BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	408,497	+A	85.5%	intergenic (+115/+33)	glnA1 → / ← dapA	glutamine synthetase/dihydrodipicolinate synthase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	408,497	5	.→A	85.5%	66.8 ‑0.4 ‑0.1	152	intergenic (+115/+33)	glnA1/dapA	glutamine synthetase/dihydrodipicolinate synthase
Reads supporting (aligned to +/- strand): new base (67/63): ref base (10/12): total (77/75)
Fisher's exact test strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.12e-01

Only 100 of 159 total aligned reads displayed.


CTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGGG.....GC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408396‑408571


ctGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACggggg                                                                                       >  1:2764908/1‑101 (MQ=255)


 gccccccAGCCCCCGGGAATTTTTGCGAAAAAAAGAACCCGATTTCCTTCATCTTCCCGAAAATACCCCGGGGGGAGGCTTGCTGAAAGC.AAGACggggg.....g                                                                                 <  1:60363/99‑1 (MQ=255)


  gCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gc                                                                                >  2:310404/1‑101 (MQ=255)


   cccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gca                                                                               <  1:3785162/101‑2 (MQ=255)


    ccccAGCCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcag                                                                              <  2:3368609/101‑3 (MQ=255)


    ccccAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcag                                                                              >  2:4176845/1‑99 (MQ=255)


        aGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTTATCTTGCCGCAAATATCCCGGGGGGACGCTTGCTGAAAGCAAA.ACGGGGG.....Gc....cgagcc                                                                      >  2:1335751/1‑96 (MQ=21)


         gACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagccc                                                                         <  1:447048/101‑8 (MQ=25)


          aCCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccc                                                                        >  1:2070641/1‑93 (MQ=25)


            cccgGGGATATGTGCGGCAGAATAAATCCGTTGTCCTTCAGCTTGCGGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                      <  2:2089677/101‑11 (MQ=16)


            ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                      <  1:4555551/101‑11 (MQ=25)


            ccccGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....Gcagagcccccc                                                                      <  2:2910302/101‑11 (MQ=25)


                        tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGcc                                                         >  1:107750/1‑101 (MQ=21)


                        tGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGcc                                                         <  1:4177091/101‑1 (MQ=21)


                         ggggcagaaTGAACCCGATGTCCTTCATTTGGCCGCAAATTCCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCa                                                        <  1:2631078/93‑1 (MQ=255)


                          gggCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCaa                                                       <  2:629448/100‑1 (MQ=21)


                          cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCaa                                                       <  1:4570327/101‑1 (MQ=21)


                          cGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCaa                                                       <  2:4351998/101‑1 (MQ=21)


                             cAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAg                                                    >  2:3693208/1‑101 (MQ=21)


                              acgctGAAGCCGAGGTCCTACCCTTCGCCGCACATCTCCCGGGGTGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAgg                                                   <  2:83278/97‑1 (MQ=255)


                              aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCCCAGGTCGCCAAAAgg                                                   >  2:406303/1‑101 (MQ=18)


                              aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGTCAAAAgg                                                   >  1:1334247/1‑101 (MQ=18)


                              aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAgg                                                   <  1:4398923/101‑1 (MQ=21)


                              aaGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAgg                                                   >  2:3681651/1‑101 (MQ=21)


                               aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGga                                                  <  1:564096/101‑1 (MQ=21)


                               aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGga                                                  <  2:3083671/101‑1 (MQ=21)


                               aGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGga                                                  >  1:1156810/1‑101 (MQ=21)


                                 aTGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGgacc                                                <  2:768647/101‑1 (MQ=21)


                                    aaGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGgaccgac                                             >  1:694521/1‑101 (MQ=21)


                                     aGCCGCTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGgaccgacc                                            <  1:4664193/101‑1 (MQ=18)


                                     aGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGgaccgacc                                            >  2:3464337/1‑101 (MQ=21)


                                      gcggATGTCCTTCATCTTGCGGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCt                                           <  1:1142724/98‑1 (MQ=18)


                                       ctgATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTc                                          <  2:381186/99‑1 (MQ=21)


                                       ccGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTc                                          <  2:1964719/101‑1 (MQ=21)


                                       ccGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTc                                          <  1:2992431/101‑1 (MQ=21)


                                       ccGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTc                                          <  2:2961640/101‑1 (MQ=21)


                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         >  1:555903/1‑101 (MQ=21)


                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         <  2:1083838/101‑1 (MQ=21)


                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         <  2:2082167/101‑1 (MQ=21)


                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         <  1:4645935/101‑1 (MQ=21)


                                        cGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCa                                         >  2:3610045/1‑101 (MQ=21)


                                         gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  1:367171/1‑101 (MQ=21)


                                         gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        <  2:4237759/101‑1 (MQ=21)


                                         gATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAg                                        >  2:1834243/1‑101 (MQ=21)


                                          aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       >  2:3513408/1‑101 (MQ=21)


                                          aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGa                                       <  2:4707322/101‑1 (MQ=21)


                                          aTGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....GCAGAGCAC.CCCGCAAAGGTCGCCAAAAGGACCGACCTCAGa                                       >  2:2235749/1‑101 (MQ=21)


                                           tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                      >  2:4349690/1‑101 (MQ=21)


                                           tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                      >  2:14597/1‑101 (MQ=21)


                                           tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                      <  2:4564739/101‑1 (MQ=21)


                                           tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                      <  2:1035150/101‑1 (MQ=21)


                                           tGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGAt                                      >  1:8071/1‑101 (MQ=21)


                                            gTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATc                                     <  1:1176039/101‑1 (MQ=21)


                                             tCCTTCTTCTTGCCGCAAATATCCGGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                    <  1:336950/101‑1 (MQ=21)


                                             tCCTTCCTCGTGGCGCAAATAACCCGGGGGGAGGCTTGCTGAAA.CCAGGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGCCCGACCTCAGATCa                                    <  2:237446/101‑1 (MQ=255)


                                             tCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCa                                    <  1:1191833/101‑1 (MQ=21)


                                              ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  2:4122128/1‑101 (MQ=21)


                                              ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  1:3504248/1‑101 (MQ=21)


                                              ccTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGG.....GCAGAGC.CGCCTGCACAGGTCGCCAAAAGGACCGACCTCAGATCAg                                   >  1:4055294/1‑101 (MQ=21)


                                               cTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGc                                  <  2:2790621/101‑1 (MQ=21)


                                                ttCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                 <  1:161612/101‑1 (MQ=21)


                                                ttCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGcc                                 >  1:4293556/1‑101 (MQ=21)


                                                 tCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGccc                                >  1:965940/1‑101 (MQ=21)


                                                  cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               >  2:4128491/1‑101 (MQ=21)


                                                  cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               >  1:1401202/1‑101 (MQ=21)


                                                  cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               >  2:2894076/1‑101 (MQ=21)


                                                  cATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCg                               >  1:4626354/1‑101 (MQ=21)


                                                   aTCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCgg                              <  2:1277280/101‑1 (MQ=21)


                                                    tCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGc                             >  2:1079770/1‑101 (MQ=21)


                                                     cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  2:677805/1‑101 (MQ=25)


                                                     cTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCg                            >  2:2197868/1‑101 (MQ=25)


                                                      ttGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgt                           >  2:1506314/1‑101 (MQ=25)


                                                       tGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtg                          >  1:3654631/1‑101 (MQ=25)


                                                        gCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCgtgt                         >  1:4708195/1‑101 (MQ=25)


                                                         ccGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGACCGGCGTGtt                        >  1:4722568/1‑101 (MQ=25)


                                                          cGCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTg                       <  1:1866876/101‑1 (MQ=25)


                                                           gCAAATATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGc                      >  1:1342721/1‑101 (MQ=25)


                                                             aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:640850/1‑101 (MQ=37)


                                                             aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:2805043/1‑101 (MQ=37)


                                                             aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    <  2:1440288/101‑1 (MQ=255)


                                                             aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  1:739941/1‑101 (MQ=37)


                                                             aaaTATCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCAt                    >  2:2591439/1‑101 (MQ=37)


                                                               atatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcg                  <  2:2069619/101‑1 (MQ=255)


                                                                tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgc                 >  2:750749/1‑101 (MQ=255)


                                                                tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgc                 >  1:1001358/1‑101 (MQ=255)


                                                                tatCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCGCAGATCAGCCCGGCGGGTTGCATcgc                 >  2:1392174/1‑101 (MQ=255)


                                                                 atCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                <  1:1929689/101‑1 (MQ=255)


                                                                 atCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                >  1:2135247/1‑101 (MQ=255)


                                                                 atCCCGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATcgcg                >  2:1693287/1‑101 (MQ=255)


                                                                    ccGGGGGGAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCgcggcg             >  2:354535/1‑101 (MQ=255)


                                                                      ggggggAGGCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           <  1:1705074/101‑1 (MQ=255)


                                                                      ggggggAGGCTTGCTGAAAGC.AAGACGGGGGGAAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           >  2:4616621/1‑101 (MQ=255)


                                                                      ggggggAGGCTTGCCGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTc           <  1:415309/101‑1 (MQ=255)


                                                                             ggCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGcgcg    >  1:3771830/1‑101 (MQ=255)


                                                                             ggCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGcgcg    <  2:2764744/101‑1 (MQ=255)


                                                                              gCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCgg   <  1:2645221/101‑1 (MQ=255)


                                                                              gCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCgg   <  1:1552878/101‑1 (MQ=255)


                                                                              gCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCgg   <  2:2611589/101‑1 (MQ=255)


                                                                              gCTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCACgg   >  1:3331365/1‑101 (MQ=255)


                                                                               cTTGCTGAAAGC.AAGACGGGGGGCAGAGC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGc  >  1:4692666/1‑101 (MQ=255)


CTGCCCCCAGACCCCCGGGATATTTGCGGCAAGATGAAGCCGATGTCCTTCATCTTGCCGCAAATATCCCGGGGGGAGGCTTGCTGAAAGCAAAGACGGGGG.....GC....C.C.CCCGCACAGGTCGCCAAAAGGACCGACCTCAGATCAGCCCGGCGTGTTGCATCGCGGCGTCAAGCGCGGC  >  NC_014034/408396‑408571

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 40 ≤


ATCG