BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,258,963	+C	100%	coding (778/1971 nt)	efeU →	ferrous iron permease EfeU

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,258,963	1	.→C	100.0%	61.8	211	coding (778/1971 nt)	efeU	ferrous iron permease EfeU
Reads supporting (aligned to +/- strand): new base (118/72): ref base (0/21): total (118/93)
Fisher's exact test strand distribution p-value = 8.19e-09
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.12e-02

Only 100 of 236 total aligned reads displayed.


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258863‑3259058


 aaTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.c                                                                                                <  2:1581661/101‑1 (MQ=255)


 aaTGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.c                                                                                                >  1:151784/1‑101 (MQ=255)


   tGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccc                                                                                              >  2:4126198/1‑100 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGGGTTCCTGGCCGTG.A.ccct                                                                                             >  2:1863733/1‑99 (MQ=255)


    gCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccct                                                                                             >  2:2274368/1‑99 (MQ=255)


      cTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.ccctgc                                                                                           >  1:3857285/1‑97 (MQ=255)


        cGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCg                                                                                          >  2:3605177/1‑101 (MQ=255)


        cGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCg                                                                                          >  1:751160/1‑101 (MQ=255)


         ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGGGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                         >  2:41675/1‑101 (MQ=255)


         ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                         >  2:4440723/1‑101 (MQ=255)


         ggTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGc                                                                                         >  1:2428232/1‑101 (MQ=255)


            cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                      <  1:1583406/101‑1 (MQ=255)


            cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                      >  2:4361252/1‑101 (MQ=255)


            cgcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGctct                                                                                      >  1:2648604/1‑101 (MQ=255)


             gcgGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCtt                                                                                     <  2:2904666/101‑1 (MQ=255)


              cgGGCCCCGTGCTTTCGGCGCTCTTTGGCTATCCCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTc                                                                                    <  1:1154898/101‑1 (MQ=255)


                 gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTg                                                                                 <  2:3857043/101‑1 (MQ=255)


                 gCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTg                                                                                 <  1:3662213/101‑1 (MQ=255)


                   aCCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTgcg                                                                               >  2:4054271/1‑101 (MQ=255)


                       tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           >  1:2279095/1‑101 (MQ=255)


                       tGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGcccc                                                                           <  1:1248107/101‑1 (MQ=255)


                        gCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcg                                                                          >  1:3397504/1‑101 (MQ=255)


                          tttCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCcgcg                                                                        >  1:328139/1‑101 (MQ=255)


                           ttCGGCGCTCTTTGGCTATCATGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCgcgc                                                                       >  1:3496082/1‑101 (MQ=255)


                            tCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcc                                                                      >  2:2408327/1‑101 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  2:1306276/101‑1 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgccc                                                                     <  1:372435/101‑1 (MQ=255)


                             cGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCAcgccc                                                                     >  1:4124572/1‑101 (MQ=255)


                              ggCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccg                                                                    >  1:4196485/1‑101 (MQ=255)


                                cgcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccgcc                                                                  >  1:924638/1‑101 (MQ=255)


                                cgcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGcgcccgcc                                                                  >  1:3151728/1‑101 (MQ=255)


                                 gcTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCgcccgccc                                                                 >  1:2075486/1‑101 (MQ=255)


                                   tctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGccccc                                                               >  1:3068698/1‑101 (MQ=255)


                                   tctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGccccc                                                               >  2:2997359/1‑101 (MQ=255)


                                   tctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGccccc                                                               >  2:1041328/1‑101 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                              <  1:1298881/101‑1 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                              >  1:4270278/1‑101 (MQ=255)


                                    ctTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGcccccc                                                              >  1:2131021/1‑101 (MQ=255)


                                     tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             <  1:82442/101‑1 (MQ=255)


                                     tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             >  1:3686259/1‑101 (MQ=255)


                                     tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             >  1:303387/1‑101 (MQ=255)


                                     tttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCt                                                             >  1:958660/1‑101 (MQ=255)


                                      ttGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCtt                                                            <  2:1970239/101‑1 (MQ=255)


                                        ggCTATCACGACAGCCCGACCCAGGGCGGGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTcc                                                          >  2:270570/1‑101 (MQ=255)


                                           tATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCa                                                       >  2:2298158/1‑101 (MQ=255)


                                           tATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCCCa                                                       >  1:4422695/1‑101 (MQ=255)


                                               aCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   <  1:4237710/101‑1 (MQ=255)


                                               aCGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAg                                                   <  1:894517/101‑1 (MQ=255)


                                                  aCAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGcc                                                >  1:3235759/1‑101 (MQ=255)


                                                   cAGCCCGACCCGGGGCGAGATCACACTCTGAGCCGCGTTCCTGGCCGTG.ACCCTGCCGCCCTTCCTGCGCCCCGCGCGCGCCCCCCTTCCGCAAAAGGCCt                                               <  2:788988/101‑1 (MQ=255)


                                                   cAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCt                                               >  2:3289919/1‑101 (MQ=255)


                                                    aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              <  1:4440973/101‑1 (MQ=255)


                                                    aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              <  1:2527323/101‑1 (MQ=255)


                                                    aGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTg                                              >  2:3785860/1‑101 (MQ=255)


                                                      cccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGAt                                            <  1:4726630/101‑1 (MQ=255)


                                                       ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATc                                           >  1:1061437/1‑101 (MQ=255)


                                                       ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATc                                           >  2:4073795/1‑101 (MQ=255)


                                                       ccGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTCCCCCAAAAGCccggttc                                           >  2:64827/1‑96 (MQ=255)


                                                         gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         >  1:29348/1‑101 (MQ=255)


                                                         gACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCg                                         >  2:1616004/1‑101 (MQ=255)


                                                          aCCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGa                                        >  1:3355912/1‑101 (MQ=255)


                                                            ccAGGGCGGGGGCCCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                      <  1:1323411/101‑1 (MQ=255)


                                                            ccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                      >  1:405797/1‑101 (MQ=255)


                                                            ccAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                      >  1:2033987/1‑101 (MQ=255)


                                                            ccAGGGCGAGGTCGCCATCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATg                                      <  1:1754507/101‑1 (MQ=255)


                                                             cAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                     >  2:4385905/1‑101 (MQ=255)


                                                             cAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGa                                     <  2:2524058/101‑1 (MQ=255)


                                                               gggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcc                                   >  1:4285918/1‑101 (MQ=255)


                                                               gggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAcc                                   >  1:2958444/1‑101 (MQ=255)


                                                                ggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAccc                                  <  1:1973677/101‑1 (MQ=255)


                                                                ggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAccc                                  <  1:1027876/101‑1 (MQ=255)


                                                                ggCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGAccc                                  <  2:1743945/101‑1 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCTTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGTCCTGATCCGATGACCCCtt                               <  2:151768/101‑1 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:42613/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:2006996/1‑101 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               <  1:3579762/101‑1 (MQ=255)


                                                                   gAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCtt                               >  2:4062341/1‑101 (MQ=255)


                                                                    gggTCGCCCTCTGGCCCGCTTCCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTc                              <  2:2773411/100‑1 (MQ=255)


                                                                    aGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTc                              <  2:4094253/101‑1 (MQ=255)


                                                                     ggTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             >  2:468047/1‑101 (MQ=255)


                                                                     ggTCCCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTcc                             <  2:2433763/101‑1 (MQ=255)


                                                                      gTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCg                            <  1:4131894/101‑1 (MQ=255)


                                                                       tCGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGc                           >  1:3938505/1‑101 (MQ=255)


                                                                        cGCCCTCTGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGca                          >  1:4641924/1‑101 (MQ=255)


                                                                             tctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCCAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                     >  1:2340842/1‑101 (MQ=255)


                                                                             tctGGGCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAgc                     <  2:546845/101‑1 (MQ=255)


                                                                                gggCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGcc                  >  2:1769986/1‑101 (MQ=255)


                                                                                gggCCGCGTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGcc                  <  2:3686030/101‑1 (MQ=255)


                                                                                  gCCGCGTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGt                <  1:1616104/101‑1 (MQ=255)


                                                                                   ccccgTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTc               <  2:3397298/98‑1 (MQ=255)


                                                                                    cgctTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTcc              <  2:4491939/101‑1 (MQ=255)


                                                                                     cgtttCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCg             <  2:1224770/98‑1 (MQ=255)


                                                                                      cgTTCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCgg            <  1:3299108/101‑1 (MQ=255)


                                                                                       gTTCCTGGCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCggg           >  2:3129510/1‑101 (MQ=255)


                                                                                        ttCCTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGgc          <  1:792375/101‑1 (MQ=255)


                                                                                           cTGGCCGTGCC.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcc       <  1:568456/101‑1 (MQ=255)


                                                                                              gCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctcc    <  1:1863845/101‑1 (MQ=255)


                                                                                              gCCGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGcctcc    >  2:2968310/1‑101 (MQ=255)


                                                                                                cGTG.ACCCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCtt  >  1:2824493/1‑101 (MQ=255)


                                                                                                cGTG.ACCCTGCCGCTCTTCCTGAGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCtt  >  2:2174447/1‑101 (MQ=255)


CAATGCCTCGGTCGCGGGCACCGTGCTTTCGGCGCTCTTTGGCTATCACGACAGCCCGACCCAGGGCGAGGTCGCCCTCTGGGCCGCGTTCCTGGCCGTG.A.CCTGCCGCTCTTCCTGCGCCCCGCGCCCGCCCCCCTTCCGCAAAAGGCCTGATCCGATGACCCCTTCCGCACAAGCGCCGTCCGGGCGCCTCCTT  >  NC_014034/3258863‑3259058

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG