Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,975	1	.→C	100.0%	5.7	5	intergenic (+248/‑257)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (0/0):  total (2/3)

AGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGG  >  NC_014034/2185879‑2186064                                                                                                    |                                                                                           agcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccc                                                                                       <  1:664908/101‑2 (MQ=37) agcGGCTTGCCCCGAAAGACTTG.TTTTGATGGCACAAGAAATACCCA.AACGCCCCCTTTCACCTTTGCTCAAAAATGCCGGGGGGACGGGGGCGGAgggcc                                                                                         >  2:1992097/1‑97 (MQ=255)  tcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..ccccc                                                                                      <  2:2030180/100‑3 (MQ=37)  gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..ccccc                                                                                      <  1:4261302/101‑3 (MQ=37)   cgtcgtGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccg                                                                                     <  2:706048/96‑4 (MQ=25)   cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccg                                                                                     <  1:3912014/101‑4 (MQ=37)       ttGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccggcgg                                                                                 >  1:4522225/1‑94 (MQ=25)        tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccggcggg                                                                                >  1:2443020/1‑93 (MQ=21)         cccccGAATGCCTTG.TTTTGATGGCACAAGAAACCCCGACCCCC.CCCCTTTCCCCTGGGCTAAAATTCCCGGGGGGTCGGGGGGCGGAG..cccccggcgggt                                                                               <  2:3418760/100‑10 (MQ=12)          ccccGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGA.ACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..cccccggcggggg                                                                              >  2:1550205/1‑91 (MQ=21)                                                   ccgcccccTTTCACCTTGGCTAAAATATCCGGGGGGTCCGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                       <  2:3631859/97‑1 (MQ=2)                                                                  ttGGCTCAAATATCCCGGGGGTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                        <  2:589166/101‑1 (MQ=9)                                                                   tGGCTCAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGGCT.CGGGCGCAGGGCGGGGGCG.GAGAACCCAGCGCCCCccaccgacccgcggcc                       >  2:457866/1‑93 (MQ=2)                                                                                     ggTCGGGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGcc     <  2:4492861/101‑1 (MQ=12)                                                                                        ccGGGGGCGGAGCCCCCGGCGGGGCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCCGCGCCCCCGACCGACCTGCGACGGCCCGTCGCCCAAAAGCCCgg  >  2:2558113/1‑101 (MQ=9)                                                                                                    |                                                                                           AGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGA.ACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGG  >  NC_014034/2185879‑2186064 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG