Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,220 +G 100% coding (1363/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,2201.→G100.0% 384.4 100coding (1363/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (36/64):  ref base (0/0):  total (36/64)

Only 100 of 126 total aligned reads displayed.

AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGGAA.CCGCGATGG.AAGGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.GC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314
                                                                                                         |                                                                                               
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGgc.gc                                                                                                  >  1:2440977/1‑101 (MQ=255)
aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGCAACCCGCGATGGCACGGCTGGCCGAGG.TACTCGCCCGGCCGGgg.gc                                                                                                  >  1:2166548/1‑101 (MQ=255)
   ggcggcGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCCCCCCGCAGGGC.GC.ggg                                                                                              >  2:2790294/1‑100 (MQ=255)
    gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGG.GCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCCGGGc.gc.gcgg                                                                                             >  2:2186424/1‑98 (MQ=255)
    gcggcgGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC.gggc                                                                                             <  1:2854973/101‑3 (MQ=255)
     cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGGAGGGC.GC.ggggc                                                                                            >  1:378473/1‑98 (MQ=255)
      ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCATC.TGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GC.gggccc                                                                                           >  1:1496045/1‑97 (MQ=255)
       gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGc.gc.gatcagc                                                                                          <  2:2466140/101‑7 (MQ=255)
        cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGATGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCAc.gg                                                                                         >  2:4341233/1‑101 (MQ=255)
           gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGCGGG.GGCAc.ggggc                                                                                      >  1:4719768/1‑98 (MQ=255)
           gCACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCAc.ggggg                                                                                      >  1:2039066/1‑101 (MQ=255)
            cACCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCAc.gggcgg                                                                                     <  1:1431487/101‑5 (MQ=255)
             aCCGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.GCGGGCAc.gggcggc                                                                                    >  2:1409502/1‑96 (MQ=255)
               cGCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGcggccc                                                                                   <  1:1942357/101‑1 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGcggcccg                                                                                  <  2:1396245/101‑1 (MQ=255)
                gCATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGcggcccg                                                                                  <  2:1920817/101‑1 (MQ=255)
                 cATGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGcggcccgg                                                                                 <  2:3190852/101‑1 (MQ=255)
                  aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.GCGGGCACGGGcgggccggc                                                                                >  1:1063846/1‑101 (MQ=255)
                  aTGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCACGGC.GCGGGCAc.gggccggccggc                                                                               >  2:4177494/1‑91 (MQ=255)
                   tGGCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGcggcccggcc                                                                               <  1:2242332/101‑1 (MQ=255)
                   tGGCCGCGGGGCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCCCGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGcggcccggcc                                                                               <  1:535005/101‑1 (MQ=255)
                   gggCCGCGGGTCTCGCCCTGGCCGA.GGCGCA.GCAGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGcggcccggcc                                                                               <  1:4082222/100‑1 (MQ=255)
                    ggCCGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCg                                                                              <  2:478590/101‑1 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGAt                                                                            <  2:3855488/101‑1 (MQ=255)
                      ccGCGGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGAt                                                                            <  2:1375646/101‑1 (MQ=255)
                       cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGGTc                                                                           >  1:3798680/1‑101 (MQ=255)
                       cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGGTc                                                                           >  1:3459888/1‑101 (MQ=255)
                       cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATc                                                                           <  1:2658402/101‑1 (MQ=255)
                       cgcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATc                                                                           <  1:264196/101‑1 (MQ=255)
                        gcgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGGGGGCCGGGCGATCt                                                                          >  2:1408251/1‑101 (MQ=255)
                         cgGGTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCCCGGGCGGCCCGGCCGATCTg                                                                         >  2:1513222/1‑101 (MQ=255)
                          gggTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGc                                                                        >  2:1016674/1‑101 (MQ=255)
                            gTCTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCgg                                                                      <  1:4687045/101‑1 (MQ=255)
                              cTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                                                    <  1:2040909/101‑1 (MQ=255)
                              cTGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCt                                                                    <  1:3157167/101‑1 (MQ=255)
                               tGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                                                   <  2:350913/101‑1 (MQ=255)
                               tGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                                                   <  2:3522607/101‑1 (MQ=255)
                               tGACCCTGGCCGA.GGCGCA.GCTGGAACCCGCCATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTg                                                                   >  1:4227446/1‑101 (MQ=255)
                                 aCCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                                                 <  1:1209142/101‑1 (MQ=255)
                                 aCCCTGGCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGa                                                                 <  2:1738096/101‑1 (MQ=255)
                                  ccctggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                <  2:2972853/96‑1 (MQ=255)
                                  ccctggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                <  2:1070414/96‑1 (MQ=255)
                                  ccctggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGAc                                                                <  1:178779/96‑1 (MQ=255)
                                   cctggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGGTCTGCGGCTGGGCg                                                               >  2:232393/5‑101 (MQ=255)
                                    ctggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCgg                                                              >  1:486038/4‑101 (MQ=255)
                                    ctggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACgg                                                              <  1:4655592/98‑1 (MQ=255)
                                     tggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGG.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCCggggggg                                                             >  1:3757425/3‑96 (MQ=255)
                                     tggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACggg                                                             <  2:2517356/99‑1 (MQ=255)
                                      ggCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGc                                                            >  2:625010/2‑101 (MQ=255)
                                       gCCGA.GGCGCA.GCTGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                                                           <  1:3318283/101‑1 (MQ=255)
                                             aggcgcagctGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                                                       <  2:512892/92‑1 (MQ=255)
                                              ggcgcagctGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                                                      <  1:2790459/93‑1 (MQ=255)
                                               gcgcagctGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                                                     <  2:2871998/94‑1 (MQ=255)
                                                cgcagctGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCCGATg                                                    >  2:2748577/7‑101 (MQ=255)
                                                   agctGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCg                                                 <  2:3782793/98‑1 (MQ=255)
                                                     ctGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg                                               <  1:1149062/100‑1 (MQ=255)
                                                      tGGAACCCGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGgc                                              >  2:3740273/1‑101 (MQ=255)
                                                        gaacccGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCCGGTGGCGGgggc                                            >  1:2379455/5‑101 (MQ=255)
                                                         gccccGCGATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc                                           <  2:4191937/98‑1 (MQ=255)
                                                             cgcgATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGGCGCCCCGa                                       >  1:1990691/1‑101 (MQ=255)
                                                             cgcgATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGa                                       <  1:2359335/101‑1 (MQ=255)
                                                              gcgATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                                      <  1:3020521/101‑1 (MQ=255)
                                                              gcgATGGCACGGCTGGCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGAc                                      <  1:1787418/101‑1 (MQ=255)
                                                                    gcacggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCcgc                                <  1:1016753/92‑1 (MQ=255)
                                                                       cggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCt                             <  1:2472941/95‑1 (MQ=255)
                                                                        ggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg                            <  2:3973209/96‑1 (MQ=255)
                                                                        ggctggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTg                            <  2:2220878/96‑1 (MQ=255)
                                                                          ctggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGAt                          <  2:1097841/98‑1 (MQ=255)
                                                                           tggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATc                         >  1:1476026/3‑101 (MQ=255)
                                                                           tggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCgcgccggtc                         >  1:4032665/3‑96 (MQ=255)
                                                                            ggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                        >  2:2199190/2‑101 (MQ=255)
                                                                            ggCCGA.GTTGCTCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCg                        >  1:2183718/2‑101 (MQ=255)
                                                                               ccgagttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAg                      >  1:3473285/10‑101 (MQ=255)
                                                                                cgagttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAgg                     <  1:3250502/93‑1 (MQ=255)
                                                                                 gagttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    <  2:4719512/94‑1 (MQ=255)
                                                                                 gagttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGa                    >  1:3974305/8‑101 (MQ=255)
                                                                                 gagttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCCAGGa                    >  2:63078/8‑101 (MQ=255)
                                                                                  ggttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAt                   <  2:4485103/95‑1 (MQ=255)
                                                                                    ttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 >  2:2408122/5‑101 (MQ=255)
                                                                                    ttgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtct                 >  1:3081168/5‑101 (MQ=255)
                                                                                     tgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                >  2:4643965/4‑101 (MQ=255)
                                                                                     tgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                >  2:783335/4‑101 (MQ=255)
                                                                                     tgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                <  2:2414031/98‑1 (MQ=255)
                                                                                     tgctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGAtctc                <  1:3219337/98‑1 (MQ=255)
                                                                                      gctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               >  1:1413014/3‑101 (MQ=255)
                                                                                      gctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               >  1:3049861/3‑101 (MQ=255)
                                                                                      gctCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCg               <  1:3501706/99‑1 (MQ=255)
                                                                                        tCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc             <  2:3247125/101‑1 (MQ=255)
                                                                                        tCGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAc             <  1:2336037/101‑1 (MQ=255)
                                                                                         cGCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGAcc            <  2:1063767/101‑1 (MQ=255)
                                                                                          gCCCGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcg           >  2:3952028/1‑101 (MQ=255)
                                                                                            ccGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg         >  2:3970087/1‑101 (MQ=255)
                                                                                            ccGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACcgcg         <  1:2197028/101‑1 (MQ=255)
                                                                                             cGGCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGGTCCTGATGCCCGGCGCCGCGACGCCCCCGCCGATCGAGGATCTCGACCgcgc        >  2:3619576/1‑101 (MQ=255)
                                                                                               gcggggC.GCGGGCACGGGGGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      <  1:4609105/98‑1 (MQ=255)
                                                                                               gCAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCg      >  1:2447523/1‑101 (MQ=255)
                                                                                                cAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCgg     >  1:3673147/1‑101 (MQ=255)
                                                                                                cAGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCgg     >  1:704596/1‑101 (MQ=255)
                                                                                                 aGGGC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGc    >  2:1895309/1‑101 (MQ=255)
                                                                                                   ggC.GCGGGCACGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGccc  <  1:2743009/101‑1 (MQ=255)
                                                                                                         |                                                                                               
AAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGA.GG.GCA.G.TGGAA.CCGCGATGG.AAGGCT.GCCGA.G.TGTTCGCCCGGCAGGGC.GC.GGCAC.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGACGCCCGCGCTGATCGAGGATCTCGACCGCGCCGGCCC  >  NC_014034/3256127‑3256314

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG