Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,925,524 +G 100% intergenic (+308/‑127) RCAP_rcc02735 → / → RCAP_rcc02736 virulence‑associated protein E/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,925,5241.→G100.0% 1116.5 298intergenic (+308/‑127)RCAP_rcc02735/RCAP_rcc02736virulence‑associated protein E/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (156/142):  ref base (0/0):  total (156/142)

Only 100 of 329 total aligned reads displayed.

ATTCTTCTTTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAG  >  NC_014034/2925424‑2925618
                                                                                                     |                                                                                               
aTTCTTCTTTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGaa                                                                                                  <  2:262581/101‑1 (MQ=255)
 ttcttcTTTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCCCTTGAGCGGTGaag                                                                                                 >  2:1102458/1‑100 (MQ=255)
   cttcttTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGaagtg                                                                                               >  1:1341481/1‑98 (MQ=255)
    ttcttTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTTAGCGGTGaagtgg                                                                                              >  1:1035825/1‑97 (MQ=255)
     tcttTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGAGAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGaagtggg                                                                                             >  2:2128001/1‑96 (MQ=255)
      cttTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGa                                                                                            >  1:3099391/1‑101 (MQ=255)
      cttTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGa                                                                                            >  1:682151/1‑101 (MQ=255)
       tttATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGAc                                                                                           >  1:1533204/1‑101 (MQ=255)
       tttATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGAc                                                                                           <  1:4641506/101‑1 (MQ=255)
        ttATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACa                                                                                          <  1:3453381/101‑1 (MQ=255)
        ttATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACa                                                                                          >  2:3873938/1‑101 (MQ=255)
         tATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAg                                                                                         <  2:582168/101‑1 (MQ=255)
          aTGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGc                                                                                        >  1:3920094/1‑101 (MQ=255)
          aTGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGc                                                                                        <  2:1164843/101‑1 (MQ=255)
            gCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGATCTCGCACTTGAGCGGGGAAGTGGGAAAGccc                                                                                      >  2:3904336/1‑101 (MQ=255)
            gCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGccc                                                                                      >  1:3120825/1‑101 (MQ=255)
              gACGTAGGGAAAAAGGTGGGAGAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCaa                                                                                    >  1:2703340/1‑101 (MQ=255)
              gACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCaa                                                                                    <  2:4162756/101‑1 (MQ=255)
                cGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAg                                                                                  <  1:3241329/101‑1 (MQ=255)
                  tAGGGAAAAAGGAGGGACAAGTGGGACAGGTGAGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCg                                                                                >  1:2581055/1‑101 (MQ=255)
                   aGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGa                                                                               >  1:481662/1‑101 (MQ=255)
                   aGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGa                                                                               >  2:2047697/1‑101 (MQ=255)
                     ggAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAgg                                                                             >  1:1446598/1‑101 (MQ=255)
                       aaaaaGGTGGGATAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTg                                                                           <  1:1767164/101‑1 (MQ=255)
                        aaaaGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTgg                                                                          <  2:2354005/101‑1 (MQ=255)
                         gatcttGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTggg                                                                         <  1:225249/96‑1 (MQ=255)
                         aaaGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTggg                                                                         >  2:4350009/1‑101 (MQ=255)
                          aaGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGa                                                                        <  1:4354694/101‑1 (MQ=255)
                              tGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGAGAtc                                                                    >  2:225224/1‑100 (MQ=255)
                              tGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATg                                                                    <  2:3405536/101‑1 (MQ=255)
                               gggACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATgg                                                                   <  2:2211366/101‑1 (MQ=255)
                               gggACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATgg                                                                   <  2:2387207/101‑1 (MQ=255)
                               gggACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACCTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATgg                                                                   <  1:4018364/101‑1 (MQ=255)
                                 gACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGAt                                                                 >  2:536239/1‑101 (MQ=255)
                                  aCAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATg                                                                <  2:1355801/101‑1 (MQ=255)
                                   cAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGc                                                               >  2:2742604/1‑101 (MQ=255)
                                    aaGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCg                                                              >  2:316168/1‑101 (MQ=255)
                                       tGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCgggc                                                           >  1:3283955/1‑99 (MQ=255)
                                        gggACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCg                                                          >  2:84982/1‑101 (MQ=255)
                                        gggACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCg                                                          >  1:2194044/1‑101 (MQ=255)
                                          gACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCggg                                                        >  1:541217/1‑101 (MQ=255)
                                             aGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGc                                                     >  1:2421086/1‑101 (MQ=255)
                                             aGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGGGGGACATGGATGCGGTCGGGCGc                                                     >  1:1069160/1‑101 (MQ=255)
                                                tGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                  <  2:282783/101‑1 (MQ=255)
                                                tGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtt                                                  <  2:770405/101‑1 (MQ=255)
                                                  ggACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtttt                                                <  1:2524779/101‑1 (MQ=255)
                                                  ggACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtttt                                                <  2:3431619/101‑1 (MQ=255)
                                                      aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                            <  1:4418537/101‑1 (MQ=255)
                                                      aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                            <  2:3120625/101‑1 (MQ=255)
                                                      aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                            <  1:416217/101‑1 (MQ=255)
                                                      aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                            <  2:1483836/101‑1 (MQ=255)
                                                       aGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.g                                          >  1:2982949/1‑101 (MQ=255)
                                                       aGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.g                                          <  2:2595561/101‑1 (MQ=255)
                                                        gCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.Ga                                         >  1:2426484/1‑101 (MQ=255)
                                                        gCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.Ga                                         <  1:1839524/101‑1 (MQ=255)
                                                         cATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GAt                                        >  2:1309801/1‑101 (MQ=255)
                                                         cATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GAt                                        >  2:2902975/1‑101 (MQ=255)
                                                         cATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GAt                                        >  2:1754062/1‑101 (MQ=255)
                                                          aTTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGGGGTGAAGTGGGACAGCCCAAAGCGAGGGGGGACNTGGATGCGGTCGGGCGCATTTTCCTT.GATc                                       >  1:1138671/1‑101 (MQ=255)
                                                           ttGTTCTGAAAGGAAAACTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCg                                      <  2:91577/101‑1 (MQ=255)
                                                            tGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGa                                     >  1:4544443/1‑101 (MQ=255)
                                                             gTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCNCATTTTCCTT.GATCGAg                                    >  2:49620/1‑101 (MQ=255)
                                                               tCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTg                                  >  1:3748109/1‑101 (MQ=255)
                                                                 tGAAAGGAAAATTTGCCGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGcc                                <  2:4582886/101‑1 (MQ=255)
                                                                 tGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGCGTGca                                >  2:3048319/1‑100 (MQ=255)
                                                                  gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTAGATCggaagag                                >  1:2916240/1‑95 (MQ=255)
                                                                  gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCt                               <  1:4262209/101‑1 (MQ=255)
                                                                   aaaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTg                              >  2:3353322/1‑101 (MQ=255)
                                                                   aaaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTg                              >  2:1948735/1‑101 (MQ=255)
                                                                     aGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCa                            <  1:2774152/101‑1 (MQ=255)
                                                                       gAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAAc                          <  2:3781364/101‑1 (MQ=255)
                                                                       gAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAAc                          <  2:3731325/101‑1 (MQ=255)
                                                                       gAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAAc                          <  1:3903897/101‑1 (MQ=255)
                                                                        aaaaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACg                         >  1:125660/1‑101 (MQ=255)
                                                                         aaaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGt                        >  2:3614086/1‑101 (MQ=255)
                                                                           aTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGa                      >  1:42993/1‑101 (MQ=255)
                                                                           aTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGGGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGa                      >  1:1911026/1‑101 (MQ=255)
                                                                               gACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTcc                  >  1:1087816/1‑101 (MQ=255)
                                                                                 cGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCt                <  1:1574930/101‑1 (MQ=255)
                                                                                 cGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCt                <  2:4160731/101‑1 (MQ=255)
                                                                                 cGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCt                <  2:4484026/101‑1 (MQ=255)
                                                                                  gTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTg               <  1:844698/101‑1 (MQ=255)
                                                                                  gTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTg               >  2:4098712/1‑101 (MQ=255)
                                                                                   gcccCCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGGGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGc              <  2:1138585/100‑1 (MQ=255)
                                                                                    ctcACCTGAGCGGTGAAGTGGGACAGCCCAAAGGGAGGTGGGACATGGATGCGGAGGGGGGAAATATCCTT.GGTCGAGGGCCTGAAAAGTGAtaacatgcg             >  2:3287722/3‑93 (MQ=255)
                                                                                    cccACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCt             >  1:3064753/1‑101 (MQ=255)
                                                                                       acatGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCAt          <  2:3928909/98‑1 (MQ=255)
                                                                                       aCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCAt          <  2:3526955/101‑1 (MQ=255)
                                                                                       aCTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCAt          <  2:3031101/101‑1 (MQ=255)
                                                                                        cTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATg         >  2:1569114/1‑101 (MQ=255)
                                                                                          tGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGGCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCCCATGAc       >  1:770302/1‑101 (MQ=255)
                                                                                           gAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAcc      >  2:1450680/1‑101 (MQ=255)
                                                                                            aGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCa     >  1:3813241/1‑101 (MQ=255)
                                                                                              cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa   >  1:98613/1‑101 (MQ=255)
                                                                                              cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa   <  1:3614305/101‑1 (MQ=255)
                                                                                              cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa   >  2:698862/1‑101 (MQ=255)
                                                                                              cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa   <  2:3235866/101‑1 (MQ=255)
                                                                                              cGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGCATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaaa   <  1:3305316/101‑1 (MQ=255)
                                                                                               ggTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAg  >  2:1668727/1‑101 (MQ=255)
                                                                                               ggTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTT.GATCGAGTGCCTGCAAAGTGATTCCCTGCTCATGACCAAAg  <  1:4457147/101‑1 (MQ=255)
                                                                                                     |                                                                                               
ATTCTTCTTTATGCGACGTAGGGAAAAAGGTGGGACAAGTGGGACAGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTT.GATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAG  >  NC_014034/2925424‑2925618

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG