Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,173,548 +C 100% intergenic (+583/‑99) tyrA → / → RCAP_rcc02983 prephenate dehydrogenase/extensin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,173,5481.→C100.0% 301.4 79intergenic (+583/‑99)tyrA/RCAP_rcc02983prephenate dehydrogenase/extensin family protein
Reads supporting (aligned to +/- strand):  new base (53/26):  ref base (0/0):  total (53/26)

CCCCCGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGG.CCGCCGCGCAAGCGC.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173448‑3173642
                                                                                                     |                                                                                                 
cccccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCgg                                                                                                    >  1:3864662/1‑101 (MQ=255)
cccccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCgg                                                                                                    <  2:2237666/101‑1 (MQ=255)
 ccccGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGg.c                                                                                                  >  1:2109227/1‑101 (MQ=255)
    cGGTCGCCTCGGCCGCCCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGg.cccg                                                                                               <  1:2278937/101‑3 (MQ=255)
     ggTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGg.cccgc                                                                                              >  2:3241963/1‑98 (MQ=255)
     ggTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGg.ccccc                                                                                              >  2:3385149/1‑101 (MQ=255)
      gTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGg.cccgcc                                                                                             >  1:1086573/1‑97 (MQ=255)
      gTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGg.cccgcc                                                                                             >  2:2971936/1‑97 (MQ=255)
            tCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaa                                                                                        <  1:4522987/101‑1 (MQ=255)
            ccgcccGCGCCGGTCCCGCCCCCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaa                                                                                        <  2:4103966/97‑1 (MQ=255)
             cGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaag                                                                                       >  2:4426317/1‑101 (MQ=255)
                ccGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc                                                                                    <  1:3797387/101‑1 (MQ=255)
                 cgcgCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCCCCgcgcaagcgc.a                                                                                  >  1:4719103/1‑101 (MQ=255)
                  gcgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.a                                                                                <  2:2848550/101‑1 (MQ=255)
                   cgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.ac                                                                               <  2:959748/101‑2 (MQ=255)
                   cgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.ac                                                                               <  1:1364602/101‑2 (MQ=255)
                      cGGTCCCGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgcc                                                                            <  2:3864420/101‑5 (MQ=255)
                       ggTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgccc                                                                           >  1:3637495/1‑96 (MQ=255)
                       ggTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgccc                                                                           >  2:3834021/1‑96 (MQ=255)
                       ggTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgccc                                                                           >  2:3452422/1‑96 (MQ=255)
                         tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGccccc                                                                         <  2:3217316/101‑1 (MQ=255)
                            cccccccccccgccCCCCCCACCCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCccccgcc                                                                      <  2:431357/99‑1 (MQ=255)
                             gcccgcccCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCccccgccc                                                                     <  2:1114759/101‑1 (MQ=255)
                             gcccgcccCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCCCGCAAGCGC.A.ACGCccccgccc                                                                     >  2:3074118/1‑101 (MQ=255)
                              cccccccccgccCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCccccgcccc                                                                    <  2:2109106/101‑1 (MQ=255)
                                cgcccccgccccCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCgg                                                                  >  2:299664/1‑101 (MQ=255)
                                 ccccccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGa                                                                 <  2:1933653/100‑1 (MQ=255)
                                    cccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgc                                                              <  1:1750719/101‑1 (MQ=255)
                                      cgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgc                                                            >  1:3061630/1‑101 (MQ=255)
                                       gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCCAGCGC.A.ACGCCCCCGCCCCGGAAgcgcc                                                           >  1:538309/1‑101 (MQ=255)
                                       gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.ACC.GCCCCCGCCCCGGAAgcccc                                                           >  2:2315211/1‑101 (MQ=255)
                                        cccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgccc                                                          >  1:4639062/1‑101 (MQ=255)
                                         ccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgcccg                                                         >  2:3742330/1‑101 (MQ=255)
                                              ccacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgcccgcagcc                                                    >  1:610319/1‑97 (MQ=255)
                                               cccccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                   <  2:3231587/97‑1 (MQ=255)
                                               cacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                   <  1:3834256/101‑1 (MQ=255)
                                                acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                  >  1:3115922/1‑101 (MQ=255)
                                                 cTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGac                                                 >  1:1050064/1‑100 (MQ=255)
                                                  ccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                <  1:3074306/100‑1 (MQ=255)
                                                   ccggCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGCAGCGCCCGCGCCCGAAGc                                               >  1:2709548/4‑101 (MQ=255)
                                                   cccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.ACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGc                                               >  1:4067675/1‑101 (MQ=255)
                                                   cccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGc                                               >  1:3732863/1‑101 (MQ=255)
                                                     cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTTGCGCCTTTTCGGCCCGCCGCGCAAGCGCGA.C.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcgc                                             >  2:2997441/1‑99 (MQ=255)
                                                       cAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCt                                           <  2:478962/101‑1 (MQ=255)
                                                            cGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCCCCTTTTGGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGc                                      <  2:4612137/101‑1 (MQ=255)
                                                            cGGAAGCCGCCGCCGGGCGGCGCGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCCCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCTGc                                      >  2:3812203/1‑101 (MQ=255)
                                                             ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                     >  2:1722748/1‑101 (MQ=255)
                                                              gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGccc                                    <  1:1921669/101‑1 (MQ=255)
                                                              gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCACGACGCCCCTCCGGccc                                    >  2:4189933/1‑101 (MQ=255)
                                                               aaGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCg                                   <  2:3061442/101‑1 (MQ=255)
                                                                 gccgccgccGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCa                                 >  1:3288686/1‑101 (MQ=255)
                                                                 gccgccgccGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCa                                 <  1:3242164/101‑1 (MQ=255)
                                                                   cgccgccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAgg                               <  2:4638806/101‑1 (MQ=255)
                                                                    gccgccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAggg                              >  2:2465777/1‑101 (MQ=255)
                                                                        ccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.AGC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGa                          >  2:4102178/1‑101 (MQ=255)
                                                                          gggCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGc                        <  2:4718847/101‑1 (MQ=255)
                                                                            gcggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                      >  2:2085588/1‑101 (MQ=255)
                                                                             cggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCg                     >  1:2353979/1‑101 (MQ=255)
                                                                             cggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCg                     >  2:1315822/1‑101 (MQ=255)
                                                                              ggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                    <  1:2972124/101‑1 (MQ=255)
                                                                              ggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                    >  1:1545559/1‑101 (MQ=255)
                                                                                cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  1:2600149/1‑101 (MQ=255)
                                                                                cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  2:4116311/1‑101 (MQ=255)
                                                                                cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  1:3052154/1‑101 (MQ=255)
                                                                                cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                  >  1:3280114/1‑101 (MQ=255)
                                                                                 gTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                 >  2:2851597/1‑101 (MQ=255)
                                                                                 gTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                 >  1:2335216/1‑101 (MQ=255)
                                                                                 gTGCTGTCGCGCCTTTGCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAACCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                 <  1:3385355/101‑1 (MQ=255)
                                                                                   gCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc               <  2:3075532/101‑1 (MQ=255)
                                                                                   gCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc               >  1:2168689/1‑101 (MQ=255)
                                                                                    cTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcg              <  1:4426567/101‑1 (MQ=255)
                                                                                     tGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc             >  1:320155/1‑101 (MQ=255)
                                                                                     tGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc             >  2:106063/1‑101 (MQ=255)
                                                                                      gTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg            >  1:1801472/1‑101 (MQ=255)
                                                                                      gTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg            >  1:2449972/1‑101 (MQ=255)
                                                                                        cgcgCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCNGCNNGCAGGGCCGAGCCCGGCCTGCGCggg          >  1:2639755/1‑101 (MQ=255)
                                                                                        cgcgCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg          >  1:3220060/1‑101 (MQ=255)
                                                                                        cgcgCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg          >  2:389200/1‑101 (MQ=255)
                                                                                         gcgcCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCgggc         >  2:3396776/1‑100 (MQ=255)
                                                                                         gcgcCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggt         >  1:867261/1‑101 (MQ=255)
                                                                                         gcgcCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggt         >  2:2486763/1‑101 (MQ=255)
                                                                                            ccTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcgg      >  2:1420391/1‑101 (MQ=255)
                                                                                              ttttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggtc    >  2:2916777/1‑101 (MQ=255)
                                                                                               tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   >  1:2775184/1‑101 (MQ=255)
                                                                                               tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   >  1:3039694/1‑101 (MQ=255)
                                                                                               tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   >  2:4337589/1‑101 (MQ=255)
                                                                                               tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   >  2:4506853/1‑101 (MQ=255)
                                                                                               tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   >  1:398772/1‑101 (MQ=255)
                                                                                               tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt   >  2:1495836/1‑101 (MQ=255)
                                                                                                ttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg  >  2:4134645/1‑101 (MQ=255)
                                                                                                ttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg  <  2:1545458/101‑1 (MQ=255)
                                                                                                ttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCCGGGCGGTCTg  >  1:4124915/1‑101 (MQ=255)
                                                                                                     |                                                                                                 
CCCCCGGTCGCCTCGGCCGCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGG.CCGCCGCGCAAGCGC.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTG  >  NC_014034/3173448‑3173642

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG