Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034774,6220G→T100.0% 7.6 3intergenic (‑76/+44)oppB1/oppA1oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (3/0):  ref base (0/0):  total (3/0)

CCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTC  >  NC_014034/774600‑774704
                      |                                                                                    
gcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCa        >  1:585880/6‑101 (MQ=255)
   cggcgcCCCCCCGCCCGGGTCGGACCGGCCCCGCCGGTCCGACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCCGTCGGCCACGTTCCAGTa     >  2:4697769/3‑101 (MQ=255)
      cgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCCGACCCTACGGTCTCTTATCCTGCCATCACTTCGCCCGCGACCAGTCAGCCACGTTCCAGTATTc  >  2:4263997/1‑101 (MQ=255)
                      |                                                                                    
CCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.CGA.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTC  >  NC_014034/774600‑774704

Base quality scores:  ATCG  < 3 ≤  ATCG  < 10 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 39 ≤  ATCG