Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,164 +G 100% coding (1307/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1641.→G100.0% 512.0 135coding (1307/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (70/65):  ref base (0/0):  total (70/65)

Only 100 of 155 total aligned reads displayed.

TCGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCAG.TGG.AA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGA  >  NC_014034/3256064‑3256251
                                                                                                     |                                                                                                     
tCGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCt                                                                                                        >  1:1920929/1‑101 (MQ=255)
 cGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.g                                                                                                      <  2:3472217/101‑1 (MQ=255)
 cGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCCCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.g                                                                                                      <  1:3143376/101‑1 (MQ=255)
  ggTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGAGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.gg                                                                                                     >  2:2470968/1‑100 (MQ=255)
    tGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggcc                                                                                                   <  1:3453228/101‑4 (MQ=255)
    tGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggcc                                                                                                   <  2:3084163/101‑4 (MQ=255)
    gggCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggcc                                                                                                   <  2:1919971/100‑4 (MQ=255)
     ggCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggccg                                                                                                  >  1:478641/1‑97 (MQ=255)
     ggCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggccg                                                                                                  >  2:2854797/1‑97 (MQ=255)
     ggCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.ggccg                                                                                                  <  2:3276563/101‑5 (MQ=255)
       cGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.g                                                                                                >  1:1950933/1‑101 (MQ=255)
       cGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.g                                                                                                <  1:1033700/101‑1 (MQ=255)
        gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.gg                                                                                               >  1:1358027/1‑101 (MQ=255)
        gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.gg                                                                                               <  1:609274/101‑1 (MQ=255)
        gggggTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.gg                                                                                               >  1:1162339/1‑101 (MQ=255)
            gTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.ggcgca                                                                                           <  2:4414517/101‑5 (MQ=255)
              cGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.ggcgcagc                                                                                         <  2:165633/101‑7 (MQ=255)
               gATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.ggcgcagct                                                                                        >  1:3522819/1‑94 (MQ=255)
                aTCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.ggcgcagctg                                                                                       >  2:2472798/1‑93 (MQ=255)
                  cTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.ggcgcagctgga                                                                                     >  1:3709538/1‑91 (MQ=255)
                     ggCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.cc                                                                                <  1:830817/101‑1 (MQ=255)
                      gcgcGGCGGTGCAGCGCGTCGGGGCCGAGGGGCTTTTGCTCAAGGGCGGAGGGCACCGCATGGCCGCGGGTCTGCCCCTGGCCGA.GGCGCAGCTGG.AA.ccc                                                                               <  1:1510952/101‑2 (MQ=255)
                      gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.ccc                                                                               <  2:18118/101‑2 (MQ=255)
                      gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.ccc                                                                               <  2:1431452/101‑2 (MQ=255)
                       cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.cccg                                                                              <  1:2666633/101‑3 (MQ=255)
                       cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.cccg                                                                              <  2:1275629/101‑3 (MQ=255)
                        gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.cccgc                                                                             >  2:4329087/1‑98 (MQ=255)
                         cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.cccgcg                                                                            <  1:168420/101‑5 (MQ=255)
                         cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AA.cccgcg                                                                            <  1:1916595/101‑5 (MQ=255)
                           gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGAt                                                                           >  1:574258/1‑101 (MQ=255)
                           gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGAt                                                                           <  2:444501/101‑1 (MQ=255)
                             ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATgg                                                                         <  1:2785781/101‑1 (MQ=255)
                               tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTAAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggca                                                                       >  1:4218486/1‑99 (MQ=255)
                                gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcac                                                                      <  1:3051317/101‑4 (MQ=255)
                                gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcac                                                                      >  2:4118663/1‑98 (MQ=255)
                                gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcac                                                                      <  1:1424455/101‑4 (MQ=255)
                                 cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacg                                                                     >  1:1754641/1‑97 (MQ=255)
                                 cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacg                                                                     <  2:3029208/101‑5 (MQ=255)
                                 cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacg                                                                     >  2:1431393/1‑97 (MQ=255)
                                  aGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacgg                                                                    <  2:1162253/101‑6 (MQ=255)
                                     gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGGAAA.CCGCGATggcacggctg                                                                 >  1:616879/1‑93 (MQ=255)
                                      cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATgtcacggctgg                                                                >  1:2354383/1‑91 (MQ=255)
                                      cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacggctgg                                                                >  2:2196904/1‑92 (MQ=255)
                                      cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacggctgg                                                                >  1:1375740/1‑92 (MQ=255)
                                       gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcccggctggc                                                               >  2:264171/1‑91 (MQ=255)
                                       gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacggctggc                                                               >  2:1603235/1‑91 (MQ=255)
                                       gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacggctggc                                                               <  2:1477436/101‑11 (MQ=255)
                                       gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacggctggc                                                               >  1:1970782/1‑91 (MQ=255)
                                       gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATggcacggctggc                                                               >  1:2595433/1‑91 (MQ=255)
                                            ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.g.t                                                       >  1:2621361/1‑101 (MQ=255)
                                               cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.g.ttgc                                                    >  2:921962/1‑98 (MQ=255)
                                                tccgatcTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.g.ttgcc                                                   >  2:770399/7‑97 (MQ=255)
                                                  ggggCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGCCCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcg                                                 <  1:4482136/101‑7 (MQ=255)
                                                   gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.g.ttgctcgc                                                >  2:2040788/1‑94 (MQ=255)
                                                     gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGccc                                               >  1:2414166/1‑101 (MQ=255)
                                                      cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCg                                              <  1:2242462/101‑1 (MQ=255)
                                                       ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                             >  2:2374272/1‑101 (MQ=255)
                                                       ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                             >  2:2359202/1‑101 (MQ=255)
                                                       ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCgg                                             <  1:2228894/101‑1 (MQ=255)
                                                           gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAgg                                         <  1:4463355/101‑1 (MQ=255)
                                                           gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCCCGCCCGGCGgg                                         >  2:4686789/1‑101 (MQ=255)
                                                             tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgc                                       >  1:350947/1‑101 (MQ=255)
                                                             tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAGGgc                                       <  1:979901/101‑1 (MQ=255)
                                                             tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGG.CACGGCTGGCCGA.GTTGCTCGCCCGGCAGGgc                                       <  2:1754536/101‑1 (MQ=255)
                                                              cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAggggg                                      >  1:2802289/1‑99 (MQ=255)
                                                               aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGgc.g.c                                   >  1:2440977/1‑101 (MQ=255)
                                                               aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGC.AACCCGCGATGGCAC.GGCTGGCCGAGG.TACTCGCCCGGCCGGgg.g.c                                   >  1:2166548/1‑101 (MQ=255)
                                                                tctgcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGc.g.c.g                                 <  1:2186548/98‑1 (MQ=255)
                                                                aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAggggggg                                    >  1:4192183/1‑97 (MQ=255)
                                                                  ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCCCCCCGCAGGGC.G.C.ggg                               >  2:2790294/1‑100 (MQ=255)
                                                                   gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGG.GCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCCGGGc.g.c.gcgg                              >  2:2186424/1‑98 (MQ=255)
                                                                    cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGGTCGCCCGGCAGGgc.ggcgggc                               >  1:4001771/1‑95 (MQ=255)
                                                                    cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGGAGGGC.G.C.ggggc                             >  1:378473/1‑98 (MQ=255)
                                                                     ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.C.ggggac                            >  2:2921778/1‑97 (MQ=255)
                                                                     ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.C.gggcac                            <  2:1499717/101‑5 (MQ=255)
                                                                       cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGATGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gg                          >  2:4341233/1‑101 (MQ=255)
                                                                          gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGG.G.GGCAc.ggggc                       >  1:4719768/1‑98 (MQ=255)
                                                                          gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.ggggg                       >  1:2039066/1‑101 (MQ=255)
                                                                           cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gggcgg                      <  1:1431487/101‑5 (MQ=255)
                                                                                cATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccgg                  <  2:3190852/101‑1 (MQ=255)
                                                                                 aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.G.CGGGCACGGGcgggccggc                 >  1:1063846/1‑101 (MQ=255)
                                                                                 aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggc                 <  2:1356459/101‑1 (MQ=255)
                                                                                  tGGCCGCGGGGCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCCC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                <  1:535005/101‑1 (MQ=255)
                                                                                  gggCCGCGGGTCTCGCCCTGGCCGA.GGCGCAGCAGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                <  1:4082222/100‑1 (MQ=255)
                                                                                   ggCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg               <  2:2595281/101‑1 (MQ=255)
                                                                                   ggCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg               <  2:478590/101‑1 (MQ=255)
                                                                                    gCCGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCC.GGGACGGGCGGCCCGGCCgg              >  2:4608852/1‑100 (MQ=255)
                                                                                     ccGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGAt             <  2:1375646/101‑1 (MQ=255)
                                                                                     ccGCGGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGAt             <  2:3855488/101‑1 (MQ=255)
                                                                                      cgcgGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTc            >  1:3798680/1‑101 (MQ=255)
                                                                                      cgcgGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTc            >  1:3459888/1‑101 (MQ=255)
                                                                                      cgcgGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATc            <  1:2658402/101‑1 (MQ=255)
                                                                                       gcgGGTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGGGGGCCGGGCGATCt           >  2:1408251/1‑101 (MQ=255)
                                                                                           gTCTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCgg       <  1:4687045/101‑1 (MQ=255)
                                                                                             cTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt     <  1:3157167/101‑1 (MQ=255)
                                                                                             cTGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt     <  1:2040909/101‑1 (MQ=255)
                                                                                              tGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg    <  2:3522607/101‑1 (MQ=255)
                                                                                              tGACCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg    <  2:350913/101‑1 (MQ=255)
                                                                                                aCCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa  <  1:1209142/101‑1 (MQ=255)
                                                                                                aCCCTGGCCGA.GGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa  <  2:1738096/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
TCGGTGGCGGGGGTCGATCTGGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCAG.TGG.AA.CCGCGATGG.AA.GGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGA  >  NC_014034/3256064‑3256251

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG