Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,201,931 T→A 100% S342T (TCC→ACC)  trpB1 → tryptophan synthase subunit beta

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,201,9310T→A100.0% 815.8 223S342T (TCC→ACC) trpB1tryptophan synthase subunit beta
Reads supporting (aligned to +/- strand):  new base (111/111):  ref base (0/0):  total (111/112)

Only 100 of 234 total aligned reads displayed.

TGGAGGGGCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCTCCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCATGTGATGAA  >  NC_014034/1201833‑1202028
                                                                                                  |                                                                                                 
tGGAGGGGCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATGTGTCTCGATCAcc                                                                                                 >  1:4079838/1‑101 (MQ=255)
tGGAGGGGCATTCGATCTCGGCCGGACTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCAcc                                                                                                 >  1:2231423/1‑101 (MQ=255)
gggAGGGGCGGTCCGTGTCGGCCGGCCGTGATTCTGCGGGCATCGGGCCGGAGCATTCGTGGTTGCATGCCATCAACCGCGCCGAATATGTCTCGATCAcc                                                                                                 <  2:3843634/100‑1 (MQ=255)
   gggggCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGAt                                                                                              <  1:4256974/100‑1 (MQ=255)
   aGGGGGCTTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGAt                                                                                              <  2:714417/101‑1 (MQ=255)
   aGGGGCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGAt                                                                                              <  1:3906323/101‑1 (MQ=255)
   aGGGGCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGAt                                                                                              <  2:377554/101‑1 (MQ=255)
   aGGGGCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGAt                                                                                              <  2:3651314/101‑1 (MQ=255)
    ggggCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATg                                                                                             >  1:32016/1‑101 (MQ=255)
     gggCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGc                                                                                            >  2:353410/1‑101 (MQ=255)
      ggCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGcc                                                                                           <  1:247572/101‑1 (MQ=255)
       gCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCg                                                                                          <  1:2582395/101‑1 (MQ=255)
         aTTCGATCTCTGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAg                                                                                        >  1:386905/1‑101 (MQ=255)
         aTTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAg                                                                                        >  2:550757/1‑101 (MQ=255)
         aTTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAg                                                                                        >  2:2044251/1‑101 (MQ=255)
            cGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGgcg                                                                                     <  2:4231415/101‑1 (MQ=255)
             gATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGgcgc                                                                                    >  2:2546516/1‑101 (MQ=255)
             gATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGgcgc                                                                                    >  1:1934410/1‑101 (MQ=255)
             gATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGgcgc                                                                                    >  2:2435219/1‑101 (MQ=255)
             gATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACAGAtcggaagagcg                                                                                    >  2:3466631/1‑91 (MQ=255)
              aTCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCt                                                                                   >  1:1370193/1‑101 (MQ=255)
               tctcGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTc                                                                                  <  1:4027046/101‑1 (MQ=255)
               tctcGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTc                                                                                  >  1:4382265/1‑101 (MQ=255)
                ctcGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCg                                                                                 >  2:613249/1‑101 (MQ=255)
                ctcGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCg                                                                                 >  2:3964617/1‑101 (MQ=255)
                ctcGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCg                                                                                 >  1:514928/1‑101 (MQ=255)
                 tcGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGa                                                                                <  1:3131341/101‑1 (MQ=255)
                      cggccTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCt                                                                           >  2:2153405/1‑101 (MQ=255)
                         ccTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCTCTCACCGATGCCGAGGCGCTCGACGCCTTcc                                                                        <  1:4226717/101‑1 (MQ=255)
                         ccTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTcc                                                                        <  1:890877/101‑1 (MQ=255)
                          cTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCa                                                                       <  2:2238464/101‑1 (MQ=255)
                            tGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGt                                                                     <  1:72470/101‑1 (MQ=255)
                            tGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGt                                                                     <  2:1349143/101‑1 (MQ=255)
                            tGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGt                                                                     >  2:4345307/1‑101 (MQ=255)
                             gATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGtt                                                                    >  1:3451615/1‑101 (MQ=255)
                             gATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGtt                                                                    >  2:3324657/1‑101 (MQ=255)
                             gATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGtt                                                                    >  2:3270252/1‑101 (MQ=255)
                              aTTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTc                                                                   >  1:2623102/1‑101 (MQ=255)
                               ttATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTct                                                                  >  1:1867689/1‑101 (MQ=255)
                                tATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTctg                                                                 >  1:2489987/1‑101 (MQ=255)
                                 aTCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTctgc                                                                >  1:4002687/1‑101 (MQ=255)
                                 aTCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTctgc                                                                >  1:3399607/1‑101 (MQ=255)
                                  tCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTctgct                                                               >  1:1577481/1‑101 (MQ=255)
                                     gggCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTc                                                            <  2:3384016/101‑1 (MQ=255)
                                      ggCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCg                                                           <  2:4615415/101‑1 (MQ=255)
                                      ggCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCg                                                           >  2:4426181/1‑101 (MQ=255)
                                      ggCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCg                                                           >  2:235643/1‑101 (MQ=255)
                                       gCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCgg                                                          <  1:4605261/101‑1 (MQ=255)
                                       gCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCgg                                                          <  1:550808/101‑1 (MQ=255)
                                         aTCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCgggc                                                        >  2:860991/1‑99 (MQ=255)
                                         aTCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGAc                                                        >  2:2985620/1‑101 (MQ=255)
                                         aTCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGAc                                                        <  1:838863/101‑1 (MQ=255)
                                         aTCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGAc                                                        <  1:3011585/101‑1 (MQ=255)
                                           cGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGCCCg                                                      >  1:855531/1‑101 (MQ=255)
                                           cGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCg                                                      >  1:2041714/1‑101 (MQ=255)
                                           cGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCg                                                      <  2:2207378/101‑1 (MQ=255)
                                              gCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGCgg                                                   >  2:3136968/1‑101 (MQ=255)
                                               ccGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAggg                                                  <  1:2284716/101‑1 (MQ=255)
                                                cGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAgggg                                                 >  1:1725473/1‑101 (MQ=255)
                                                 ggAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGa                                                >  1:2898008/1‑101 (MQ=255)
                                                 ggAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGa                                                >  2:1112045/1‑101 (MQ=255)
                                                    gCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCa                                             >  2:153666/1‑101 (MQ=255)
                                                      aTTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGCCGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCAtt                                           >  2:3046997/1‑101 (MQ=255)
                                                       ttCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTc                                          >  1:1965295/1‑101 (MQ=255)
                                                       ttCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCGTCCAGTTCTGCTGTCGGACCGAGGGGGTCATTc                                          >  1:4043334/1‑101 (MQ=255)
                                                        tCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTcc                                         <  2:4718619/101‑1 (MQ=255)
                                                          gTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGGTCATTCCgg                                       >  2:1709749/1‑101 (MQ=255)
                                                            ggCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGcc                                     >  1:2431145/1‑101 (MQ=255)
                                                            ggCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGcc                                     <  2:3449021/101‑1 (MQ=255)
                                                              gtGCATGGCATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTAGACGCCTGCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCtt                                   <  2:4645653/100‑1 (MQ=255)
                                                               tGCATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTg                                  <  1:3292289/101‑1 (MQ=255)
                                                                 cATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGa                                >  2:962789/1‑101 (MQ=255)
                                                                 cATGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGa                                >  1:1452843/1‑101 (MQ=255)
                                                                  aTGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCCGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGaa                               >  1:3276969/1‑101 (MQ=255)
                                                                  aTGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGaa                               >  1:3318373/1‑101 (MQ=255)
                                                                  aTGACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGaa                               <  2:1265275/101‑1 (MQ=255)
                                                                    gACATCAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAag                             <  2:192683/101‑1 (MQ=255)
                                                                         cAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGcc                        >  2:2547605/1‑101 (MQ=255)
                                                                         cAAGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGcc                        >  2:2155592/1‑101 (MQ=255)
                                                                          aaGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCa                       <  2:3838862/101‑1 (MQ=255)
                                                                           aGCGCGCCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCAt                      <  1:3979905/101‑1 (MQ=255)
                                                                            gcgcgcCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATg                     <  2:2280143/101‑1 (MQ=255)
                                                                            gcgcgcCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATg                     <  1:2810889/101‑1 (MQ=255)
                                                                             cgcgcCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATgc                    <  1:732969/101‑1 (MQ=255)
                                                                               cgcCGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATgcgc                  >  2:3575269/1‑101 (MQ=255)
                                                                                ccgatctatGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCt                 <  1:541018/95‑1 (MQ=255)
                                                                                 ccGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTc                <  2:2924129/101‑1 (MQ=255)
                                                                                  cGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCNTNNCGGCCTTNNANAGNANNCNTGCGCTCg               >  1:2067311/1‑101 (MQ=255)
                                                                                  cGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCg               <  1:2155716/101‑1 (MQ=255)
                                                                                  cGAATATGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCg               >  1:2654741/1‑101 (MQ=255)
                                                                                      tatGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCa           <  2:4006794/101‑1 (MQ=255)
                                                                                      tatGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCa           >  1:3827381/1‑101 (MQ=255)
                                                                                       atGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCAt          <  1:2934015/101‑1 (MQ=255)
                                                                                       atGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCAt          <  1:1240995/101‑1 (MQ=255)
                                                                                        tGTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCAtg         <  1:1048146/101‑1 (MQ=255)
                                                                                         gTCTCGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCAtgt        <  2:4061340/101‑1 (MQ=255)
                                                                                            tcGATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCATGtgat     <  2:1400304/101‑1 (MQ=255)
                                                                                              gATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCATGtgatga   >  1:1025904/1‑101 (MQ=255)
                                                                                              gATCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCATGtgatga   >  2:1757394/1‑101 (MQ=255)
                                                                                               aTCACCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCATGTGATGaa  >  1:3701362/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
TGGAGGGGCATTCGATCTCGGCCGGCCTTGATTATCCGGGCATCGGGCCGGAGCATTCGTGGCTGCATGACATCAAGCGCGCCGAATATGTCTCGATCTCCGATGCCGAGGCGCTCGACGCCTTCCAGTTCTGCTGTCGGACCGAGGGGATCATTCCGGCCTTGGAAAGCAGCCATGCGCTCGCCCATGTGATGAA  >  NC_014034/1201833‑1202028

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG