Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,328,528 +G 100% intergenic (‑49/+11) entB ← / ← argF isochorismatase/ornithine carbamoyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,328,5281.→G100.0% 508.7 153intergenic (‑49/+11)entB/argFisochorismatase/ornithine carbamoyltransferase
Reads supporting (aligned to +/- strand):  new base (121/22):  ref base (0/4):  total (121/32)

Only 100 of 185 total aligned reads displayed.

GCGCCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A..CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGCCC  >  NC_014034/3328428‑3328622
                                                                                                      |                                                                                                
gcgcCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCaa.a                                                                                                   >  1:3121221/1‑101 (MQ=255)
    cTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A..gccc                                                                                             <  2:3800966/101‑1 (MQ=255)
      gCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A..gccccc                                                                                           >  2:3022145/1‑100 (MQ=255)
            cGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCtt                                                                                     >  1:2604546/1‑101 (MQ=255)
            cGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCtt                                                                                     >  2:3105757/1‑101 (MQ=255)
              cTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCa                                                                                   >  1:4525682/1‑101 (MQ=255)
               tttGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCAc                                                                                  >  1:721147/1‑101 (MQ=255)
               tttGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCAc                                                                                  >  2:4725314/1‑101 (MQ=255)
                  gCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTg                                                                               >  2:4189970/1‑101 (MQ=255)
                   ctggTCGATGATAAGAAGCCCGGTCTTCCCCATCGCCGTGCTTTTCATCTTGCCATAAATATCCGGGGGGTCGGGGGGCAA.AG.CCCCCA.GCCTTCACTTGc                                                                              <  2:673315/98‑1 (MQ=255)
                    aTGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGcc                                                                             >  1:2748523/1‑101 (MQ=255)
                     tGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGccc                                                                            <  2:2887009/101‑1 (MQ=255)
                        cGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCaaa                                                                         >  1:764759/1‑101 (MQ=255)
                         gatgatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAg                                                                        >  2:4062725/1‑101 (MQ=255)
                         gatgatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCGGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAg                                                                        <  1:1380108/101‑1 (MQ=255)
                          atgatCAGACGCGCGGTCTCGCCCCTCCCCGTGCTTTTCATCTTGCAATAAATACCCGGGGGGTGGGGGGGCAA.AC.CCCCCA.GCCTTCACTTGCCCAAAGc                                                                       <  2:1842234/101‑1 (MQ=255)
                            gatCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGcac                                                                     >  1:845108/1‑99 (MQ=255)
                             atCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCa                                                                    >  2:4510983/1‑101 (MQ=255)
                             atCAGAAGCGCGGTCTTCCCCCCCCCCGTCCCTTCCATCTTGCAAAAAATTCCGGGGGGGTGGGGGGCCAA.AC.CCCCCA.GCCTTCACTTGCCCAAAGCCCa                                                                    <  1:3516890/101‑1 (MQ=255)
                                aGAGGCGGGGTCTTCCCCATCGCCGTCCTTTTCATCTTCCAAAAAATATCCGGGGGGTCGGGGGGAAA.AC.CCCCCA.GCCTTCACTTGCCCAAAGCCCAgcg                                                                 <  1:2803040/101‑1 (MQ=255)
                                   ggcggGGTCTCCCCCATCGCCGTGCCTTTCATCTTGCCAAAAATATCGGGGGGGTCGGGGGCCAACC..CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCAt                                                              <  2:4380052/100‑1 (MQ=255)
                                   aGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCAt                                                              >  1:4219/1‑101 (MQ=255)
                                    gcgcGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATg                                                             <  2:823677/101‑1 (MQ=255)
                                     cgcgGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGa                                                            >  2:1691467/1‑101 (MQ=255)
                                           cTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCt                                                      >  1:4020780/1‑101 (MQ=255)
                                           cTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCt                                                      >  1:3907795/1‑101 (MQ=255)
                                           cTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCt                                                      >  1:1012847/1‑101 (MQ=255)
                                             tccccACCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCGGGGGGCAA.AC.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCttt                                                    <  2:1580155/99‑1 (MQ=37)
                                             tGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCttt                                                    >  2:3899911/1‑101 (MQ=255)
                                              gCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCtttt                                                   >  2:626745/1‑101 (MQ=255)
                                              cccctcccccGTCCCTTTCTTTTTCCCATAAATTCCCGGGGGGTCGGGGGGAAACC..CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCtttt                                                   <  1:3133929/93‑1 (MQ=255)
                                                ccATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTgc                                                 >  2:3385688/1‑101 (MQ=255)
                                                 cATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTgcg                                                >  1:2837537/1‑101 (MQ=255)
                                                  acggCCGTCCTTTTCTTTTTCCCAAAAATTCCGGGGGGGTCGGGGGGCAA.AC.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTgcgc                                               <  1:1446777/98‑1 (MQ=255)
                                                   tCGCCGTCCCTTTCATCTTCCCAAAAATATCCGGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCa                                              <  2:2231738/101‑1 (MQ=255)
                                                   ccccgttccttttCATCTTGCCAAAAATCCCCGGGGGGTCTGGGGCAAA.AC.CCCCCG.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCa                                              <  1:2037340/91‑1 (MQ=255)
                                                     gccttccttttCATCTTGCAATAAATACCCTGGGGGCCGGGGGACAACG..CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATg                                            <  2:1441677/93‑1 (MQ=25)
                                                     gCCGTGCTTTTCTTTTTGCAAAAAATATCGGGGGGGTGGGGGGAAAACC..CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATg                                            <  2:1876483/101‑1 (MQ=255)
                                                     gCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATg                                            >  2:1599577/1‑101 (MQ=255)
                                                     cccGTGCTTTTCATCTGGCCAAAAATACCCTGGGGGTCGGGGGCCAACC..CCCCCG.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATg                                            <  1:3786824/100‑1 (MQ=255)
                                                      cggtcccTTTCATCTTCCCAAAAATTTCCGGGGGGTTGGGGGGCAACG..CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGc                                           <  2:3639943/96‑1 (MQ=255)
                                                      ccGTGCCTTTCCTCTTGCCAAAAATATCCTGGGGGTTGGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGc                                           <  2:1967794/101‑1 (MQ=255)
                                                       cGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCTTTTTGCGCATGCa                                          >  1:3175984/1‑101 (MQ=255)
                                                        gTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCATCGCATGATCGCCTTTTGCGCATGCAg                                         >  1:3988993/1‑101 (MQ=255)
                                                        gTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAg                                         <  2:2033222/101‑1 (MQ=255)
                                                        gTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAg                                         >  1:117180/1‑101 (MQ=255)
                                                          gCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGcc                                       >  1:1601723/1‑101 (MQ=255)
                                                           ccTTTCATCTTGCCATAAATATCCGGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCg                                      <  2:570069/101‑1 (MQ=255)
                                                            cTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCgg                                     >  1:1523685/1‑101 (MQ=255)
                                                              ttCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGtt                                   >  1:815967/1‑101 (MQ=255)
                                                              ttCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGtt                                   >  1:3835674/1‑101 (MQ=255)
                                                               tttttctGCCAAAAAATTCGTGGGGGTGGGGGGGCAA.ACCCCCCCG.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGtt                                   <  1:4645572/95‑1 (MQ=255)
                                                               tCATCTTGCCTTAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGTCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTtc                                  >  2:3160274/1‑101 (MQ=38)
                                                               tCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTtc                                  >  2:4121321/1‑101 (MQ=255)
                                                               tCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTtc                                  >  1:4670854/1‑101 (MQ=255)
                                                                cATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTtct                                 >  2:4674031/1‑101 (MQ=255)
                                                                   cTTACCAAAAATATCCGGGGGGTCTGGGGGCAA.AC.CCCCCAGGCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTcc                               <  2:4026819/101‑1 (MQ=255)
                                                                     tccaaTAAATATCCTGGGGGTCTGGGGGAAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGcc                            <  2:318356/97‑1 (MQ=255)
                                                                     tGCCATAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGcc                            >  2:2532970/1‑101 (MQ=255)
                                                                         aTAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgt                        >  1:4349604/1‑101 (MQ=255)
                                                                         aTAAATATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgt                        >  1:1349880/1‑101 (MQ=255)
                                                                         aTAAATACCCGGGGGGTCGGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgt                        <  1:625506/101‑1 (MQ=255)
                                                                           aaaTTTCCGGGGGGGCGGGGGGCAA.AACCCCCCG.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtc                       <  1:1845341/101‑1 (MQ=255)
                                                                           aaaTATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtcg                      >  1:399283/1‑101 (MQ=255)
                                                                           aaaTATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtcg                      >  1:2882568/1‑101 (MQ=255)
                                                                           aaaTATCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtcg                      >  1:2918340/1‑101 (MQ=255)
                                                                           aaaTATCCGGGGGGTCTGGGGGCAA.AC.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcgtcg                      <  1:1588740/101‑1 (MQ=255)
                                                                              tatCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAg                   >  2:3446722/1‑101 (MQ=255)
                                                                              tatCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAg                   >  2:274915/1‑101 (MQ=255)
                                                                              tatCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAg                   >  1:4425979/1‑101 (MQ=255)
                                                                              tatCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTCTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAg                   >  1:4107695/1‑101 (MQ=255)
                                                                               atCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCTCCTTTTGCGCATGCAGCCGGTTCTCCGCCtcagatcgga                  >  1:2860821/1‑93 (MQ=255)
                                                                               atCCTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGa                  >  2:2620048/1‑101 (MQ=255)
                                                                               atCCTGGGGGACTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAGGa                  >  2:1038213/1‑101 (MQ=255)
                                                                                tCCTGGGGTTCCGGGGGGAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGAt                 <  2:2145279/101‑1 (MQ=255)
                                                                                  cTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCa               >  1:2517290/1‑101 (MQ=255)
                                                                                  cTGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCa               >  2:772278/1‑101 (MQ=255)
                                                                                   tGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCAc              >  2:3263749/1‑101 (MQ=255)
                                                                                   tGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCAc              >  2:3597952/1‑101 (MQ=255)
                                                                                   tGGGGGTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCAc              >  2:3993945/1‑101 (MQ=255)
                                                                                    gggggTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCAcc             <  1:2105093/101‑1 (MQ=255)
                                                                                    gggggGCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCAcc             >  2:3469316/1‑101 (MQ=255)
                                                                                     ggggTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCg            >  2:4594513/1‑101 (MQ=255)
                                                                                     ggggTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCg            <  2:1437975/101‑1 (MQ=255)
                                                                                      gggTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGa           >  1:1058776/1‑101 (MQ=255)
                                                                                        gTCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAAt         >  1:3433458/1‑101 (MQ=255)
                                                                                        gTCTGGGGCCAA.AC.CCCCCG.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAAt         <  1:2953963/101‑1 (MQ=255)
                                                                                        gTCGGGGGGCAA.AC.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCTCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAAt         <  1:3660081/101‑1 (MQ=255)
                                                                                         tCTGGGGGCAA.AG.CCCCCT.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATg        <  2:3175784/101‑1 (MQ=255)
                                                                                         tCTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATg        <  2:1509335/101‑1 (MQ=255)
                                                                                          cTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGc       >  1:968718/1‑101 (MQ=255)
                                                                                          cTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGc       >  2:1163240/1‑101 (MQ=255)
                                                                                          cTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGc       >  1:4580684/1‑101 (MQ=255)
                                                                                          cTGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGc       >  1:3635141/1‑101 (MQ=255)
                                                                                           tGGGGGCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCg      >  1:1511656/1‑101 (MQ=255)
                                                                                            gtgggCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCgg     >  2:353091/3‑101 (MQ=255)
                                                                                            gggggCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATCCgg     >  2:664343/1‑101 (MQ=255)
                                                                                             ggggCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGc    >  2:2824958/1‑101 (MQ=255)
                                                                                               ggCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGccc  >  1:3927214/1‑101 (MQ=255)
                                                                                               ggCAA.AG.CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGccc  >  2:2702900/1‑101 (MQ=255)
                                                                                                      |                                                                                                
GCGCCTGCATCTCGCTTTGCATGTCGATGATCAGAAGCGCGGTCTTGCCCATCGCCGTGCCTTTCATCTTGCCATAAATATCCTGGGGGTCTGGGGGCAA.A..CCCCCA.GCCTTCACTTGCCCAAAGCCCAGCGCATGATCGCCTTTTGCGCATGCAGCCGGTTCTCCGCCTCGTCGAAGATCACCGAATGCGGCCC  >  NC_014034/3328428‑3328622

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 29 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG