Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,849	+G	100%	intergenic (‑181/+112)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,849	1	.→G	100.0%	66.3	21	intergenic (‑181/+112)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (1/20):  ref base (0/0):  total (1/20)

GTTCGGGCGGAATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943                                                                                                        |                                                                                                gtcggggGGGAAGAGCAACCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc                                                                                                  <  1:161500/97‑1 (MQ=255)  ttggggCGGAATAGGAACCGCGCCCCGCTTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.g                                                                                                <  1:4751240/98‑1 (MQ=255)  tgcGGGGGGAATAGGAAACGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.g                                                                                                <  1:1925441/99‑1 (MQ=255)  ggcGGGCGAAATAGGAAACGCGCCCCGCTTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.g                                                                                                <  1:942343/99‑1 (MQ=255)    ggggGGGAAGAGAAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggc                                                                                              <  1:2622668/100‑3 (MQ=255)    ggggGGGAAAAGGAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggc                                                                                              <  1:1009460/100‑3 (MQ=38)    cGGGCGGAATAGGAAACGCGCCCCCCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggc                                                                                              <  1:1737468/101‑3 (MQ=255)     gggCGAAATAGGAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcg                                                                                             <  2:1967643/101‑4 (MQ=38)     gggCGAAAAAGGAACCGCGCCCCGGTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcg                                                                                             <  2:4086403/101‑4 (MQ=37)       gCGGAATAGAACACCCGACCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACGGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcggg                                                                                           <  2:1208061/101‑6 (MQ=37)         ggAATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccgg.ccc.ggcgggcg                                                                                         >  1:3535627/1‑94 (MQ=255)            aTAGGAACCGCGCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCgaga                                                                                       <  2:4195622/101‑1 (MQ=25)             tAGGAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                      <  1:493177/101‑1 (MQ=37)             tAGGAACCGCGACCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                      <  2:1951242/101‑1 (MQ=37)             tAGGAAACGCGACACGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                      >  2:2155094/1‑101 (MQ=37)             tAGAAACCGCGCCCCGCTTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                      <  1:4555752/101‑1 (MQ=25)             gaGGAAGCGCCCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                      <  1:3838747/100‑1 (MQ=25)             agggAACCGCGCCCCGGGTGC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGaa                                                                                      <  2:3344831/99‑1 (MQ=25)              aGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAg                                                                                     <  2:2369916/101‑1 (MQ=255)               ggAAACGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAgc                                                                                    <  2:3045285/101‑1 (MQ=37)                   aCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                <  1:2061877/101‑1 (MQ=25)                   aCGCGACCCGGGTTG.CGCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                <  1:4445886/101‑1 (MQ=25)                   aCGAGACCCGCGTTC.CGCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCTCCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgccc                                                                                <  2:1317268/101‑1 (MQ=25)                    cgcgCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccg                                                                               <  1:2721320/101‑1 (MQ=25)                    cgcgACCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccg                                                                               <  2:2855843/101‑1 (MQ=25)                      cgccccGGGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGcgcccgcc                                                                             <  1:3931988/98‑1 (MQ=21)                         ccccgtttccccccGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  1:1071047/91‑1 (MQ=21)                          ccgttttt.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.ggtc                                                                        <  2:2520257/96‑3 (MQ=25)                           ccgcttccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggc                                                                        <  2:3535408/93‑3 (MQ=21)                             tcttcgccccGCCCTAAGCGCCGGGTTGGGGCATGCCCGCTCCACCCCGCACATCAGGGGGCAGCGCCCGG.CCCGGCGGGCGAAAGGCGCCCggc.gggccg                                                                      <  2:192946/95‑5 (MQ=9)                             ggttccccccGCCGGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCAGCGCGCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                      <  2:837940/95‑5 (MQ=21)                             gcttccccccGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCCCCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                      <  1:131274/95‑5 (MQ=21)                             cgTTTCCCCCGCCCGCAGCGCCCGGTGGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCGCCCgcc.gggccg                                                                      <  1:1474179/101‑5 (MQ=21)                                                                       cAGCCCGCACCACAGAGGGCAGCGCCCGG.CCCGGCGGGCGAGAAGCCCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGCGGTCACAGGAc                             >  1:2035637/1‑101 (MQ=17)                                                                                       gggCACCGCCCGG.CCCGGCGGGCGAGACGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGc             <  2:359905/101‑1 (MQ=17)                                                                                         gcggcccccggcccc.GCGGCCGAGACGCCGCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCa           <  2:503121/95‑1 (MQ=18)                                                                                                ccgg.cccgGCGGGGGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGAt    <  2:2035516/101‑1 (MQ=18)                                                                                                 cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  2:3401351/1‑101 (MQ=18)                                                                                                 cgg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATg   >  1:2063779/1‑101 (MQ=18)                                                                                                  gg.cccgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  2:975865/1‑101 (MQ=18)                                                                                                        |                                                                                                GTTCGGGCGGAATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGG.CCC.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG