Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,244,803 G→T 100% A294S (GCC→TCC)  RCAP_rcc01174 → D‑2‑hydroxyglutarate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,244,8030G→T100.0% 756.4 198A294S (GCC→TCC) RCAP_rcc01174D‑2‑hydroxyglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (101/97):  ref base (0/0):  total (101/97)

Only 100 of 205 total aligned reads displayed.

AACCGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAAGCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAATC  >  NC_014034/1244705‑1244900
                                                                                                  |                                                                                                 
aaCCGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATcc                                                                                                 >  1:4238343/1‑101 (MQ=255)
aaCCGGAGGTGCCCTGTCCCTATGCGCCCCGCCCGGTCGATCTGCTGATCGAGAGCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATcc                                                                                                 <  2:2338255/101‑1 (MQ=255)
 aCCGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGGCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATccc                                                                                                <  1:2835682/101‑1 (MQ=255)
  ccGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCt                                                                                               <  1:2442496/101‑1 (MQ=255)
   cGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTg                                                                                              >  1:113117/1‑101 (MQ=255)
      cgcTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCtt                                                                                           <  2:4497391/101‑1 (MQ=255)
      cgcTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCtt                                                                                           <  2:4638980/101‑1 (MQ=255)
        cTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGCCCACCGTGCCCGTCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTgg                                                                                         <  2:301326/101‑1 (MQ=255)
        cTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTgg                                                                                         >  2:1505695/1‑101 (MQ=255)
         tGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgc                                                                                        >  1:2968326/1‑101 (MQ=255)
          ggCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgcg                                                                                       >  1:691587/1‑101 (MQ=255)
          ggCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgcg                                                                                       >  1:3527280/1‑101 (MQ=255)
           gCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGgcgc                                                                                      >  1:1745035/1‑101 (MQ=255)
             cTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcgg                                                                                    >  1:4508465/1‑101 (MQ=255)
             cTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcgg                                                                                    >  1:762552/1‑101 (MQ=255)
              tGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCgcggc                                                                                   >  1:457171/1‑101 (MQ=255)
                tCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGa                                                                                 <  1:2665045/101‑1 (MQ=255)
                   cTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATgg                                                                              >  1:1679820/1‑101 (MQ=255)
                   cTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATgg                                                                              <  2:284406/101‑1 (MQ=255)
                    tATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGa                                                                             >  1:356200/1‑101 (MQ=255)
                    tATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGa                                                                             <  1:1990375/101‑1 (MQ=255)
                      tGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAgg                                                                           <  1:181209/101‑1 (MQ=255)
                       gcgcCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGc                                                                          >  1:563736/1‑101 (MQ=255)
                       gcgcCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGc                                                                          <  2:2141905/101‑1 (MQ=255)
                        cgccccgcccGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCg                                                                         >  1:351471/1‑101 (MQ=255)
                         gccccgcccGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCgg                                                                        >  1:2021727/1‑101 (MQ=255)
                            ccgcccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGc                                                                     <  1:4364630/101‑1 (MQ=255)
                             cgcccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCa                                                                    <  1:3779784/101‑1 (MQ=255)
                               cccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGa                                                                  <  1:3113989/101‑1 (MQ=255)
                               cccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGa                                                                  <  2:4551590/101‑1 (MQ=255)
                                ccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGAt                                                                 <  1:3040070/101‑1 (MQ=255)
                                ccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGAt                                                                 <  1:2450450/101‑1 (MQ=255)
                                ccgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGAt                                                                 <  1:2206363/101‑1 (MQ=255)
                                 cgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATc                                                                <  2:2378841/101‑1 (MQ=255)
                                 cgGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATc                                                                <  2:649055/101‑1 (MQ=255)
                                  ggTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCg                                                               >  1:3828858/1‑101 (MQ=255)
                                  ggTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCg                                                               <  1:816628/101‑1 (MQ=255)
                                  ggTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCg                                                               >  1:3659194/1‑101 (MQ=255)
                                   gTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGc                                                              >  2:3562016/1‑101 (MQ=255)
                                    tCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCTGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGcc                                                             <  2:571074/101‑1 (MQ=255)
                                    tCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGcc                                                             >  2:2010001/1‑101 (MQ=255)
                                     cAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCg                                                            <  1:4314080/101‑1 (MQ=255)
                                      aaTCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCgg                                                           >  2:1836983/1‑100 (MQ=255)
                                       aTCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAg                                                          <  1:2475137/101‑1 (MQ=255)
                                       aTCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAg                                                          <  1:1875007/101‑1 (MQ=255)
                                        tCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGAGCGCCGaag                                                         >  1:4697723/1‑99 (MQ=255)
                                         ctgctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGc                                                        >  2:3041077/1‑101 (MQ=255)
                                         ctgctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGc                                                        >  1:4039924/1‑101 (MQ=255)
                                           gctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCgg                                                      >  2:4326988/1‑101 (MQ=255)
                                            ctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCCGAGCGCCGAGGCggg                                                     >  2:2896850/1‑100 (MQ=255)
                                            ctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCggg                                                     >  2:1674766/1‑100 (MQ=255)
                                            ctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCggg                                                     >  1:1059262/1‑100 (MQ=255)
                                            ctgATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGt                                                     <  2:4055722/101‑1 (MQ=255)
                                              gATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGa                                                   >  1:1333323/1‑101 (MQ=255)
                                                tCGAGACCGCGACCGCCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGAtt                                                 <  2:3890540/101‑1 (MQ=255)
                                                tCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGAtt                                                 <  1:2784591/101‑1 (MQ=255)
                                                  gagaCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGc                                               >  2:61224/1‑101 (MQ=255)
                                                    gaCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGccc                                             <  1:1544436/101‑1 (MQ=255)
                                                     aCCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCa                                            <  1:864129/101‑1 (MQ=255)
                                                      ccGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCaa                                           <  1:788460/101‑1 (MQ=255)
                                                        gcgACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATc                                         <  2:3761633/101‑1 (MQ=255)
                                                         cgACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGCGGCGGGGGTTGCCCAATcc                                        >  1:4403673/1‑101 (MQ=255)
                                                         cgACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATcc                                        <  2:1260603/101‑1 (MQ=255)
                                                          gACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGGTTGCCCAATCCg                                       >  2:1167605/1‑101 (MQ=255)
                                                          gACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGAGATGGAGGCGGGGCAGATCGCCGAGGCCGTGATTGCCCAATCCg                                       >  2:4584714/1‑101 (MQ=255)
                                                            cccccGTGCCCGGCGCCGATCCCGGTGCCGCGCAGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgcc                                     <  1:4083919/98‑1 (MQ=255)
                                                            ccaccGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgcc                                     >  1:1157220/1‑101 (MQ=255)
                                                            ccaccGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCCCCGAGGCGGGGTTTGCCCAAACCacc                                     >  1:89367/1‑101 (MQ=255)
                                                              cccGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgccag                                   <  1:1014218/100‑1 (MQ=255)
                                                              accGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCgccag                                   <  1:939099/101‑1 (MQ=255)
                                                               ccGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCCATCCgccagc                                  >  1:728814/1‑101 (MQ=255)
                                                               ccGTGCCCGGCGCCGATCCCGGCGCCGCGCTAGAACCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCcccagc                                  <  2:762478/101‑1 (MQ=255)
                                                                cGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTTCCCAATCCcccagcc                                 >  2:3522131/1‑101 (MQ=255)
                                                                  tGCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGccagccag                               >  2:3777061/1‑101 (MQ=255)
                                                                   tcgccgagctgATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCcagccagc                              <  1:972873/91‑1 (MQ=255)
                                                                   gCCCGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCcagccagc                              >  1:2341143/1‑101 (MQ=255)
                                                                    cccGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGCGGCGGGGATTGCCCAAACCGCCAGCCAgcg                             >  2:3962413/1‑101 (MQ=255)
                                                                      cGGCGCCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGcgcc                           <  2:2498965/101‑1 (MQ=255)
                                                                         cgcCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCa                        <  2:4747857/101‑1 (MQ=255)
                                                                         cgcCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCa                        >  1:372439/1‑101 (MQ=255)
                                                                          gcCGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCaa                       <  2:4091183/101‑1 (MQ=255)
                                                                            cGATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGc                     <  2:3427892/101‑1 (MQ=255)
                                                                             gATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCt                    >  1:3159346/1‑101 (MQ=255)
                                                                             gATCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCt                    <  2:3251017/101‑1 (MQ=255)
                                                                              aTCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCtg                   <  1:255802/101‑1 (MQ=255)
                                                                              aTCCCGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCtg                   >  1:3422697/1‑101 (MQ=255)
                                                                                cccGGTGCCGCTCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCtgtg                 <  2:1397678/101‑1 (MQ=255)
                                                                                cccGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCtgtg                 <  1:1219986/101‑1 (MQ=255)
                                                                                  cGGTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCCGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTggg               >  1:4733465/1‑101 (MQ=255)
                                                                                   ggTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTgggg              >  1:4641302/1‑100 (MQ=255)
                                                                                   ggTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGga              >  1:4171001/1‑101 (MQ=255)
                                                                                   ggTGCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCAATCCCCCAGCCAGCGCCGCAAGCTGGGGga              >  2:862776/1‑101 (MQ=255)
                                                                                      gCCGCGCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCCGCCAGCGCCCCGAGCTGTGGgagag           >  2:4389784/1‑100 (MQ=255)
                                                                                       ccGCTCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATg          <  2:3198458/101‑1 (MQ=255)
                                                                                        cgcgCTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATgc         <  1:46469/101‑1 (MQ=255)
                                                                                            cTGGAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGa     >  1:3937998/1‑101 (MQ=255)
                                                                                              ggAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAAt   >  1:3075403/1‑101 (MQ=255)
                                                                                               gAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGGGATGCGCGAATc  >  2:1938992/1‑101 (MQ=255)
                                                                                               gAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAATc  >  2:2759052/1‑101 (MQ=255)
                                                                                               gAATCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGGGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAATc  >  1:1628316/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AACCGGCGCTGGCCTGTCCCTATGCGCCCCGCCCGGTCAATCTGCTGATCGAGACCGCGACCACCGTGCCCGGCGCCGATCCCGGTGCCGCGCTGGAAGCCCTGCTTGGCGCGGCGATGGAGGCGGGGCAGATCGCCGAGGCGGTGATTGCCCAATCCGCCAGCCAGCGCCGCAAGCTGTGGGAGATGCGCGAATC  >  NC_014034/1244705‑1244900

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG