Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,398,0311.→T 0.7% 0.5 ‑1.6 ‑0.4285intergenic (+335/+1563)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/0):  ref base (127/156):  total (129/156)
Fisher's exact test strand distribution p-value = 2.04e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.33e-01

Only 100 of 312 total aligned reads displayed.

AAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATT  >  NC_014034/1397931‑1398131
                                                                                                     |                                                                                                      
 aaCTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..a                                                                                                      <  2:1249616/101‑1 (MQ=255)
   ctctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGc                                                                                                    <  1:377646/101‑1 (MQ=255)
     ctATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCa.a                                                                                                 >  1:1235750/1‑101 (MQ=255)
      tATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.Ag                                                                                                <  2:660101/101‑1 (MQ=255)
        tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTc                                                                                              <  1:2795177/101‑1 (MQ=255)
        tGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTc                                                                                              >  2:375502/1‑101 (MQ=255)
           gaggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCgat                                                                                           >  2:553714/1‑99 (MQ=255)
            aggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTc                                                                                          <  1:4681856/101‑1 (MQ=255)
             ggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcc                                                                                         >  1:4334920/1‑101 (MQ=255)
             ggctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcc                                                                                         >  1:707307/1‑101 (MQ=255)
               ctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccc                                                                                       >  2:122082/1‑101 (MQ=255)
               ctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccc                                                                                       >  1:1817712/1‑101 (MQ=255)
               ctagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAACGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.CGTCGCTcccc                                                                                       >  2:3280321/1‑101 (MQ=255)
                tagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccccc                                                                                      >  2:3704112/1‑101 (MQ=255)
                tagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccccc                                                                                      >  1:112108/1‑101 (MQ=255)
                tagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccccc                                                                                      >  1:1728001/1‑101 (MQ=255)
                tagggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTccccc                                                                                      >  1:3527552/1‑101 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                                                     >  1:1347615/1‑101 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                                                     <  2:2491669/101‑1 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTcccccc                                                                                     >  2:1692058/1‑101 (MQ=255)
                 agggaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCGcccccc                                                                                     >  2:1263536/1‑101 (MQ=255)
                    gaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgcg                                                                                  >  1:2877496/1‑101 (MQ=255)
                    gaggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCcgcg                                                                                  >  1:3314752/1‑101 (MQ=255)
                      ggctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCggg                                                                                >  1:675332/1‑101 (MQ=255)
                        ctaCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCggggg                                                                              >  1:213707/1‑101 (MQ=255)
                         taCGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGc                                                                             >  2:4579060/1‑101 (MQ=255)
                             cACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTgg                                                                         >  2:2009497/1‑101 (MQ=255)
                             cACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTgg                                                                         >  2:2360063/1‑101 (MQ=255)
                                  tgcggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAA.CGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  1:4621105/7‑101 (MQ=2)
                                   cAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                                                   <  1:1692163/101‑1 (MQ=255)
                                   cAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                                                   >  1:4127131/1‑101 (MQ=255)
                                   cAAAAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                                                   <  1:197684/101‑1 (MQ=255)
                                   cAAAAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTa                                                                   <  1:1004438/101‑1 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                                                  >  2:3964727/1‑101 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTaa                                                                  <  2:3988726/101‑1 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCCCGGGGGCGTGGATCGTaa                                                                  >  2:1345746/1‑101 (MQ=255)
                                    aaaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGACGCTCCCCCCGCGGGGGCGGGGATCGTaa                                                                  >  1:2349680/1‑101 (MQ=255)
                                     aaaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAg                                                                 >  1:1413958/1‑101 (MQ=255)
                                      aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGc                                                                <  2:808568/101‑1 (MQ=255)
                                      aaGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGc                                                                >  1:2975490/1‑101 (MQ=255)
                                       aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCg                                                               >  2:3464419/1‑101 (MQ=255)
                                       aGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCg                                                               <  1:3988161/101‑1 (MQ=255)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAGGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGt                                                             >  1:4059987/1‑101 (MQ=255)
                                         tCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGt                                                             <  2:3814309/101‑1 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTg                                                            >  2:2210810/1‑101 (MQ=255)
                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGGGGATCGTAAGCGGTg                                                            >  1:1995637/1‑101 (MQ=255)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                         >  2:3115462/1‑101 (MQ=255)
                                             cccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCAt                                                         <  1:2942188/100‑1 (MQ=255)
                                              ccccccGCGGGGGGGGGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                        <  1:2446308/101‑1 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                        >  1:3781159/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGAGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATc                                                        <  2:4067196/101‑1 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCg                                                       >  1:3727614/1‑101 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCg                                                       <  2:2978727/101‑1 (MQ=255)
                                                  ccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGcc                                                    <  1:4501617/101‑1 (MQ=255)
                                                  ccGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGcc                                                    >  2:2753586/1‑101 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGa                                                 <  1:21099/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                <  2:3148480/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGAt                                                <  1:3814858/101‑1 (MQ=255)
                                                       ggggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                                               <  1:278228/101‑1 (MQ=255)
                                                       gggcCGTGGAGCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATa                                               <  2:3124521/101‑1 (MQ=255)
                                                        gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaa                                              <  1:816742/101‑1 (MQ=255)
                                                        gggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaa                                              <  2:1817603/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaaa                                             <  1:1503617/101‑1 (MQ=255)
                                                         ggCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATaaa                                             <  1:3664250/101‑1 (MQ=255)
                                                            gTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAccc                                          <  2:1485062/101‑1 (MQ=255)
                                                             tGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAcccc                                         <  2:1087969/101‑1 (MQ=255)
                                                              ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                        >  1:4278084/1‑101 (MQ=255)
                                                              ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                        >  1:231662/1‑101 (MQ=255)
                                                              ggATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCg                                        <  1:305831/101‑1 (MQ=255)
                                                               gATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGa                                       <  2:1877525/101‑1 (MQ=255)
                                                                   gAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGAtttt                                   <  2:3590867/101‑1 (MQ=255)
                                                                    aaaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..GGCA.AGTCGCCCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTc                                  <  2:3674882/101‑1 (MQ=255)
                                                                     aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                 >  2:4191813/1‑101 (MQ=255)
                                                                     aaCCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCg                                 >  2:3516188/1‑101 (MQ=255)
                                                                        cgccgcGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGcc                              <  1:23859/101‑1 (MQ=255)
                                                                            gcgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                          <  2:4336760/101‑1 (MQ=255)
                                                                            gcgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                          <  2:4283278/101‑1 (MQ=255)
                                                                            gcgcAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTg                          <  1:4550762/101‑1 (MQ=255)
                                                                               cAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAgg                       <  2:2850750/101‑1 (MQ=255)
                                                                                  aaaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGa                    <  2:1158595/101‑1 (MQ=255)
                                                                                   aaGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGaa                   >  1:1924931/1‑101 (MQ=255)
                                                                                        cGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGAtt              <  1:553765/101‑1 (MQ=255)
                                                                                        cGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGAtt              >  2:2644056/1‑101 (MQ=255)
                                                                                          ccAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt            >  1:947914/1‑101 (MQ=255)
                                                                                          ccAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt            <  2:2417983/101‑1 (MQ=255)
                                                                                          ccAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCt            <  1:4428860/101‑1 (MQ=255)
                                                                                           cAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCtt           <  1:3951460/101‑1 (MQ=255)
                                                                                              gaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAGCCCGACTTTCGGCCCCTTAGGGGGAAGATTCTTGGc        >  1:4246355/1‑101 (MQ=38)
                                                                                              gaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGc        >  1:3618078/1‑101 (MQ=255)
                                                                                              gaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGc        <  1:201900/101‑1 (MQ=255)
                                                                                              gaagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAAACCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGc        >  1:2521392/1‑101 (MQ=255)
                                                                                               aagCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGGAAGATTCTTGGca       >  2:616376/1‑101 (MQ=255)
                                                                                                agCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcac      <  2:2282528/101‑1 (MQ=255)
                                                                                                agCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcac      <  2:4477447/101‑1 (MQ=255)
                                                                                                agCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcac      <  2:213682/101‑1 (MQ=255)
                                                                                                 gCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGcaca     <  2:707243/101‑1 (MQ=255)
                                                                                                   gg..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAt   >  1:99068/1‑101 (MQ=255)
                                                                                                    g..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAtt  >  2:2824354/1‑101 (MQ=255)
                                                                                                    g..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAtt  <  1:4468551/101‑1 (MQ=255)
                                                                                                    g..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAAtt  <  2:3314550/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
AAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGCCGCGCAAAAAGCCCGCCAAGAAGCGG..AGCA.AGTCGCTCCCCCCGCGGGGGCGTGGATCGTAAGCGGTGCATCGGCCCGATAAACCCCGATTTTCGGCCCCTGAGGGGAAAGATTCTTGGCACAATT  >  NC_014034/1397931‑1398131

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG