Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,175 +C 100% coding (1318/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1751.→C100.0% 493.4 130coding (1318/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (64/65):  ref base (1/0):  total (65/65)

Only 100 of 131 total aligned reads displayed.

GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGG.AA.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256084‑3256257
                                                                                                |                                                                                              
  gcgcGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AA.ccc                                                                                      <  2:18118/101‑2 (MQ=255)
   cgcgGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AA.cccg                                                                                     <  2:1275629/101‑3 (MQ=255)
    gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AA.cccgc                                                                                    >  2:4329087/1‑98 (MQ=255)
    gcggcgGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AA.cccgc                                                                                    >  2:2823262/1‑98 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AA.cccgcg                                                                                   <  1:168420/101‑5 (MQ=255)
     cggcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AA.cccgcg                                                                                   <  1:1916595/101‑5 (MQ=255)
       gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGAt                                                                                  <  2:444501/101‑1 (MQ=255)
       gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGAt                                                                                  >  1:574258/1‑101 (MQ=255)
       gcggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGAt                                                                                  <  2:3114095/101‑1 (MQ=255)
        cggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATg                                                                                 <  2:4034436/101‑1 (MQ=255)
         ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATgg                                                                                <  1:2785781/101‑1 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcac                                                                             >  1:3553310/1‑98 (MQ=255)
            gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcac                                                                             <  1:1424455/101‑4 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacg                                                                            >  1:1754641/1‑97 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacg                                                                            <  2:3029208/101‑5 (MQ=255)
             cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacg                                                                            >  2:1431393/1‑97 (MQ=255)
                 gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAAA.CCGCGATggcacggctg                                                                        >  1:616879/1‑93 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATgtcacggctgg                                                                       >  1:2354383/1‑91 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacggctgg                                                                       >  1:3789201/1‑92 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacggctgg                                                                       >  1:3508527/1‑92 (MQ=255)
                  cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacggctgg                                                                       >  2:2196904/1‑92 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcccggctggc                                                                      >  2:264171/1‑91 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacggctggc                                                                      <  2:1477436/101‑11 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacggctggc                                                                      >  2:1603235/1‑91 (MQ=255)
                   gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATggcacggctggc                                                                      >  1:1970782/1‑91 (MQ=255)
                        ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.t                                                             >  1:2621361/1‑101 (MQ=255)
                          ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGG.CACGGCTGGCCG.A.g.ttg                                                           <  1:1148314/101‑3 (MQ=255)
                            tccgatcTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgcc                                                         >  2:770399/7‑97 (MQ=255)
                             aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctc                                                        >  2:2962196/1‑96 (MQ=255)
                              ggggCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGCCCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcg                                                       <  1:4482136/101‑7 (MQ=255)
                               gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgc                                                      >  2:2040788/1‑94 (MQ=255)
                                ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgcc                                                     >  1:2453342/1‑93 (MQ=255)
                                 gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccc                                                     >  1:2414166/1‑101 (MQ=255)
                                  cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCg                                                    <  1:2242462/101‑1 (MQ=255)
                                   ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                   >  2:2374272/1‑101 (MQ=255)
                                      tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCGCG.G.TGCTCGCCCGGCAg                                                >  2:2658248/1‑101 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                               >  2:1352101/1‑101 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                               <  1:4463355/101‑1 (MQ=255)
                                       gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCCCGCCCGGCGgg                                               >  2:4686789/1‑101 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                             >  1:350947/1‑101 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCGGCAgggg                                             >  1:1706393/1‑100 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                             <  1:979901/101‑1 (MQ=255)
                                         tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                             <  2:1754536/101‑1 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggg                                            >  1:2802289/1‑99 (MQ=255)
                                          cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc.g                                           <  2:1474308/101‑1 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc.g.c                                         >  1:2440977/1‑101 (MQ=255)
                                           aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGC.AACCCGCGATGGCAC.GGCTGGCCG.AGG.TACTCGCCCGGCCGGgg.g.c                                         >  1:2166548/1‑101 (MQ=255)
                                            tctgcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGc.g.c.g                                       <  1:2186548/98‑1 (MQ=255)
                                            aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggggg                                          >  1:4192183/1‑97 (MQ=255)
                                               gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGG.GCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCCGGGc.g.c.gcgg                                    >  2:2186424/1‑98 (MQ=255)
                                               gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgggggggggg                                       >  1:2732408/1‑94 (MQ=255)
                                               gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggc                                    <  1:2854973/101‑3 (MQ=255)
                                                cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGGTCGCCCGGCAGGgc.ggcgggc                                     >  1:4001771/1‑95 (MQ=255)
                                                cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGGAGGGC.G.C.ggggc                                   >  1:378473/1‑98 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCATC.TGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggccc                                  >  1:1496045/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.ggggac                                  >  2:2921778/1‑97 (MQ=255)
                                                 ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggcac                                  <  2:1499717/101‑5 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGc.g.c.gatcagc                                 <  2:2466140/101‑7 (MQ=255)
                                                  gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggcacg                                 <  1:751831/101‑6 (MQ=255)
                                                   cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGATGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gg                                >  2:4341233/1‑101 (MQ=255)
                                                      gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGG.G.GGCAc.ggggc                             >  1:4719768/1‑98 (MQ=255)
                                                      gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.ggggg                             >  1:2039066/1‑101 (MQ=255)
                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gggcgg                            <  1:1352193/101‑5 (MQ=255)
                                                       cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gggcgg                            <  1:1431487/101‑5 (MQ=255)
                                                        aCCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCAc.gggcggc                           >  2:1409502/1‑96 (MQ=255)
                                                          cGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggccc                          <  1:1942357/101‑1 (MQ=255)
                                                           gCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccg                         <  2:1920817/101‑1 (MQ=255)
                                                            cATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccgg                        <  2:3190852/101‑1 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCACGGGcgggccggc                       >  1:1063846/1‑101 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggc                       <  2:1356459/101‑1 (MQ=255)
                                                             aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCACGGC.G.CGGGCAc.gggccggccggc                      >  2:4177494/1‑91 (MQ=255)
                                                              tGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                      <  2:2327371/101‑1 (MQ=255)
                                                              tGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                      <  1:2242332/101‑1 (MQ=255)
                                                              tGGCCGCGGGGCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCCC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                      <  1:535005/101‑1 (MQ=255)
                                                              gggCCGCGGGTCTCGCCCTGGCCGA.GGCGCA.GCAGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                      <  1:4082222/100‑1 (MQ=255)
                                                               ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                     <  2:478590/101‑1 (MQ=255)
                                                               ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                     <  1:3054207/101‑1 (MQ=255)
                                                               ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                     <  2:2595281/101‑1 (MQ=255)
                                                                gCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.GCC.GGGACGGGCGGCCCGGCCgg                    >  2:4608852/1‑100 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGAt                   <  2:1375646/101‑1 (MQ=255)
                                                                 ccGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGAt                   <  2:3855488/101‑1 (MQ=255)
                                                                  cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTc                  >  1:3798680/1‑101 (MQ=255)
                                                                  cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATc                  <  1:2658402/101‑1 (MQ=255)
                                                                  cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATc                  <  1:264196/101‑1 (MQ=255)
                                                                   gcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGGGGGCCGGGCGATCt                 >  2:1408251/1‑101 (MQ=255)
                                                                     gggTCTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGc               >  2:1016674/1‑101 (MQ=255)
                                                                         cTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt           <  1:3157167/101‑1 (MQ=255)
                                                                         cTGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt           <  1:2040909/101‑1 (MQ=255)
                                                                          tGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg          <  2:350913/101‑1 (MQ=255)
                                                                          tGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg          <  2:3522607/101‑1 (MQ=255)
                                                                          tGACCCTGGCCGA.GGCGCA.GCTGG.AACCCGCCATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg          >  1:4227446/1‑101 (MQ=255)
                                                                            aCCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa        <  2:1738096/101‑1 (MQ=255)
                                                                            aCCCTGGCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa        <  1:1209142/101‑1 (MQ=255)
                                                                             ccctggCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc       <  2:1070414/96‑1 (MQ=255)
                                                                              cctggCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTCTGCGGCTGGGCg      >  2:232393/5‑101 (MQ=255)
                                                                               ctggCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg     >  1:486038/4‑101 (MQ=255)
                                                                               ctggCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg     <  1:4655592/98‑1 (MQ=255)
                                                                                tggCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg    <  2:2517356/99‑1 (MQ=255)
                                                                                 ggCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGc   >  2:625010/2‑101 (MQ=255)
                                                                                  gCCGA.GGCGCA.GCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt  <  1:3318283/101‑1 (MQ=255)
                                                                                                |                                                                                              
GGGCGCGGCGGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGG.AA.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCT  >  NC_014034/3256084‑3256257

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG