Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,394,236 +C 2.3% coding (787/831 nt) RCAP_rcc03194 ← hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,394,2361.→C 2.3% 4.7 ‑2.1 ‑0.0176coding (787/831 nt)RCAP_rcc03194hypothetical protein
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (86/86):  total (86/90)
Fisher's exact test strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.83e-01

Only 100 of 192 total aligned reads displayed.

CGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCCG.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCCGACCCAGCGTGCCAAGCGCCTTGCCCAGTTCCA  >  NC_014034/3394141‑3394331
                                                                                                 |                                                                                               
cgggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCtt                                                                                            <  1:1021887/101‑1 (MQ=255)
cgggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCtt                                                                                            >  1:47992/1‑101 (MQ=255)
 gggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTc                                                                                           <  1:732182/101‑1 (MQ=255)
 gggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTc                                                                                           <  1:142144/101‑1 (MQ=255)
  ggcgggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTcc                                                                                          >  2:513906/1‑101 (MQ=255)
  ggcgggACCCCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTcc                                                                                          <  1:3377354/101‑1 (MQ=255)
   gcgggCCCCCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCg                                                                                         <  1:3312042/101‑1 (MQ=255)
     gggACCTCCCCGCGCAGCAAGGCCGGGGGCAGAGCGCCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCgcg                                                                                       >  2:3174339/1‑99 (MQ=255)
     gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTTCCCGC.CGCTTCCGAg                                                                                       >  1:1493745/1‑101 (MQ=255)
     gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                       >  2:1504171/1‑101 (MQ=255)
     gggACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAg                                                                                       <  1:2019305/101‑1 (MQ=255)
      ggACCGCCCCGCTCAGCAAGGCCGCGCGCAGGGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGc                                                                                      <  2:658136/101‑1 (MQ=255)
      ggACCCCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGc                                                                                      <  1:112982/101‑1 (MQ=255)
         ccTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCgg                                                                                   >  2:2544670/1‑101 (MQ=255)
          cTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCggg                                                                                  >  2:3785761/1‑101 (MQ=255)
            ccccGCGCAGCAGGGCCGCGGGGGGAGGGT.AGGGTCCGGTCACGGTCAGCCAAGCGCCGCGTGCGCGGCAGCCCCGTTGCCCGC.CGCTTCCGAGCCGGGaa                                                                                <  2:3060688/101‑1 (MQ=255)
            ccccGCGCAGCAAGGCCGCGCGCGGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACCCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGaa                                                                                <  1:513957/101‑1 (MQ=255)
            ccccGCGCAGCAAGGCCGCGCGCAGAGTGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCTCCTTGCCCGC.CGCTTCCGACGCGGGaa                                                                                >  2:2350026/1‑101 (MQ=255)
             cccGCGCAGCAGGGCCGCGCGCGGGGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGaaa                                                                               <  1:2244680/101‑1 (MQ=255)
             cccGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGaaa                                                                               <  1:1319309/101‑1 (MQ=255)
              ccGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAAc                                                                              <  1:670482/101‑1 (MQ=255)
               cgcgCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAAcc                                                                             <  2:170701/101‑1 (MQ=255)
               cgcgCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAAcc                                                                             <  2:474513/101‑1 (MQ=255)
                gcgcAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACcg                                                                            <  2:799548/101‑1 (MQ=255)
                   cagcaAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCgcgc                                                                         >  1:1837669/1‑101 (MQ=255)
                    agcaAGGCCGCGCGCAGAGCGTCGGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGcc                                                                        <  1:2622068/101‑1 (MQ=255)
                     gcaAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCACCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCg                                                                       <  1:1091332/101‑1 (MQ=255)
                      cagggCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCgg                                                                      <  1:2431545/98‑1 (MQ=255)
                         ggCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGaaa                                                                   <  2:244672/101‑2 (MQ=255)
                          gCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGGCAAGGGACGCCTGGGCCGCAGCCGCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCCCGCCGGAAcc                                                                  >  2:3589783/1‑101 (MQ=255)
                           ccGCGCGCAGGGGGTCGGGCT.CGGTCCCGGCCAGCCAAGCGACGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCt                                                                 <  1:1123993/101‑1 (MQ=255)
                           ccGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCt                                                                 >  2:2841530/1‑101 (MQ=255)
                                cgcAGGGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTc                                                            <  1:4656233/101‑1 (MQ=255)
                                  cAGAGCGTCGGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAg                                                          <  2:2916117/101‑1 (MQ=255)
                                  cAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAg                                                          >  2:2206472/1‑101 (MQ=255)
                                   agagCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAgg                                                         >  2:330236/1‑101 (MQ=255)
                                     ggCGTCGGGGT.CGGTCAGGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGt                                                       <  1:4241621/100‑1 (MQ=255)
                                     agCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGt                                                       >  1:4191095/1‑101 (MQ=255)
                                      gCGTCGGGGT.CGGTCACGGGCAGCCAAGCGACGCGTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTc                                                      <  1:4077044/101‑1 (MQ=255)
                                      gCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTc                                                      <  2:1084799/101‑1 (MQ=255)
                                      gCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCCCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTc                                                      <  1:2981170/101‑1 (MQ=255)
                                       gctcggggT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcg                                                     <  2:1198558/96‑1 (MQ=255)
                                       cGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcg                                                     <  1:3775834/101‑1 (MQ=255)
                                        gTCGGGGT.CGGTCCCGGGCAGCCAAGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgc                                                    <  1:3713920/101‑1 (MQ=255)
                                        gTCGGGGT.CGGTCACGGGCAGCCAAGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgc                                                    <  1:2580466/101‑1 (MQ=255)
                                        gTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgc                                                    >  1:902889/1‑101 (MQ=255)
                                        gTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgc                                                    >  2:4046093/1‑101 (MQ=255)
                                        gTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgc                                                    >  1:4378633/1‑101 (MQ=255)
                                         tcggggT.GGGTCAGGGGCAGCCAAGCGACGCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgcg                                                   <  2:1837557/98‑1 (MQ=255)
                                         tcggggT.CGGTCCCGGGCACCCAAGCGACGTCTGCCCCGCAGCGCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgcg                                                   <  1:299371/98‑1 (MQ=255)
                                         tCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTcgcg                                                   >  2:2310945/1‑101 (MQ=255)
                                          cggggT.CGGTCCCGGGCAGCCAAGCGACGCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCgcgc                                                  <  1:4112924/99‑1 (MQ=255)
                                          cAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCgcgc                                                  >  1:4592929/1‑101 (MQ=255)
                                           aGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCa                                                 >  2:1748649/1‑101 (MQ=255)
                                            gggT.CGGCCCCGGGCGGCCAAGCGACGCCTGCCCCGCAGCCACCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAg                                                <  1:4731520/101‑1 (MQ=255)
                                              gt.cggtcACGGGCACCCAAGCGCCGCCTGCCCCGCAGCCCCCTGGCCCGC.CGCTCCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGc                                              <  1:3093236/101‑1 (MQ=255)
                                                 cggtcCCGGGCACCCAGGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCt                                            <  2:4439577/101‑1 (MQ=255)
                                                 cgctcAGGGGCAGCCAAGCGCCGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCt                                            <  2:4592676/98‑1 (MQ=255)
                                                  ggtcCCGGGCAGCCAAGCGCCGCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTc                                           <  1:2073367/101‑1 (MQ=255)
                                                  ggtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAAACTCGGTCAGGGTCGCGCAGGCCTc                                           >  2:1407568/1‑101 (MQ=255)
                                                   gtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                          >  1:2516470/1‑101 (MQ=255)
                                                   gtcACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCg                                          >  1:460845/1‑101 (MQ=255)
                                                     cACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGt                                        >  1:1642439/1‑101 (MQ=255)
                                                      cggggCAGCCAGGCGCCGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTc                                       <  2:4190849/99‑1 (MQ=255)
                                                      ccGGGCACCCAAGCGCCGCCTGCGCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTc                                       <  1:2175206/100‑1 (MQ=255)
                                                      aCGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCCGGCCTCGGTc                                       >  2:1109057/1‑101 (MQ=255)
                                                      aCGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTc                                       >  2:4498868/1‑101 (MQ=255)
                                                      aCGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTc                                       >  1:4299771/1‑101 (MQ=255)
                                                        gggCAGCCAAGCGCCCCCTGCGCCCCAGCCCCCTGGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCtt                                     <  1:1931634/101‑1 (MQ=255)
                                                        gggCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCtt                                     >  1:603641/1‑101 (MQ=255)
                                                        gggCAGCCAAGCGACGCCTGCGCCGCACCCACCTGGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCtt                                     <  2:2520146/101‑1 (MQ=255)
                                                          gCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTct                                   >  2:4644028/1‑100 (MQ=255)
                                                          gCAGCAAGGCGACCCGTGCGCGGCGGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTct                                   <  2:4604173/101‑2 (MQ=255)
                                                             gcaaggCGAGGCCGGGGCCGCGCCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAc                                <  1:1226919/96‑1 (MQ=255)
                                                             cccAAGCGACGCCTGCGCCGCAGCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAc                                <  1:2791984/100‑1 (MQ=255)
                                                               caggCGGCGCCGGCCCCGCACCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                              <  1:1983332/98‑1 (MQ=255)
                                                               cAAGCGCCGCCTGCCCCGCAGCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGAccc                              <  1:4133596/101‑1 (MQ=255)
                                                                aaGCGCCCCCTGCGCCCCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCa                             <  2:3041011/101‑1 (MQ=255)
                                                                aaGCGCCCCCTGCCCCGCAGCCCCCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCa                             <  1:3555883/101‑1 (MQ=255)
                                                                aaGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCa                             >  2:4125889/1‑101 (MQ=255)
                                                                aaGCGACGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCa                             >  1:3189155/1‑101 (MQ=255)
                                                                     aCGCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTg                        >  1:1202295/1‑101 (MQ=255)
                                                                       gCCTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGcc                      >  2:193842/1‑101 (MQ=255)
                                                                        cccgcgcCGCAGCCCCCTTGCCCGC.CGCTTCCGCGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCa                     <  1:4046335/98‑1 (MQ=255)
                                                                         cTGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCaa                    >  1:2519217/1‑101 (MQ=255)
                                                                          tGCGCCGCAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAg                   >  1:2722642/1‑101 (MQ=255)
                                                                            cgccgcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAgcg                 >  2:3847781/1‑101 (MQ=255)
                                                                            ccccgcACCCCCCTCCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAgcg                 <  1:2396553/99‑1 (MQ=255)
                                                                              ccgcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGcc               >  2:897345/1‑101 (MQ=255)
                                                                              ccgcAGCC.CCCTGCCCCGCCGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGcc               <  2:3485167/101‑1 (MQ=255)
                                                                               cgcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCt              >  1:4208693/1‑101 (MQ=255)
                                                                                gcggCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCtt             <  2:3346749/98‑1 (MQ=255)
                                                                                gcAGCCACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCtt             >  2:3550889/1‑101 (MQ=255)
                                                                                 caccccccTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTg            <  1:4030810/95‑1 (MQ=255)
                                                                                  aGCCCCCTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGc           <  2:3985169/101‑1 (MQ=255)
                                                                                   ccccccTTCCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGcc          <  2:2149544/97‑1 (MQ=255)
                                                                                    cccccTGGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc         <  1:3632964/98‑1 (MQ=255)
                                                                                    ccACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc         >  2:3811479/1‑101 (MQ=255)
                                                                                    ccACCTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGccc         >  1:955466/1‑101 (MQ=255)
                                                                                       ccTTGCCCGC.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCTGACCCAGCGTGCCAAGCGCCTTGCCCAGt      >  1:868646/1‑101 (MQ=255)
                                                                                                 |                                                                                               
CGGGCGGGACCTCCCCGCGCAGCAAGGCCGCGCGCAGAGCGTCAGGGT.CGGTCACGGGCAGCCAAGCGACGCCTGCGCCGCAGCCACCTTGCCCCG.CGCTTCCGAGCCGGGAAACCGCGCCGGAACCTCGGTCAGGGTCGCGCAGGCCTCGGTCTTCCGACCCAGCGTGCCAAGCGCCTTGCCCAGTTCCA  >  NC_014034/3394141‑3394331

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG