Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,173,565 +C 100% intergenic (+600/‑82) tyrA → / → RCAP_rcc02983 prephenate dehydrogenase/extensin family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,173,5651.→C100.0% 259.3 73intergenic (+600/‑82)tyrA/RCAP_rcc02983prephenate dehydrogenase/extensin family protein
Reads supporting (aligned to +/- strand):  new base (52/19):  ref base (2/0):  total (54/19)

GCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGG.CCGCCGCGCAAGCGC.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTG  >  NC_014034/3173466‑3173658
                                                                                                       |                                                                                             
gcgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.a                                                                                                <  2:2848550/101‑1 (MQ=255)
 cgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.ac                                                                                               <  1:1364602/101‑2 (MQ=255)
 cgcCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.ac                                                                                               <  2:959748/101‑2 (MQ=255)
    cGGTCCCGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgcc                                                                                            <  2:3864420/101‑5 (MQ=255)
     ggTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgccc                                                                                           >  1:3637495/1‑96 (MQ=255)
     ggTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgccc                                                                                           >  2:3452422/1‑96 (MQ=255)
     ggTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCgcgcaagcgc.a.acgccc                                                                                           >  2:3834021/1‑96 (MQ=255)
       tCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGccccc                                                                                         <  2:3217316/101‑1 (MQ=255)
          cccccccccccgccCCCCCCACCCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCccccgcc                                                                                      <  2:431357/99‑1 (MQ=255)
           gcccgcccCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCccccgccc                                                                                     <  2:1114759/101‑1 (MQ=255)
           gcccgcccCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCCCGCAAGCGC.A.ACGCccccgccc                                                                                     >  2:3074118/1‑101 (MQ=255)
            cccccccccgccCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCccccgcccc                                                                                    <  2:2109106/101‑1 (MQ=255)
              cgcccccgccccCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCgg                                                                                  >  2:299664/1‑101 (MQ=255)
               ccccccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGa                                                                                 <  2:1933653/100‑1 (MQ=255)
                  cccgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgc                                                                              <  1:1750719/101‑1 (MQ=255)
                    cgcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgc                                                                            >  1:3061630/1‑101 (MQ=255)
                     gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCCAGCGC.A.ACGCCCCCGCCCCGGAAgcgcc                                                                           >  1:538309/1‑101 (MQ=255)
                     gcccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.ACC.GCCCCCGCCCCGGAAgcccc                                                                           >  2:2315211/1‑101 (MQ=255)
                      cccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgccc                                                                          >  1:4639062/1‑101 (MQ=255)
                       ccccACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgcccg                                                                         >  2:3742330/1‑101 (MQ=255)
                            ccacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAgcgcccgcagcc                                                                    >  1:610319/1‑97 (MQ=255)
                             cccccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                                   <  2:3231587/97‑1 (MQ=255)
                             cacTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGcgcccgcgcccg                                                                   <  1:3834256/101‑1 (MQ=255)
                              acTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGa                                                                  >  1:3115922/1‑101 (MQ=255)
                               cTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGac                                                                 >  1:1050064/1‑100 (MQ=255)
                                ccccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAg                                                                <  1:3074306/100‑1 (MQ=255)
                                 ccggCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGCAGCGCCCGCGCCCGAAGc                                                               >  1:2709548/4‑101 (MQ=255)
                                 cccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.ACC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGc                                                               >  1:4067675/1‑101 (MQ=255)
                                 cccGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGc                                                               >  1:3732863/1‑101 (MQ=255)
                                   cGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTTGCGCCTTTTCGGCCCGCCGCGCAAGCGCGA.C.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGcgc                                                             >  2:2997441/1‑99 (MQ=255)
                                     cAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCt                                                           <  2:478962/101‑1 (MQ=255)
                                          cGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCCCCTTTTGGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGc                                                      <  2:4612137/101‑1 (MQ=255)
                                          cGGAAGCCGCCGCCGGGCGGCGCGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCCCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCTGc                                                      >  2:3812203/1‑101 (MQ=255)
                                           ggAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGcc                                                     >  2:1722748/1‑101 (MQ=255)
                                            gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGccc                                                    <  1:1921669/101‑1 (MQ=255)
                                            gAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCACGACGCCCCTCCGGccc                                                    >  2:4189933/1‑101 (MQ=255)
                                             aaGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCg                                                   <  2:3061442/101‑1 (MQ=255)
                                               gccgccgccGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCa                                                 >  1:3288686/1‑101 (MQ=255)
                                               gccgccgccGGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCa                                                 <  1:3242164/101‑1 (MQ=255)
                                                 cgccgccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAgg                                               <  2:4638806/101‑1 (MQ=255)
                                                  gccgccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAggg                                              >  2:2465777/1‑101 (MQ=255)
                                                      ccgGGCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.AGC.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGa                                          >  2:4102178/1‑101 (MQ=255)
                                                        gggCGGCGTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGc                                        <  2:4718847/101‑1 (MQ=255)
                                                          gcggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGccc                                      >  2:2085588/1‑101 (MQ=255)
                                                           cggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCg                                     >  2:1315822/1‑101 (MQ=255)
                                                           cggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCg                                     >  1:2353979/1‑101 (MQ=255)
                                                            ggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                                    <  1:2972124/101‑1 (MQ=255)
                                                            ggcgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCgg                                    >  1:1545559/1‑101 (MQ=255)
                                                              cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                                  >  1:3052154/1‑101 (MQ=255)
                                                              cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                                  >  1:3280114/1‑101 (MQ=255)
                                                              cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                                  >  2:4116311/1‑101 (MQ=255)
                                                              cgTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGcc                                  >  1:2600149/1‑101 (MQ=255)
                                                               gTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 >  1:2335216/1‑101 (MQ=255)
                                                               gTGCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 >  2:2851597/1‑101 (MQ=255)
                                                               gTGCTGTCGCGCCTTTGCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAACCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCt                                 <  1:3385355/101‑1 (MQ=255)
                                                                 gCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc                               >  1:2168689/1‑101 (MQ=255)
                                                                 gCTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgc                               <  2:3075532/101‑1 (MQ=255)
                                                                  cTGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcg                              <  1:4426567/101‑1 (MQ=255)
                                                                   tGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc                             >  2:106063/1‑101 (MQ=255)
                                                                   tGTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTgcgc                             >  1:320155/1‑101 (MQ=255)
                                                                    gTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg                            >  1:2449972/1‑101 (MQ=255)
                                                                    gTCGCGCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGcgcg                            >  1:1801472/1‑101 (MQ=255)
                                                                      cgcgCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCNGCNNGCAGGGCCGAGCCCGGCCTGCGCggg                          >  1:2639755/1‑101 (MQ=255)
                                                                      cgcgCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg                          >  1:3220060/1‑101 (MQ=255)
                                                                      cgcgCCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCggg                          >  2:389200/1‑101 (MQ=255)
                                                                       gcgcCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCgggc                         >  2:3396776/1‑100 (MQ=255)
                                                                       gcgcCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggt                         >  2:2486763/1‑101 (MQ=255)
                                                                       gcgcCTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggt                         >  1:867261/1‑101 (MQ=255)
                                                                          ccTTTTCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcgg                      >  2:1420391/1‑101 (MQ=255)
                                                                            ttttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggtc                    >  2:2916777/1‑101 (MQ=255)
                                                                             tttgggcccgccgCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGggtcggtcc                   >  2:976936/8‑100 (MQ=255)
                                                                             tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   >  1:3039694/1‑101 (MQ=255)
                                                                             tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   >  2:1495836/1‑101 (MQ=255)
                                                                             tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   >  1:2775184/1‑101 (MQ=255)
                                                                             tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   >  2:4506853/1‑101 (MQ=255)
                                                                             tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   >  2:4337589/1‑101 (MQ=255)
                                                                             tttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCt                   >  1:398772/1‑101 (MQ=255)
                                                                              ttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg                  <  2:1545458/101‑1 (MQ=255)
                                                                              ttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTg                  >  2:4134645/1‑101 (MQ=255)
                                                                              ttCGGCCCGCCGCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCCGGGCGGTCTg                  >  1:4124915/1‑101 (MQ=255)
                                                                                cggcccgccgCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcg                >  2:1100439/5‑101 (MQ=255)
                                                                                  gcccgccgCGCAGGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTgcggc              >  1:2567252/3‑101 (MQ=255)
                                                                                  gcccgccgCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCACGGGTCGGTCTgcggc              >  2:713685/3‑101 (MQ=255)
                                                                                    ccgccgCGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGt            >  1:4655012/1‑101 (MQ=255)
                                                                                     cgccgcGCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTg           <  1:3452634/101‑1 (MQ=255)
                                                                                        cgcgCAAGCGC.A.ACGCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGccc        >  2:118847/1‑101 (MQ=255)
                                                                                             aagcgc.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt   >  2:2563497/1‑101 (MQ=255)
                                                                                             aagcgc.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt   >  2:4094165/1‑101 (MQ=255)
                                                                                             aagcgc.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCt   >  2:3067034/1‑101 (MQ=255)
                                                                                              agcgc.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTg  >  2:3056777/1‑101 (MQ=255)
                                                                                              agcgc.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTg  >  1:624808/1‑101 (MQ=255)
                                                                                              agcgc.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTg  >  1:2102869/1‑101 (MQ=255)
                                                                                              agcgc.a.acgCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTg  <  2:3637266/101‑1 (MQ=255)
                                                                                                       |                                                                                             
GCGCCGGTCACGCCCGCCCCCGCCCCCACCACTCCCGCAGCCCGGAAGCCGCCGCCGGGCGGCGTGCTGTCGCGCCTTTTCGG.CCGCCGCGCAAGCGC.A.A.GCCCCCGCCCCGGAAGCGCCCGCGCCCGAAGCCCCTCCGGCCCGCAGGGCCGAGCCCGGCCTGCGCGGGTCGGTCTGCGGCGTGCCCGGCCTG  >  NC_014034/3173466‑3173658

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG