Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,880,582	+G	100%	coding (1003/1053 nt)	pdxA ←	4‑hydroxythreonine‑4‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,880,582	1	.→G	100.0%	25.6	20	coding (1003/1053 nt)	pdxA	4‑hydroxythreonine‑4‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (19/0):  ref base (0/0):  total (20/0)

GGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........C..CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGC  >  NC_014034/2880488‑2880635                                                                                                                   |                                                         ggcggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcac                                                   >  1:2053843/1‑97 (MQ=255) ggcggcAGCCCGTCAACCGCACCCAAAGCCCGCCCCCTTCCTTTTCATCTTTCTTCAAATATCCGGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcac                                                   <  2:2985499/101‑5 (MQ=25)  gcggcAGCCCTTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATACCCGGGGGGTCCGGGGGCAGACCCCCCGGCG..........GC.........g..cgtcaca                                                  <  2:4514289/101‑6 (MQ=25)  ctggcCGCCCCTCACCCGCAGCGAAGGCCCGCCCCCTACCTTTTCATCTTGCTTCAAATTTCCGGGGGGTCCGGGGGGAGAACCCCCGGCG..........GC.........g..cgtcaca                                                  <  2:3098333/99‑6 (MQ=255)   cggcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCCCCG..........GC.........g..cgtcacag                                                 >  2:4207800/1‑95 (MQ=25)     gcAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagcc                                               >  1:1997346/1‑93 (MQ=255)      cAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........g..cgtcacagccg                                              >  1:4159063/1‑92 (MQ=25)                                          ttttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCcgc                   >  2:4501785/1‑101 (MQ=2)                                           tttCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                  >  1:1037891/1‑101 (MQ=2)                                           tttCATCTTGCTTCAAATATCCCGGGGGCCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCcgcg                  >  2:527606/1‑101 (MQ=2)                                             tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                >  2:123905/1‑101 (MQ=9)                                             tCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGAt                >  2:3495523/1‑101 (MQ=9)                                                tCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAg             >  1:4722093/1‑101 (MQ=9)                                                  ttGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGGGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCCCCGCCATCGTCAAGGCCGCGATCCGAc           >  2:2821630/1‑101 (MQ=9)                                                  ttGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCCTCTTCGCCGCCCTCGTCAAGGCCGCGATCAGAc           >  1:2382809/1‑101 (MQ=9)                                                   tGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACt          >  2:4634201/1‑101 (MQ=9)                                                   tGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACt          >  2:3135307/1‑101 (MQ=9)                                                     cTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACtgt        >  1:547718/1‑101 (MQ=12)                                                     cTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGCCAAGGCCGCGATCAGACtga        >  1:1236281/1‑100 (MQ=9)                                                      ttCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGatcaga       >  2:2840661/1‑96 (MQ=9)                                                       tCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGc      >  1:3379932/1‑101 (MQ=12)                                                       tCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCACCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGc      >  2:4729504/1‑101 (MQ=9)                                                         aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  2:2475196/1‑101 (MQ=12)                                                         aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCgg    >  2:3956869/1‑101 (MQ=12)                                                         aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGGCACAGC.........CG.CGC..CGCCCCCCTCTTCGCCGCCATCGTCAAGGCCGCCATCAAACTGTGCgg    >  2:3251469/1‑101 (MQ=2)                                                         aaaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCGCGGCACAGC..CGCGGCGGCCCCATCTTCGCCGCCCTCGTCAAGGCCGCGCTCAGACTGCGCgg    >  2:129286/1‑101 (MQ=2)                                                          aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  1:3887630/1‑101 (MQ=12)                                                          aaTATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCGGCGCGTCACAGC.........CG.CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCggg   >  2:1398853/1‑101 (MQ=12)                                                           atatCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GCGCGTCACAGCCGCGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGc  >  1:1326942/1‑101 (MQ=12)                                                                                                                   |                                                         GGCGGCAGCCCGTCAATCGCAGCCAAAGCCCGTCCCCTTCCTTTTCATCTTGCTTCAAATATCCCGGGGGTCCGGGGGCAGAGCCCCCGGCG..........GC.........C..CGC..CGCCCCCATCTTCGCCGCCATCGTCAAGGCCGCGATCAGACTGTGCGGGC  >  NC_014034/2880488‑2880635 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 22 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG