Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,201 +T 100% coding (1344/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2011.→T100.0% 376.7 98coding (1344/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (37/61):  ref base (0/0):  total (37/61)

Only 100 of 120 total aligned reads displayed.

GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGA.A.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCG  >  NC_014034/3256108‑3256292
                                                                                                         |                                                                                             
ggCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.t                                                                                              >  1:2621361/1‑101 (MQ=255)
  ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGG.CACGGCTGGCCG.A.g.ttg                                                                                            <  1:1148314/101‑3 (MQ=255)
    tccgatcTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgcc                                                                                          >  2:770399/7‑97 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctc                                                                                         >  1:3973451/1‑96 (MQ=255)
     aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctc                                                                                         >  2:2962196/1‑96 (MQ=255)
      ggggCTTTTGCTCAAGGGCGGCGGGCCCCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcg                                                                                        <  1:4482136/101‑7 (MQ=255)
       gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgc                                                                                       >  2:2040788/1‑94 (MQ=255)
        ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.g.ttgctcgcc                                                                                      >  1:2453342/1‑93 (MQ=255)
         gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccc                                                                                      >  1:2414166/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGccccg                                                                                    >  2:1787309/1‑99 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                                    >  2:2359202/1‑101 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgg                                                                                    <  1:2228894/101‑1 (MQ=255)
           ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCgc                                                                                    >  2:2076447/1‑100 (MQ=255)
            tttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGAGC.CCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGc                                                                                   <  2:437332/101‑1 (MQ=255)
              tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCGCG.G.TGCTCGCCCGGCAg                                                                                 >  2:2658248/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                                                                <  1:4463355/101‑1 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgg                                                                                >  2:1352101/1‑101 (MQ=255)
               gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCCCGCCCGGCGgg                                                                                >  2:4686789/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                              >  1:350947/1‑101 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCCCCCGGCAgggg                                                                              >  1:1706393/1‑100 (MQ=255)
                 tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGG.CACGGCTGGCCG.A.GTTGCTCGCCCGGCAGGgc                                                                              <  1:979901/101‑1 (MQ=255)
                  cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgcg                                                                             <  2:1474308/101‑1 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGgcgc                                                                            >  1:2440977/1‑101 (MQ=255)
                   aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGCA.ACCCGCGATGGCAC.GGCTGGCCG.AGG.TACTCGCCCGGCCGGgggc                                                                            >  1:2166548/1‑101 (MQ=255)
                    tctgcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGcgc.g                                                                          <  1:2186548/98‑1 (MQ=255)
                    aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAggggggg                                                                           >  1:4192183/1‑97 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAgggggggggg                                                                        >  1:2732408/1‑94 (MQ=255)
                       gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGC.gggc                                                                       <  1:2854973/101‑3 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGGTCGCCCGGCAGGgcggcgggc                                                                       >  1:4001771/1‑95 (MQ=255)
                        cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGGAGGGCGC.ggggc                                                                      >  1:378473/1‑98 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCATC.TGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGC.gggccc                                                                     >  1:1496045/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGC.ggggac                                                                     >  2:2921778/1‑97 (MQ=255)
                         ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGC.gggcac                                                                     <  2:1499717/101‑5 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGcgc.gatcagc                                                                    <  2:2466140/101‑7 (MQ=255)
                          gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGC.gggcacg                                                                    <  1:751831/101‑6 (MQ=255)
                           cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGATGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCAc.gg                                                                   >  2:4341233/1‑101 (MQ=255)
                              gCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCAc.ggggg                                                                >  1:2039066/1‑101 (MQ=255)
                               cACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCAc.gggcgg                                                               <  1:1431487/101‑5 (MQ=255)
                                aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGGGCGGGCAc.gggcggc                                                              >  2:1409502/1‑96 (MQ=255)
                                  cGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggccc                                                             <  1:1942357/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccg                                                            <  2:1920817/101‑1 (MQ=255)
                                   gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccg                                                            <  2:1396245/101‑1 (MQ=255)
                                    cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccgg                                                           <  2:3190852/101‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGGGCGGGCACGGGcgggccggc                                                          >  1:1063846/1‑101 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggc                                                          <  2:1356459/101‑1 (MQ=255)
                                     aTGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCACGGCGCGGGCAc.gggccggccggc                                                         >  2:4177494/1‑91 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                                                         <  1:2242332/101‑1 (MQ=255)
                                      tGGCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                                                         <  2:2327371/101‑1 (MQ=255)
                                      tGGCCGCGGGGCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCCC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                                                         <  1:535005/101‑1 (MQ=255)
                                      gggCCGCGGGTCTCGCCCTGGCCGAGGCGCA.GCAGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGcggcccggcc                                                         <  1:4082222/100‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCg                                                        <  1:3054207/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCg                                                        <  2:2595281/101‑1 (MQ=255)
                                       ggCCGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCg                                                        <  2:478590/101‑1 (MQ=255)
                                         ccGCGGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGAt                                                      <  2:1375646/101‑1 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGGTc                                                     >  1:3798680/1‑101 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATc                                                     <  1:2658402/101‑1 (MQ=255)
                                          cgcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATc                                                     <  1:264196/101‑1 (MQ=255)
                                           gcgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCt                                                    <  2:3511752/101‑1 (MQ=255)
                                            cgGGTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCCCGGGCGGCCCGGCCGATCTg                                                   >  2:1513222/1‑101 (MQ=255)
                                             gggTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGc                                                  >  2:1016674/1‑101 (MQ=255)
                                               gTCTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCgg                                                <  1:4687045/101‑1 (MQ=255)
                                                 cTGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                              <  1:3157167/101‑1 (MQ=255)
                                                  tGACCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                             <  2:3522607/101‑1 (MQ=255)
                                                    aCCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                           <  1:1209142/101‑1 (MQ=255)
                                                    aCCCTGGCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                           <  2:1738096/101‑1 (MQ=255)
                                                     ccctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                          <  1:178779/96‑1 (MQ=255)
                                                     ccctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                          <  2:2972853/96‑1 (MQ=255)
                                                      cctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGGTCTGCGGCTGGGCg                                         >  2:232393/5‑101 (MQ=255)
                                                       ctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                                        >  1:486038/4‑101 (MQ=255)
                                                       ctggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                                        <  1:4655592/98‑1 (MQ=255)
                                                        tggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGGGCGGGCACGGGCGGCCCGGCCGATCTGCGGCCggggggg                                       >  1:3757425/3‑96 (MQ=255)
                                                        tggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg                                       <  2:2517356/99‑1 (MQ=255)
                                                         ggCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGc                                      >  2:625010/2‑101 (MQ=255)
                                                          gCCGAGGCGCA.GCTGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                     <  1:3318283/101‑1 (MQ=255)
                                                               aggcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                 <  2:512892/92‑1 (MQ=255)
                                                                ggcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                <  1:2790459/93‑1 (MQ=255)
                                                                 gcgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                               <  2:2871998/94‑1 (MQ=255)
                                                                  cgcagctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATg                              >  2:2748577/7‑101 (MQ=255)
                                                                     agctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg                           <  2:3782793/98‑1 (MQ=255)
                                                                       ctGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                         <  1:1149062/100‑1 (MQ=255)
                                                                        tGGA.ACCCGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGgc                        >  2:3740273/1‑101 (MQ=255)
                                                                          ggaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGGCGGGCCGGGTCCCGATGGCggggg                       >  1:173729/6‑99 (MQ=255)
                                                                           gaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTTGGCGGGGCCCCGATGCCGGgcgc                      >  1:2020287/5‑101 (MQ=255)
                                                                           gaacccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGGCGGgggc                      >  1:2379455/5‑101 (MQ=255)
                                                                            gccccGCGATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                     <  2:4191937/98‑1 (MQ=255)
                                                                              cccGCGATGGCAC.GGCTGGCCG.A.GTTGCTGGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc                   <  2:2166424/100‑1 (MQ=255)
                                                                                cgcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCCCGa                 >  1:1990691/1‑101 (MQ=255)
                                                                                cgcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGa                 <  1:2359335/101‑1 (MQ=255)
                                                                                 gcgATGGCAC.GGCTGGCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                <  1:3020521/101‑1 (MQ=255)
                                                                                        gcacggctggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgc          <  1:1016753/92‑1 (MQ=255)
                                                                                            ggctggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg      <  2:3973209/96‑1 (MQ=255)
                                                                                            ggctggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg      <  2:2220878/96‑1 (MQ=255)
                                                                                              ctggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt    <  2:1097841/98‑1 (MQ=255)
                                                                                               tggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc   >  1:1476026/3‑101 (MQ=255)
                                                                                               tggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCgcgccggtc   >  1:4032665/3‑96 (MQ=255)
                                                                                               tggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc   <  1:4711787/99‑1 (MQ=255)
                                                                                                ggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  <  2:3798445/100‑1 (MQ=255)
                                                                                                ggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  >  1:2183718/2‑101 (MQ=255)
                                                                                                ggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  >  1:3658966/2‑101 (MQ=255)
                                                                                                ggCCG.A.GTTGCTCGCCCGGCAGGGCGCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg  >  2:2199190/2‑101 (MQ=255)
                                                                                                         |                                                                                             
GGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCA.G.TGGA.A.CCGCGATGG.AA.GGCT.GCCG.A.G.TGTTCGCCCGGCAGGGCGC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCG  >  NC_014034/3256108‑3256292

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG