Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,678,900 +C 100% intergenic (+29/+58) RCAP_rcc02495 → / ← RCAP_rcc02496 GTPase, EngC family/group 1 glycosyl transferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,678,9001.→C100.0% 36.5 116intergenic (+29/+58)RCAP_rcc02495/RCAP_rcc02496GTPase, EngC family/group 1 glycosyl transferase
Reads supporting (aligned to +/- strand):  new base (65/38):  ref base (1/12):  total (66/50)
Fisher's exact test strand distribution p-value = 1.52e-04
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.20e-01

Only 100 of 150 total aligned reads displayed.

GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.CCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCGGA  >  NC_014034/2678800‑2678995
                                                                                                      |                                                                                                
gCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.Cg                                                                                                   >  2:2699171/1‑101 (MQ=255)
gCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.Cg                                                                                                   <  2:651655/101‑1 (MQ=255)
gCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGGGGGGGCAT.Cg                                                                                                   >  1:1415794/1‑101 (MQ=255)
gCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGCGGGGGCAT.Cg                                                                                                   >  1:985878/1‑101 (MQ=255)
   cgcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACTCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccc                                                                                               <  1:4227510/101‑1 (MQ=255)
   cgcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccc                                                                                               <  1:1420761/101‑1 (MQ=255)
   cgcgACCGCGCCTTCGGCAAGATGACCCGGGCGACGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccc                                                                                               <  1:4050205/101‑1 (MQ=255)
    gcgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.cccc                                                                                              >  2:1925801/1‑101 (MQ=255)
     cgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccccc                                                                                             >  1:147826/1‑100 (MQ=255)
     cgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccccc                                                                                             <  2:1411323/101‑2 (MQ=255)
     cgACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccccc                                                                                             >  1:499256/1‑100 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGGAT.CG.ccccca                                                                                            >  2:3224085/1‑99 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccccca                                                                                            <  1:2472833/101‑3 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccccca                                                                                            <  1:4654388/101‑3 (MQ=255)
      gACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.ccccca                                                                                            >  1:2363889/1‑99 (MQ=255)
         cgcgCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.cccccaccg                                                                                         >  1:3607856/1‑96 (MQ=255)
         cgcgCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.cccccaccg                                                                                         >  1:2317697/1‑96 (MQ=255)
          gcgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACcgc                                                                                        >  1:3816691/1‑101 (MQ=255)
          gcgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACcgc                                                                                        <  2:2379510/101‑1 (MQ=255)
          gcgcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTCGGGGCAT.CG.cccccacccc                                                                                        >  2:1269222/1‑95 (MQ=255)
            gcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCgcgc                                                                                      >  1:1877603/1‑101 (MQ=255)
            gcCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.CCCC.ACCGCGcc                                                                                     <  2:3398502/101‑1 (MQ=255)
                 cGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCCACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGAc                                                                                 >  1:3019016/1‑101 (MQ=255)
                    cAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGAcgcg                                                                              >  2:3981331/1‑101 (MQ=255)
                    cAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGAcgcg                                                                              >  2:2164301/1‑101 (MQ=255)
                    cAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGAcgcg                                                                              >  2:2737782/1‑101 (MQ=255)
                    cAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGAcgcg                                                                              >  1:1300528/1‑101 (MQ=255)
                     aaGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGGCTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGAcgcgc                                                                             >  1:1909941/1‑101 (MQ=255)
                         tGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCgcggcg                                                                         >  1:2867868/1‑101 (MQ=255)
                          gACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGcggcgg                                                                        >  2:1837732/1‑101 (MQ=255)
                           cccggggCGCCGCGGGGGGACAACCCCCGGCGGCGGGGCGCTGGGCGCGGGTCGGGGGGCGGGGGGGGGCAC.CGCCCCC.CCCGCGCCCGACGCGCGGCggg                                                                       <  1:982576/97‑1 (MQ=2)
                             ccGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCt                                                                     >  2:4187486/1‑101 (MQ=255)
                              cGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCtt                                                                    <  1:1203284/101‑1 (MQ=255)
                              cGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCtt                                                                    <  2:495223/101‑1 (MQ=255)
                               gggCGGCGCTGCGCGACAAACCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTg                                                                   <  2:4423005/101‑1 (MQ=255)
                                 gcggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTgcc                                                                 <  2:4211422/101‑1 (MQ=255)
                                   ggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTgcccg                                                               >  1:2351521/1‑101 (MQ=255)
                                   ggcgCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTgcccg                                                               >  2:632523/1‑101 (MQ=255)
                                       cTGCGCGACAAACCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAC.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCa                                                           <  1:4674551/101‑1 (MQ=37)
                                        ggcgcGAAAAACCCGGGCGGCGCGGCGACTGACGCGGTTTGGGGGGCGGGTGGGGGCATCCC.CCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCaa                                                          <  2:1649954/100‑1 (MQ=18)
                                           gcgACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga                                                       <  2:3748653/101‑1 (MQ=255)
                                           gcgACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAagga                                                       >  2:3060985/1‑101 (MQ=255)
                                            cgACAACCCCCGGCGGCGCGGCGACTGACGCGGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                                                      <  2:1325017/101‑1 (MQ=25)
                                            cgACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                                                      <  2:3746951/101‑1 (MQ=255)
                                            cgACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAaggag                                                      <  2:303440/101‑1 (MQ=255)
                                              aCAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAggg                                                    <  2:353546/101‑1 (MQ=255)
                                               cAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGCGGgc                                                   >  2:3673378/1‑101 (MQ=255)
                                               cAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc                                                   >  2:1616578/1‑101 (MQ=255)
                                               cAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgc                                                   >  1:1494041/1‑101 (MQ=255)
                                                aaaTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg                                                  >  2:3533230/1‑101 (MQ=255)
                                                aaaTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcg                                                  >  2:1181868/1‑101 (MQ=255)
                                                 aaTCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGgcgc                                                 >  1:4565015/1‑101 (MQ=255)
                                                    cccGGCTGCGCGGCGACTGCCGCCGGTTCGGGGGCCGGGGGGGGCAT.CGCCCCC.ACCGCGCCCGAGGCGCGGCGGGCCTGCCCGCCCAAGGAGGGAGgccc                                              >  2:1395533/1‑101 (MQ=25)
                                                     ccGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGccccc                                             <  1:2634220/101‑1 (MQ=255)
                                                     ccGGCGGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCATCCC.CCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGccccc                                             <  1:4415486/101‑1 (MQ=25)
                                                      ttgCTGCGCGGCGACTGACTCCGGTTCGGGGGCCGGGGGGGGAAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGGGGGCGCCCCCg                                            >  2:419775/3‑101 (MQ=25)
                                                      cGGGTGCGCGGCGACTGACGCCTGTTCGGGGGCCGGTGGGGGCAT.CG.CCCCGACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCg                                            <  2:2148747/101‑1 (MQ=25)
                                                       ggCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCCAGGAGGGCGCCCCCgg                                           >  2:132054/1‑101 (MQ=255)
                                                        gCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGa                                          >  2:3069654/1‑101 (MQ=255)
                                                         gtGCGCGGGGCCTGACGCCGGTTCGGGGGCGGGTGGGGGCCTCCC.CCCC.CCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAt                                         <  1:4313720/100‑1 (MQ=21)
                                                         cTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAt                                         <  2:3477732/101‑1 (MQ=255)
                                                         cTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAt                                         >  1:3648820/1‑101 (MQ=255)
                                                         cTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGACCGCCCAAGGAGGGCGCCCCCCGAt                                         >  2:612829/1‑101 (MQ=25)
                                                             gcggcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGAtctcc                                     >  1:312998/1‑100 (MQ=255)
                                                             gcggcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCa                                     <  2:3816456/101‑1 (MQ=255)
                                                             gcggcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCa                                     >  1:2979093/1‑101 (MQ=255)
                                                              cggcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAg                                    >  1:788566/1‑101 (MQ=255)
                                                               ggcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCCAGGAGGGCGCCCCCGGCTCTCAgc                                   >  1:4544106/1‑101 (MQ=25)
                                                               ggcgACTGACGCCGGTGCAGGGGAAGGTGGGGGCAA.CGCCCCC.ACGGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgc                                   <  1:3893529/101‑1 (MQ=18)
                                                                gcgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGGTCTCAgcg                                  >  2:4484765/1‑101 (MQ=255)
                                                                 cgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgcgc                                 >  1:9649/1‑101 (MQ=255)
                                                                 cgACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAgcgc                                 >  1:3775377/1‑101 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                                <  2:4598356/101‑1 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                                >  1:1610098/1‑101 (MQ=255)
                                                                  gACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.CCCCAACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCa                                <  1:1565616/101‑1 (MQ=255)
                                                                   aCTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCaa                               >  2:4544805/1‑101 (MQ=255)
                                                                    cTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAAc                              <  1:544663/101‑1 (MQ=255)
                                                                     tGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACg                             >  1:627062/1‑101 (MQ=255)
                                                                     tGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCCTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACg                             >  2:4661310/1‑101 (MQ=255)
                                                                       aCGCCGGTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                           <  2:3072888/101‑1 (MQ=255)
                                                                       aCGCCGGTGCGGGGGCGGGGGGGGGCATCCC.CCCC.CCCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTg                           <  1:1804892/101‑1 (MQ=21)
                                                                         gCCGTTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgcc                         <  2:3702713/101‑1 (MQ=255)
                                                                            ggTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgcc                      >  1:3947730/1‑101 (MQ=255)
                                                                            ggTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTgccgcc                      >  2:4157875/1‑101 (MQ=255)
                                                                             ggtCGGGGGCCGGTGGGGACAC.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGTCCCCGGATCTCAGCGCAACGTGccgccc                     <  2:1550631/99‑1 (MQ=25)
                                                                             gTTCGGGGGCCGGTGGGGGCAT.CGCCCCC.ACAGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCCAAGTGccgccc                     >  2:3236431/1‑101 (MQ=21)
                                                                              ttCGGGGGCCGGGGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgcccg                    <  1:3040749/101‑1 (MQ=255)
                                                                              tgggggggGCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGccgcccg                    <  1:1837844/98‑1 (MQ=25)
                                                                                cGGGGGCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCcgcccgcc                  >  2:4391846/1‑101 (MQ=255)
                                                                                  ggggCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCccgccgc                >  2:867572/1‑100 (MQ=255)
                                                                                  ggggCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCCCCGt                >  1:1951821/1‑101 (MQ=255)
                                                                                   gggCCGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCAGCCCGCCGtc               >  1:816828/1‑101 (MQ=255)
                                                                                      gcGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCg            <  2:2351388/100‑1 (MQ=255)
                                                                                      cgggTGGGGGCATCCC.CCCC.GCGGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCg            <  2:1556891/99‑1 (MQ=21)
                                                                                      ccGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCg            <  1:2053874/101‑1 (MQ=255)
                                                                                      ccGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCg            >  1:955329/1‑101 (MQ=255)
                                                                                      ccGGGGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCg            <  1:647993/101‑1 (MQ=255)
                                                                                       cGGTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGa           >  2:4355821/1‑101 (MQ=255)
                                                                                         gTGGGGGCAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACa         <  1:2143667/101‑1 (MQ=255)
                                                                                                cAT.CGCCCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGCCTCGACACAGCGGa  >  1:1983056/1‑101 (MQ=37)
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GCCCGCGACCGCGCCTTCGGCAAGATGACCCGGGCGGCGCTGCGCGACAAATCCCGGCTGCGCGGCGACTGACGCCGGTTCGGGGGCCGGTGGGGGCAT.CG.CCCC.ACCGCGCCCGACGCGCGGCGGGCTTGCCCGCCCAAGGAGGGCGCCCCCGGATCTCAGCGCAACGTGCCGCCCGCCGTCTCGACAAAGCGGA  >  NC_014034/2678800‑2678995

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG