Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,439,831 +G 2.8% intergenic (‑134/+29) RCAP_rcc03240 ← / ← mutB ATPase AAA/methylmalonyl‑CoA mutase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,439,8312.→G 2.8% 4.9 ‑3.4 ‑0.0144intergenic (‑134/+29)RCAP_rcc03240/mutBATPase AAA/methylmalonyl‑CoA mutase large subunit
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (130/10):  total (132/12)
Fisher's exact test strand distribution p-value = 3.49e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

AAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCG  >  NC_014034/3439731‑3439933
                                                                                                       |                                                                                                       
  aGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGCAAGAT.AAAGGGGGTTTGCTTTTTTCTTTTCCCAAATA.TTCCCGGGGGGC..gg                                                                                                      >  2:2967951/1‑101 (MQ=37)
    aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..gggg                                                                                                    <  2:2839681/101‑1 (MQ=255)
    aCAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..gggg                                                                                                    <  1:3507544/101‑1 (MQ=255)
     cAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTTTTTTTTCCCAAATA.TCCCCGGGGGGC..ggggg                                                                                                   >  2:3540153/1‑101 (MQ=37)
      aGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGGCAGAT.AAAGGGGCTTTGCTTTTTTCTTTTCCCCAATA.TCCCCGGGGGGG..GGGGGc                                                                                                  >  2:2923855/1‑101 (MQ=37)
       ggAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCa                                                                                                 <  2:1267265/101‑1 (MQ=255)
         aaTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCaga                                                                                               <  2:4400773/101‑1 (MQ=255)
          aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAAAT.AAAGGGGGTTTGCTTTTTTTTTTCCCCCAATA.TCCCGGGGGGGG..GGGGGcgggg                                                                                              >  1:2902495/1‑97 (MQ=255)
          aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGGGATTTGGGGGAAGAT.AAAAGGGGTTTGGTTTTTTTTTTTCCCCAATATTCCCGGGGGGGG..gggggggag                                                                                               >  1:320001/1‑97 (MQ=255)
          aTTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCagag                                                                                              <  1:272806/101‑1 (MQ=255)
           ttCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGc                                                                                             <  2:2636525/101‑1 (MQ=255)
             cGGCCTGCGGCCGATGCCTCCGGCGGGGGTATTTTGGGGAAGATAAAAGGGGTTTGGTTTTTTTTTTTCCCCAAAAT.TCCCGGGGG.GC..gggggggggggcc                                                                                           >  2:4071818/1‑93 (MQ=255)
              ggCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGcccc                                                                                          <  1:3188538/101‑1 (MQ=255)
                gtgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTTATCTTTCCCCCAATA.TCCCGGGGGGCCCGGGGGGCAGCGccccc                                                                                         >  2:544815/9‑101 (MQ=21)
                 tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTGCCCCAAATA.TCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                                                        <  2:2970950/94‑2 (MQ=25)
                 tgactttcGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.GAA.ACCCATCCCTTTCATCTTGCCCCAAATA.TCCCGGGGGGCCCGGGGGGCAGCGcccccc                                                                                        <  1:4655605/94‑2 (MQ=25)
                 cTGCCGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCCcgc                                                                                       <  2:1548572/101‑1 (MQ=255)
                     ggCCGATGCCTCCGGCGGGGATATTTGGGGGAAGAT.AAAGGGGGTTTGCTTTTATCTTTTCCCCAATA.TCCCCGGGGTGC..GGGGGGCGAGccccccccgcc                                                                                   >  2:1221718/1‑95 (MQ=25)
                       ccGATGCCTCCGGCGGGGAGATTTTGGGGAAGAA.AAAGGGGCTTTGGTTTTATTTTTCCCCAAATA.TCCCGGGGGCGA..GGGGGGAGAAgccccaccgctcc                                                                                 >  2:3041378/1‑93 (MQ=19)
                           tGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCCcgccgccccggtt                                                                             >  2:2312820/1‑91 (MQ=255)
                             ccTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTTCCCAAATA.TACCGGGGGTGC..GGGGGCAGAGGCCCCGcccccaccggtca                                                                           >  2:4194336/1‑92 (MQ=18)
                                 cggaggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgcc                                                                     >  2:4140269/1‑101 (MQ=255)
                                   gcggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCCcgccgc                                                                   >  2:1286549/1‑101 (MQ=255)
                                   gcggGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCAcgccgc                                                                   >  1:1448653/1‑101 (MQ=255)
                                     ggggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGa                                                                 >  2:793766/1‑101 (MQ=255)
                                      gggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAc                                                                >  2:3320930/1‑101 (MQ=255)
                                       ggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                                                               >  2:4303726/1‑101 (MQ=255)
                                       ggATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGAcc                                                               >  1:3941347/1‑101 (MQ=255)
                                        gATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCg                                                              >  2:2529555/1‑101 (MQ=255)
                                           atTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                                           >  1:566597/1‑101 (MQ=255)
                                           atTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtct                                                           >  1:2535828/1‑101 (MQ=255)
                                            tttGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGtctc                                                          >  2:679157/1‑101 (MQ=255)
                                              tGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCg                                                        >  2:2428002/1‑101 (MQ=255)
                                               ggggCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                                                       >  1:1496583/1‑101 (MQ=255)
                                               ggggCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCgg                                                       >  1:2383916/1‑101 (MQ=255)
                                                gggCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCggg                                                      >  1:1963353/1‑101 (MQ=255)
                                                  gCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGt                                                    >  1:2058238/1‑101 (MQ=255)
                                                   cAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTc                                                   >  2:2796296/1‑101 (MQ=255)
                                                    aaGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                                  >  2:2150010/1‑101 (MQ=255)
                                                    aaGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                                  >  2:1361508/1‑101 (MQ=255)
                                                    aaGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCg                                                  >  2:1708896/1‑101 (MQ=255)
                                                       aT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                               >  1:611880/1‑101 (MQ=255)
                                                       aT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                               >  1:1833567/1‑101 (MQ=255)
                                                       aT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                               >  2:652420/1‑101 (MQ=255)
                                                       aT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCa                                               >  2:4647566/1‑101 (MQ=255)
                                                        t.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                              >  2:3608486/1‑101 (MQ=255)
                                                        t.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                              >  1:1592581/1‑101 (MQ=255)
                                                        t.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                              >  1:426436/1‑101 (MQ=255)
                                                        t.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                              >  2:2618055/1‑101 (MQ=255)
                                                        t.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                              >  1:3264867/1‑101 (MQ=255)
                                                        t.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAg                                              >  1:2950396/1‑101 (MQ=255)
                                                          aaaGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGCCGCCAgc                                             >  1:860870/1‑101 (MQ=255)
                                                           aaGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                            >  2:3770490/1‑101 (MQ=255)
                                                           aaGAGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcg                                            >  2:4022928/1‑101 (MQ=255)
                                                            aGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAgcgc                                           >  2:652870/1‑101 (MQ=255)
                                                              gggCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcga                                         >  1:86203/1‑101 (MQ=255)
                                                                 ctttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGcgacga                                      >  2:3368405/1‑101 (MQ=255)
                                                                  tttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat                                     >  2:3905663/1‑101 (MQ=255)
                                                                  tttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGat                                     >  1:4254429/1‑101 (MQ=255)
                                                                   ttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata                                    >  2:3901260/1‑101 (MQ=255)
                                                                   ttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata                                    >  1:3580896/1‑101 (MQ=255)
                                                                   ttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGata                                    >  1:2425732/1‑101 (MQ=255)
                                                                   ttgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAGCGCGACGata                                    >  1:508376/1‑101 (MQ=255)
                                                                    tgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat                                   >  2:1638016/1‑101 (MQ=255)
                                                                    tgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat                                   >  2:1453038/1‑101 (MQ=255)
                                                                    tgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatat                                   >  1:3251814/1‑101 (MQ=255)
                                                                    tgctttTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGatac                                   >  2:1387593/1‑100 (MQ=255)
                                                                       ttttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAGCGcgagatcggaag                                >  1:4482082/1‑92 (MQ=25)
                                                                         ttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                              >  2:2692151/1‑101 (MQ=255)
                                                                         ttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatc                              >  2:4677508/1‑101 (MQ=255)
                                                                         ttATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGGCGCCAGCGCGACGATATCcatc                              >  2:858285/1‑101 (MQ=255)
                                                                          tATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatca                             >  1:2892751/1‑101 (MQ=255)
                                                                          tATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCcatca                             >  1:915285/1‑101 (MQ=255)
                                                                            tCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATg                           >  2:2323651/1‑101 (MQ=255)
                                                                            tCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATg                           >  1:4403023/1‑101 (MQ=255)
                                                                             cTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGa                          >  1:2511871/1‑101 (MQ=255)
                                                                              tttCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGAt                         >  2:4456432/1‑101 (MQ=255)
                                                                              tttCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGAt                         >  2:1682982/1‑101 (MQ=255)
                                                                               ttCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATc                        >  1:1957487/1‑101 (MQ=255)
                                                                                tCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATcc                       >  2:369811/1‑101 (MQ=255)
                                                                                tCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATcc                       >  2:3240929/1‑101 (MQ=255)
                                                                                tCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATcc                       >  2:1999069/1‑101 (MQ=255)
                                                                                 ccccAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCg                      >  2:2825505/1‑101 (MQ=255)
                                                                                    cAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtt                   >  2:1556061/1‑101 (MQ=255)
                                                                                     aaaTA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGtta                  >  1:3968869/1‑100 (MQ=255)
                                                                                     aaaTA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTc                  >  1:110725/1‑101 (MQ=255)
                                                                                     aaaTA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGACATCCATCATGATCCGGTTc                  >  2:4739759/1‑101 (MQ=255)
                                                                                      aaTA.TCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCa                 >  1:1553613/1‑101 (MQ=255)
                                                                                       ata.tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAg                >  2:1768780/1‑101 (MQ=255)
                                                                                        ta.tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCTAGCGCGACGATATCCATCATGATCCGGTTCAGc               >  1:4086448/1‑101 (MQ=255)
                                                                                        ta.tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc               >  1:2650138/1‑101 (MQ=255)
                                                                                        ta.tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGc               >  1:591546/1‑101 (MQ=255)
                                                                                         a.tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCt              >  1:1254967/1‑101 (MQ=255)
                                                                                           tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc             >  2:2774972/1‑101 (MQ=255)
                                                                                           tCCCGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTc             >  1:1039126/1‑101 (MQ=255)
                                                                                            cccGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCg            >  1:3809203/1‑101 (MQ=255)
                                                                                              cGGGGGTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaa          >  1:329911/1‑101 (MQ=255)
                                                                                               gggggTGC..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGaaa         >  1:4163910/1‑101 (MQ=255)
                                                                                                      c..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCg  >  1:3014565/1‑101 (MQ=255)
                                                                                                      c..GGGGGCAGAGCCCC..CGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCg  >  1:798616/1‑101 (MQ=255)
                                                                                                       |                                                                                                       
AAAGACAGGAATTCGGCCTGCGGCCGATGCCTCCGGCGGGGATATTTGGGGCAAGAT.AAAGGGGCTTTGCTTTTATCTTTCCCCAAATA.TCCCGGGGGTGC..GGGGGCAGAGCCCCCGCGCCGCCACGGTTCACGCCGCGACCGTCTCCGGGGTCGCCAGCGCGACGATATCCATCATGATCCGGTTCAGCTCGAAATCCTTCG  >  NC_014034/3439731‑3439933

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG