Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,3980A→T 1.4% 1.6 ‑0.5 ‑0.0287intergenic (‑177/‑781)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (1/3):  ref base (123/160):  total (124/163)
Fisher's exact test strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.92e-01

Only 100 of 300 total aligned reads displayed.

CTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGG  >  NC_014034/1434298‑1434498
                                                                                                        |                                                                                                        
  tACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACg                                                                                                        <  1:4266739/101‑1 (MQ=255)
     cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                                    >  1:4485369/1‑101 (MQ=255)
     cGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                                    <  1:3816984/101‑1 (MQ=255)
     cGTGGATCGAAACAGGCTGCTTTGGGCGGAACAACTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCt                                                                                                    >  2:1222957/1‑101 (MQ=255)
      gTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTg                                                                                                   <  1:2306413/101‑1 (MQ=255)
       tGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.c                                                                                                 >  1:2640395/1‑101 (MQ=255)
         gATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGt                                                                                               >  2:2082344/1‑101 (MQ=255)
          aTCGGAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTc                                                                                              <  2:1424211/101‑1 (MQ=255)
           tCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCa                                                                                             <  2:1150712/101‑1 (MQ=255)
             gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                           >  2:523855/1‑101 (MQ=255)
             gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                           <  1:569680/101‑1 (MQ=255)
             gAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                           >  1:2930792/1‑101 (MQ=255)
             gAAACAGGCTGCTTTGGGCGGAACACCTGATACTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAc                                                                                           >  1:4206891/1‑101 (MQ=255)
              aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAAcc                                                                                          >  1:3439144/1‑101 (MQ=255)
                 cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..g                                                                                     <  2:1972907/101‑1 (MQ=255)
                  aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCACCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GGTG.CGGCAACCTT..gc                                                                                    >  2:2423073/1‑100 (MQ=255)
                   ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTc                                                                                   <  1:3139922/101‑1 (MQ=255)
                   agcttgtttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTc                                                                                   <  1:1551066/95‑1 (MQ=255)
                    gctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCg                                                                                  >  2:1725847/1‑101 (MQ=255)
                      tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCt                                                                                <  2:1605897/101‑1 (MQ=255)
                           tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                           >  1:2386248/1‑101 (MQ=255)
                           tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTccccc                                                                           >  1:491401/1‑101 (MQ=255)
                            gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                          <  2:866143/101‑1 (MQ=255)
                            gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTcccccc                                                                          <  1:2035998/101‑1 (MQ=255)
                               cGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCcgcg                                                                       <  2:4394816/101‑1 (MQ=255)
                                 gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCggg                                                                     >  1:1113275/1‑101 (MQ=255)
                                   acacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCggggg                                                                   <  1:1725952/101‑1 (MQ=255)
                                    cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                  <  1:3080321/101‑1 (MQ=255)
                                        tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                              <  2:2941961/101‑1 (MQ=255)
                                        tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:2469923/101‑1 (MQ=255)
                                        tGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCCCGGGGGCGGgg                                                              >  1:3666944/1‑101 (MQ=255)
                                         gATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCgggttcgtctg                                                             <  2:1093965/101‑9 (MQ=255)
                                          aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  2:620265/1‑101 (MQ=255)
                                            ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  1:1837975/1‑101 (MQ=255)
                                             ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:2513487/94‑1 (MQ=2)
                                             ctgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  1:1252661/101‑1 (MQ=255)
                                              tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        >  2:4054996/1‑101 (MQ=255)
                                              tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        >  1:3250021/1‑101 (MQ=255)
                                              gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                           >  2:4640334/7‑101 (MQ=2)
                                               gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       >  1:1241780/1‑101 (MQ=255)
                                                tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  1:4712853/5‑101 (MQ=2)
                                                ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      <  2:2238436/101‑1 (MQ=255)
                                                ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      >  1:4693355/1‑101 (MQ=255)
                                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                     >  2:1839549/1‑101 (MQ=255)
                                                 tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                     >  2:2066590/1‑101 (MQ=255)
                                                 ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCCGCTGACACCGCCAACAAA.TATGTGGTCAACACT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  1:1604566/98‑1 (MQ=2)
                                                 atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  1:2703893/98‑1 (MQ=2)
                                                  tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  2:3130573/99‑1 (MQ=2)
                                                   cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                      >  2:3387093/2‑101 (MQ=2)
                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacg                                                     >  2:3917046/1‑100 (MQ=2)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                              <  2:3600341/101‑1 (MQ=255)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                             >  2:275920/1‑101 (MQ=255)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                             >  1:289408/1‑101 (MQ=255)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                             >  2:3031940/1‑101 (MQ=255)
                                                         cccccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.ATTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                             <  1:3320824/100‑1 (MQ=255)
                                                           cccccGCGGGGGCGTGGATCGAAACGGGTCGAAG.ACCTG.CTTGACGCGCTG.GATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                              <  1:4523520/101‑9 (MQ=2)
                                                           cccccGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCAt                                           <  1:320937/101‑1 (MQ=255)
                                                            cgccGCGGGGGCGGGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                          <  2:1379618/99‑1 (MQ=255)
                                                            ccccGCGGGGGCGTGGATCGAAAC..TTGTCTG.ACGCG.CTCATCA.GC.A.CGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                           <  2:3832343/101‑8 (MQ=37)
                                                               cgcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                                       <  2:3438937/101‑1 (MQ=255)
                                                                gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                      <  2:1888606/101‑1 (MQ=255)
                                                                gcgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                      >  1:3866284/1‑101 (MQ=255)
                                                                 cgGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                     <  1:1937096/101‑1 (MQ=255)
                                                                  gggggCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCGCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                    <  1:37542/101‑1 (MQ=255)
                                                                        gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                              >  1:1757774/1‑101 (MQ=255)
                                                                        gTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                              >  1:3478624/1‑101 (MQ=255)
                                                                         tGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCCAGGGGCa                             >  2:1182921/1‑101 (MQ=255)
                                                                          ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                            >  2:2968201/1‑101 (MQ=255)
                                                                          ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                            >  1:2546965/1‑101 (MQ=255)
                                                                          ggATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                            <  2:3986570/101‑1 (MQ=255)
                                                                           gATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                           <  2:168677/101‑1 (MQ=255)
                                                                              cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTGCCTCCACCCGCGGGGGCGCGGATCGAAACACGGATTGCATGGCGCCGGGGGACAGGCtt                        >  1:798236/1‑101 (MQ=21)
                                                                              cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                        <  2:4166228/101‑1 (MQ=255)
                                                                              cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                        >  1:690576/1‑101 (MQ=255)
                                                                              cGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                        <  1:2148793/101‑1 (MQ=255)
                                                                               gAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                       <  1:1488960/101‑1 (MQ=255)
                                                                                aaaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                      >  2:4537277/1‑101 (MQ=255)
                                                                                 aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                     <  1:1005468/101‑1 (MQ=255)
                                                                                 aaC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                     <  2:2263216/101‑1 (MQ=255)
                                                                                   c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                   <  2:1532518/101‑1 (MQ=255)
                                                                                   c..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                   >  1:54977/1‑101 (MQ=255)
                                                                                       tctcCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                 <  1:3960485/101‑1 (MQ=255)
                                                                                        ctcCG.CTCTG.CTTCCCT.GCTC.CGTCAACCGT..GTCGGAGCCCGCGCGGGGGCGTGGATCGTGACACAGATTGCATGGCGCCGTGTGACAGGCTTTGCAGTcc                <  2:4676152/101‑2 (MQ=12)
                                                                                           cG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc             >  1:868489/1‑101 (MQ=255)
                                                                                            g.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGTATGGTGCCGAGGGACAGGCTTTGCAGTCGCTcc            <  1:118570/101‑1 (MQ=255)
                                                                                               tctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc          <  2:1247404/101‑1 (MQ=255)
                                                                                               tctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc          >  1:4415539/1‑101 (MQ=255)
                                                                                                ctG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc         <  2:3220895/101‑1 (MQ=255)
                                                                                                 tG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc        <  2:2475124/101‑1 (MQ=255)
                                                                                                 tG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGCCGCTcccccc        >  1:1582367/1‑101 (MQ=255)
                                                                                                  g.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg       <  1:2093642/101‑1 (MQ=255)
                                                                                                  g.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg       <  2:369299/101‑1 (MQ=255)
                                                                                                  g.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg       >  2:3677983/1‑101 (MQ=255)
                                                                                                  g.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg       <  2:1809677/101‑1 (MQ=255)
                                                                                                  g.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg       >  2:2847333/1‑101 (MQ=255)
                                                                                                    cTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgc      <  1:1306145/101‑1 (MQ=255)
                                                                                                      tCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgg    <  2:4485119/101‑1 (MQ=255)
                                                                                                       cACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggg   >  2:731123/1‑101 (MQ=255)
                                                                                                       cACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAGTGCATTGCGCCGAGGGACAGGCTTTGCAGCCTCTCCCCCCGCggg   >  1:3023429/1‑101 (MQ=14)
                                                                                                        aCG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggg  <  1:4023038/101‑1 (MQ=255)
                                                                                                        |                                                                                                        
CTTACCGTGGATCGAAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCG.CTCTG.CTTCACG.GCTG.CGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGG  >  NC_014034/1434298‑1434498

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG