Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	900,802	+CC	100%	intergenic (‑131/‑156)	ldh ← / → ftsW	L‑lactate dehydrogenase/cell division protein FtsW

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	900,802	1	.→C	100.0%	134.7	57	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (52/4):  ref base (0/1):  total (52/5)
*	NC_014034	900,802	2	.→C	100.0%	134.4	57	intergenic (‑131/‑156)	ldh/ftsW	L‑lactate dehydrogenase/cell division protein FtsW
Reads supporting (aligned to +/- strand):  new base (52/4):  ref base (0/1):  total (52/5)

AACCCCGTTTCCG.CGCGG.C.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GC...CGCGGA..G..CC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGAGG  >  NC_014034/900725‑900876                                                                                             ||                                                                             aaCCCCGTTTCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCg                                                            >  1:1246147/1‑101 (MQ=16) aaCCCCGTTTCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCg                                                            >  2:4644356/1‑101 (MQ=16) aaCCCCGTTTCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCg                                                            >  2:1392533/1‑101 (MQ=16)  cccccGTTCCCGCCGCGG.CGGCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATCCGCC...GGGGGGTGGGGG...GGCGGA..GCCCC..CGCGGGTCGCCCCgg                                                           <  2:1028604/100‑1 (MQ=9)      cGTTTCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCt                                                       >  2:1050425/1‑101 (MQ=16)      cGTTTCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGGCGCCCCGGCCCt                                                       >  1:1234215/1‑101 (MQ=16)         ttCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGcc                                                    >  1:151701/1‑101 (MQ=16)         ttCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGcc                                                    >  2:4447180/1‑101 (MQ=16)         ttCCGCCGCGGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGcc                                                    >  1:3963653/1‑101 (MQ=16)          tccgccgcgGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCg                                                   >  1:4615472/6‑101 (MQ=16)           ccgccgcgGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCgg                                                  >  1:2187498/5‑101 (MQ=18)            cgccgcgGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGGCGCCCCGGCCCTGCCggg                                                 >  2:980625/4‑101 (MQ=16)             gccgcgGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCCGCCgggg                                                >  1:1607838/3‑101 (MQ=16)             cccgcgGCC.GCCTCTTTGCCGCAGCCCCGTTTTTCCTTGGTGCAAATCCGCCGGGGGGGGGG...GG...GGCGGACCC..CC..CGCGGGTCGCCCCGGCCCTGCCgggg                                                <  1:3663624/99‑1 (MQ=9)               cgcgccc.gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCg                                              <  2:386898/95‑1 (MQ=18)               cgcgGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGGGGA..GCCCC..CGCGGGTGGCCCCGGCCCAGCCgggcgg                                              >  2:3743627/1‑98 (MQ=16)               cgcgGCC.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCg                                              >  1:2150772/1‑101 (MQ=18)                  ggcc.gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                           >  2:1716070/4‑101 (MQ=18)                  ggcc.gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                           >  1:2755617/4‑101 (MQ=18)                  ggcc.gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGaaa                                           >  1:225394/4‑101 (MQ=18)                    cc.gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGc                                         >  1:547594/2‑101 (MQ=21)                     c.gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGcc                                        >  1:2734579/1‑101 (MQ=21)                     c.gccgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGcc                                        >  2:3814756/1‑101 (MQ=21)                        ccgcTTTGCCGCAGACCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGC...GCGGGGGCGGA..GCACC..CGCGGGACGGCCCGGGCCAACCGGGGCGAAAGcccc                                      >  2:4519412/1‑101 (MQ=255)                         cgcTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccc                                     >  1:3841648/1‑101 (MQ=18)                           cTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGccccccg                                   >  2:1715410/1‑99 (MQ=18)                           cTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgc                                   >  1:2750662/1‑101 (MQ=18)                           cTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgc                                   >  2:3644434/1‑101 (MQ=18)                           cTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgc                                   >  1:2131502/1‑101 (MQ=18)                            tttGCGGCAGCCCCGGTTTTCCGTGGTGCAAATCCGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcg                                  <  2:1404326/101‑1 (MQ=17)                            tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGACAGCCCCcgcg                                  >  2:215370/1‑101 (MQ=18)                            tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcg                                  >  2:1400021/1‑101 (MQ=18)                            tttGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGCCGCCCCGGCCCTGCCGGGGCGAAAGCCCCcgcg                                  >  1:3662999/1‑101 (MQ=21)                                 cGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGcgccg                             >  1:1356979/1‑101 (MQ=18)                                   cAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCcgcg                           >  2:1184926/1‑101 (MQ=18)                                    aGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCgg                          >  1:1540687/1‑101 (MQ=18)                                    aGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCgg                          >  1:4304590/1‑101 (MQ=18)                                      ccccGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGcc                        >  2:1503865/1‑101 (MQ=18)                                      ccccGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGcc                        >  2:818692/1‑101 (MQ=18)                                       cccGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGccc                       <  2:2115369/101‑1 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                    >  1:2880344/1‑101 (MQ=18)                                          ggTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGc                    >  1:4238575/1‑101 (MQ=18)                                           gTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGNGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                   >  1:447510/1‑101 (MQ=18)                                           gTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                   >  2:2765885/1‑101 (MQ=18)                                           gTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGGCGCCCCCGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                   >  2:2733752/1‑101 (MQ=21)                                           gTTTTCCTTGGTGCAAAGGCGCC...CGGGGGT...GCGGGGGCGGA..G..CCCGCGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCa                   <  2:536420/101‑1 (MQ=18)                                             tttCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaca                 >  1:1609749/1‑99 (MQ=18)                                             tttCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaa                 >  1:3930836/1‑101 (MQ=18)                                              ttCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaaa                >  1:53412/1‑101 (MQ=18)                                              ttCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCaaaa                >  2:1176451/1‑101 (MQ=18)                                               tCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg               >  1:1607128/1‑101 (MQ=18)                                               tCCTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAg               >  2:4331853/1‑101 (MQ=18)                                                 cTTTGTGCAAATACCCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCCGCCCCGCCGAGGCGAAAGCCCCCGCCCGCCGCGGCCCGGCAAAAGct             >  2:4695416/1‑101 (MQ=12)                                                 cTTGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGct             >  2:1264473/1‑101 (MQ=18)                                                   tGGTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCCGCCCTGCCGGGGCGAAACCCCCCGCGCGCCGCCGCCCGGCAAAAGctct           >  2:1891075/1‑101 (MQ=17)                                                     gTGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGACGAACGCCCCAGCGCACCCCGGCCCGCCACAAGATCTgg         >  2:4117420/1‑101 (MQ=9)                                                      tGCAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGt        >  2:1677527/1‑101 (MQ=18)                                                        cAAATACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCa      >  2:3510585/1‑101 (MQ=18)                                                         aaaTACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCag     >  2:3340885/1‑101 (MQ=18)                                                         aaaTACGCC...CGGGGGT...GCGGGGGAGGA..GCCCC..CGCGGGTCGGCCCGGCGCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCag     >  2:1543562/1‑101 (MQ=21)                                                          aaTACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCaga    >  2:4034924/1‑101 (MQ=18)                                                            tACGCC...CGGGGGT...GCGGGGGCGGA..GCCCC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGAgg  >  1:3699885/1‑101 (MQ=21)                                                                                             ||                                                                             AACCCCGTTTCCG.CGCGG.C.GCCGCTTTGCCGCAGCCCCGGTTTTCCTTGGTGCAAATACGCC...CGGGGGT...GC...CGCGGA..G..CC..CGCGGGTCGCCCCGGCCCTGCCGGGGCGAAAGCCCCCGCGCGCCGCGGCCCGGCAAAAGCTCTGGTCAGAGG  >  NC_014034/900725‑900876 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG