Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,189 +C 96.2% coding (1332/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1891.→C96.2% 5.5 ‑0.5 0.0105coding (1332/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (48/53):  ref base (1/3):  total (49/56)
Fisher's exact test strand distribution p-value = 6.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

GGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGG.AA.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGCG.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256093‑3256278
                                                                                                      |                                                                                                 
 gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggc                                                                                                   <  1:555873/101‑2 (MQ=255)
  tGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTAAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggca                                                                                                  >  1:4218486/1‑99 (MQ=255)
   gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcac                                                                                                 <  1:1424455/101‑4 (MQ=255)
   gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcac                                                                                                 <  1:3051317/101‑4 (MQ=255)
    cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacg                                                                                                >  2:1431393/1‑97 (MQ=255)
    cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacg                                                                                                >  1:2000278/1‑97 (MQ=255)
    cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacg                                                                                                >  1:1754641/1‑97 (MQ=255)
        gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAAA.CCGCGATggcacggctg                                                                                            >  1:616879/1‑93 (MQ=255)
         cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggctgg                                                                                           >  2:2196904/1‑92 (MQ=255)
         cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggctgg                                                                                           >  1:1375740/1‑92 (MQ=255)
         cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggctgg                                                                                           >  1:3508527/1‑92 (MQ=255)
         cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggctgg                                                                                           >  1:3789201/1‑92 (MQ=255)
          gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggctggc                                                                                          >  1:1970782/1‑91 (MQ=255)
          gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggctggc                                                                                          >  2:4707809/1‑91 (MQ=255)
          gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATggcacggctggc                                                                                          <  2:1477436/101‑11 (MQ=255)
               ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.t                                                                                 >  1:2621361/1‑101 (MQ=255)
                 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGG.CACGGCTGGCCG.A.g.ttg                                                                               <  1:1148314/101‑3 (MQ=255)
                   tccgatcTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgcc                                                                             >  2:770399/7‑97 (MQ=255)
                    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctc                                                                            >  2:2962196/1‑96 (MQ=255)
                    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctc                                                                            >  1:3973451/1‑96 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcg                                                                           <  1:4482136/101‑7 (MQ=255)
                      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgc                                                                          >  2:2040788/1‑94 (MQ=255)
                       ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgcc                                                                         >  1:2453342/1‑93 (MQ=255)
                        gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccc                                                                         >  1:2414166/1‑101 (MQ=255)
                         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCg                                                                        <  1:2242462/101‑1 (MQ=255)
                          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccccg                                                                       >  2:1787309/1‑99 (MQ=255)
                          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                       <  1:2228894/101‑1 (MQ=255)
                          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                       >  2:2374272/1‑101 (MQ=255)
                          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgc                                                                       >  2:2076447/1‑100 (MQ=255)
                             tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCGCG.G.TGCTCGCCCGGCAg                                                                    >  2:2658248/1‑101 (MQ=255)
                              gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                                                   >  2:1352101/1‑101 (MQ=255)
                              gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCCCGCCCGGCGgg                                                                   >  2:4686789/1‑101 (MQ=255)
                                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                 >  1:350947/1‑101 (MQ=255)
                                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCGGCAgggg                                                                 >  1:1706393/1‑100 (MQ=255)
                                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                 <  2:1754536/101‑1 (MQ=255)
                                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                 <  1:979901/101‑1 (MQ=255)
                                 cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggg                                                                >  1:2802289/1‑99 (MQ=255)
                                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgcg.c                                                              >  1:2440977/1‑101 (MQ=255)
                                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGC.AACCCGCGATGGCAC.GGCTGGCCG.AGG.TACTCGCCCGGCCGGggg.c                                                              >  1:2166548/1‑101 (MQ=255)
                                   tctgcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGcg.c.g                                                            <  1:2186548/98‑1 (MQ=255)
                                   aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggggg                                                              >  1:4192183/1‑97 (MQ=255)
                                     ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCCGCAGGGCG.C.ggg                                                          >  2:2790294/1‑100 (MQ=255)
                                      gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgggggggggg                                                           >  1:2732408/1‑94 (MQ=255)
                                      gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.C.gggc                                                         <  1:2854973/101‑3 (MQ=255)
                                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGGTCGCCCGGCAGGgcggcgggc                                                          >  1:4001771/1‑95 (MQ=255)
                                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGGAGGGCG.C.ggggc                                                        >  1:378473/1‑98 (MQ=255)
                                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.C.ggggac                                                       >  2:2921778/1‑97 (MQ=255)
                                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.C.gggcac                                                       <  2:1499717/101‑5 (MQ=255)
                                         gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGcg.c.gatcagc                                                      <  2:2466140/101‑7 (MQ=255)
                                          cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGATGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCAc.gg                                                     >  2:4341233/1‑101 (MQ=255)
                                             gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCAc.ggggg                                                  >  1:2039066/1‑101 (MQ=255)
                                              cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCAc.gggcgg                                                 <  1:1431487/101‑5 (MQ=255)
                                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGGG.CGGGCAc.gggcggc                                                >  2:1409502/1‑96 (MQ=255)
                                                 cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGcggccc                                               <  1:1942357/101‑1 (MQ=255)
                                                   cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGcggcccgg                                             <  2:3190852/101‑1 (MQ=255)
                                                    aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGGG.CGGGCACGGGcgggccggc                                            >  1:1063846/1‑101 (MQ=255)
                                                    aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGcggcccggc                                            <  2:1356459/101‑1 (MQ=255)
                                                    aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCACGGCG.CGGGCAc.gggccggccggc                                           >  2:4177494/1‑91 (MQ=255)
                                                     tGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGcggcccggcc                                           <  2:2327371/101‑1 (MQ=255)
                                                     tGGCCGCGGGGCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCCC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGcggcccggcc                                           <  1:535005/101‑1 (MQ=255)
                                                      ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCg                                          <  2:2595281/101‑1 (MQ=255)
                                                      ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCg                                          <  1:3054207/101‑1 (MQ=255)
                                                       gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCC.GGGACGGGCGGCCCGGCCgg                                         >  2:4608852/1‑100 (MQ=255)
                                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGAt                                        <  2:1375646/101‑1 (MQ=255)
                                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGAt                                        <  2:3855488/101‑1 (MQ=255)
                                                         cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGGTc                                       >  1:3459888/1‑101 (MQ=255)
                                                         cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATc                                       <  1:264196/101‑1 (MQ=255)
                                                         cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATc                                       <  1:2658402/101‑1 (MQ=255)
                                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGGGGGCCGGGCGATCt                                      >  2:1408251/1‑101 (MQ=255)
                                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCt                                      <  2:3511752/101‑1 (MQ=255)
                                                           cgGGTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCCCGGGCGGCCCGGCCGATCTg                                     >  2:1513222/1‑101 (MQ=255)
                                                            gggTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGc                                    >  2:1016674/1‑101 (MQ=255)
                                                              gTCTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCgg                                  <  1:4687045/101‑1 (MQ=255)
                                                                cTGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                <  1:3157167/101‑1 (MQ=255)
                                                                 tGACCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                               <  2:350913/101‑1 (MQ=255)
                                                                 tGACCCTGGCCGAGGCGCAGCTGG.AACCCGCCATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                               >  1:4227446/1‑101 (MQ=255)
                                                                   aCCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                             <  1:1209142/101‑1 (MQ=255)
                                                                   aCCCTGGCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                             <  2:1738096/101‑1 (MQ=255)
                                                                    ccctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  2:1070414/96‑1 (MQ=255)
                                                                    ccctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  1:178779/96‑1 (MQ=255)
                                                                    ccctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  2:2972853/96‑1 (MQ=255)
                                                                     cctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGGTCTGCGGCTGGGCg                           >  2:232393/5‑101 (MQ=255)
                                                                      ctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                          >  1:486038/4‑101 (MQ=255)
                                                                      ctggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                          <  1:4655592/98‑1 (MQ=255)
                                                                       tggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGGG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCCggggggg                         >  1:3757425/3‑96 (MQ=255)
                                                                       tggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg                         <  2:2517356/99‑1 (MQ=255)
                                                                        ggCCGAGGCGCAGCTGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGc                        >  2:625010/2‑101 (MQ=255)
                                                                             aggcgcagctGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                   <  2:512892/92‑1 (MQ=255)
                                                                              ggcgcagctGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:2790459/93‑1 (MQ=255)
                                                                                cgcagctGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATg                >  2:2748577/7‑101 (MQ=255)
                                                                                   agctGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg             <  2:3782793/98‑1 (MQ=255)
                                                                                    gctGG.AACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg            >  1:3937234/3‑101 (MQ=255)
                                                                                     ctGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg           <  1:1149062/100‑1 (MQ=255)
                                                                                      tGG.AACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGgc          >  2:3740273/1‑101 (MQ=255)
                                                                                        ggaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGGCGGGCCGGGTCCCGATGGCggggg         >  1:173729/6‑99 (MQ=255)
                                                                                         gaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTTGGCGGGGCCCCGATGCCGGgcgc        >  1:2020287/5‑101 (MQ=255)
                                                                                         gaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGGCGGgggc        >  1:2379455/5‑101 (MQ=255)
                                                                                          gccccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  2:4191937/98‑1 (MQ=255)
                                                                                            cccGCGATGGCAC.GGCTGGCCG.A.GTTGCTGGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc     <  2:2166424/100‑1 (MQ=255)
                                                                                              cgcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCG.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGa   <  1:2359335/101‑1 (MQ=255)
                                                                                                      |                                                                                                 
GGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGG.AA.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGCG.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256093‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG