Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 925,909 +C 91.5% intergenic (+162/+12) RCAP_rcc00838 → / ← glcB DnaJ domain‑containing protein/malate synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034925,9091.→C91.5% 45.1 ‑0.8 ‑1.8201intergenic (+162/+12)RCAP_rcc00838/glcBDnaJ domain‑containing protein/malate synthase
Reads supporting (aligned to +/- strand):  new base (109/74):  ref base (8/9):  total (118/83)
Fisher's exact test strand distribution p-value = 4.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.61e-02

Only 100 of 222 total aligned reads displayed.

ACCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.CCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAA  >  NC_014034/925809‑926004
                                                                                                       |                                                                                                
aCCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.g.cg                                                                                                   <  1:2970693/101‑1 (MQ=255)
 ccccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.g.cg.c                                                                                                 >  1:1360216/1‑101 (MQ=255)
  cccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.cc                                                                                                <  2:3136985/101‑1 (MQ=255)
  cccccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.cc                                                                                                >  1:1557287/1‑101 (MQ=255)
     ccGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.ccccc                                                                                             <  1:663572/101‑1 (MQ=255)
       gggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.ccccc.cc                                                                                          >  1:4209296/1‑101 (MQ=255)
       gggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.ccccc.cc                                                                                          >  1:775729/1‑101 (MQ=255)
       gggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.ccccc.cc                                                                                          >  2:2126316/1‑101 (MQ=255)
        ggATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.ccccc.ccc                                                                                         <  1:4439881/101‑2 (MQ=255)
        ggATATTGGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.ccccc.ccc                                                                                         <  1:3557963/101‑2 (MQ=255)
         gATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.ccccc.ccct                                                                                        <  2:2663887/101‑3 (MQ=255)
          atatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.CCCCC.CCctt                                                                                       <  2:1361227/101‑1 (MQ=255)
          atatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.CCCCC.CCctt                                                                                       <  2:625520/101‑1 (MQ=255)
          atatTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.CCCCC.CCctt                                                                                       <  1:4356260/101‑1 (MQ=255)
            ttttGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTGATCTTGCCCCAAATATCCGGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.CCCCC.CCctttc                                                                                     <  1:530093/100‑3 (MQ=21)
             tttGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCt                                                                                     >  2:20730/1‑101 (MQ=255)
             ttgggggCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCt                                                                                     <  2:2655424/98‑1 (MQ=255)
              ttGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCtt                                                                                    <  2:2381549/101‑1 (MQ=255)
                   gCAAGTTAAAGGCGGCCCGTTTTCCCCTTTCATCTTGCCCCAAATACCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCt                                                                               <  1:862352/101‑1 (MQ=14)
                   gCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCt                                                                               <  1:1246585/101‑1 (MQ=255)
                    cAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCtt                                                                              <  1:2147358/101‑1 (MQ=255)
                    cAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGAGCA.G.CGCCCCCC.CCTTTCTTATGCtt                                                                              >  1:3986187/1‑101 (MQ=255)
                     aaGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCttt                                                                             >  1:996402/1‑101 (MQ=255)
                        gTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTggc                                                                          <  2:1564931/101‑1 (MQ=255)
                         tGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTggcg                                                                         >  2:4522095/1‑101 (MQ=255)
                          gAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTggcgg                                                                        >  2:1916700/1‑101 (MQ=255)
                           aaaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTggcggc                                                                       >  2:1480242/1‑101 (MQ=255)
                           aaaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTggcgac                                                                       >  2:4720155/1‑99 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGcc                                                                      <  1:1883516/101‑1 (MQ=255)
                            aaGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGcc                                                                      >  1:4571015/1‑101 (MQ=255)
                              gCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCtt                                                                    >  1:1810834/1‑101 (MQ=255)
                                aGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCa                                                                  >  2:1262717/1‑101 (MQ=255)
                                aGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCa                                                                  >  2:4115210/1‑101 (MQ=255)
                                  gCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCAcc                                                                <  2:3746729/101‑1 (MQ=255)
                                    cGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTg                                                              >  1:2417192/1‑101 (MQ=255)
                                    cGCTTTCCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTg                                                              <  2:2439540/101‑1 (MQ=255)
                                      cTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTgcg                                                            >  2:519283/1‑101 (MQ=255)
                                       tttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTgcgc                                                           >  2:688557/1‑101 (MQ=255)
                                       tttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTgcgc                                                           >  2:3509735/1‑101 (MQ=255)
                                        ttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGcc                                                          >  1:1138988/1‑101 (MQ=255)
                                        ttGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGcc                                                          >  1:3297231/1‑101 (MQ=255)
                                         tGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGccc                                                         >  2:1425905/1‑101 (MQ=255)
                                         tGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGccc                                                         >  1:3513341/1‑101 (MQ=255)
                                           cccTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCgg                                                       <  2:2022995/101‑1 (MQ=255)
                                            ccTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgc                                                      >  1:1107707/1‑101 (MQ=255)
                                            ccTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgc                                                      <  2:3828569/101‑1 (MQ=255)
                                            ccTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgc                                                      <  1:3615494/101‑1 (MQ=255)
                                             cTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGgcg                                                     <  1:4477065/101‑1 (MQ=255)
                                                  ctCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGt                                                <  2:3586013/100‑1 (MQ=255)
                                                  aTCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGt                                                <  2:3942993/101‑1 (MQ=255)
                                                   tCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTgggg                                               >  2:1835037/1‑99 (MQ=255)
                                                   tCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTg                                               >  1:166263/1‑101 (MQ=255)
                                                   tCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTg                                               <  2:593081/101‑1 (MQ=255)
                                                    cTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCC.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGGGCCCGGCGCTGGTgc                                              >  1:4303556/1‑101 (MQ=25)
                                                    cTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgc                                              <  2:254907/101‑1 (MQ=255)
                                                    cTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgc                                              >  1:633650/1‑101 (MQ=255)
                                                    cTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.CCCCCACCTTTCTTATGCTTTGGCGGGCTTCACCTGCTCCCGGCGCTGGGgc                                              >  2:4649696/1‑101 (MQ=21)
                                                     ttGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgca                                             <  1:150343/101‑1 (MQ=255)
                                                     ttGCCCCAAATAACCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgca                                             >  1:2565567/1‑101 (MQ=255)
                                                      tGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgcag                                            <  1:3204967/101‑1 (MQ=255)
                                                       gCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTgcagc                                           <  1:1948739/101‑1 (MQ=255)
                                                          ccAAATCTCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCAcc                                        <  1:1710908/101‑1 (MQ=255)
                                                          caaaaTACCCGGGGGGGGGTCCGCTGGCGGACGGGGCGCA.GCCC.CCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCAcc                                        <  2:4570762/99‑1 (MQ=255)
                                                            aaaTATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCAcaga                                      >  2:1717401/1‑98 (MQ=255)
                                                             aaTATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGc                                     >  1:3364371/1‑101 (MQ=255)
                                                              atatCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCt                                    >  1:1065289/1‑101 (MQ=255)
                                                                atCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCg                                  >  2:3871481/1‑101 (MQ=255)
                                                                 tCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCCGCACCGGCTccg                                 >  1:516857/1‑99 (MQ=255)
                                                                 tCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCgg                                 >  1:932616/1‑101 (MQ=255)
                                                                  cccGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCggg                                >  2:3214342/1‑100 (MQ=255)
                                                                  cccGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGt                                >  2:1977291/1‑101 (MQ=255)
                                                                  cccGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGGGCAGCACCGGCTCGGt                                >  2:2705765/1‑101 (MQ=255)
                                                                   ccGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGta                               >  2:28012/1‑101 (MQ=255)
                                                                    cGGGGGGGGCCCCCTGGGGGGCGGGGGCCA.GCCC.CCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtat                              <  1:3404192/101‑1 (MQ=255)
                                                                    cGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCCGCACCGGCTCGGtat                              >  1:1423968/1‑101 (MQ=255)
                                                                    cGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtat                              >  1:2062955/1‑101 (MQ=255)
                                                                     ggggggAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtata                             <  2:3373060/101‑1 (MQ=255)
                                                                     ggggggAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGtata                             <  2:3209709/101‑1 (MQ=255)
                                                                     ggggggAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCCGgata                             >  2:347450/1‑101 (MQ=25)
                                                                         ggAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATCCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGccc                         >  1:4725267/1‑101 (MQ=255)
                                                                          gAGTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCg                        <  2:3107054/101‑1 (MQ=255)
                                                                            gTCCGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGGATAGCCCggg                      >  2:3623242/1‑99 (MQ=255)
                                                                              ccGCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCCGGCTAGCCCGGggg                    >  2:2839059/1‑100 (MQ=25)
                                                                              ccGCGTGCGGACGGGGGGCAGG.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAgg                     <  2:4054739/101‑1 (MQ=38)
                                                                                gCTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATGGCCCGAGGACt                  <  2:864184/101‑1 (MQ=255)
                                                                                 cTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGGCTg                 >  1:3965388/1‑101 (MQ=255)
                                                                                 cTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTg                 >  1:1454868/1‑101 (MQ=255)
                                                                                 cTTGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTg                 <  2:3982062/101‑1 (MQ=255)
                                                                                   tGCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCCAGGACTGGa               >  2:4439657/1‑101 (MQ=255)
                                                                                    gCGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCCGGATAGCCCGAgggccggct              >  2:2256010/1‑94 (MQ=37)
                                                                                     cGGACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATc             <  2:2238198/101‑1 (MQ=255)
                                                                                      ggACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGCCTGGGTcc            >  1:4457794/1‑101 (MQ=38)
                                                                                      ggACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATcc            <  2:4287710/101‑1 (MQ=255)
                                                                                      ggACGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATcc            <  1:2231199/101‑1 (MQ=255)
                                                                                        aCGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCgg          <  1:405056/101‑1 (MQ=255)
                                                                                         cGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCggg         >  1:3770558/1‑101 (MQ=255)
                                                                                         cGGGGGGCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCggg         >  1:1801770/1‑101 (MQ=255)
                                                                                          ggggggCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGc        >  1:1284415/1‑101 (MQ=255)
                                                                                              ggCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAgg    <  2:2917225/101‑1 (MQ=255)
                                                                                               gCA.G.CGCCCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGa   >  1:3125630/1‑101 (MQ=255)
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ACCCCCCGGGATATTTGGGGCAAGGTGAAAGCAGGCCGCTTTGCCCTTTCATCTTGCCCCAAATATCCCGGGGGGAGTCCGCTTGCGGACGGGGGGCA.G.CG.CCCCC.CCTTTCTTATGCTTTGGCGGCCTTCACCTGCGCCCGGCGCTGGTGCAGCACCGGCTCGGTATAGCCCGAGGACTGGATCCGGGCGAGGAA  >  NC_014034/925809‑926004

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG