Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,808,557	+G	100%	intergenic (+112/+42)	pyrD2 → / ← acsA2	dihydroorotate oxidase/acetate‑‑CoA ligase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,808,557	1	.→G	100.0%	112.1	55	intergenic (+112/+42)	pyrD2/acsA2	dihydroorotate oxidase/acetate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  new base (34/21):  ref base (0/0):  total (34/21)

CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCCTCCGGTCAGGCCCGGTTCATCCGGTTGTCGATCAGCTCCTGCACCACGCCCGGATCG  >  NC_014034/2808457‑2808650                                                                                                      |                                                                                              cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGgaga                                                                                                >  2:68812/1‑101 (MQ=38) cTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGgaga                                                                                                <  2:2800327/101‑1 (MQ=38)  tGCGGGGGGTTCACACCCCCCCCCCCCCCTGGGATAATTTCGCCAAGGAAAAACACAGACTTTTGCCCGGTCACCCTTGCGCAAAACTACCCGGGGGgga.g                                                                                              >  2:1220681/1‑101 (MQ=2)    cGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggt                                                                                            <  1:3666135/101‑3 (MQ=18)     gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtg                                                                                           >  1:3978881/1‑98 (MQ=17)     gggggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtg                                                                                           >  2:1899895/1‑98 (MQ=17)       gggTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGaga.ggtggc                                                                                         >  1:3917092/1‑96 (MQ=14)         gTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGGGA.GGGgggcc                                                                                       >  2:2154638/1‑100 (MQ=255)          tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                                                                                       <  1:975990/101‑1 (MQ=2)          tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                                                                                       <  2:3721011/101‑1 (MQ=2)          tttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCa                                                                                       >  2:3076365/1‑101 (MQ=9)           ttCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAg                                                                                      <  2:3380663/101‑1 (MQ=9)            tCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAgg                                                                                     <  2:1399717/101‑1 (MQ=12)             cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGc                                                                                    >  1:941536/1‑101 (MQ=16)             cacaCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGc                                                                                    >  1:1008660/1‑101 (MQ=16)              acacCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCa                                                                                   >  2:1365958/1‑101 (MQ=16)               cacccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCaa                                                                                  <  2:1309982/101‑1 (MQ=12)               cacccccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCaa                                                                                  <  1:1558118/101‑1 (MQ=12)                   cccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCg                                                                              <  2:169562/101‑1 (MQ=12)                    ccgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgg                                                                             <  2:4615002/101‑1 (MQ=12)                     cgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggg                                                                            >  1:1997153/1‑101 (MQ=16)                     cgcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggg                                                                            <  1:4121631/101‑1 (MQ=12)                      gcacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggg                                                                           <  1:1804256/101‑1 (MQ=12)                       cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggggg                                                                          <  1:2305912/101‑1 (MQ=12)                       cacccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCggggg                                                                          >  1:1088266/1‑101 (MQ=19)                        acccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCgggggg                                                                         >  1:2859207/1‑101 (MQ=19)                          ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                       <  2:98630/101‑1 (MQ=16)                          ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                       >  2:1081206/1‑101 (MQ=16)                          ccccgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGggcg                                                                       >  1:2045710/1‑101 (MQ=16)                             cgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGgcggcg                                                                    >  2:4592413/1‑101 (MQ=16)                             cgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGgcggcg                                                                    <  1:312008/101‑1 (MQ=12)                             cgTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGCCGGGGGgcggcg                                                                    >  2:889661/1‑101 (MQ=255)                              gTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGgcgc                                                                   <  1:4397223/101‑1 (MQ=16)                               tGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcc                                                                  <  2:4504497/101‑1 (MQ=12)                                ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCANGTCGGGGGGCGGCGccc                                                                 >  2:810114/1‑101 (MQ=16)                                ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc                                                                 >  1:3937767/1‑101 (MQ=16)                                ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc                                                                 >  1:3592172/1‑101 (MQ=16)                                ggCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccc                                                                 >  1:2804153/1‑101 (MQ=16)                                 gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGGGcccc                                                                >  1:642981/1‑101 (MQ=255)                                 gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGGGcccc                                                                >  1:998057/1‑101 (MQ=255)                                 gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccc                                                                <  1:1900689/101‑1 (MQ=12)                                 gCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccc                                                                <  2:165418/101‑1 (MQ=12)                                  cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGGGccccc                                                               >  1:76127/1‑101 (MQ=255)                                  cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                               >  1:1489164/1‑101 (MQ=16)                                  cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                               <  1:3340228/101‑1 (MQ=12)                                  cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                               >  1:2005130/1‑101 (MQ=16)                                  cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGccccc                                                               >  1:2592919/1‑101 (MQ=16)                                  cGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGCCGGCGccccc                                                               >  1:2319713/1‑101 (MQ=255)                                   gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGgccccc                                                              >  2:3126308/1‑101 (MQ=255)                                   gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc                                                              >  2:2743092/1‑101 (MQ=12)                                   gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc                                                              >  1:3393167/1‑101 (MQ=12)                                   gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc                                                              <  2:3392961/101‑1 (MQ=12)                                   gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc                                                              <  2:1637771/101‑1 (MQ=12)                                   gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGcccccc                                                              >  2:1401560/1‑101 (MQ=12)                                   gTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGGGGCGCAGGGAAGTCGGGGGGCGGCGcccccc                                                              >  2:1505137/1‑101 (MQ=11)                                    tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                             >  1:3003266/1‑101 (MQ=12)                                    tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                             >  1:512704/1‑101 (MQ=12)                                    tATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCg                                                             >  2:585597/1‑101 (MQ=12)                                     aTTTGCGCGAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc                                                            >  2:2105678/1‑101 (MQ=9)                                     aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc                                                            >  2:3049201/1‑101 (MQ=9)                                     aTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGc                                                            >  2:340909/1‑101 (MQ=9)                                      tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGcc                                                           >  1:483831/1‑101 (MQ=2)                                      tttGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGAGGTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGcc                                                           <  2:1499526/101‑1 (MQ=2)                                                                                      cccccggggggggGGG.GGGGCGCAGGCAGGGGGGGGGGCGGCCCCCCCCGCCTCCGGTCAGGCCCGGTTCATCCGGTTGTCGATCAGCTCCTGCACCACGc          <  1:1804940/94‑1 (MQ=11)                                                                                              gggggGGG.GTGGCGCACGCAAGTCGCGGCGCGGCCCCCCCCGCCTCCGGTCAGGCCCGGTTCATCCGGTTCTCGATCAGCTCCTGCACCACGCCCGGATCg  <  2:941459/101‑1 (MQ=11)                                                                                                      |                                                                                              CTGCGGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCGCAAATCTCCCGGGGGGAGA.GTGGCGCAGGCAAGTCGGGGGGCGGCGCCCCCCGCCTCCGGTCAGGCCCGGTTCATCCGGTTGTCGATCAGCTCCTGCACCACGCCCGGATCG  >  NC_014034/2808457‑2808650 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG