       >  1:3947940/1‑101 (MQ=255)
                                   cTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGCCCCTG.CCCCCCCCCCAGCGCCCTCCCGCCACCGGAGGGGGc                                                                  >  1:1471289/1‑101 (MQ=255)
                                    ttGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGGGCTCCCGGCCCCGGGGGGGGCa                                                                 >  1:1799827/1‑101 (MQ=255)
                                     tGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCAt                                                                >  1:1791314/1‑101 (MQ=255)
                                         cgcgGGCCCCCGGGGGCCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGc                                                            <  2:1163234/101‑1 (MQ=255)
                                         cgcgGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGGGCTCCCGGCCCCGGAGGGGGCATCGGc                                                            >  2:3678066/1‑101 (MQ=255)
                                          gggggcggCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCAAGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCa                                                           <  1:2753368/94‑1 (MQ=255)
                                            gggCCGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAAc                                                         <  2:919667/101‑1 (MQ=255)
                                            gggACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGGGCTCCCGGCCCCGGGGGGGGGATCGGCAAc                                                         >  1:1358350/1‑101 (MQ=255)
                                                cccccGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACcgcg                                                     <  1:4374430/99‑1 (MQ=255)
                                                cGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTNCCGCCCCCGGAGGGGGCATCGGCAAcccac                                                     >  1:478928/1‑98 (MQ=255)
                                                 gCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCgcgc                                                    >  2:3969400/1‑101 (MQ=255)
                                                     gggCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGGGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCacg                                                >  1:997744/1‑101 (MQ=255)
                                                      ggCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCacga                                               >  1:4391471/1‑101 (MQ=255)
                                                      ggCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCacga                                               >  1:2455309/1‑101 (MQ=255)
                                                       gcggaggcggggCGGCGGCGGCACAACCATGAAGCCCGGGCCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCacgac                                              <  1:4211683/92‑1 (MQ=255)
                                                             gCCGGGGGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGacac                                        <  1:1081527/101‑1 (MQ=255)
                                                              ccGGGCGGCGGCGGCACAACCCGGAACCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACAcc                                       <  1:4574872/101‑1 (MQ=255)
                                                               cGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTGCCCCCCCCCCCAAAGTGCTCCCGGCCCCGGAGGGGGCATGGGCACACGCGCCACCACGGAGAcc                                       >  1:438020/1‑101 (MQ=255)
                                                               cGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACcg                                      >  2:2205243/1‑101 (MQ=255)
                                                               cGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcg                                      >  1:1491722/1‑101 (MQ=255)
                                                               cGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcg                                      >  1:1616714/1‑101 (MQ=255)
                                                                gggCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGCCACcgc                                     >  2:4033569/1‑101 (MQ=255)
                                                                gggCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACcgc                                     >  1:3128413/1‑101 (MQ=255)
                                                                  gcggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACCCCGCGa                                   >  2:4194012/1‑101 (MQ=255)
                                                                  gcggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGa                                   >  2:3366006/1‑101 (MQ=255)
                                                                   cggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGAt                                  >  2:361959/1‑101 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGAtt                                 >  1:964233/1‑101 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGAtt                                 <  2:538645/101‑1 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGAtt                                 <  1:2995020/101‑1 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGAtt                                 <  2:4510406/101‑1 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGAtt                                 >  1:3302456/1‑101 (MQ=255)
                                                                    ggcggcggcACAACCATGAAGCCCGGACCTTG..CCCCCCCCCAGAGGGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGGCGGACACCGCGATTg                                >  1:1067494/1‑101 (MQ=255)
                                                                     gcggcggcACAACCAAGAAGCCCGGCCCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTg                                <  2:4419753/101‑1 (MQ=255)
                                                                      cggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGa                               >  2:2108055/1‑101 (MQ=255)
                                                                       ggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGAt                              >  1:1165505/1‑101 (MQ=255)
                                                                       ggcggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCCCCACGCCGGACCCCGCGATTGAt                              >  2:3569206/1‑101 (MQ=255)
                                                                         cggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATcc                            <  1:4649561/101‑1 (MQ=255)
                                                                         cggcACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATcc                            <  2:2777538/101‑1 (MQ=255)
                                                                             acaACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGGGATTTATCCGccc                        >  1:976874/1‑101 (MQ=255)
                                                                             acaACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGccc                        >  1:3325138/1‑101 (MQ=255)
                                                                              caACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGcccc                       <  2:2119960/101‑1 (MQ=255)
                                                                              caACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGAAACCGCGATTGATCCGcccc                       >  2:1333065/1‑101 (MQ=255)
                                                                              caACCACGAAGCCCGGCCCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGcccc                       <  1:765746/101‑1 (MQ=255)
                                                                               aaCCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGGGGGGGCATCGGCAACCGCCCCACGCCGGACACCGCGATTGATCCGCCCCg                      >  1:29706/1‑101 (MQ=255)
                                                                               aaCCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGGGCTCCCGCCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATGGATCCCCCCCg                      >  1:2552299/1‑101 (MQ=255)
                                                                                 ccATGAAGCCCGGACCTTGCCCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCACCGGCAACCGCGCCCCGACGGACACCCGGATTGATCCGCCCCgg                     >  2:163859/1‑101 (MQ=255)
                                                                                 ccATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGGGGGGGCATCGGAACCCGCGCAACGACGGACACCCCGATTGATCCCCCCGGGa                    >  1:4706428/1‑101 (MQ=255)
                                                                                  cATGAAGCCCGGCCCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGAt                   <  2:4411848/101‑1 (MQ=255)
                                                                                  cATGAAGCCCGGACCTTG.CCCCCCCCCCCGAGGCCCCCCCGCCCCGGGAGGGGCACCGGCAAACCCGGCCCGAAGGACACCCCCGGTTAACCGCCCCGGAt                   >  1:1612332/1‑101 (MQ=255)
                                                                                   aTGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCCCGACGGACACCGCGATTGATCCGCCCCGGCTg                  >  1:4028695/1‑101 (MQ=255)
                                                                                   aTGAAGCCCGGACCTTG.CCCCCCCCCCAGAGGGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATg                  >  1:152247/1‑101 (MQ=255)
                                                                                      aaGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACa               >  1:309527/1‑101 (MQ=255)
                                                                                       aGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAg              <  2:2209246/101‑1 (MQ=255)
                                                                                        gCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAgg             >  1:1911837/1‑101 (MQ=255)
                                                                                         cccGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGaacgga            >  2:1962446/1‑96 (MQ=255)
                                                                                         cccGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAgga            >  2:2409528/1‑101 (MQ=255)
                                                                                          ctgtctcTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggac           <  1:934595/95‑1 (MQ=255)
                                                                                            ggACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacgg         >  1:2235520/1‑101 (MQ=255)
                                                                                             gACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAggacgga        >  2:3616177/1‑101 (MQ=255)
                                                                                               ccTTG.CCCCCCCGCCAGAGGGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGCTGACAGGACGGAcc      >  2:4700644/1‑101 (MQ=255)
                                                                                               ccTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGAcc      <  1:1163854/101‑1 (MQ=255)
                                                                                               ccTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCCCCCCGGATGCCAGGACGGAcc      >  1:2904099/1‑101 (MQ=255)
                                                                                                cTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCg     <  1:299860/101‑1 (MQ=255)
                                                                                                cTTG.CCCCCCCCCCAGAGGGCCCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACCCCGCGATTGATCCGCCCCGGATGACAggacaaatcg     >  2:934518/1‑95 (MQ=255)
                                                                                                 ttG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCgg    >  2:3977172/1‑101 (MQ=255)
                                                                                                  tG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCggg   >  2:3353065/1‑101 (MQ=255)
                                                                                                  tG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCggg   >  1:2470869/1‑101 (MQ=255)
                                                                                                   g.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGCCGGACGggga  >  2:620021/1‑100 (MQ=255)
                                                                                                   g.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCgggg  >  2:1931612/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCAATCCCGATCCGCGACATACCCCCGCCGCCCGGCTTGCGCGCGGGACGCCCGGGCGCAAGCCGGGCGGCGGCGGCACAACCATGAAGCCCGGACCTTG.CCCCCCCCCCAGAGTGCTCCCGGCCCCGGAGGGGGCATCGGCAACCGCGCCACGACGGACACCGCGATTGATCCGCCCCGGATGACAGGACGGACCGGGG  >  NC_014034/2646393‑2646592

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG