Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,195 +G 100% coding (1338/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1951.→G100.0% 392.7 104coding (1338/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (48/56):  ref base (0/0):  total (48/56)

Only 100 of 108 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGGA.A.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278
                                                                                                 |                                                                                          
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGG.CACGGCTGGCCG.A.g.ttg                                                                                <  1:1148314/101‑3 (MQ=255)
    tccgatcTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgcc                                                                              >  2:770399/7‑97 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctc                                                                             >  1:3973451/1‑96 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctc                                                                             >  2:2962196/1‑96 (MQ=255)
      ggggCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGCCCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcg                                                                            <  1:4482136/101‑7 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgc                                                                           >  2:2040788/1‑94 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgcc                                                                          >  1:2453342/1‑93 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccc                                                                          >  1:2414166/1‑101 (MQ=255)
          cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCg                                                                         <  1:2242462/101‑1 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccccg                                                                        >  2:1787309/1‑99 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                        >  2:2359202/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                        <  1:2228894/101‑1 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                        >  2:2374272/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgc                                                                        >  2:2076447/1‑100 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAGC.CCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGc                                                                       <  2:437332/101‑1 (MQ=255)
              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCGCG.G.TGCTCGCCCGGCAg                                                                     >  2:2658248/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                                                    >  2:1352101/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                                                    <  1:4463355/101‑1 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCCCGCCCGGCGgg                                                                    >  2:4686789/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                  >  1:350947/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCGGCAgggg                                                                  >  1:1706393/1‑100 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                  <  1:979901/101‑1 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                  <  2:1754536/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggg                                                                 >  1:2802289/1‑99 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc.g                                                                <  2:1474308/101‑1 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc.g.c                                                              >  1:2440977/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGCA.ACCCGCGATGGCAC.GGCTGGCCG.AGG.TACTCGCCCGGCCGGgg.g.c                                                              >  1:2166548/1‑101 (MQ=255)
                    tctgcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGc.g.c.g                                                            <  1:2186548/98‑1 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggggg                                                               >  1:4192183/1‑97 (MQ=255)
                      ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCCGCAGGGC.G.C.ggg                                                          >  2:2790294/1‑100 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGG.GCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCCGGGc.g.c.gcgg                                                         >  2:2186424/1‑98 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgggggggggg                                                            >  1:2732408/1‑94 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggc                                                         <  1:2854973/101‑3 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGGTCGCCCGGCAGGgc.ggcgggc                                                          >  1:4001771/1‑95 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCATC.TGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggccc                                                       >  1:1496045/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.ggggac                                                       >  2:2921778/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggcac                                                       <  2:1499717/101‑5 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.C.gggcacg                                                      <  1:751831/101‑6 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGATGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gg                                                     >  2:4341233/1‑101 (MQ=255)
                              gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGG.G.GGCAc.ggggc                                                  >  1:4719768/1‑98 (MQ=255)
                              gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.ggggg                                                  >  1:2039066/1‑101 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gggcgg                                                 <  1:1431487/101‑5 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCAc.gggcgg                                                 <  1:1352193/101‑5 (MQ=255)
                                aCCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCAc.gggcggc                                                >  2:1409502/1‑96 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggccc                                               <  1:1942357/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccg                                              <  2:1396245/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccg                                              <  2:1920817/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccgg                                             <  2:3190852/101‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCACGGGcgggccggc                                            >  1:1063846/1‑101 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggc                                            <  2:1356459/101‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCACGGC.G.CGGGCAc.gggccggccggc                                           >  2:4177494/1‑91 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                           <  2:2327371/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                           <  1:2242332/101‑1 (MQ=255)
                                      tGGCCGCGGGGCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCCC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                           <  1:535005/101‑1 (MQ=255)
                                      gggCCGCGGGTCTCGCCCTGGCCGA.GGCGCA.GCAGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGcggcccggcc                                           <  1:4082222/100‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                                          <  2:478590/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                                          <  1:3054207/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCg                                          <  2:2595281/101‑1 (MQ=255)
                                        gCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.GCC.GGGACGGGCGGCCCGGCCgg                                         >  2:4608852/1‑100 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGAt                                        <  2:3855488/101‑1 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGAt                                        <  2:1375646/101‑1 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTc                                       >  1:3459888/1‑101 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTc                                       >  1:3798680/1‑101 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATc                                       <  1:264196/101‑1 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATc                                       <  1:2658402/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGGGGGCCGGGCGATCt                                      >  2:1408251/1‑101 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCt                                      <  2:3511752/101‑1 (MQ=255)
                                             gggTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGc                                    >  2:1016674/1‑101 (MQ=255)
                                               gTCTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCgg                                  <  1:4687045/101‑1 (MQ=255)
                                                 cTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                <  1:2040909/101‑1 (MQ=255)
                                                 cTGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                <  1:3157167/101‑1 (MQ=255)
                                                  tGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                               <  2:350913/101‑1 (MQ=255)
                                                  tGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                               <  2:3522607/101‑1 (MQ=255)
                                                  tGACCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCCATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                               >  1:4227446/1‑101 (MQ=255)
                                                    aCCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                             <  2:1738096/101‑1 (MQ=255)
                                                    aCCCTGGCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                             <  1:1209142/101‑1 (MQ=255)
                                                     ccctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  2:1070414/96‑1 (MQ=255)
                                                     ccctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  1:178779/96‑1 (MQ=255)
                                                     ccctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                            <  2:2972853/96‑1 (MQ=255)
                                                      cctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGGTCTGCGGCTGGGCg                           >  2:232393/5‑101 (MQ=255)
                                                       ctggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                          <  1:4655592/98‑1 (MQ=255)
                                                        tggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGG.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCCggggggg                         >  1:3757425/3‑96 (MQ=255)
                                                        tggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg                         <  2:2517356/99‑1 (MQ=255)
                                                         ggCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGc                        >  2:625010/2‑101 (MQ=255)
                                                          gCCGA.GGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                       <  1:3318283/101‑1 (MQ=255)
                                                                aggcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                   <  2:512892/92‑1 (MQ=255)
                                                                 ggcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                  <  1:2790459/93‑1 (MQ=255)
                                                                  gcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                 <  2:2871998/94‑1 (MQ=255)
                                                                   cgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATg                >  2:2748577/7‑101 (MQ=255)
                                                                      agctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg             <  2:3782793/98‑1 (MQ=255)
                                                                       gctGGA.ACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCgg            >  1:3937234/3‑101 (MQ=255)
                                                                        ctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg           <  1:1149062/100‑1 (MQ=255)
                                                                           ggaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGGCGGGCCGGGTCCCGATGGCggggg         >  1:173729/6‑99 (MQ=255)
                                                                            gaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGGCGGgggc        >  1:2379455/5‑101 (MQ=255)
                                                                             gccccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc       <  2:4191937/98‑1 (MQ=255)
                                                                               cccGCGATGGCAC.GGCTGGCCG.A.GTTGCTGGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc     <  2:2166424/100‑1 (MQ=255)
                                                                                 cgcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCCCGa   >  1:1990691/1‑101 (MQ=255)
                                                                                 cgcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGa   <  1:2359335/101‑1 (MQ=255)
                                                                                  gcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:1787418/101‑1 (MQ=255)
                                                                                  gcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGC.G.CGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc  <  1:3020521/101‑1 (MQ=255)
                                                                                                 |                                                                                          
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGGA.A.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGC.G.C.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAC  >  NC_014034/3256108‑3256278

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG