Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 530,939 +G 2.3% intergenic (+17/+124) valS → / ← RCAP_rcc00497 valyl‑tRNA synthetase/UbiA prenyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034530,9392.→G 2.3% 2.3 0.0 ‑0.1131intergenic (+17/+124)valS/RCAP_rcc00497valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand):  new base (1/2):  ref base (48/80):  total (49/82)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.40e-01

Only 100 of 165 total aligned reads displayed.

GAAGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..CCGCGCTTGTCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530839‑531022
                                                                                                     |                                                                                    
gAAGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGGTc                                                                                       >  2:1317315/1‑101 (MQ=255)
gAAGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTc                                                                                       <  2:618870/101‑1 (MQ=255)
  aGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctg                                                                                     <  1:941021/101‑3 (MQ=255)
   gCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgc                                                                                    <  2:911775/101‑4 (MQ=255)
   gCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgc                                                                                    <  2:3098411/101‑4 (MQ=255)
    cccGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcc                                                                                   >  1:1126181/1‑97 (MQ=255)
    cccGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcc                                                                                   <  1:3682047/101‑5 (MQ=255)
       gcgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccc                                                                                <  2:2056986/101‑8 (MQ=255)
       gcgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccc                                                                                >  2:361263/1‑94 (MQ=255)
       gcgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccc                                                                                <  1:65438/101‑8 (MQ=255)
        cgAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgcccccc                                                                               <  2:679538/101‑9 (MQ=255)
         gAAATCCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCGGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccccg                                                                              <  2:3972827/101‑10 (MQ=255)
         gAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTctgccccccg                                                                              <  2:1710596/101‑10 (MQ=255)
          aaaaCCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTt..ccccccccgc                                                                           >  1:2346106/1‑93 (MQ=255)
                              gAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATAtt                                                        <  2:1579956/101‑1 (MQ=25)
                               aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATAttt                                                       >  2:4599091/1‑101 (MQ=25)
                                agagGCCGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATAtttt                                                      <  1:779504/101‑1 (MQ=21)
                                    gcggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGc                                                  <  1:3853696/101‑1 (MQ=25)
                                     cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:3140050/101‑1 (MQ=25)
                                      ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCaa                                                <  1:70726/101‑1 (MQ=25)
                                      ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCaa                                                <  2:100231/101‑1 (MQ=25)
                                       gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAg                                               <  2:1182422/101‑1 (MQ=25)
                                       gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAg                                               <  1:1317407/101‑1 (MQ=25)
                                        cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              >  2:3249919/1‑101 (MQ=21)
                                        cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  1:3739801/101‑1 (MQ=21)
                                          aaGATCGGCGCGGCGCTGACGCGTCGGGCCGAGCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  1:2685618/101‑1 (MQ=16)
                                          aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  1:2832748/101‑1 (MQ=21)
                                          aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  2:2377491/101‑1 (MQ=21)
                                           aGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           <  1:2532523/101‑1 (MQ=21)
                                            gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          >  2:2082422/1‑101 (MQ=21)
                                             aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAg                                         <  1:4743727/101‑1 (MQ=21)
                                              tCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATGAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAgg                                        <  1:3698639/101‑1 (MQ=21)
                                               cggcgcggcgCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGc                                       <  1:1152109/101‑1 (MQ=21)
                                                ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      >  1:4485568/1‑101 (MQ=21)
                                                 gcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGccc                                     <  2:1409247/101‑1 (MQ=21)
                                                  cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:1525965/101‑1 (MQ=21)
                                                   gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   <  1:154021/101‑1 (MQ=21)
                                                   gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   <  2:1996771/101‑1 (MQ=21)
                                                    cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  2:552187/101‑1 (MQ=21)
                                                    cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  >  1:425556/1‑101 (MQ=21)
                                                    cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  1:1323845/101‑1 (MQ=21)
                                                     ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  2:4457543/101‑1 (MQ=21)
                                                     ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  1:1719181/101‑1 (MQ=21)
                                                     ggcccTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  2:412470/101‑1 (MQ=21)
                                                      gcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAg                                >  1:20581/1‑101 (MQ=21)
                                                        gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  2:3734980/1‑101 (MQ=21)
                                                         cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             >  1:4725210/1‑101 (MQ=21)
                                                          tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            >  2:2729124/1‑101 (MQ=21)
                                                          tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            >  1:1975941/1‑101 (MQ=21)
                                                           gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  1:2804230/1‑101 (MQ=21)
                                                           gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  2:3507719/101‑1 (MQ=21)
                                                           gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  1:1495817/1‑101 (MQ=21)
                                                            aaGCGTCTGGTCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  2:49887/1‑101 (MQ=21)
                                                            aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          <  1:1603779/101‑1 (MQ=21)
                                                            aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          <  2:3680943/101‑1 (MQ=21)
                                                            aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  1:464779/1‑101 (MQ=21)
                                                             aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  2:2595886/101‑1 (MQ=21)
                                                             aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  1:765308/101‑1 (MQ=21)
                                                             aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  2:841587/101‑1 (MQ=21)
                                                             aGCGTCGGCCCGAATTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  1:4096693/101‑1 (MQ=16)
                                                              gCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCtt                        <  1:782722/101‑1 (MQ=21)
                                                               cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  1:1275997/101‑1 (MQ=21)
                                                               cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  1:3778964/101‑1 (MQ=21)
                                                                gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATATAGGCCCGGAAGCCTCCCTTTa                      <  1:2696145/101‑1 (MQ=21)
                                                                gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTa                      <  2:2271561/101‑1 (MQ=21)
                                                                 tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     <  2:2806428/101‑1 (MQ=21)
                                                                  cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    >  1:3362292/1‑101 (MQ=21)
                                                                   tGGCCGAACTCGGATAAAGGAAGAAGGGGGGATCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATcc                   >  1:4213622/1‑100 (MQ=21)
                                                                    ggCCGAACTCGGATAAAGGAAGAAGGGGGGGTCT.GCCCCCCGCTTCCCTCCCCCCGGGGTTTTTTTGGGAAGATAAAGGACAGGAAGCCTCTCTTTTTCtt                  >  1:3299210/1‑101 (MQ=255)
                                                                    ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  2:517566/101‑1 (MQ=21)
                                                                    ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGACCGGAAGCCTCCTCTTATCtt                  >  1:1852143/1‑101 (MQ=16)
                                                                     gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 >  2:1677188/1‑101 (MQ=21)
                                                                       cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               >  1:1024310/1‑101 (MQ=21)
                                                                        gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  2:2730375/1‑101 (MQ=21)
                                                                        gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  2:2543362/1‑101 (MQ=21)
                                                                        gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              <  1:2300864/101‑1 (MQ=21)
                                                                        gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  2:1056777/1‑101 (MQ=21)
                                                                         aaCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             >  2:1313813/1‑101 (MQ=21)
                                                                          acgcGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            <  1:3607280/98‑1 (MQ=21)
                                                                          aCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGACGGa            >  2:744205/1‑101 (MQ=21)
                                                                           cTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaa           <  2:934099/101‑1 (MQ=21)
                                                                             cGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         <  2:209967/101‑1 (MQ=21)
                                                                             cGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         >  2:337189/1‑101 (MQ=21)
                                                                              ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  1:486316/101‑1 (MQ=21)
                                                                              ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:4181980/101‑1 (MQ=21)
                                                                              ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:1976641/101‑1 (MQ=21)
                                                                              ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAACATAAAGGCCCCGAAGCCACCCTTTATCTTGCCGGAAata        >  2:3756175/1‑101 (MQ=16)
                                                                               gATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  1:2015319/101‑1 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      >  2:2329835/1‑101 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:43528/101‑1 (MQ=21)
                                                                                aTAAAGGAAGAAGGGGGCCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  1:1057524/101‑1 (MQ=21)
                                                                                  aaaGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  2:2237684/1‑101 (MQ=21)
                                                                                   aaGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   >  1:328758/1‑101 (MQ=21)
                                                                                   aaGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  1:4289655/101‑1 (MQ=21)
                                                                                   aaGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  1:4344193/101‑1 (MQ=21)
                                                                                   aaGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  2:4448599/101‑1 (MQ=21)
                                                                                   aaGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  1:715327/101‑1 (MQ=21)
                                                                                   aaGGAAGAAGGGGGGCTCT.GCCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  2:3683174/101‑1 (MQ=21)
                                                                                    aGGAAGAAGGGGGGCTCTGCCCCCCGCTTC.GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGCAGCCTCACTTTATCTTGACGGAAATATCCCaa  >  1:2013389/1‑101 (MQ=16)
                                                                                    aGGAAGAAGGGGGGCTCTGCCCCCCGCTTC.GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCaa  <  1:4405997/101‑1 (MQ=21)
                                                                                                     |                                                                                    
GAAGCCCGCGAAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..CCGCGCTTGTCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530839‑531022

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG